Amino acid dipepetide frequency for Gracilibacillus halophilus YIM-C55.5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.266AlaAla: 4.266 ± 0.088
0.596AlaCys: 0.596 ± 0.026
3.564AlaAsp: 3.564 ± 0.072
4.335AlaGlu: 4.335 ± 0.07
3.031AlaPhe: 3.031 ± 0.056
4.396AlaGly: 4.396 ± 0.094
1.278AlaHis: 1.278 ± 0.04
5.904AlaIle: 5.904 ± 0.092
4.084AlaLys: 4.084 ± 0.068
6.343AlaLeu: 6.343 ± 0.093
1.989AlaMet: 1.989 ± 0.051
3.012AlaAsn: 3.012 ± 0.062
1.795AlaPro: 1.795 ± 0.052
2.385AlaGln: 2.385 ± 0.053
2.392AlaArg: 2.392 ± 0.056
4.189AlaSer: 4.189 ± 0.074
3.783AlaThr: 3.783 ± 0.068
4.683AlaVal: 4.683 ± 0.092
0.562AlaTrp: 0.562 ± 0.025
2.376AlaTyr: 2.376 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
0.371CysAla: 0.371 ± 0.023
0.079CysCys: 0.079 ± 0.01
0.388CysAsp: 0.388 ± 0.022
0.367CysGlu: 0.367 ± 0.023
0.298CysPhe: 0.298 ± 0.02
0.607CysGly: 0.607 ± 0.027
0.223CysHis: 0.223 ± 0.016
0.495CysIle: 0.495 ± 0.025
0.275CysLys: 0.275 ± 0.019
0.637CysLeu: 0.637 ± 0.032
0.193CysMet: 0.193 ± 0.016
0.258CysAsn: 0.258 ± 0.018
0.317CysPro: 0.317 ± 0.022
0.265CysGln: 0.265 ± 0.017
0.262CysArg: 0.262 ± 0.018
0.417CysSer: 0.417 ± 0.019
0.322CysThr: 0.322 ± 0.018
0.383CysVal: 0.383 ± 0.021
0.056CysTrp: 0.056 ± 0.008
0.267CysTyr: 0.267 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
3.525AspAla: 3.525 ± 0.066
0.334AspCys: 0.334 ± 0.022
3.349AspAsp: 3.349 ± 0.076
5.32AspGlu: 5.32 ± 0.086
2.527AspPhe: 2.527 ± 0.053
3.553AspGly: 3.553 ± 0.075
1.759AspHis: 1.759 ± 0.046
4.742AspIle: 4.742 ± 0.079
3.084AspLys: 3.084 ± 0.055
4.835AspLeu: 4.835 ± 0.085
1.577AspMet: 1.577 ± 0.045
2.104AspAsn: 2.104 ± 0.051
2.073AspPro: 2.073 ± 0.047
3.68AspGln: 3.68 ± 0.071
2.63AspArg: 2.63 ± 0.059
2.672AspSer: 2.672 ± 0.055
3.017AspThr: 3.017 ± 0.064
4.371AspVal: 4.371 ± 0.075
0.757AspTrp: 0.757 ± 0.037
2.414AspTyr: 2.414 ± 0.052
0.0AspXaa: 0.0 ± 0.0
Glu
5.304GluAla: 5.304 ± 0.095
0.292GluCys: 0.292 ± 0.021
4.471GluAsp: 4.471 ± 0.076
7.439GluGlu: 7.439 ± 0.122
2.201GluPhe: 2.201 ± 0.049
4.149GluGly: 4.149 ± 0.062
1.695GluHis: 1.695 ± 0.048
5.174GluIle: 5.174 ± 0.078
5.835GluLys: 5.835 ± 0.092
6.35GluLeu: 6.35 ± 0.089
2.43GluMet: 2.43 ± 0.053
4.009GluAsn: 4.009 ± 0.087
2.094GluPro: 2.094 ± 0.058
4.334GluGln: 4.334 ± 0.096
3.393GluArg: 3.393 ± 0.072
3.785GluSer: 3.785 ± 0.069
4.415GluThr: 4.415 ± 0.08
5.142GluVal: 5.142 ± 0.079
0.874GluTrp: 0.874 ± 0.037
2.288GluTyr: 2.288 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
2.864PheAla: 2.864 ± 0.071
0.304PheCys: 0.304 ± 0.02
2.635PheAsp: 2.635 ± 0.056
2.725PheGlu: 2.725 ± 0.062
2.401PhePhe: 2.401 ± 0.068
3.092PheGly: 3.092 ± 0.07
1.21PheHis: 1.21 ± 0.04
4.032PheIle: 4.032 ± 0.086
1.743PheLys: 1.743 ± 0.047
4.309PheLeu: 4.309 ± 0.096
1.274PheMet: 1.274 ± 0.043
1.579PheAsn: 1.579 ± 0.048
1.575PhePro: 1.575 ± 0.046
2.126PheGln: 2.126 ± 0.053
1.676PheArg: 1.676 ± 0.039
3.062PheSer: 3.062 ± 0.059
2.631PheThr: 2.631 ± 0.055
3.362PheVal: 3.362 ± 0.074
0.487PheTrp: 0.487 ± 0.03
1.81PheTyr: 1.81 ± 0.051
0.0PheXaa: 0.0 ± 0.0
Gly
4.321GlyAla: 4.321 ± 0.09
0.533GlyCys: 0.533 ± 0.026
3.495GlyAsp: 3.495 ± 0.078
4.507GlyGlu: 4.507 ± 0.076
3.209GlyPhe: 3.209 ± 0.073
4.2GlyGly: 4.2 ± 0.091
1.362GlyHis: 1.362 ± 0.043
5.337GlyIle: 5.337 ± 0.091
4.05GlyLys: 4.05 ± 0.069
5.817GlyLeu: 5.817 ± 0.098
2.104GlyMet: 2.104 ± 0.054
2.632GlyAsn: 2.632 ± 0.059
1.542GlyPro: 1.542 ± 0.048
2.201GlyGln: 2.201 ± 0.047
2.224GlyArg: 2.224 ± 0.059
3.725GlySer: 3.725 ± 0.076
3.685GlyThr: 3.685 ± 0.074
4.726GlyVal: 4.726 ± 0.096
0.713GlyTrp: 0.713 ± 0.033
2.781GlyTyr: 2.781 ± 0.057
0.0GlyXaa: 0.0 ± 0.0
His
1.542HisAla: 1.542 ± 0.047
0.199HisCys: 0.199 ± 0.016
1.334HisAsp: 1.334 ± 0.036
1.596HisGlu: 1.596 ± 0.04
1.225HisPhe: 1.225 ± 0.042
1.438HisGly: 1.438 ± 0.045
0.887HisHis: 0.887 ± 0.038
1.778HisIle: 1.778 ± 0.048
1.072HisLys: 1.072 ± 0.043
2.304HisLeu: 2.304 ± 0.059
0.637HisMet: 0.637 ± 0.027
0.848HisAsn: 0.848 ± 0.032
1.216HisPro: 1.216 ± 0.04
1.577HisGln: 1.577 ± 0.043
1.034HisArg: 1.034 ± 0.038
1.268HisSer: 1.268 ± 0.042
1.325HisThr: 1.325 ± 0.04
1.764HisVal: 1.764 ± 0.043
0.311HisTrp: 0.311 ± 0.019
1.074HisTyr: 1.074 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
5.815IleAla: 5.815 ± 0.109
0.599IleCys: 0.599 ± 0.028
5.281IleAsp: 5.281 ± 0.088
5.818IleGlu: 5.818 ± 0.097
3.383IlePhe: 3.383 ± 0.08
5.945IleGly: 5.945 ± 0.109
2.019IleHis: 2.019 ± 0.052
5.741IleIle: 5.741 ± 0.107
3.772IleLys: 3.772 ± 0.071
6.549IleLeu: 6.549 ± 0.117
1.788IleMet: 1.788 ± 0.051
3.355IleAsn: 3.355 ± 0.067
3.264IlePro: 3.264 ± 0.061
3.671IleGln: 3.671 ± 0.064
3.163IleArg: 3.163 ± 0.075
4.945IleSer: 4.945 ± 0.087
4.596IleThr: 4.596 ± 0.079
5.572IleVal: 5.572 ± 0.106
0.695IleTrp: 0.695 ± 0.029
2.6IleTyr: 2.6 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
3.809LysAla: 3.809 ± 0.07
0.271LysCys: 0.271 ± 0.021
3.462LysAsp: 3.462 ± 0.058
5.759LysGlu: 5.759 ± 0.083
1.542LysPhe: 1.542 ± 0.043
3.638LysGly: 3.638 ± 0.06
1.47LysHis: 1.47 ± 0.046
3.704LysIle: 3.704 ± 0.066
4.858LysLys: 4.858 ± 0.09
4.713LysLeu: 4.713 ± 0.066
1.92LysMet: 1.92 ± 0.05
2.861LysAsn: 2.861 ± 0.059
1.884LysPro: 1.884 ± 0.052
4.087LysGln: 4.087 ± 0.081
3.178LysArg: 3.178 ± 0.06
2.937LysSer: 2.937 ± 0.055
3.122LysThr: 3.122 ± 0.058
3.838LysVal: 3.838 ± 0.073
0.786LysTrp: 0.786 ± 0.028
1.943LysTyr: 1.943 ± 0.046
0.0LysXaa: 0.0 ± 0.0
Leu
6.475LeuAla: 6.475 ± 0.09
0.607LeuCys: 0.607 ± 0.024
5.271LeuAsp: 5.271 ± 0.075
6.241LeuGlu: 6.241 ± 0.094
4.887LeuPhe: 4.887 ± 0.112
5.371LeuGly: 5.371 ± 0.081
2.213LeuHis: 2.213 ± 0.059
6.825LeuIle: 6.825 ± 0.121
5.159LeuLys: 5.159 ± 0.079
9.445LeuLeu: 9.445 ± 0.137
2.373LeuMet: 2.373 ± 0.064
3.841LeuAsn: 3.841 ± 0.076
3.625LeuPro: 3.625 ± 0.071
4.358LeuGln: 4.358 ± 0.096
3.462LeuArg: 3.462 ± 0.063
6.429LeuSer: 6.429 ± 0.093
5.478LeuThr: 5.478 ± 0.081
5.517LeuVal: 5.517 ± 0.085
0.805LeuTrp: 0.805 ± 0.035
3.211LeuTyr: 3.211 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
1.935MetAla: 1.935 ± 0.052
0.136MetCys: 0.136 ± 0.013
1.702MetAsp: 1.702 ± 0.042
2.125MetGlu: 2.125 ± 0.048
1.152MetPhe: 1.152 ± 0.037
1.627MetGly: 1.627 ± 0.046
0.508MetHis: 0.508 ± 0.028
2.516MetIle: 2.516 ± 0.057
2.368MetLys: 2.368 ± 0.048
2.532MetLeu: 2.532 ± 0.059
0.958MetMet: 0.958 ± 0.035
1.831MetAsn: 1.831 ± 0.043
1.014MetPro: 1.014 ± 0.039
1.144MetGln: 1.144 ± 0.038
1.131MetArg: 1.131 ± 0.038
1.667MetSer: 1.667 ± 0.052
1.785MetThr: 1.785 ± 0.046
1.933MetVal: 1.933 ± 0.049
0.196MetTrp: 0.196 ± 0.015
0.831MetTyr: 0.831 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
2.603AsnAla: 2.603 ± 0.053
0.268AsnCys: 0.268 ± 0.018
2.793AsnAsp: 2.793 ± 0.062
3.654AsnGlu: 3.654 ± 0.077
1.555AsnPhe: 1.555 ± 0.047
3.238AsnGly: 3.238 ± 0.073
1.394AsnHis: 1.394 ± 0.042
3.413AsnIle: 3.413 ± 0.07
2.523AsnLys: 2.523 ± 0.06
3.407AsnLeu: 3.407 ± 0.068
1.238AsnMet: 1.238 ± 0.042
1.943AsnAsn: 1.943 ± 0.058
1.89AsnPro: 1.89 ± 0.05
3.288AsnGln: 3.288 ± 0.074
2.123AsnArg: 2.123 ± 0.049
2.095AsnSer: 2.095 ± 0.049
2.212AsnThr: 2.212 ± 0.05
3.163AsnVal: 3.163 ± 0.053
0.631AsnTrp: 0.631 ± 0.029
1.656AsnTyr: 1.656 ± 0.055
0.0AsnXaa: 0.0 ± 0.0
Pro
2.02ProAla: 2.02 ± 0.053
0.185ProCys: 0.185 ± 0.016
2.077ProAsp: 2.077 ± 0.046
2.813ProGlu: 2.813 ± 0.065
1.963ProPhe: 1.963 ± 0.049
2.042ProGly: 2.042 ± 0.058
0.842ProHis: 0.842 ± 0.034
2.93ProIle: 2.93 ± 0.061
1.92ProLys: 1.92 ± 0.044
3.245ProLeu: 3.245 ± 0.054
0.832ProMet: 0.832 ± 0.028
1.814ProAsn: 1.814 ± 0.048
0.879ProPro: 0.879 ± 0.034
1.159ProGln: 1.159 ± 0.041
0.993ProArg: 0.993 ± 0.034
2.284ProSer: 2.284 ± 0.052
2.088ProThr: 2.088 ± 0.044
2.616ProVal: 2.616 ± 0.057
0.346ProTrp: 0.346 ± 0.019
1.467ProTyr: 1.467 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
3.528GlnAla: 3.528 ± 0.066
0.229GlnCys: 0.229 ± 0.019
2.428GlnAsp: 2.428 ± 0.052
3.841GlnGlu: 3.841 ± 0.071
2.018GlnPhe: 2.018 ± 0.053
2.523GlnGly: 2.523 ± 0.057
1.142GlnHis: 1.142 ± 0.039
3.251GlnIle: 3.251 ± 0.057
3.08GlnLys: 3.08 ± 0.073
5.329GlnLeu: 5.329 ± 0.109
1.659GlnMet: 1.659 ± 0.044
2.085GlnAsn: 2.085 ± 0.057
1.726GlnPro: 1.726 ± 0.053
3.133GlnGln: 3.133 ± 0.087
1.941GlnArg: 1.941 ± 0.052
3.248GlnSer: 3.248 ± 0.076
2.928GlnThr: 2.928 ± 0.052
3.064GlnVal: 3.064 ± 0.066
0.636GlnTrp: 0.636 ± 0.029
1.749GlnTyr: 1.749 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
2.211ArgAla: 2.211 ± 0.056
0.222ArgCys: 0.222 ± 0.016
2.219ArgAsp: 2.219 ± 0.047
2.923ArgGlu: 2.923 ± 0.056
1.885ArgPhe: 1.885 ± 0.048
2.147ArgGly: 2.147 ± 0.049
0.965ArgHis: 0.965 ± 0.035
2.988ArgIle: 2.988 ± 0.06
3.048ArgLys: 3.048 ± 0.061
3.995ArgLeu: 3.995 ± 0.068
1.389ArgMet: 1.389 ± 0.034
1.884ArgAsn: 1.884 ± 0.042
1.25ArgPro: 1.25 ± 0.042
1.939ArgGln: 1.939 ± 0.055
1.757ArgArg: 1.757 ± 0.053
2.318ArgSer: 2.318 ± 0.048
2.052ArgThr: 2.052 ± 0.046
2.516ArgVal: 2.516 ± 0.054
0.459ArgTrp: 0.459 ± 0.023
1.766ArgTyr: 1.766 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
3.314SerAla: 3.314 ± 0.078
0.348SerCys: 0.348 ± 0.02
3.406SerAsp: 3.406 ± 0.061
4.047SerGlu: 4.047 ± 0.081
3.276SerPhe: 3.276 ± 0.06
3.941SerGly: 3.941 ± 0.072
1.373SerHis: 1.373 ± 0.043
5.215SerIle: 5.215 ± 0.108
3.238SerLys: 3.238 ± 0.064
5.737SerLeu: 5.737 ± 0.081
1.882SerMet: 1.882 ± 0.05
2.864SerAsn: 2.864 ± 0.06
1.94SerPro: 1.94 ± 0.043
2.443SerGln: 2.443 ± 0.059
2.03SerArg: 2.03 ± 0.046
4.008SerSer: 4.008 ± 0.091
3.331SerThr: 3.331 ± 0.066
4.241SerVal: 4.241 ± 0.073
0.656SerTrp: 0.656 ± 0.03
2.397SerTyr: 2.397 ± 0.049
0.0SerXaa: 0.0 ± 0.0
Thr
3.674ThrAla: 3.674 ± 0.065
0.386ThrCys: 0.386 ± 0.024
3.315ThrAsp: 3.315 ± 0.073
3.846ThrGlu: 3.846 ± 0.073
2.854ThrPhe: 2.854 ± 0.068
3.89ThrGly: 3.89 ± 0.08
1.171ThrHis: 1.171 ± 0.041
5.085ThrIle: 5.085 ± 0.078
3.219ThrLys: 3.219 ± 0.068
5.091ThrLeu: 5.091 ± 0.079
1.554ThrMet: 1.554 ± 0.044
3.011ThrAsn: 3.011 ± 0.064
2.216ThrPro: 2.216 ± 0.051
1.767ThrGln: 1.767 ± 0.048
1.814ThrArg: 1.814 ± 0.048
3.606ThrSer: 3.606 ± 0.072
3.301ThrThr: 3.301 ± 0.074
4.272ThrVal: 4.272 ± 0.063
0.581ThrTrp: 0.581 ± 0.03
2.325ThrTyr: 2.325 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
4.674ValAla: 4.674 ± 0.087
0.541ValCys: 0.541 ± 0.03
4.244ValAsp: 4.244 ± 0.075
5.06ValGlu: 5.06 ± 0.088
3.025ValPhe: 3.025 ± 0.066
4.389ValGly: 4.389 ± 0.089
1.478ValHis: 1.478 ± 0.045
5.987ValIle: 5.987 ± 0.095
3.974ValLys: 3.974 ± 0.065
6.268ValLeu: 6.268 ± 0.079
1.981ValMet: 1.981 ± 0.055
3.142ValAsn: 3.142 ± 0.063
2.416ValPro: 2.416 ± 0.055
2.631ValGln: 2.631 ± 0.055
2.503ValArg: 2.503 ± 0.055
4.455ValSer: 4.455 ± 0.082
4.472ValThr: 4.472 ± 0.075
4.871ValVal: 4.871 ± 0.094
0.619ValTrp: 0.619 ± 0.03
2.399ValTyr: 2.399 ± 0.063
0.0ValXaa: 0.0 ± 0.0
Trp
0.541TrpAla: 0.541 ± 0.029
0.072TrpCys: 0.072 ± 0.009
0.58TrpAsp: 0.58 ± 0.032
0.72TrpGlu: 0.72 ± 0.031
0.56TrpPhe: 0.56 ± 0.023
0.606TrpGly: 0.606 ± 0.03
0.254TrpHis: 0.254 ± 0.018
0.882TrpIle: 0.882 ± 0.032
0.775TrpLys: 0.775 ± 0.035
1.227TrpLeu: 1.227 ± 0.043
0.426TrpMet: 0.426 ± 0.023
0.559TrpAsn: 0.559 ± 0.027
0.255TrpPro: 0.255 ± 0.018
0.539TrpGln: 0.539 ± 0.026
0.445TrpArg: 0.445 ± 0.022
0.618TrpSer: 0.618 ± 0.032
0.555TrpThr: 0.555 ± 0.026
0.632TrpVal: 0.632 ± 0.029
0.167TrpTrp: 0.167 ± 0.013
0.394TrpTyr: 0.394 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.172TyrAla: 2.172 ± 0.058
0.279TyrCys: 0.279 ± 0.019
2.305TyrAsp: 2.305 ± 0.063
2.54TyrGlu: 2.54 ± 0.061
1.904TyrPhe: 1.904 ± 0.051
2.373TyrGly: 2.373 ± 0.052
1.185TyrHis: 1.185 ± 0.043
2.57TyrIle: 2.57 ± 0.058
1.704TyrLys: 1.704 ± 0.047
3.461TyrLeu: 3.461 ± 0.067
0.886TyrMet: 0.886 ± 0.036
1.456TyrAsn: 1.456 ± 0.044
1.544TyrPro: 1.544 ± 0.044
2.812TyrGln: 2.812 ± 0.074
1.785TyrArg: 1.785 ± 0.044
1.956TyrSer: 1.956 ± 0.048
1.881TyrThr: 1.881 ± 0.048
2.465TyrVal: 2.465 ± 0.054
0.471TyrTrp: 0.471 ± 0.024
1.611TyrTyr: 1.611 ± 0.049
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2968 proteins (838553 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski