candidate division MSBL1 archaeon SCGC-AAA259E19

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1417 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133UJT4|A0A133UJT4_9EURY Gluconolaconase OS=candidate division MSBL1 archaeon SCGC-AAA259E19 OX=1698264 GN=AKJ65_04335 PE=4 SV=1
MM1 pKa = 7.29FVTSNILLFDD11 pKa = 3.95SVGGQVWDD19 pKa = 4.56DD20 pKa = 3.88NEE22 pKa = 4.44EE23 pKa = 4.22PTSDD27 pKa = 3.71STPKK31 pKa = 10.16ISLTISDD38 pKa = 3.81VGLGVALEE46 pKa = 4.45DD47 pKa = 4.67LTVQLDD53 pKa = 3.71NNDD56 pKa = 3.44NFEE59 pKa = 5.33DD60 pKa = 3.94GTPYY64 pKa = 10.48TGISPLEE71 pKa = 4.08NSNDD75 pKa = 3.73YY76 pKa = 9.07TWSVEE81 pKa = 3.66NGAIFEE87 pKa = 4.27NTIEE91 pKa = 4.08NAGVGLHH98 pKa = 7.09DD99 pKa = 3.77GTYY102 pKa = 10.59ACGKK106 pKa = 9.9RR107 pKa = 11.84AFF109 pKa = 4.03

Molecular weight: 11.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133UNP8|A0A133UNP8_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA259E19 OX=1698264 GN=AKJ65_00560 PE=4 SV=1
MM1 pKa = 7.4SRR3 pKa = 11.84NKK5 pKa = 10.47SLATKK10 pKa = 10.24KK11 pKa = 10.52KK12 pKa = 9.08LAKK15 pKa = 10.03AGKK18 pKa = 6.95QRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84VPVWVMAKK30 pKa = 8.98TGGRR34 pKa = 11.84VRR36 pKa = 11.84THH38 pKa = 6.09RR39 pKa = 11.84KK40 pKa = 7.26SRR42 pKa = 11.84HH43 pKa = 3.66WRR45 pKa = 11.84RR46 pKa = 11.84QNIKK50 pKa = 9.78PP51 pKa = 3.58

Molecular weight: 6.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1417

0

1417

311721

44

1451

220.0

24.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.965 ± 0.063

0.987 ± 0.024

5.788 ± 0.063

10.437 ± 0.095

3.933 ± 0.046

7.651 ± 0.07

1.703 ± 0.03

6.53 ± 0.056

7.22 ± 0.077

9.171 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.227 ± 0.03

3.708 ± 0.049

4.256 ± 0.052

2.22 ± 0.031

5.982 ± 0.062

6.596 ± 0.063

4.709 ± 0.049

6.767 ± 0.056

1.194 ± 0.033

2.955 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski