Nipponia nippon (Crested ibis) (Ibis nippon)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia;

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091UPV8|A0A091UPV8_NIPNI Peroxisomal carnitine O-octanoyltransferase OS=Nipponia nippon OX=128390 GN=Y956_09329 PE=3 SV=1
LL1 pKa = 7.02GAGALYY7 pKa = 10.02IAEE10 pKa = 4.86GRR12 pKa = 11.84LSWLEE17 pKa = 3.67NSGVGFSLDD26 pKa = 3.46YY27 pKa = 9.24PTISLHH33 pKa = 6.36AVSRR37 pKa = 11.84DD38 pKa = 3.07LNAYY42 pKa = 7.9PWEE45 pKa = 4.19HH46 pKa = 6.86LYY48 pKa = 11.74VMVNAKK54 pKa = 10.31FEE56 pKa = 4.15EE57 pKa = 4.78EE58 pKa = 4.13EE59 pKa = 4.25TKK61 pKa = 10.06EE62 pKa = 3.96APMAEE67 pKa = 4.28GEE69 pKa = 4.22EE70 pKa = 4.57EE71 pKa = 4.24EE72 pKa = 6.08DD73 pKa = 4.5SDD75 pKa = 6.42DD76 pKa = 3.66DD77 pKa = 4.07TEE79 pKa = 5.23PIAEE83 pKa = 4.12FRR85 pKa = 11.84FVPSDD90 pKa = 3.36KK91 pKa = 10.88SALEE95 pKa = 3.9AMFSAMCEE103 pKa = 4.27CQALHH108 pKa = 7.41PDD110 pKa = 4.21PDD112 pKa = 5.77DD113 pKa = 4.05EE114 pKa = 7.61DD115 pKa = 4.46SDD117 pKa = 3.76NDD119 pKa = 3.97YY120 pKa = 11.37EE121 pKa = 4.59GEE123 pKa = 4.16EE124 pKa = 4.41YY125 pKa = 10.56DD126 pKa = 4.49VEE128 pKa = 4.28AHH130 pKa = 6.59EE131 pKa = 4.84LQQGDD136 pKa = 3.21IPTFYY141 pKa = 10.0TYY143 pKa = 11.09EE144 pKa = 4.17EE145 pKa = 4.62GLSHH149 pKa = 6.9LTAEE153 pKa = 4.71GQATLEE159 pKa = 4.11RR160 pKa = 11.84LEE162 pKa = 4.74GMLAQSVSSQYY173 pKa = 11.66NMAGVRR179 pKa = 11.84TEE181 pKa = 3.7DD182 pKa = 3.58SIRR185 pKa = 11.84EE186 pKa = 4.1FEE188 pKa = 5.37DD189 pKa = 3.48GMEE192 pKa = 3.92VDD194 pKa = 3.78IAPVVAGQFEE204 pKa = 4.42DD205 pKa = 5.26AEE207 pKa = 4.32VDD209 pKa = 3.59HH210 pKa = 7.08

Molecular weight: 23.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091VJF4|A0A091VJF4_NIPNI Protein FAM154B (Fragment) OS=Nipponia nippon OX=128390 GN=Y956_09767 PE=3 SV=1
KKK2 pKa = 10.12RR3 pKa = 11.84QGKKK7 pKa = 8.85RR8 pKa = 11.84QGKKK12 pKa = 8.73RR13 pKa = 11.84QGKKK17 pKa = 8.73RR18 pKa = 11.84QGKKK22 pKa = 8.73RR23 pKa = 11.84QGKKK27 pKa = 8.73RR28 pKa = 11.84QGKKK32 pKa = 8.73RR33 pKa = 11.84QGKKK37 pKa = 8.73RR38 pKa = 11.84QGKKK42 pKa = 8.73RR43 pKa = 11.84QGKKK47 pKa = 8.73RR48 pKa = 11.84QGKKK52 pKa = 8.73RR53 pKa = 11.84QGKKK57 pKa = 9.07RR58 pKa = 11.84

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14132

0

14132

6146519

31

6909

434.9

48.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.535 ± 0.018

2.271 ± 0.019

5.029 ± 0.015

7.133 ± 0.027

3.919 ± 0.016

5.969 ± 0.023

2.538 ± 0.011

4.949 ± 0.016

6.346 ± 0.025

9.743 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.227 ± 0.009

4.096 ± 0.015

5.32 ± 0.026

4.634 ± 0.017

5.318 ± 0.017

8.084 ± 0.028

5.37 ± 0.014

6.32 ± 0.017

1.208 ± 0.008

2.988 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski