Vibrio phage Vp_R1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 5.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 147 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5BQ58|A0A2H5BQ58_9CAUD Uncharacterized protein OS=Vibrio phage Vp_R1 OX=2059867 GN=VPR_107 PE=4 SV=1
MM1 pKa = 7.29GLEE4 pKa = 3.98LVIDD8 pKa = 4.49GVSRR12 pKa = 11.84SVGGQLWGEE21 pKa = 3.99VSKK24 pKa = 11.48VIDD27 pKa = 3.71AQYY30 pKa = 11.19SSNSDD35 pKa = 3.28SGVSFVYY42 pKa = 10.83GEE44 pKa = 3.73QKK46 pKa = 11.15YY47 pKa = 10.58NLFEE51 pKa = 5.24CDD53 pKa = 3.39NEE55 pKa = 4.23LVLSVNCRR63 pKa = 11.84TSLSHH68 pKa = 6.2ITDD71 pKa = 4.75FYY73 pKa = 10.35GTDD76 pKa = 3.22VDD78 pKa = 5.37SVLLSAGLLL87 pKa = 3.77

Molecular weight: 9.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5BQL5|A0A2H5BQL5_9CAUD Uncharacterized protein OS=Vibrio phage Vp_R1 OX=2059867 GN=VPR_082 PE=4 SV=1
MM1 pKa = 7.09PRR3 pKa = 11.84KK4 pKa = 10.0VRR6 pKa = 11.84DD7 pKa = 3.43YY8 pKa = 10.1KK9 pKa = 9.95QEE11 pKa = 3.86RR12 pKa = 11.84KK13 pKa = 7.23TAIARR18 pKa = 11.84GEE20 pKa = 4.15TGVGSKK26 pKa = 10.46SGDD29 pKa = 3.39ATRR32 pKa = 11.84HH33 pKa = 4.92RR34 pKa = 11.84ARR36 pKa = 11.84RR37 pKa = 11.84QMEE40 pKa = 4.35KK41 pKa = 10.52KK42 pKa = 10.52VGALSSDD49 pKa = 2.8KK50 pKa = 11.18HH51 pKa = 6.94VDD53 pKa = 3.04HH54 pKa = 7.18KK55 pKa = 11.29KK56 pKa = 11.01GIGAGNSPSNLRR68 pKa = 11.84VRR70 pKa = 11.84PASDD74 pKa = 2.86NMAHH78 pKa = 7.45GGRR81 pKa = 11.84KK82 pKa = 9.27GSKK85 pKa = 9.03KK86 pKa = 10.26GKK88 pKa = 9.14SAGGRR93 pKa = 11.84KK94 pKa = 8.98GALSKK99 pKa = 10.91KK100 pKa = 9.95KK101 pKa = 10.5

Molecular weight: 10.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

147

0

147

30786

39

2214

209.4

23.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.055 ± 0.301

1.134 ± 0.12

6.954 ± 0.19

8.283 ± 0.317

4.158 ± 0.184

7.37 ± 0.263

2.02 ± 0.156

5.863 ± 0.172

6.727 ± 0.348

7.568 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.693 ± 0.133

4.924 ± 0.161

3.823 ± 0.151

2.888 ± 0.133

4.46 ± 0.148

7.097 ± 0.236

5.535 ± 0.176

7.104 ± 0.165

1.397 ± 0.128

3.947 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski