Beihai barnacle virus 13
Average proteome isoelectric point is 7.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KLI1|A0A1L3KLI1_9VIRU RdRp OS=Beihai barnacle virus 13 OX=1922357 PE=4 SV=1
MM1 pKa = 7.66 DD2 pKa = 4.59 TSTSQPSDD10 pKa = 3.44 EE11 pKa = 4.79 RR12 pKa = 11.84 EE13 pKa = 3.92 SAPTHH18 pKa = 5.79 FRR20 pKa = 11.84 SSSNNKK26 pKa = 9.09 QNSTNQSGRR35 pKa = 11.84 GGARR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 GRR43 pKa = 11.84 ASSEE47 pKa = 3.66 KK48 pKa = 10.32 HH49 pKa = 5.04 NEE51 pKa = 3.64 AMRR54 pKa = 11.84 KK55 pKa = 7.57 FHH57 pKa = 7.2 GPSQLQAYY65 pKa = 7.93 RR66 pKa = 11.84 AQLASNNFLDD76 pKa = 5.1 LAEE79 pKa = 5.33 LSQQLASLASASTPLTVKK97 pKa = 10.76 LPATTRR103 pKa = 11.84 GLAEE107 pKa = 4.17 AVLGGYY113 pKa = 10.25 HH114 pKa = 5.5 MAFRR118 pKa = 11.84 LTKK121 pKa = 10.13 EE122 pKa = 4.07 PPISLNAFYY131 pKa = 10.58 RR132 pKa = 11.84 VTLAQFEE139 pKa = 4.39 LQAIRR144 pKa = 11.84 SQLRR148 pKa = 11.84 DD149 pKa = 3.41 HH150 pKa = 6.86 NISLRR155 pKa = 11.84 QYY157 pKa = 9.62 HH158 pKa = 5.45 YY159 pKa = 11.03 EE160 pKa = 3.95 EE161 pKa = 5.15 FFAKK165 pKa = 10.14 YY166 pKa = 10.57 ASFEE170 pKa = 4.39 QIVAAHH176 pKa = 7.0 PATLKK181 pKa = 10.93 LVVAVIEE188 pKa = 4.25 QYY190 pKa = 9.96 GTVSVGGKK198 pKa = 7.82 TYY200 pKa = 11.09 SPFIVDD206 pKa = 4.9 SNVDD210 pKa = 2.87 WSVEE214 pKa = 3.52 VRR216 pKa = 11.84 ARR218 pKa = 11.84 RR219 pKa = 11.84 RR220 pKa = 11.84 GHH222 pKa = 6.4 RR223 pKa = 11.84 ATGASTSALPHH234 pKa = 6.3 RR235 pKa = 11.84 VLEE238 pKa = 4.82 DD239 pKa = 3.16 GGEE242 pKa = 4.27 RR243 pKa = 11.84 ILEE246 pKa = 4.11 VRR248 pKa = 11.84 KK249 pKa = 10.62 GMGPDD254 pKa = 3.55 PYY256 pKa = 10.81 TVTFRR261 pKa = 11.84 NLEE264 pKa = 3.87 NVVRR268 pKa = 11.84 VMSDD272 pKa = 3.1 EE273 pKa = 4.03 NSVLEE278 pKa = 3.94 GRR280 pKa = 11.84 KK281 pKa = 8.53 WFVEE285 pKa = 3.83 NNPLPGARR293 pKa = 11.84 YY294 pKa = 9.24 DD295 pKa = 4.45 DD296 pKa = 4.67 RR297 pKa = 11.84 YY298 pKa = 11.29 VLQNPDD304 pKa = 4.36 EE305 pKa = 4.37 IWPPNYY311 pKa = 9.54 TDD313 pKa = 4.29 ASLSHH318 pKa = 6.74 EE319 pKa = 4.36 FQLLEE324 pKa = 3.79 AWISRR329 pKa = 11.84 LSGKK333 pKa = 10.2 FSYY336 pKa = 9.43 LTGRR340 pKa = 11.84 LSYY343 pKa = 10.4 SGKK346 pKa = 10.15 GSEE349 pKa = 4.16 AMSVGTLTDD358 pKa = 3.1 SHH360 pKa = 6.87 IATTLPQHH368 pKa = 4.93 VTISEE373 pKa = 4.32 SEE375 pKa = 4.01 TNYY378 pKa = 11.3 ALDD381 pKa = 4.53 IISDD385 pKa = 3.6 QSMLLGTLSRR395 pKa = 11.84 WRR397 pKa = 11.84 EE398 pKa = 3.66 YY399 pKa = 10.31 PDD401 pKa = 3.77 YY402 pKa = 11.33 SRR404 pKa = 11.84 LPDD407 pKa = 3.35 NVTTVDD413 pKa = 3.27 PRR415 pKa = 11.84 LYY417 pKa = 10.3 EE418 pKa = 4.12 INSQSGAVRR427 pKa = 11.84 KK428 pKa = 9.5 IVADD432 pKa = 3.33 WHH434 pKa = 6.33 ALTAAVLARR443 pKa = 11.84 PVV445 pKa = 2.95
Molecular weight: 49.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.315
IPC2_protein 7.424
IPC_protein 7.966
Toseland 8.273
ProMoST 7.951
Dawson 8.39
Bjellqvist 7.922
Wikipedia 8.551
Rodwell 8.346
Grimsley 8.419
Solomon 8.712
Lehninger 8.639
Nozaki 8.009
DTASelect 8.273
Thurlkill 8.273
EMBOSS 8.58
Sillero 8.375
Patrickios 4.469
IPC_peptide 8.682
IPC2_peptide 7.38
IPC2.peptide.svr19 7.582
Protein with the highest isoelectric point:
>tr|A0A1L3KLI1|A0A1L3KLI1_9VIRU RdRp OS=Beihai barnacle virus 13 OX=1922357 PE=4 SV=1
MM1 pKa = 7.47 FSLPRR6 pKa = 11.84 GKK8 pKa = 10.93 GFGQYY13 pKa = 9.76 HH14 pKa = 5.85 AGEE17 pKa = 4.35 RR18 pKa = 11.84 RR19 pKa = 11.84 QTPYY23 pKa = 10.71 LPEE26 pKa = 4.0 ITDD29 pKa = 3.42 IPYY32 pKa = 10.07 HH33 pKa = 6.55 RR34 pKa = 11.84 SAVTLSAIRR43 pKa = 11.84 SDD45 pKa = 3.6 LKK47 pKa = 11.21 QFGTTQKK54 pKa = 10.25 IEE56 pKa = 3.94 YY57 pKa = 8.94 TPTLKK62 pKa = 10.75 AAIADD67 pKa = 3.79 AYY69 pKa = 10.13 KK70 pKa = 10.55 EE71 pKa = 3.99 FGIGDD76 pKa = 3.76 VKK78 pKa = 10.37 MLHH81 pKa = 6.63 LNDD84 pKa = 2.84 VMTYY88 pKa = 10.12 KK89 pKa = 10.84 GVDD92 pKa = 2.44 IWNRR96 pKa = 11.84 SPGLPWRR103 pKa = 11.84 QHH105 pKa = 4.56 GYY107 pKa = 6.99 KK108 pKa = 9.74 TKK110 pKa = 11.05 GEE112 pKa = 4.36 VVSDD116 pKa = 3.58 MDD118 pKa = 3.75 AQRR121 pKa = 11.84 SIRR124 pKa = 11.84 WFWHH128 pKa = 5.95 RR129 pKa = 11.84 IKK131 pKa = 10.97 DD132 pKa = 3.99 GAQMRR137 pKa = 11.84 APDD140 pKa = 3.86 CSAFTRR146 pKa = 11.84 SHH148 pKa = 4.78 VCKK151 pKa = 10.62 VGEE154 pKa = 4.18 EE155 pKa = 4.0 KK156 pKa = 10.86 VRR158 pKa = 11.84 AVWGYY163 pKa = 10.21 PMTITMGEE171 pKa = 4.02 AVFAIPLIEE180 pKa = 4.44 AYY182 pKa = 9.26 KK183 pKa = 10.91 ARR185 pKa = 11.84 GSPIAYY191 pKa = 9.36 GYY193 pKa = 9.05 EE194 pKa = 3.69 ISVGGARR201 pKa = 11.84 KK202 pKa = 9.55 IFRR205 pKa = 11.84 DD206 pKa = 3.49 MQTSEE211 pKa = 4.5 FKK213 pKa = 10.83 AALDD217 pKa = 3.92 LEE219 pKa = 4.98 SFDD222 pKa = 4.53 KK223 pKa = 10.66 RR224 pKa = 11.84 VPRR227 pKa = 11.84 QLIEE231 pKa = 3.78 HH232 pKa = 6.93 AFDD235 pKa = 4.64 ILAQNINFMEE245 pKa = 4.05 YY246 pKa = 9.32 RR247 pKa = 11.84 DD248 pKa = 3.79 RR249 pKa = 11.84 GIADD253 pKa = 3.07 VRR255 pKa = 11.84 RR256 pKa = 11.84 NIRR259 pKa = 11.84 MWEE262 pKa = 3.8 YY263 pKa = 10.56 LKK265 pKa = 10.67 QYY267 pKa = 10.78 FLDD270 pKa = 3.93 TPVRR274 pKa = 11.84 LCTGEE279 pKa = 4.85 RR280 pKa = 11.84 YY281 pKa = 9.64 LKK283 pKa = 10.67 RR284 pKa = 11.84 GGVPSGSYY292 pKa = 7.81 FTQLVDD298 pKa = 4.59 SIVNYY303 pKa = 10.24 ILVVWLAYY311 pKa = 10.04 EE312 pKa = 4.1 LTGKK316 pKa = 9.86 KK317 pKa = 9.52 PRR319 pKa = 11.84 YY320 pKa = 8.34 LKK322 pKa = 11.17 VMGDD326 pKa = 3.68 DD327 pKa = 4.55 SIAGLDD333 pKa = 3.88 SPFHH337 pKa = 6.29 LHH339 pKa = 6.36 EE340 pKa = 5.25 ADD342 pKa = 3.28 ALLRR346 pKa = 11.84 TVGMKK351 pKa = 10.82 LNVPKK356 pKa = 10.66 SILSKK361 pKa = 10.88 DD362 pKa = 3.68 VADD365 pKa = 4.15 LTFLGYY371 pKa = 10.41 KK372 pKa = 9.12 INNGIPSRR380 pKa = 11.84 PFDD383 pKa = 3.17 KK384 pKa = 10.56 WIASLVYY391 pKa = 10.31 PEE393 pKa = 5.08 TEE395 pKa = 3.99 DD396 pKa = 4.77 EE397 pKa = 4.31 SWDD400 pKa = 3.85 DD401 pKa = 3.37 VATRR405 pKa = 11.84 ALGLLYY411 pKa = 10.54 ANCGVDD417 pKa = 3.26 SSFDD421 pKa = 3.48 RR422 pKa = 11.84 LCRR425 pKa = 11.84 HH426 pKa = 7.13 IINFKK431 pKa = 10.61 PFDD434 pKa = 3.66 LHH436 pKa = 7.62 ISRR439 pKa = 11.84 SLSRR443 pKa = 11.84 LLWSLGVTGLDD454 pKa = 3.14 KK455 pKa = 10.96 AVPSSLDD462 pKa = 3.32 FYY464 pKa = 11.63 LRR466 pKa = 11.84 LLL468 pKa = 3.98
Molecular weight: 53.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.427
IPC2_protein 8.419
IPC_protein 8.331
Toseland 8.785
ProMoST 8.785
Dawson 9.165
Bjellqvist 9.063
Wikipedia 9.355
Rodwell 9.282
Grimsley 9.194
Solomon 9.238
Lehninger 9.18
Nozaki 9.077
DTASelect 8.975
Thurlkill 9.033
EMBOSS 9.282
Sillero 9.209
Patrickios 4.482
IPC_peptide 9.224
IPC2_peptide 7.775
IPC2.peptide.svr19 7.677
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
913
445
468
456.5
51.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.996 ± 0.698
0.548 ± 0.385
5.805 ± 0.921
5.367 ± 0.65
3.834 ± 0.483
6.572 ± 0.513
2.629 ± 0.206
4.929 ± 1.095
4.491 ± 1.103
9.529 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.081 ± 0.357
3.395 ± 0.773
4.819 ± 0.088
3.395 ± 0.615
7.777 ± 0.22
8.543 ± 1.419
5.586 ± 0.654
6.462 ± 0.196
1.752 ± 0.284
4.491 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here