Macaca fascicularis papillomavirus 3b
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C3PUJ6|C3PUJ6_RHPV1 Replication protein E1 OS=Macaca fascicularis papillomavirus 3b OX=464936 GN=E1 PE=3 SV=1
MM1 pKa = 7.64 IGPGPTLQDD10 pKa = 2.48 IVLEE14 pKa = 4.34 LEE16 pKa = 4.41 PEE18 pKa = 4.28 PVDD21 pKa = 3.87 LRR23 pKa = 11.84 CYY25 pKa = 9.36 EE26 pKa = 4.05 QLDD29 pKa = 3.76 SSEE32 pKa = 5.23 DD33 pKa = 3.54 EE34 pKa = 5.15 DD35 pKa = 6.02 EE36 pKa = 4.73 IDD38 pKa = 4.01 HH39 pKa = 6.59 HH40 pKa = 5.5 QQQDD44 pKa = 3.45 RR45 pKa = 11.84 PDD47 pKa = 3.64 GQEE50 pKa = 3.75 DD51 pKa = 3.28 DD52 pKa = 4.19 GEE54 pKa = 4.32 RR55 pKa = 11.84 FRR57 pKa = 11.84 ILSDD61 pKa = 3.13 CHH63 pKa = 6.41 VCQNTVSLVVHH74 pKa = 5.86 STHH77 pKa = 7.69 DD78 pKa = 3.72 DD79 pKa = 3.43 LHH81 pKa = 8.04 ILEE84 pKa = 5.19 DD85 pKa = 3.96 LLMGTLEE92 pKa = 4.25 IVCPNCAVV100 pKa = 3.11
Molecular weight: 11.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.948
IPC_protein 3.91
Toseland 3.719
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.745
Grimsley 3.63
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.228
Thurlkill 3.757
EMBOSS 3.834
Sillero 4.037
Patrickios 1.036
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.906
Protein with the highest isoelectric point:
>tr|C3PUJ9|C3PUJ9_RHPV1 Probable protein E5 (Fragment) OS=Macaca fascicularis papillomavirus 3b OX=464936 GN=E5 PE=3 SV=1
YYY2 pKa = 9.4 HH3 pKa = 7.05 PNLCLALPSARR14 pKa = 11.84 EEE16 pKa = 3.86 YY17 pKa = 10.79 LLKKK21 pKa = 10.75 LTDDD25 pKa = 4.24 SRR27 pKa = 11.84 PPSPPTPTPLPRR39 pKa = 11.84 KKK41 pKa = 7.75 RR42 pKa = 11.84 GSAHHH47 pKa = 5.47 RR48 pKa = 11.84 QPKKK52 pKa = 9.69 ADDD55 pKa = 3.05 QTPEEE60 pKa = 4.43 TPCGPWTLTAEEE72 pKa = 4.43 HH73 pKa = 7.12 CLEEE77 pKa = 4.22 HHH79 pKa = 6.5 KKK81 pKa = 8.82 PQGTVTVTVRR91 pKa = 11.84 L
Molecular weight: 10.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.084
IPC2_protein 7.995
IPC_protein 7.98
Toseland 7.556
ProMoST 8.697
Dawson 8.551
Bjellqvist 8.99
Wikipedia 8.478
Rodwell 8.565
Grimsley 7.468
Solomon 8.77
Lehninger 8.785
Nozaki 9.326
DTASelect 8.653
Thurlkill 8.712
EMBOSS 8.785
Sillero 9.077
Patrickios 4.329
IPC_peptide 8.756
IPC2_peptide 8.419
IPC2.peptide.svr19 8.373
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2383
86
627
297.9
33.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.588 ± 0.501
3.021 ± 0.754
5.959 ± 0.506
5.455 ± 0.767
3.567 ± 0.554
6.714 ± 0.626
2.937 ± 0.388
3.945 ± 0.442
4.784 ± 0.783
8.98 ± 1.142
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.762 ± 0.278
3.189 ± 0.556
6.546 ± 1.316
4.448 ± 0.418
5.539 ± 0.593
6.882 ± 0.659
6.924 ± 0.784
7.595 ± 0.475
1.553 ± 0.289
3.609 ± 0.396
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here