Smithella sp. SCADC

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Syntrophobacterales; Syntrophaceae; Smithella; unclassified Smithella

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2552 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091F918|A0A091F918_9DELT Branched-chain amino acid ABC transporter ATP-binding protein OS=Smithella sp. SCADC OX=1499107 GN=ER57_15625 PE=4 SV=1
MM1 pKa = 7.73AYY3 pKa = 10.36VITDD7 pKa = 3.16EE8 pKa = 5.53CIACGSCEE16 pKa = 4.16SEE18 pKa = 4.74CPAEE22 pKa = 4.78AISEE26 pKa = 4.31GEE28 pKa = 4.06DD29 pKa = 3.78KK30 pKa = 11.34YY31 pKa = 11.81VIDD34 pKa = 5.8AKK36 pKa = 10.95LCNDD40 pKa = 4.21CGTCVEE46 pKa = 4.28QCPVEE51 pKa = 4.66AIVPGDD57 pKa = 3.5NKK59 pKa = 11.1

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091FAZ4|A0A091FAZ4_9DELT Uncharacterized protein OS=Smithella sp. SCADC OX=1499107 GN=ER57_10530 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 9.08KK3 pKa = 8.39TLTNKK8 pKa = 9.44TNTKK12 pKa = 9.93RR13 pKa = 11.84KK14 pKa = 7.28RR15 pKa = 11.84THH17 pKa = 5.85GFLVRR22 pKa = 11.84MASKK26 pKa = 10.38SGRR29 pKa = 11.84QVLSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.94GRR40 pKa = 11.84KK41 pKa = 8.66RR42 pKa = 11.84LAVV45 pKa = 3.41

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2552

0

2552

732155

28

2539

286.9

32.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.791 ± 0.046

1.24 ± 0.021

5.347 ± 0.031

6.417 ± 0.046

4.492 ± 0.037

6.952 ± 0.045

1.871 ± 0.023

8.099 ± 0.052

7.507 ± 0.051

9.44 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.025

4.362 ± 0.035

3.962 ± 0.029

3.186 ± 0.03

4.887 ± 0.036

5.901 ± 0.037

5.037 ± 0.038

6.459 ± 0.04

1.029 ± 0.019

3.327 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski