Sewage-associated circular DNA virus-3

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075IZN1|A0A075IZN1_9VIRU Putative capsid protein OS=Sewage-associated circular DNA virus-3 OX=1519392 PE=4 SV=1
MM1 pKa = 7.59VYY3 pKa = 9.32TFSFTYY9 pKa = 8.33PQCSLEE15 pKa = 4.04RR16 pKa = 11.84TEE18 pKa = 5.91LRR20 pKa = 11.84DD21 pKa = 3.97SLIQRR26 pKa = 11.84VNPEE30 pKa = 3.68KK31 pKa = 10.88YY32 pKa = 10.29LIARR36 pKa = 11.84EE37 pKa = 3.94QHH39 pKa = 6.07SDD41 pKa = 3.23GGLHH45 pKa = 5.95LHH47 pKa = 7.3AYY49 pKa = 9.54LHH51 pKa = 6.2FGRR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84FTSADD62 pKa = 3.26AFDD65 pKa = 4.01VDD67 pKa = 4.45GFHH70 pKa = 7.75PNIQKK75 pKa = 9.72PRR77 pKa = 11.84SARR80 pKa = 11.84NVIAYY85 pKa = 8.93CSKK88 pKa = 10.99EE89 pKa = 4.03DD90 pKa = 3.97TEE92 pKa = 4.28PMANFDD98 pKa = 4.05YY99 pKa = 11.08TSGEE103 pKa = 4.2PNGGWSEE110 pKa = 3.93ILGRR114 pKa = 11.84TSSKK118 pKa = 11.33DD119 pKa = 3.21EE120 pKa = 3.96FLEE123 pKa = 4.05EE124 pKa = 4.21VRR126 pKa = 11.84THH128 pKa = 6.63FPRR131 pKa = 11.84DD132 pKa = 3.42YY133 pKa = 10.89VLSLEE138 pKa = 4.2RR139 pKa = 11.84LLFFCEE145 pKa = 3.68WRR147 pKa = 11.84FGRR150 pKa = 11.84DD151 pKa = 2.88EE152 pKa = 4.08TEE154 pKa = 3.71YY155 pKa = 10.03TGRR158 pKa = 11.84SRR160 pKa = 11.84TEE162 pKa = 3.38FRR164 pKa = 11.84EE165 pKa = 3.8PDD167 pKa = 3.43TLTNWVNTNLLQVCIWTYY185 pKa = 10.35GPRR188 pKa = 11.84GGPQSPPLLSSPCLYY203 pKa = 10.12WLIWTQWARR212 pKa = 11.84SIDD215 pKa = 3.85STHH218 pKa = 6.41VYY220 pKa = 8.49CQGMFNLDD228 pKa = 3.14EE229 pKa = 4.66WNDD232 pKa = 3.32KK233 pKa = 10.65AKK235 pKa = 10.91YY236 pKa = 10.5VIFDD240 pKa = 5.36DD241 pKa = 4.71IDD243 pKa = 3.31IKK245 pKa = 11.17YY246 pKa = 9.25FPHH249 pKa = 6.34WKK251 pKa = 10.19SILGCQRR258 pKa = 11.84DD259 pKa = 3.49IQLTDD264 pKa = 2.92KK265 pKa = 10.61YY266 pKa = 10.35RR267 pKa = 11.84KK268 pKa = 9.41KK269 pKa = 10.33RR270 pKa = 11.84RR271 pKa = 11.84LRR273 pKa = 11.84NGLPCVWLCNEE284 pKa = 4.37DD285 pKa = 3.42MDD287 pKa = 5.28PRR289 pKa = 11.84GALSRR294 pKa = 11.84TQCEE298 pKa = 4.46WIEE301 pKa = 4.48SNCDD305 pKa = 3.11VVTLRR310 pKa = 11.84EE311 pKa = 4.1PLFEE315 pKa = 4.19

Molecular weight: 37.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075IZN1|A0A075IZN1_9VIRU Putative capsid protein OS=Sewage-associated circular DNA virus-3 OX=1519392 PE=4 SV=1
MM1 pKa = 7.58ASSLGQLAFGLAATAAVYY19 pKa = 9.99PLTRR23 pKa = 11.84EE24 pKa = 3.42VDD26 pKa = 3.46NYY28 pKa = 10.84VSNKK32 pKa = 9.65RR33 pKa = 11.84KK34 pKa = 10.35SEE36 pKa = 3.96DD37 pKa = 3.23SRR39 pKa = 11.84SFSVKK44 pKa = 9.87RR45 pKa = 11.84LKK47 pKa = 10.99LSTNKK52 pKa = 10.33DD53 pKa = 2.88MAFRR57 pKa = 11.84SRR59 pKa = 11.84RR60 pKa = 11.84SFRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84PRR67 pKa = 11.84RR68 pKa = 11.84FRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84FRR74 pKa = 11.84KK75 pKa = 9.14RR76 pKa = 11.84RR77 pKa = 11.84MPFRR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 11.84FTRR87 pKa = 11.84AVRR90 pKa = 11.84RR91 pKa = 11.84VILRR95 pKa = 11.84TSEE98 pKa = 4.18PKK100 pKa = 10.3QVWAPEE106 pKa = 4.0NVTGYY111 pKa = 10.9AIRR114 pKa = 11.84EE115 pKa = 3.92GDD117 pKa = 3.38GTSRR121 pKa = 11.84VINVSNPPAAPIQGVEE137 pKa = 3.87DD138 pKa = 3.73DD139 pKa = 3.9QYY141 pKa = 11.62IGNKK145 pKa = 9.72YY146 pKa = 8.75FLKK149 pKa = 10.86GIALRR154 pKa = 11.84GQVGTSGEE162 pKa = 4.11VTNRR166 pKa = 11.84QGCLIRR172 pKa = 11.84VTLVWSKK179 pKa = 10.51EE180 pKa = 3.71QAPNLFGAWTEE191 pKa = 4.22FTSATTSTAAPLQTPPDD208 pKa = 3.7LNPRR212 pKa = 11.84FFAITNGTQEE222 pKa = 4.02FVGNGWAIPFDD233 pKa = 3.61RR234 pKa = 11.84TRR236 pKa = 11.84VRR238 pKa = 11.84VISSKK243 pKa = 9.97TIVVNPGVDD252 pKa = 3.31NEE254 pKa = 4.38AGAGIISMPTAFSLYY269 pKa = 10.13FKK271 pKa = 10.18VNKK274 pKa = 8.7WMQIEE279 pKa = 5.17DD280 pKa = 3.78NDD282 pKa = 3.81QSDD285 pKa = 3.85MSSSLISFKK294 pKa = 11.11YY295 pKa = 7.53GTYY298 pKa = 9.32YY299 pKa = 11.35LVMQAVSNTNDD310 pKa = 3.15VTDD313 pKa = 3.55QTIAEE318 pKa = 4.23MDD320 pKa = 3.45YY321 pKa = 11.09SIGVFYY327 pKa = 10.72RR328 pKa = 11.84DD329 pKa = 3.36PP330 pKa = 4.65

Molecular weight: 37.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

645

315

330

322.5

37.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.426 ± 1.583

1.86 ± 1.147

5.891 ± 0.768

5.581 ± 1.21

5.581 ± 0.13

5.891 ± 0.348

1.24 ± 0.914

4.806 ± 0.254

3.876 ± 0.27

7.132 ± 1.459

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.86 ± 0.415

4.806 ± 0.478

5.271 ± 0.088

3.721 ± 0.161

10.078 ± 0.613

7.597 ± 0.654

6.977 ± 0.441

6.047 ± 1.35

2.481 ± 0.711

3.876 ± 0.4
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski