Cereal yellow dwarf virus RPS
Average proteome isoelectric point is 8.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9IZF6|Q9IZF6_9LUTE Serine protease OS=Cereal yellow dwarf virus RPS OX=228582 PE=4 SV=1
MM1 pKa = 7.45 GPKK4 pKa = 9.78 KK5 pKa = 10.62 LSFSVFLWLMCFLSFSQGNHH25 pKa = 4.7 TGMATGSDD33 pKa = 4.08 FPSPTTLIYY42 pKa = 10.85 SGGVWPLVTPHH53 pKa = 6.71 SSPVAGEE60 pKa = 4.24 KK61 pKa = 9.43 CTLTCAPCPVRR72 pKa = 11.84 SITEE76 pKa = 3.73 PSYY79 pKa = 11.53 KK80 pKa = 10.24 EE81 pKa = 4.41 LLQQLWVKK89 pKa = 10.62 AWLDD93 pKa = 3.46 SRR95 pKa = 11.84 ALCFTAIDD103 pKa = 3.61 SSSRR107 pKa = 11.84 FLSHH111 pKa = 7.38 AYY113 pKa = 9.4 EE114 pKa = 4.38 LSSATARR121 pKa = 11.84 ALFGQVLWFAVYY133 pKa = 10.07 LWTNVLLQTARR144 pKa = 11.84 IVCSVVASYY153 pKa = 10.77 YY154 pKa = 10.53 LQVASLISLGITTSWIYY171 pKa = 10.82 KK172 pKa = 7.94 ILRR175 pKa = 11.84 WTFGTLPASLCIRR188 pKa = 11.84 LGKK191 pKa = 9.88 SICRR195 pKa = 11.84 VLTCRR200 pKa = 11.84 KK201 pKa = 9.02 FFNEE205 pKa = 3.67 KK206 pKa = 10.02 SVAGFDD212 pKa = 4.07 SYY214 pKa = 11.54 SIPQSPPKK222 pKa = 10.26 KK223 pKa = 10.28 SVITLRR229 pKa = 11.84 RR230 pKa = 11.84 ADD232 pKa = 3.52 KK233 pKa = 9.14 THH235 pKa = 6.38 VGFAVCIRR243 pKa = 11.84 LFNNSNALVTSEE255 pKa = 4.3 HH256 pKa = 6.28 NLRR259 pKa = 11.84 EE260 pKa = 3.73 ADD262 pKa = 3.23 AFYY265 pKa = 11.08 SPRR268 pKa = 11.84 TGRR271 pKa = 11.84 AIKK274 pKa = 10.18 LAEE277 pKa = 4.14 FKK279 pKa = 11.12 VIFKK283 pKa = 10.77 DD284 pKa = 3.1 ADD286 pKa = 3.73 LDD288 pKa = 3.97 VAIVAGPDD296 pKa = 2.87 NWEE299 pKa = 4.06 SAFGCGSVHH308 pKa = 6.19 FTTHH312 pKa = 6.8 DD313 pKa = 3.46 RR314 pKa = 11.84 LAKK317 pKa = 10.27 CPAQIYY323 pKa = 10.35 VIDD326 pKa = 4.45 GEE328 pKa = 4.21 DD329 pKa = 3.25 WRR331 pKa = 11.84 AHH333 pKa = 4.59 SAKK336 pKa = 10.47 VVGHH340 pKa = 6.66 FDD342 pKa = 2.95 NFAQVLSNTKK352 pKa = 9.88 PGFSGAGYY360 pKa = 9.22 FHH362 pKa = 7.39 GKK364 pKa = 7.28 TLLGVHH370 pKa = 6.77 KK371 pKa = 10.32 GHH373 pKa = 7.52 AGKK376 pKa = 10.24 DD377 pKa = 3.49 YY378 pKa = 10.82 NFNLMAPLPAIPGLTSPKK396 pKa = 10.3 YY397 pKa = 9.9 EE398 pKa = 4.26 IEE400 pKa = 4.06 SDD402 pKa = 3.8 PPQGLIFPSEE412 pKa = 4.04 VAEE415 pKa = 4.86 EE416 pKa = 3.97 ITKK419 pKa = 9.35 TIQSVYY425 pKa = 11.22 NDD427 pKa = 3.56 FLKK430 pKa = 10.66 LDD432 pKa = 3.82 KK433 pKa = 10.85 SIHH436 pKa = 6.08 SKK438 pKa = 9.7 GKK440 pKa = 7.18 EE441 pKa = 3.84 WKK443 pKa = 10.68 GGVAWADD450 pKa = 3.59 LEE452 pKa = 4.74 DD453 pKa = 3.89 EE454 pKa = 4.24 VRR456 pKa = 11.84 KK457 pKa = 10.1 VGKK460 pKa = 9.79 RR461 pKa = 11.84 QGGRR465 pKa = 11.84 VRR467 pKa = 11.84 RR468 pKa = 11.84 TNRR471 pKa = 11.84 STKK474 pKa = 9.59 SQGRR478 pKa = 11.84 YY479 pKa = 8.75 KK480 pKa = 10.53 RR481 pKa = 11.84 NKK483 pKa = 9.29 EE484 pKa = 3.97 SCCTFFAEE492 pKa = 4.6 GTAAPRR498 pKa = 11.84 CIPTHH503 pKa = 4.67 THH505 pKa = 6.24 NLDD508 pKa = 3.98 DD509 pKa = 4.07 NPMFSRR515 pKa = 11.84 CYY517 pKa = 10.56 CNIPGHH523 pKa = 6.49 KK524 pKa = 10.02 CPFWDD529 pKa = 3.61 VSARR533 pKa = 11.84 RR534 pKa = 11.84 DD535 pKa = 3.3 AEE537 pKa = 4.09 LNEE540 pKa = 4.19 FARR543 pKa = 11.84 TEE545 pKa = 3.98 DD546 pKa = 2.98 RR547 pKa = 11.84 HH548 pKa = 7.3 VEE550 pKa = 3.78 DD551 pKa = 5.41 RR552 pKa = 11.84 EE553 pKa = 3.62 IDD555 pKa = 3.68 NRR557 pKa = 11.84 SGRR560 pKa = 11.84 CTSSEE565 pKa = 3.92 EE566 pKa = 3.89 TSRR569 pKa = 11.84 QARR572 pKa = 11.84 LQQEE576 pKa = 3.9 TRR578 pKa = 11.84 AWQQYY583 pKa = 8.62 FADD586 pKa = 4.01 VYY588 pKa = 8.55 TWEE591 pKa = 4.52 ISTSEE596 pKa = 3.94 QEE598 pKa = 4.06 VPGFQRR604 pKa = 11.84 VGKK607 pKa = 10.19 LSPQYY612 pKa = 10.35 YY613 pKa = 9.04 PRR615 pKa = 11.84 ARR617 pKa = 11.84 STTAWGEE624 pKa = 4.12 RR625 pKa = 11.84 LCAEE629 pKa = 4.48 HH630 pKa = 7.31 PLLGEE635 pKa = 4.01 KK636 pKa = 9.56 TKK638 pKa = 11.02 GFGWPAVGATAEE650 pKa = 4.28 LTSLRR655 pKa = 11.84 LQAARR660 pKa = 11.84 WLEE663 pKa = 3.8 RR664 pKa = 11.84 SEE666 pKa = 4.28 SAKK669 pKa = 10.39 IPSDD673 pKa = 3.28 AARR676 pKa = 11.84 KK677 pKa = 9.11 NVIDD681 pKa = 4.02 RR682 pKa = 11.84 TVQAYY687 pKa = 8.96 SNCKK691 pKa = 9.05 TNVPRR696 pKa = 11.84 CTRR699 pKa = 11.84 DD700 pKa = 3.14 QLNWDD705 pKa = 3.97 DD706 pKa = 4.37 FRR708 pKa = 11.84 IDD710 pKa = 3.29 FLEE713 pKa = 4.94 AIKK716 pKa = 10.62 SLQLDD721 pKa = 3.75 AGVGIPMITAGLPTHH736 pKa = 6.9 RR737 pKa = 11.84 GWVEE741 pKa = 4.24 DD742 pKa = 4.09 PDD744 pKa = 4.35 LLPVLARR751 pKa = 11.84 LTFDD755 pKa = 4.64 RR756 pKa = 11.84 LLTMSKK762 pKa = 10.8 ASLEE766 pKa = 4.33 TRR768 pKa = 11.84 SPEE771 pKa = 3.89 QLVKK775 pKa = 10.99 EE776 pKa = 4.26 NLCDD780 pKa = 4.79 PIRR783 pKa = 11.84 LFVKK787 pKa = 10.15 QEE789 pKa = 3.56 PHH791 pKa = 6.14 KK792 pKa = 10.47 QSKK795 pKa = 10.18 LDD797 pKa = 3.38 EE798 pKa = 4.27 GRR800 pKa = 11.84 YY801 pKa = 8.96 RR802 pKa = 11.84 LIMSVSLIDD811 pKa = 3.51 QLVARR816 pKa = 11.84 VLFQAQNKK824 pKa = 9.79 SEE826 pKa = 4.03 IALWSAIPSKK836 pKa = 10.64 PGFGLSTEE844 pKa = 4.32 DD845 pKa = 3.64 QVEE848 pKa = 4.23 SFINVLADD856 pKa = 3.24 TAGARR861 pKa = 11.84 PEE863 pKa = 4.31 EE864 pKa = 4.68 ICDD867 pKa = 3.04 KK868 pKa = 10.61 WRR870 pKa = 11.84 DD871 pKa = 3.63 LLVPTDD877 pKa = 3.84 CSGFDD882 pKa = 3.34 WSVSDD887 pKa = 4.78 WMLADD892 pKa = 4.01 DD893 pKa = 3.87 MEE895 pKa = 4.42 VRR897 pKa = 11.84 NRR899 pKa = 11.84 LTIDD903 pKa = 3.34 CNEE906 pKa = 4.17 LTRR909 pKa = 11.84 HH910 pKa = 5.95 LRR912 pKa = 11.84 AVWLQGISNSVLCLSDD928 pKa = 3.27 GTMLSQVKK936 pKa = 10.09 PGVQKK941 pKa = 10.66 SGSYY945 pKa = 8.27 NTSSTNSRR953 pKa = 11.84 IRR955 pKa = 11.84 VMAAYY960 pKa = 9.92 HH961 pKa = 6.58 CGASWAIAMGDD972 pKa = 3.84 DD973 pKa = 4.05 ALEE976 pKa = 4.56 APDD979 pKa = 4.44 TDD981 pKa = 3.56 LSKK984 pKa = 11.61 YY985 pKa = 10.03 KK986 pKa = 10.67 DD987 pKa = 3.37 LGFKK991 pKa = 10.77 VEE993 pKa = 4.04 VSKK996 pKa = 10.86 EE997 pKa = 3.96 LEE999 pKa = 4.1 FCSHH1003 pKa = 6.51 IFKK1006 pKa = 10.88 SPTLAIPVNANKK1018 pKa = 9.37 MLYY1021 pKa = 10.12 RR1022 pKa = 11.84 LIHH1025 pKa = 6.62 GYY1027 pKa = 9.99 NPEE1030 pKa = 4.27 CGNAEE1035 pKa = 4.24 VIVNYY1040 pKa = 10.48 LNAASSVLHH1049 pKa = 6.15 EE1050 pKa = 4.61 LRR1052 pKa = 11.84 HH1053 pKa = 5.37 DD1054 pKa = 3.9 QEE1056 pKa = 4.61 LCALLHH1062 pKa = 5.68 MWLVSGITTKK1072 pKa = 11.17 DD1073 pKa = 3.01 NN1074 pKa = 3.52
Molecular weight: 120.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.941
IPC2_protein 7.263
IPC_protein 7.059
Toseland 6.795
ProMoST 7.688
Dawson 7.761
Bjellqvist 8.258
Wikipedia 7.673
Rodwell 7.79
Grimsley 6.854
Solomon 7.775
Lehninger 7.805
Nozaki 8.551
DTASelect 7.951
Thurlkill 7.995
EMBOSS 7.995
Sillero 8.346
Patrickios 4.304
IPC_peptide 7.775
IPC2_peptide 7.41
IPC2.peptide.svr19 7.234
Protein with the highest isoelectric point:
>tr|Q9IZF4|Q9IZF4_9LUTE Readthrough protein OS=Cereal yellow dwarf virus RPS OX=228582 PE=3 SV=1
MM1 pKa = 6.73 STVVLRR7 pKa = 11.84 SNGNGSRR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 QRR19 pKa = 11.84 VARR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 PAVRR28 pKa = 11.84 TQPVVVVTPNGPARR42 pKa = 11.84 RR43 pKa = 11.84 GRR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 GPARR51 pKa = 11.84 PRR53 pKa = 11.84 RR54 pKa = 11.84 RR55 pKa = 11.84 RR56 pKa = 11.84 TPGLGGGGRR65 pKa = 11.84 GEE67 pKa = 3.99 TFVFTKK73 pKa = 10.68 DD74 pKa = 3.19 SLAGNSSGSITFGPSLSDD92 pKa = 3.4 YY93 pKa = 10.75 PAFQNGVLKK102 pKa = 10.51 AYY104 pKa = 10.18 HH105 pKa = 6.61 EE106 pKa = 4.26 YY107 pKa = 10.77 KK108 pKa = 9.46 ITGCILQFVSEE119 pKa = 4.53 ASSTAAGSIAYY130 pKa = 9.34 EE131 pKa = 4.26 LDD133 pKa = 3.41 PHH135 pKa = 6.82 CKK137 pKa = 9.42 ISSLASTNNKK147 pKa = 7.78 FTITKK152 pKa = 8.31 TGARR156 pKa = 11.84 SFPAKK161 pKa = 9.68 MINGLEE167 pKa = 3.96 WHH169 pKa = 7.13 PSDD172 pKa = 4.26 EE173 pKa = 4.26 DD174 pKa = 3.37 QFRR177 pKa = 11.84 ILYY180 pKa = 8.98 KK181 pKa = 10.99 GNGASSVAGSFKK193 pKa = 9.64 ITLRR197 pKa = 11.84 VQLQNPKK204 pKa = 10.5
Molecular weight: 22.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.19
IPC2_protein 10.145
IPC_protein 11.374
Toseland 11.506
ProMoST 11.93
Dawson 11.52
Bjellqvist 11.477
Wikipedia 11.959
Rodwell 11.403
Grimsley 11.564
Solomon 11.959
Lehninger 11.871
Nozaki 11.506
DTASelect 11.477
Thurlkill 11.506
EMBOSS 11.989
Sillero 11.506
Patrickios 11.125
IPC_peptide 11.974
IPC2_peptide 10.935
IPC2.peptide.svr19 9.217
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2939
153
1074
489.8
54.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.098 ± 0.337
1.905 ± 0.513
4.491 ± 0.489
4.798 ± 0.488
3.981 ± 0.306
7.043 ± 0.587
2.246 ± 0.271
4.627 ± 0.186
5.104 ± 0.627
8.302 ± 1.088
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.327 ± 0.176
3.641 ± 0.461
6.363 ± 0.776
3.879 ± 0.439
6.635 ± 0.869
10.174 ± 0.751
6.227 ± 0.257
6.397 ± 0.111
1.871 ± 0.297
2.858 ± 0.407
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here