Amino acid dipepetide frequency for Clostridium sp. CAG:43

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.622AlaAla: 9.622 ± 0.171	1.251AlaCys: 1.251 ± 0.045	4.955AlaAsp: 4.955 ± 0.099	6.306AlaGlu: 6.306 ± 0.111	3.252AlaPhe: 3.252 ± 0.081	7.379AlaGly: 7.379 ± 0.131	1.215AlaHis: 1.215 ± 0.049	5.127AlaIle: 5.127 ± 0.093	4.768AlaLys: 4.768 ± 0.091	7.625AlaLeu: 7.625 ± 0.113	2.615AlaMet: 2.615 ± 0.064	2.411AlaAsn: 2.411 ± 0.06	2.304AlaPro: 2.304 ± 0.061	2.643AlaGln: 2.643 ± 0.066	3.535AlaArg: 3.535 ± 0.076	4.622AlaSer: 4.622 ± 0.09	3.102AlaThr: 3.102 ± 0.075	6.759AlaVal: 6.759 ± 0.125	0.737AlaTrp: 0.737 ± 0.04	2.864AlaTyr: 2.864 ± 0.069	0.026AlaXaa: 0.026 ± 0.005
Cys	1.03CysAla: 1.03 ± 0.037	0.333CysCys: 0.333 ± 0.028	0.765CysAsp: 0.765 ± 0.037	0.923CysGlu: 0.923 ± 0.038	0.69CysPhe: 0.69 ± 0.031	1.544CysGly: 1.544 ± 0.058	0.385CysHis: 0.385 ± 0.026	0.984CysIle: 0.984 ± 0.039	0.662CysLys: 0.662 ± 0.03	1.31CysLeu: 1.31 ± 0.045	0.408CysMet: 0.408 ± 0.026	0.434CysAsn: 0.434 ± 0.026	0.665CysPro: 0.665 ± 0.037	0.508CysGln: 0.508 ± 0.029	0.918CysArg: 0.918 ± 0.038	0.926CysSer: 0.926 ± 0.037	0.721CysThr: 0.721 ± 0.032	1.062CysVal: 1.062 ± 0.046	0.12CysTrp: 0.12 ± 0.013	0.512CysTyr: 0.512 ± 0.03	0.001CysXaa: 0.001 ± 0.001
Asp	4.599AspAla: 4.599 ± 0.086	0.746AspCys: 0.746 ± 0.029	2.703AspAsp: 2.703 ± 0.087	4.555AspGlu: 4.555 ± 0.081	2.274AspPhe: 2.274 ± 0.065	5.02AspGly: 5.02 ± 0.117	0.974AspHis: 0.974 ± 0.038	3.63AspIle: 3.63 ± 0.077	3.108AspLys: 3.108 ± 0.087	4.626AspLeu: 4.626 ± 0.085	1.73AspMet: 1.73 ± 0.048	1.779AspAsn: 1.779 ± 0.054	2.037AspPro: 2.037 ± 0.067	1.361AspGln: 1.361 ± 0.049	2.632AspArg: 2.632 ± 0.061	2.968AspSer: 2.968 ± 0.071	2.85AspThr: 2.85 ± 0.07	3.649AspVal: 3.649 ± 0.079	0.576AspTrp: 0.576 ± 0.031	2.358AspTyr: 2.358 ± 0.056	0.001AspXaa: 0.001 ± 0.001
Glu	6.795GluAla: 6.795 ± 0.13	0.841GluCys: 0.841 ± 0.039	4.107GluAsp: 4.107 ± 0.089	7.447GluGlu: 7.447 ± 0.15	2.424GluPhe: 2.424 ± 0.064	4.286GluGly: 4.286 ± 0.081	1.424GluHis: 1.424 ± 0.05	5.226GluIle: 5.226 ± 0.1	6.788GluLys: 6.788 ± 0.133	6.773GluLeu: 6.773 ± 0.119	2.665GluMet: 2.665 ± 0.059	3.954GluAsn: 3.954 ± 0.076	2.12GluPro: 2.12 ± 0.056	2.905GluGln: 2.905 ± 0.067	3.931GluArg: 3.931 ± 0.082	3.557GluSer: 3.557 ± 0.089	4.291GluThr: 4.291 ± 0.091	4.768GluVal: 4.768 ± 0.084	0.696GluTrp: 0.696 ± 0.034	2.921GluTyr: 2.921 ± 0.078	0.001GluXaa: 0.001 ± 0.001
Phe	3.01PheAla: 3.01 ± 0.073	0.76PheCys: 0.76 ± 0.038	2.284PheAsp: 2.284 ± 0.057	2.529PheGlu: 2.529 ± 0.055	1.737PhePhe: 1.737 ± 0.074	3.159PheGly: 3.159 ± 0.085	0.879PheHis: 0.879 ± 0.04	2.587PheIle: 2.587 ± 0.075	1.776PheLys: 1.776 ± 0.046	4.032PheLeu: 4.032 ± 0.106	1.151PheMet: 1.151 ± 0.043	1.368PheAsn: 1.368 ± 0.044	1.459PhePro: 1.459 ± 0.045	1.237PheGln: 1.237 ± 0.044	1.711PheArg: 1.711 ± 0.054	2.763PheSer: 2.763 ± 0.079	2.153PheThr: 2.153 ± 0.062	2.69PheVal: 2.69 ± 0.073	0.427PheTrp: 0.427 ± 0.024	1.662PheTyr: 1.662 ± 0.052	0.001PheXaa: 0.001 ± 0.001
Gly	5.925GlyAla: 5.925 ± 0.12	1.351GlyCys: 1.351 ± 0.05	3.61GlyAsp: 3.61 ± 0.08	5.242GlyGlu: 5.242 ± 0.094	3.156GlyPhe: 3.156 ± 0.066	5.655GlyGly: 5.655 ± 0.106	1.41GlyHis: 1.41 ± 0.045	6.068GlyIle: 6.068 ± 0.092	5.352GlyLys: 5.352 ± 0.085	6.164GlyLeu: 6.164 ± 0.106	2.602GlyMet: 2.602 ± 0.069	3.05GlyAsn: 3.05 ± 0.068	1.56GlyPro: 1.56 ± 0.051	2.314GlyGln: 2.314 ± 0.053	3.768GlyArg: 3.768 ± 0.08	4.54GlySer: 4.54 ± 0.082	4.791GlyThr: 4.791 ± 0.097	5.447GlyVal: 5.447 ± 0.092	0.885GlyTrp: 0.885 ± 0.039	3.275GlyTyr: 3.275 ± 0.07	0.007GlyXaa: 0.007 ± 0.003
His	1.231HisAla: 1.231 ± 0.041	0.299HisCys: 0.299 ± 0.019	0.922HisAsp: 0.922 ± 0.045	1.117HisGlu: 1.117 ± 0.038	0.889HisPhe: 0.889 ± 0.037	1.414HisGly: 1.414 ± 0.053	0.476HisHis: 0.476 ± 0.035	1.316HisIle: 1.316 ± 0.039	0.876HisLys: 0.876 ± 0.034	1.713HisLeu: 1.713 ± 0.048	0.573HisMet: 0.573 ± 0.028	0.632HisAsn: 0.632 ± 0.029	1.04HisPro: 1.04 ± 0.039	0.532HisGln: 0.532 ± 0.027	0.899HisArg: 0.899 ± 0.036	1.068HisSer: 1.068 ± 0.036	1.03HisThr: 1.03 ± 0.046	1.234HisVal: 1.234 ± 0.048	0.183HisTrp: 0.183 ± 0.016	0.736HisTyr: 0.736 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.525IleAla: 5.525 ± 0.099	1.268IleCys: 1.268 ± 0.04	3.717IleAsp: 3.717 ± 0.09	4.606IleGlu: 4.606 ± 0.081	2.563IlePhe: 2.563 ± 0.076	5.278IleGly: 5.278 ± 0.104	1.369IleHis: 1.369 ± 0.047	4.079IleIle: 4.079 ± 0.106	3.218IleLys: 3.218 ± 0.082	6.863IleLeu: 6.863 ± 0.132	1.592IleMet: 1.592 ± 0.058	2.414IleAsn: 2.414 ± 0.062	3.15IlePro: 3.15 ± 0.074	2.333IleGln: 2.333 ± 0.064	3.968IleArg: 3.968 ± 0.075	4.2IleSer: 4.2 ± 0.087	3.407IleThr: 3.407 ± 0.068	4.747IleVal: 4.747 ± 0.101	0.623IleTrp: 0.623 ± 0.029	2.391IleTyr: 2.391 ± 0.063	0.001IleXaa: 0.001 ± 0.002
Lys	4.899LysAla: 4.899 ± 0.099	0.662LysCys: 0.662 ± 0.033	3.26LysAsp: 3.26 ± 0.08	6.101LysGlu: 6.101 ± 0.118	1.701LysPhe: 1.701 ± 0.055	3.831LysGly: 3.831 ± 0.066	0.936LysHis: 0.936 ± 0.036	4.097LysIle: 4.097 ± 0.081	5.603LysLys: 5.603 ± 0.095	5.089LysLeu: 5.089 ± 0.091	2.28LysMet: 2.28 ± 0.065	3.091LysAsn: 3.091 ± 0.065	2.11LysPro: 2.11 ± 0.056	2.189LysGln: 2.189 ± 0.055	3.235LysArg: 3.235 ± 0.079	2.857LysSer: 2.857 ± 0.067	3.516LysThr: 3.516 ± 0.078	3.915LysVal: 3.915 ± 0.073	0.612LysTrp: 0.612 ± 0.031	2.392LysTyr: 2.392 ± 0.061	0.004LysXaa: 0.004 ± 0.003
Leu	7.746LeuAla: 7.746 ± 0.117	1.478LeuCys: 1.478 ± 0.047	5.101LeuAsp: 5.101 ± 0.103	6.513LeuGlu: 6.513 ± 0.121	3.762LeuPhe: 3.762 ± 0.099	6.379LeuGly: 6.379 ± 0.093	1.613LeuHis: 1.613 ± 0.044	5.824LeuIle: 5.824 ± 0.104	5.636LeuLys: 5.636 ± 0.106	8.633LeuLeu: 8.633 ± 0.156	2.732LeuMet: 2.732 ± 0.071	3.532LeuAsn: 3.532 ± 0.073	3.786LeuPro: 3.786 ± 0.083	2.827LeuGln: 2.827 ± 0.064	4.436LeuArg: 4.436 ± 0.097	6.068LeuSer: 6.068 ± 0.119	5.118LeuThr: 5.118 ± 0.084	5.599LeuVal: 5.599 ± 0.108	0.76LeuTrp: 0.76 ± 0.038	3.329LeuTyr: 3.329 ± 0.074	0.01LeuXaa: 0.01 ± 0.004
Met	2.844MetAla: 2.844 ± 0.072	0.342MetCys: 0.342 ± 0.024	1.944MetAsp: 1.944 ± 0.063	2.648MetGlu: 2.648 ± 0.061	1.037MetPhe: 1.037 ± 0.046	2.302MetGly: 2.302 ± 0.08	0.476MetHis: 0.476 ± 0.025	2.079MetIle: 2.079 ± 0.059	2.326MetLys: 2.326 ± 0.055	2.687MetLeu: 2.687 ± 0.065	1.049MetMet: 1.049 ± 0.04	1.436MetAsn: 1.436 ± 0.045	1.231MetPro: 1.231 ± 0.041	1.173MetGln: 1.173 ± 0.042	1.511MetArg: 1.511 ± 0.047	1.629MetSer: 1.629 ± 0.055	1.85MetThr: 1.85 ± 0.045	2.055MetVal: 2.055 ± 0.055	0.212MetTrp: 0.212 ± 0.019	0.84MetTyr: 0.84 ± 0.039	0.0MetXaa: 0.0 ± 0.0
Asn	3.023AsnAla: 3.023 ± 0.073	0.597AsnCys: 0.597 ± 0.029	1.828AsnAsp: 1.828 ± 0.055	2.561AsnGlu: 2.561 ± 0.063	1.384AsnPhe: 1.384 ± 0.048	3.31AsnGly: 3.31 ± 0.082	0.804AsnHis: 0.804 ± 0.034	2.571AsnIle: 2.571 ± 0.064	1.99AsnLys: 1.99 ± 0.067	3.597AsnLeu: 3.597 ± 0.071	1.173AsnMet: 1.173 ± 0.045	1.341AsnAsn: 1.341 ± 0.05	1.952AsnPro: 1.952 ± 0.058	1.502AsnGln: 1.502 ± 0.046	2.089AsnArg: 2.089 ± 0.051	1.98AsnSer: 1.98 ± 0.059	2.058AsnThr: 2.058 ± 0.056	2.661AsnVal: 2.661 ± 0.063	0.362AsnTrp: 0.362 ± 0.024	1.517AsnTyr: 1.517 ± 0.05	0.0AsnXaa: 0.0 ± 0.0
Pro	2.84ProAla: 2.84 ± 0.072	0.518ProCys: 0.518 ± 0.025	2.457ProAsp: 2.457 ± 0.063	3.918ProGlu: 3.918 ± 0.084	1.619ProPhe: 1.619 ± 0.054	2.75ProGly: 2.75 ± 0.07	0.632ProHis: 0.632 ± 0.028	2.189ProIle: 2.189 ± 0.061	1.749ProLys: 1.749 ± 0.049	2.965ProLeu: 2.965 ± 0.068	1.071ProMet: 1.071 ± 0.041	1.167ProAsn: 1.167 ± 0.048	0.818ProPro: 0.818 ± 0.035	1.084ProGln: 1.084 ± 0.04	1.185ProArg: 1.185 ± 0.046	1.919ProSer: 1.919 ± 0.051	1.486ProThr: 1.486 ± 0.045	3.136ProVal: 3.136 ± 0.068	0.339ProTrp: 0.339 ± 0.025	1.433ProTyr: 1.433 ± 0.048	0.003ProXaa: 0.003 ± 0.002
Gln	2.857GlnAla: 2.857 ± 0.056	0.358GlnCys: 0.358 ± 0.021	1.535GlnAsp: 1.535 ± 0.047	2.83GlnGlu: 2.83 ± 0.085	1.265GlnPhe: 1.265 ± 0.045	2.169GlnGly: 2.169 ± 0.059	0.457GlnHis: 0.457 ± 0.022	2.603GlnIle: 2.603 ± 0.053	2.59GlnLys: 2.59 ± 0.064	2.743GlnLeu: 2.743 ± 0.066	1.43GlnMet: 1.43 ± 0.049	1.538GlnAsn: 1.538 ± 0.042	1.026GlnPro: 1.026 ± 0.042	1.069GlnGln: 1.069 ± 0.045	1.358GlnArg: 1.358 ± 0.045	1.586GlnSer: 1.586 ± 0.044	1.986GlnThr: 1.986 ± 0.062	2.237GlnVal: 2.237 ± 0.065	0.296GlnTrp: 0.296 ± 0.02	1.302GlnTyr: 1.302 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	3.423ArgAla: 3.423 ± 0.069	0.68ArgCys: 0.68 ± 0.029	2.502ArgAsp: 2.502 ± 0.062	4.717ArgGlu: 4.717 ± 0.091	1.957ArgPhe: 1.957 ± 0.056	3.115ArgGly: 3.115 ± 0.064	0.918ArgHis: 0.918 ± 0.038	3.603ArgIle: 3.603 ± 0.071	3.452ArgLys: 3.452 ± 0.073	4.577ArgLeu: 4.577 ± 0.106	1.756ArgMet: 1.756 ± 0.049	1.789ArgAsn: 1.789 ± 0.052	1.566ArgPro: 1.566 ± 0.05	2.003ArgGln: 2.003 ± 0.058	3.068ArgArg: 3.068 ± 0.092	2.482ArgSer: 2.482 ± 0.057	2.537ArgThr: 2.537 ± 0.063	3.104ArgVal: 3.104 ± 0.064	0.431ArgTrp: 0.431 ± 0.025	1.919ArgTyr: 1.919 ± 0.06	0.004ArgXaa: 0.004 ± 0.003
Ser	4.46SerAla: 4.46 ± 0.099	0.824SerCys: 0.824 ± 0.036	3.078SerAsp: 3.078 ± 0.085	3.971SerGlu: 3.971 ± 0.09	2.518SerPhe: 2.518 ± 0.064	5.477SerGly: 5.477 ± 0.103	1.026SerHis: 1.026 ± 0.04	3.782SerIle: 3.782 ± 0.09	2.721SerLys: 2.721 ± 0.064	5.128SerLeu: 5.128 ± 0.1	1.74SerMet: 1.74 ± 0.049	1.975SerAsn: 1.975 ± 0.062	1.814SerPro: 1.814 ± 0.052	1.951SerGln: 1.951 ± 0.055	3.065SerArg: 3.065 ± 0.07	3.525SerSer: 3.525 ± 0.123	2.577SerThr: 2.577 ± 0.059	4.161SerVal: 4.161 ± 0.078	0.618SerTrp: 0.618 ± 0.03	2.358SerTyr: 2.358 ± 0.063	0.003SerXaa: 0.003 ± 0.002
Thr	4.897ThrAla: 4.897 ± 0.092	0.69ThrCys: 0.69 ± 0.034	2.889ThrAsp: 2.889 ± 0.065	3.815ThrGlu: 3.815 ± 0.087	1.981ThrPhe: 1.981 ± 0.048	4.873ThrGly: 4.873 ± 0.099	0.921ThrHis: 0.921 ± 0.038	3.661ThrIle: 3.661 ± 0.092	2.799ThrLys: 2.799 ± 0.064	4.62ThrLeu: 4.62 ± 0.077	1.505ThrMet: 1.505 ± 0.049	1.804ThrAsn: 1.804 ± 0.055	2.267ThrPro: 2.267 ± 0.069	1.405ThrGln: 1.405 ± 0.045	2.108ThrArg: 2.108 ± 0.059	2.899ThrSer: 2.899 ± 0.077	2.668ThrThr: 2.668 ± 0.074	4.483ThrVal: 4.483 ± 0.09	0.527ThrTrp: 0.527 ± 0.03	1.931ThrTyr: 1.931 ± 0.059	0.001ThrXaa: 0.001 ± 0.001
Val	5.006ValAla: 5.006 ± 0.097	1.235ValCys: 1.235 ± 0.043	3.824ValAsp: 3.824 ± 0.091	4.665ValGlu: 4.665 ± 0.092	3.001ValPhe: 3.001 ± 0.082	4.643ValGly: 4.643 ± 0.092	1.235ValHis: 1.235 ± 0.04	4.83ValIle: 4.83 ± 0.09	4.258ValLys: 4.258 ± 0.093	6.89ValLeu: 6.89 ± 0.12	2.12ValMet: 2.12 ± 0.061	2.596ValAsn: 2.596 ± 0.061	2.729ValPro: 2.729 ± 0.069	2.218ValGln: 2.218 ± 0.059	3.502ValArg: 3.502 ± 0.078	4.587ValSer: 4.587 ± 0.082	4.066ValThr: 4.066 ± 0.089	4.838ValVal: 4.838 ± 0.103	0.649ValTrp: 0.649 ± 0.037	2.678ValTyr: 2.678 ± 0.07	0.003ValXaa: 0.003 ± 0.002
Trp	0.564TrpAla: 0.564 ± 0.031	0.159TrpCys: 0.159 ± 0.016	0.457TrpAsp: 0.457 ± 0.027	0.681TrpGlu: 0.681 ± 0.031	0.417TrpPhe: 0.417 ± 0.027	0.672TrpGly: 0.672 ± 0.029	0.188TrpHis: 0.188 ± 0.018	0.733TrpIle: 0.733 ± 0.035	0.786TrpLys: 0.786 ± 0.033	0.964TrpLeu: 0.964 ± 0.045	0.411TrpMet: 0.411 ± 0.024	0.506TrpAsn: 0.506 ± 0.027	0.172TrpPro: 0.172 ± 0.018	0.43TrpGln: 0.43 ± 0.027	0.436TrpArg: 0.436 ± 0.027	0.46TrpSer: 0.46 ± 0.024	0.413TrpThr: 0.413 ± 0.026	0.493TrpVal: 0.493 ± 0.024	0.144TrpTrp: 0.144 ± 0.016	0.465TrpTyr: 0.465 ± 0.029	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.82TyrAla: 2.82 ± 0.066	0.505TyrCys: 0.505 ± 0.029	2.238TyrAsp: 2.238 ± 0.059	2.769TyrGlu: 2.769 ± 0.064	1.746TyrPhe: 1.746 ± 0.046	2.906TyrGly: 2.906 ± 0.059	0.84TyrHis: 0.84 ± 0.035	2.325TyrIle: 2.325 ± 0.057	1.919TyrLys: 1.919 ± 0.059	3.871TyrLeu: 3.871 ± 0.089	1.065TyrMet: 1.065 ± 0.038	1.48TyrAsn: 1.48 ± 0.052	1.463TyrPro: 1.463 ± 0.044	1.547TyrGln: 1.547 ± 0.053	2.244TyrArg: 2.244 ± 0.062	2.136TyrSer: 2.136 ± 0.052	2.121TyrThr: 2.121 ± 0.053	2.535TyrVal: 2.535 ± 0.06	0.354TyrTrp: 0.354 ± 0.022	1.818TyrTyr: 1.818 ± 0.059	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.004XaaAla: 0.004 ± 0.002	0.007XaaCys: 0.007 ± 0.003	0.001XaaAsp: 0.001 ± 0.002	0.003XaaGlu: 0.003 ± 0.002	0.003XaaPhe: 0.003 ± 0.002	0.007XaaGly: 0.007 ± 0.003	0.001XaaHis: 0.001 ± 0.002	0.0XaaIle: 0.0 ± 0.0	0.003XaaLys: 0.003 ± 0.002	0.01XaaLeu: 0.01 ± 0.004	0.001XaaMet: 0.001 ± 0.002	0.001XaaAsn: 0.001 ± 0.002	0.009XaaPro: 0.009 ± 0.003	0.003XaaGln: 0.003 ± 0.002	0.004XaaArg: 0.004 ± 0.002	0.003XaaSer: 0.003 ± 0.002	0.003XaaThr: 0.003 ± 0.002	0.001XaaVal: 0.001 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.001XaaTyr: 0.001 ± 0.001	0.084XaaXaa: 0.084 ± 0.014

Statistics based on 2273 proteins (693025 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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