Klebsiella virus KpV2811
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D7F139|A0A7D7F139_9CAUD DNA polymerase III beta subunit OS=Klebsiella virus KpV2811 OX=2759464 GN=KpV2811_036 PE=4 SV=1
MM1 pKa = 7.53 NEE3 pKa = 3.46 TDD5 pKa = 3.32 ADD7 pKa = 3.7 LRR9 pKa = 11.84 FYY11 pKa = 10.87 IDD13 pKa = 5.05 LYY15 pKa = 10.09 VDD17 pKa = 3.62 CGFTYY22 pKa = 10.8 DD23 pKa = 4.29 EE24 pKa = 5.08 AEE26 pKa = 4.23 LMAKK30 pKa = 10.09 KK31 pKa = 9.93 LLAMVGVTFDD41 pKa = 3.8 EE42 pKa = 4.59 NNN44 pKa = 3.03
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.846
IPC_protein 3.719
Toseland 3.528
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.452
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.846
Patrickios 1.875
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.782
Protein with the highest isoelectric point:
>tr|A0A7D7JIC8|A0A7D7JIC8_9CAUD Uncharacterized protein OS=Klebsiella virus KpV2811 OX=2759464 GN=KpV2811_047 PE=4 SV=1
MM1 pKa = 7.0 KK2 pKa = 10.18 TSTLTVNIAIRR13 pKa = 11.84 KK14 pKa = 6.56 WCMPLLVILVLLRR27 pKa = 11.84 LPVPRR32 pKa = 11.84 WIYY35 pKa = 9.01 TLEE38 pKa = 4.1 AAPCQRR44 pKa = 4.2
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.545
IPC_protein 10.131
Toseland 10.467
ProMoST 10.833
Dawson 10.599
Bjellqvist 10.306
Wikipedia 10.774
Rodwell 10.877
Grimsley 10.643
Solomon 10.701
Lehninger 10.672
Nozaki 10.526
DTASelect 10.277
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.54
Patrickios 10.804
IPC_peptide 10.701
IPC2_peptide 9.677
IPC2.peptide.svr19 8.625
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
78
0
78
14446
38
1095
185.2
20.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.85 ± 0.906
1.253 ± 0.181
5.829 ± 0.267
6.493 ± 0.282
3.129 ± 0.193
7.185 ± 0.21
1.731 ± 0.183
6.272 ± 0.197
6.265 ± 0.353
7.303 ± 0.173
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.039 ± 0.175
4.624 ± 0.243
3.517 ± 0.269
4.126 ± 0.334
5.517 ± 0.266
6.285 ± 0.271
5.752 ± 0.406
6.625 ± 0.223
1.551 ± 0.146
3.655 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here