Amino acid dipepetide frequency for Microbacterium phage Efeko

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.554AlaAla: 22.554 ± 2.081
0.182AlaCys: 0.182 ± 0.158
8.185AlaAsp: 8.185 ± 1.111
8.003AlaGlu: 8.003 ± 1.244
3.82AlaPhe: 3.82 ± 0.627
15.46AlaGly: 15.46 ± 1.932
1.091AlaHis: 1.091 ± 0.482
4.911AlaIle: 4.911 ± 1.102
3.82AlaLys: 3.82 ± 1.242
13.459AlaLeu: 13.459 ± 1.535
3.274AlaMet: 3.274 ± 0.939
2.91AlaAsn: 2.91 ± 0.909
6.548AlaPro: 6.548 ± 1.084
2.546AlaGln: 2.546 ± 0.969
8.549AlaArg: 8.549 ± 1.565
6.366AlaSer: 6.366 ± 0.916
6.73AlaThr: 6.73 ± 1.044
8.003AlaVal: 8.003 ± 1.777
2.001AlaTrp: 2.001 ± 0.75
3.456AlaTyr: 3.456 ± 1.06
0.0AlaXaa: 0.0 ± 0.0
Cys
0.364CysAla: 0.364 ± 0.24
0.0CysCys: 0.0 ± 0.0
0.364CysAsp: 0.364 ± 0.264
0.728CysGlu: 0.728 ± 0.463
0.0CysPhe: 0.0 ± 0.0
0.546CysGly: 0.546 ± 0.675
0.0CysHis: 0.0 ± 0.0
0.0CysIle: 0.0 ± 0.0
0.0CysLys: 0.0 ± 0.0
0.364CysLeu: 0.364 ± 0.309
0.0CysMet: 0.0 ± 0.0
0.546CysAsn: 0.546 ± 0.339
0.364CysPro: 0.364 ± 0.411
0.0CysGln: 0.0 ± 0.0
0.546CysArg: 0.546 ± 0.451
0.0CysSer: 0.0 ± 0.0
0.546CysThr: 0.546 ± 0.381
0.182CysVal: 0.182 ± 0.206
0.182CysTrp: 0.182 ± 0.206
0.182CysTyr: 0.182 ± 0.189
0.0CysXaa: 0.0 ± 0.0
Asp
9.822AspAla: 9.822 ± 1.392
0.0AspCys: 0.0 ± 0.0
4.547AspAsp: 4.547 ± 1.402
2.91AspGlu: 2.91 ± 0.754
1.637AspPhe: 1.637 ± 0.529
4.365AspGly: 4.365 ± 1.231
1.273AspHis: 1.273 ± 0.569
0.728AspIle: 0.728 ± 0.314
0.728AspLys: 0.728 ± 0.42
5.457AspLeu: 5.457 ± 0.859
0.546AspMet: 0.546 ± 0.383
1.091AspAsn: 1.091 ± 0.538
3.638AspPro: 3.638 ± 0.716
2.546AspGln: 2.546 ± 0.535
2.183AspArg: 2.183 ± 0.716
2.364AspSer: 2.364 ± 0.615
3.82AspThr: 3.82 ± 0.932
6.184AspVal: 6.184 ± 1.458
0.909AspTrp: 0.909 ± 0.388
1.455AspTyr: 1.455 ± 0.529
0.0AspXaa: 0.0 ± 0.0
Glu
5.275GluAla: 5.275 ± 0.538
0.364GluCys: 0.364 ± 0.287
2.001GluAsp: 2.001 ± 0.476
1.091GluGlu: 1.091 ± 0.642
0.728GluPhe: 0.728 ± 0.33
4.001GluGly: 4.001 ± 0.773
1.091GluHis: 1.091 ± 0.596
3.456GluIle: 3.456 ± 0.898
0.364GluLys: 0.364 ± 0.204
9.64GluLeu: 9.64 ± 2.31
0.909GluMet: 0.909 ± 0.295
2.546GluAsn: 2.546 ± 0.813
2.364GluPro: 2.364 ± 0.625
3.274GluGln: 3.274 ± 0.764
4.365GluArg: 4.365 ± 1.259
2.91GluSer: 2.91 ± 0.512
5.093GluThr: 5.093 ± 0.921
2.364GluVal: 2.364 ± 0.614
0.909GluTrp: 0.909 ± 0.461
1.091GluTyr: 1.091 ± 0.456
0.0GluXaa: 0.0 ± 0.0
Phe
2.91PheAla: 2.91 ± 0.647
0.0PheCys: 0.0 ± 0.0
1.637PheAsp: 1.637 ± 0.496
1.273PheGlu: 1.273 ± 0.48
0.182PhePhe: 0.182 ± 0.2
4.001PheGly: 4.001 ± 0.836
0.0PheHis: 0.0 ± 0.0
0.909PheIle: 0.909 ± 0.382
0.546PheLys: 0.546 ± 0.225
2.001PheLeu: 2.001 ± 0.754
0.909PheMet: 0.909 ± 0.363
1.455PheAsn: 1.455 ± 0.644
1.091PhePro: 1.091 ± 0.688
1.637PheGln: 1.637 ± 0.518
1.273PheArg: 1.273 ± 0.555
2.183PheSer: 2.183 ± 0.737
3.456PheThr: 3.456 ± 0.854
2.001PheVal: 2.001 ± 0.464
0.546PheTrp: 0.546 ± 0.381
0.546PheTyr: 0.546 ± 0.329
0.0PheXaa: 0.0 ± 0.0
Gly
10.186GlyAla: 10.186 ± 1.532
0.546GlyCys: 0.546 ± 0.34
5.093GlyAsp: 5.093 ± 1.179
5.457GlyGlu: 5.457 ± 0.882
2.183GlyPhe: 2.183 ± 0.465
8.73GlyGly: 8.73 ± 1.633
1.273GlyHis: 1.273 ± 0.515
5.457GlyIle: 5.457 ± 0.828
3.274GlyLys: 3.274 ± 0.689
8.73GlyLeu: 8.73 ± 2.724
2.183GlyMet: 2.183 ± 0.497
2.364GlyAsn: 2.364 ± 0.652
2.728GlyPro: 2.728 ± 0.893
3.092GlyGln: 3.092 ± 0.775
6.912GlyArg: 6.912 ± 0.933
7.275GlySer: 7.275 ± 1.044
5.457GlyThr: 5.457 ± 0.783
7.093GlyVal: 7.093 ± 1.491
1.637GlyTrp: 1.637 ± 0.501
2.728GlyTyr: 2.728 ± 0.742
0.0GlyXaa: 0.0 ± 0.0
His
2.001HisAla: 2.001 ± 0.564
0.182HisCys: 0.182 ± 0.167
0.909HisAsp: 0.909 ± 0.479
0.182HisGlu: 0.182 ± 0.158
0.546HisPhe: 0.546 ± 0.274
1.273HisGly: 1.273 ± 0.509
0.546HisHis: 0.546 ± 0.396
0.182HisIle: 0.182 ± 0.206
0.182HisLys: 0.182 ± 0.189
1.819HisLeu: 1.819 ± 0.78
0.0HisMet: 0.0 ± 0.0
0.364HisAsn: 0.364 ± 0.379
1.091HisPro: 1.091 ± 0.579
0.546HisGln: 0.546 ± 0.245
0.728HisArg: 0.728 ± 0.357
0.364HisSer: 0.364 ± 0.204
0.728HisThr: 0.728 ± 0.386
1.455HisVal: 1.455 ± 0.628
0.0HisTrp: 0.0 ± 0.0
0.909HisTyr: 0.909 ± 0.346
0.0HisXaa: 0.0 ± 0.0
Ile
4.547IleAla: 4.547 ± 0.941
0.182IleCys: 0.182 ± 0.158
4.001IleAsp: 4.001 ± 0.806
5.275IleGlu: 5.275 ± 0.995
0.728IlePhe: 0.728 ± 0.328
3.092IleGly: 3.092 ± 0.565
0.728IleHis: 0.728 ± 0.293
2.183IleIle: 2.183 ± 1.034
0.364IleLys: 0.364 ± 0.245
2.728IleLeu: 2.728 ± 0.918
0.182IleMet: 0.182 ± 0.189
0.909IleAsn: 0.909 ± 0.456
2.183IlePro: 2.183 ± 0.629
0.909IleGln: 0.909 ± 0.29
4.365IleArg: 4.365 ± 1.008
4.001IleSer: 4.001 ± 0.83
2.91IleThr: 2.91 ± 0.761
4.547IleVal: 4.547 ± 1.23
0.364IleTrp: 0.364 ± 0.257
0.364IleTyr: 0.364 ± 0.411
0.0IleXaa: 0.0 ± 0.0
Lys
2.546LysAla: 2.546 ± 0.513
0.0LysCys: 0.0 ± 0.0
0.909LysAsp: 0.909 ± 0.609
1.091LysGlu: 1.091 ± 0.556
1.637LysPhe: 1.637 ± 0.6
1.091LysGly: 1.091 ± 0.398
0.546LysHis: 0.546 ± 0.324
0.728LysIle: 0.728 ± 0.383
0.182LysLys: 0.182 ± 0.203
2.001LysLeu: 2.001 ± 0.715
0.728LysMet: 0.728 ± 0.322
0.909LysAsn: 0.909 ± 0.473
1.637LysPro: 1.637 ± 0.636
0.909LysGln: 0.909 ± 0.394
2.546LysArg: 2.546 ± 0.951
1.091LysSer: 1.091 ± 0.42
1.637LysThr: 1.637 ± 0.563
0.909LysVal: 0.909 ± 0.529
0.182LysTrp: 0.182 ± 0.19
0.182LysTyr: 0.182 ± 0.158
0.0LysXaa: 0.0 ± 0.0
Leu
10.367LeuAla: 10.367 ± 0.803
0.364LeuCys: 0.364 ± 0.309
6.912LeuAsp: 6.912 ± 1.107
3.092LeuGlu: 3.092 ± 0.629
3.638LeuPhe: 3.638 ± 0.714
9.276LeuGly: 9.276 ± 1.319
1.637LeuHis: 1.637 ± 0.588
4.001LeuIle: 4.001 ± 1.112
0.909LeuLys: 0.909 ± 0.39
8.003LeuLeu: 8.003 ± 2.144
2.728LeuMet: 2.728 ± 0.583
3.456LeuAsn: 3.456 ± 0.911
6.548LeuPro: 6.548 ± 1.637
4.729LeuGln: 4.729 ± 0.757
6.548LeuArg: 6.548 ± 1.334
5.093LeuSer: 5.093 ± 0.956
7.639LeuThr: 7.639 ± 1.104
8.003LeuVal: 8.003 ± 1.249
0.546LeuTrp: 0.546 ± 0.282
1.455LeuTyr: 1.455 ± 0.519
0.0LeuXaa: 0.0 ± 0.0
Met
2.364MetAla: 2.364 ± 0.608
0.0MetCys: 0.0 ± 0.0
0.546MetAsp: 0.546 ± 0.36
0.182MetGlu: 0.182 ± 0.157
1.273MetPhe: 1.273 ± 0.498
2.546MetGly: 2.546 ± 0.865
0.364MetHis: 0.364 ± 0.263
0.728MetIle: 0.728 ± 0.383
0.182MetLys: 0.182 ± 0.206
1.637MetLeu: 1.637 ± 0.541
0.0MetMet: 0.0 ± 0.0
1.091MetAsn: 1.091 ± 0.419
1.455MetPro: 1.455 ± 0.494
0.182MetGln: 0.182 ± 0.157
1.091MetArg: 1.091 ± 0.542
2.91MetSer: 2.91 ± 0.761
2.91MetThr: 2.91 ± 0.725
1.637MetVal: 1.637 ± 0.449
0.182MetTrp: 0.182 ± 0.206
0.182MetTyr: 0.182 ± 0.158
0.0MetXaa: 0.0 ± 0.0
Asn
4.729AsnAla: 4.729 ± 0.941
0.182AsnCys: 0.182 ± 0.206
1.273AsnAsp: 1.273 ± 0.408
0.728AsnGlu: 0.728 ± 0.394
0.546AsnPhe: 0.546 ± 0.311
3.274AsnGly: 3.274 ± 0.644
0.364AsnHis: 0.364 ± 0.249
0.909AsnIle: 0.909 ± 0.285
0.546AsnLys: 0.546 ± 0.313
2.91AsnLeu: 2.91 ± 0.568
1.091AsnMet: 1.091 ± 0.377
0.364AsnAsn: 0.364 ± 0.267
2.001AsnPro: 2.001 ± 0.579
0.546AsnGln: 0.546 ± 0.305
2.546AsnArg: 2.546 ± 0.474
2.183AsnSer: 2.183 ± 0.632
2.001AsnThr: 2.001 ± 0.74
2.001AsnVal: 2.001 ± 0.381
0.0AsnTrp: 0.0 ± 0.0
0.546AsnTyr: 0.546 ± 0.434
0.0AsnXaa: 0.0 ± 0.0
Pro
8.367ProAla: 8.367 ± 1.223
0.546ProCys: 0.546 ± 0.394
2.91ProAsp: 2.91 ± 0.761
2.183ProGlu: 2.183 ± 0.87
1.273ProPhe: 1.273 ± 0.48
3.274ProGly: 3.274 ± 0.567
0.728ProHis: 0.728 ± 0.505
2.183ProIle: 2.183 ± 0.608
2.001ProLys: 2.001 ± 0.553
3.82ProLeu: 3.82 ± 0.582
1.273ProMet: 1.273 ± 0.461
1.273ProAsn: 1.273 ± 0.472
2.91ProPro: 2.91 ± 1.153
2.91ProGln: 2.91 ± 1.385
2.546ProArg: 2.546 ± 0.806
2.728ProSer: 2.728 ± 0.617
3.456ProThr: 3.456 ± 0.978
4.183ProVal: 4.183 ± 0.73
0.546ProTrp: 0.546 ± 0.264
1.455ProTyr: 1.455 ± 0.49
0.0ProXaa: 0.0 ± 0.0
Gln
2.183GlnAla: 2.183 ± 0.608
0.0GlnCys: 0.0 ± 0.0
1.819GlnAsp: 1.819 ± 0.459
2.364GlnGlu: 2.364 ± 0.583
1.819GlnPhe: 1.819 ± 0.42
2.91GlnGly: 2.91 ± 0.93
0.546GlnHis: 0.546 ± 0.306
3.092GlnIle: 3.092 ± 0.76
0.909GlnLys: 0.909 ± 0.58
7.821GlnLeu: 7.821 ± 1.25
1.091GlnMet: 1.091 ± 0.458
1.637GlnAsn: 1.637 ± 0.492
1.455GlnPro: 1.455 ± 0.415
2.183GlnGln: 2.183 ± 0.65
3.092GlnArg: 3.092 ± 0.703
2.001GlnSer: 2.001 ± 0.901
2.183GlnThr: 2.183 ± 0.455
0.728GlnVal: 0.728 ± 0.322
0.546GlnTrp: 0.546 ± 0.315
1.273GlnTyr: 1.273 ± 0.366
0.0GlnXaa: 0.0 ± 0.0
Arg
8.912ArgAla: 8.912 ± 1.935
0.182ArgCys: 0.182 ± 0.206
3.638ArgAsp: 3.638 ± 0.799
5.093ArgGlu: 5.093 ± 1.039
2.728ArgPhe: 2.728 ± 0.95
5.093ArgGly: 5.093 ± 1.149
1.091ArgHis: 1.091 ± 0.403
3.456ArgIle: 3.456 ± 0.694
1.455ArgLys: 1.455 ± 0.423
6.184ArgLeu: 6.184 ± 1.188
1.273ArgMet: 1.273 ± 0.52
0.909ArgAsn: 0.909 ± 0.439
1.819ArgPro: 1.819 ± 0.481
2.91ArgGln: 2.91 ± 0.688
9.094ArgArg: 9.094 ± 2.156
4.547ArgSer: 4.547 ± 0.904
3.274ArgThr: 3.274 ± 1.168
4.729ArgVal: 4.729 ± 0.742
2.728ArgTrp: 2.728 ± 0.768
1.819ArgTyr: 1.819 ± 0.5
0.0ArgXaa: 0.0 ± 0.0
Ser
10.549SerAla: 10.549 ± 1.193
0.364SerCys: 0.364 ± 0.415
1.819SerAsp: 1.819 ± 0.558
2.546SerGlu: 2.546 ± 0.479
0.546SerPhe: 0.546 ± 0.25
6.002SerGly: 6.002 ± 1.256
1.091SerHis: 1.091 ± 0.369
3.82SerIle: 3.82 ± 0.89
2.183SerLys: 2.183 ± 0.666
3.274SerLeu: 3.274 ± 0.933
1.091SerMet: 1.091 ± 0.454
1.455SerAsn: 1.455 ± 0.397
2.728SerPro: 2.728 ± 0.666
3.092SerGln: 3.092 ± 0.796
3.092SerArg: 3.092 ± 0.653
5.275SerSer: 5.275 ± 1.246
4.183SerThr: 4.183 ± 0.828
6.366SerVal: 6.366 ± 1.106
1.091SerTrp: 1.091 ± 0.387
1.637SerTyr: 1.637 ± 0.402
0.0SerXaa: 0.0 ± 0.0
Thr
9.458ThrAla: 9.458 ± 1.783
0.546ThrCys: 0.546 ± 0.381
3.82ThrAsp: 3.82 ± 0.839
3.456ThrGlu: 3.456 ± 0.991
3.274ThrPhe: 3.274 ± 0.988
9.094ThrGly: 9.094 ± 1.655
0.182ThrHis: 0.182 ± 0.167
4.001ThrIle: 4.001 ± 1.262
1.273ThrLys: 1.273 ± 0.547
6.366ThrLeu: 6.366 ± 0.657
1.819ThrMet: 1.819 ± 0.712
2.183ThrAsn: 2.183 ± 0.503
4.183ThrPro: 4.183 ± 0.88
1.091ThrGln: 1.091 ± 0.341
3.638ThrArg: 3.638 ± 1.02
3.456ThrSer: 3.456 ± 0.827
3.274ThrThr: 3.274 ± 0.762
6.366ThrVal: 6.366 ± 1.224
0.728ThrTrp: 0.728 ± 0.352
1.273ThrTyr: 1.273 ± 0.398
0.0ThrXaa: 0.0 ± 0.0
Val
8.73ValAla: 8.73 ± 1.129
0.546ValCys: 0.546 ± 0.48
3.82ValAsp: 3.82 ± 0.809
5.82ValGlu: 5.82 ± 1.292
0.909ValPhe: 0.909 ± 0.424
5.82ValGly: 5.82 ± 1.217
0.728ValHis: 0.728 ± 0.348
1.637ValIle: 1.637 ± 0.6
2.183ValLys: 2.183 ± 0.707
6.002ValLeu: 6.002 ± 0.967
1.819ValMet: 1.819 ± 0.706
2.183ValAsn: 2.183 ± 0.639
3.82ValPro: 3.82 ± 0.859
4.911ValGln: 4.911 ± 1.083
5.275ValArg: 5.275 ± 1.021
5.638ValSer: 5.638 ± 1.167
7.457ValThr: 7.457 ± 1.285
6.912ValVal: 6.912 ± 0.874
0.909ValTrp: 0.909 ± 0.377
1.637ValTyr: 1.637 ± 0.566
0.0ValXaa: 0.0 ± 0.0
Trp
2.001TrpAla: 2.001 ± 0.708
0.182TrpCys: 0.182 ± 0.206
0.546TrpAsp: 0.546 ± 0.372
0.909TrpGlu: 0.909 ± 0.582
0.728TrpPhe: 0.728 ± 0.379
0.182TrpGly: 0.182 ± 0.183
0.546TrpHis: 0.546 ± 0.445
0.909TrpIle: 0.909 ± 0.302
0.182TrpLys: 0.182 ± 0.158
2.364TrpLeu: 2.364 ± 0.684
0.182TrpMet: 0.182 ± 0.189
0.364TrpAsn: 0.364 ± 0.226
0.364TrpPro: 0.364 ± 0.296
0.546TrpGln: 0.546 ± 0.298
0.909TrpArg: 0.909 ± 0.463
0.909TrpSer: 0.909 ± 0.484
1.455TrpThr: 1.455 ± 0.515
0.546TrpVal: 0.546 ± 0.246
0.0TrpTrp: 0.0 ± 0.0
0.364TrpTyr: 0.364 ± 0.206
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.001TyrAla: 4.001 ± 0.674
0.546TyrCys: 0.546 ± 0.326
1.455TyrAsp: 1.455 ± 0.467
1.455TyrGlu: 1.455 ± 0.345
0.364TyrPhe: 0.364 ± 0.229
2.364TyrGly: 2.364 ± 0.671
0.182TyrHis: 0.182 ± 0.158
1.273TyrIle: 1.273 ± 0.552
0.364TyrLys: 0.364 ± 0.309
0.364TyrLeu: 0.364 ± 0.257
0.182TyrMet: 0.182 ± 0.189
0.728TyrAsn: 0.728 ± 0.31
1.637TyrPro: 1.637 ± 0.45
1.091TyrGln: 1.091 ± 0.445
1.637TyrArg: 1.637 ± 0.598
0.909TyrSer: 0.909 ± 0.381
1.273TyrThr: 1.273 ± 0.4
2.546TyrVal: 2.546 ± 0.752
0.182TyrTrp: 0.182 ± 0.158
0.728TyrTyr: 0.728 ± 0.426
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 28 proteins (5499 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski