Equus asinus papillomavirus 1
Average proteome isoelectric point is 5.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W5ZT83|W5ZT83_9PAPI Regulatory protein E2 OS=Equus asinus papillomavirus 1 OX=1163703 GN=E2 PE=3 SV=1
MM1 pKa = 7.95 PILARR6 pKa = 11.84 SRR8 pKa = 11.84 KK9 pKa = 9.0 RR10 pKa = 11.84 RR11 pKa = 11.84 AAPQDD16 pKa = 3.94 LYY18 pKa = 10.47 PACKK22 pKa = 9.38 QANTCPPDD30 pKa = 3.52 VLAKK34 pKa = 9.95 MEE36 pKa = 4.51 NDD38 pKa = 2.89 TWADD42 pKa = 4.33 RR43 pKa = 11.84 ILKK46 pKa = 7.95 WASGFLYY53 pKa = 10.39 FGNLGISTGRR63 pKa = 11.84 GTGGGFGYY71 pKa = 10.92 APIGGSGVATGGRR84 pKa = 11.84 TVVRR88 pKa = 11.84 PGIGANVVVPGEE100 pKa = 4.03 AVPTEE105 pKa = 4.18 TLGPLDD111 pKa = 4.37 PSVIPLDD118 pKa = 3.68 TLPSVEE124 pKa = 4.88 PDD126 pKa = 3.1 VLLPPGRR133 pKa = 11.84 PEE135 pKa = 3.86 VYY137 pKa = 9.77 EE138 pKa = 4.66 DD139 pKa = 3.53 SLGPRR144 pKa = 11.84 IPPEE148 pKa = 4.26 RR149 pKa = 11.84 LPAAYY154 pKa = 9.97 DD155 pKa = 3.53 EE156 pKa = 5.57 DD157 pKa = 4.63 GPPVHH162 pKa = 6.52 VPAVPADD169 pKa = 3.77 PAVHH173 pKa = 6.63 EE174 pKa = 4.45 GTPAVLEE181 pKa = 4.5 VPTEE185 pKa = 3.88 QAIVSHH191 pKa = 5.79 SQFANPAFEE200 pKa = 4.43 ASIVDD205 pKa = 3.25 ITGAADD211 pKa = 3.03 ISGQQSITVHH221 pKa = 6.33 HH222 pKa = 6.52 GAEE225 pKa = 4.02 GHH227 pKa = 6.75 IIGGDD232 pKa = 3.42 VLINPFLEE240 pKa = 4.28 STEE243 pKa = 4.18 TLEE246 pKa = 5.12 GEE248 pKa = 4.57 HH249 pKa = 6.47 IPLRR253 pKa = 11.84 SFTSSMRR260 pKa = 11.84 DD261 pKa = 2.93 EE262 pKa = 4.38 FEE264 pKa = 4.49 YY265 pKa = 11.38 NEE267 pKa = 4.1 EE268 pKa = 4.26 TPLQSTPTEE277 pKa = 4.09 RR278 pKa = 11.84 PAPRR282 pKa = 11.84 GRR284 pKa = 11.84 RR285 pKa = 11.84 LGNTRR290 pKa = 11.84 YY291 pKa = 8.79 FRR293 pKa = 11.84 QVKK296 pKa = 9.75 VEE298 pKa = 3.93 DD299 pKa = 3.94 TLFLHH304 pKa = 6.81 HH305 pKa = 6.77 PQRR308 pKa = 11.84 LVTFDD313 pKa = 3.34 NPVYY317 pKa = 10.32 EE318 pKa = 5.07 GEE320 pKa = 4.14 DD321 pKa = 3.28 SYY323 pKa = 12.25 GFAEE327 pKa = 4.86 ALDD330 pKa = 4.22 PSAPYY335 pKa = 9.85 PDD337 pKa = 5.15 LDD339 pKa = 4.03 FRR341 pKa = 11.84 DD342 pKa = 4.05 LKK344 pKa = 10.9 SLSRR348 pKa = 11.84 VEE350 pKa = 4.16 YY351 pKa = 10.43 GRR353 pKa = 11.84 NPQGLVRR360 pKa = 11.84 VSRR363 pKa = 11.84 VGKK366 pKa = 10.15 AHH368 pKa = 5.38 GTMLRR373 pKa = 11.84 SGLRR377 pKa = 11.84 IGPEE381 pKa = 3.39 THH383 pKa = 6.22 YY384 pKa = 11.2 FMDD387 pKa = 4.06 LSEE390 pKa = 4.37 IAVEE394 pKa = 4.61 SEE396 pKa = 4.06 LSLHH400 pKa = 6.81 AEE402 pKa = 4.16 TSHH405 pKa = 5.46 MVGRR409 pKa = 11.84 EE410 pKa = 3.93 AAQVTPLMEE419 pKa = 5.14 EE420 pKa = 3.44 ISLISEE426 pKa = 4.39 AGSVYY431 pKa = 10.61 SDD433 pKa = 3.54 SDD435 pKa = 3.98 LLDD438 pKa = 4.01 VIEE441 pKa = 5.25 DD442 pKa = 3.47 LGEE445 pKa = 4.09 RR446 pKa = 11.84 LQLVIGSGRR455 pKa = 11.84 NRR457 pKa = 11.84 LQVPLPLRR465 pKa = 11.84 NLSYY469 pKa = 11.26 LDD471 pKa = 3.25 TSGIFVGYY479 pKa = 9.01 VIPTEE484 pKa = 4.6 GQDD487 pKa = 3.04 STQRR491 pKa = 11.84 SDD493 pKa = 3.32 IEE495 pKa = 4.22 DD496 pKa = 3.38 VSEE499 pKa = 4.28 VFSAADD505 pKa = 3.97 LSPSFDD511 pKa = 3.28 WPMTDD516 pKa = 4.07 LDD518 pKa = 4.57 ADD520 pKa = 4.4 FVLHH524 pKa = 6.74 PSLLKK529 pKa = 10.06 RR530 pKa = 11.84 RR531 pKa = 11.84 RR532 pKa = 11.84 RR533 pKa = 11.84 FYY535 pKa = 10.58 WSFADD540 pKa = 4.07 GGLASRR546 pKa = 11.84 TKK548 pKa = 10.81
Molecular weight: 59.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.773
IPC2_protein 4.749
IPC_protein 4.711
Toseland 4.584
ProMoST 4.825
Dawson 4.673
Bjellqvist 4.813
Wikipedia 4.533
Rodwell 4.571
Grimsley 4.495
Solomon 4.673
Lehninger 4.622
Nozaki 4.774
DTASelect 4.94
Thurlkill 4.584
EMBOSS 4.558
Sillero 4.851
Patrickios 3.986
IPC_peptide 4.673
IPC2_peptide 4.838
IPC2.peptide.svr19 4.8
Protein with the highest isoelectric point:
>tr|W6A1G2|W6A1G2_9PAPI Replication protein E1 OS=Equus asinus papillomavirus 1 OX=1163703 GN=E1 PE=3 SV=1
MM1 pKa = 7.27 TALQEE6 pKa = 4.34 RR7 pKa = 11.84 LDD9 pKa = 4.08 SVQEE13 pKa = 3.84 QQLQIIEE20 pKa = 4.3 TGTTKK25 pKa = 10.75 LDD27 pKa = 3.68 DD28 pKa = 4.84 LLVFWRR34 pKa = 11.84 SVRR37 pKa = 11.84 QEE39 pKa = 3.37 NALLHH44 pKa = 6.05 FARR47 pKa = 11.84 QKK49 pKa = 10.05 GLSRR53 pKa = 11.84 IDD55 pKa = 3.58 LTPVPSLAASACKK68 pKa = 9.89 AKK70 pKa = 10.31 QAIEE74 pKa = 4.2 IEE76 pKa = 4.24 LSLEE80 pKa = 4.39 GIKK83 pKa = 10.84 NSGWDD88 pKa = 3.71 DD89 pKa = 4.21 EE90 pKa = 4.67 PWTLQDD96 pKa = 4.35 GSWDD100 pKa = 3.58 RR101 pKa = 11.84 YY102 pKa = 9.01 AASPEE107 pKa = 4.03 GAFKK111 pKa = 10.94 KK112 pKa = 10.55 NPAVVEE118 pKa = 4.55 VIFDD122 pKa = 4.08 EE123 pKa = 4.47 NPEE126 pKa = 3.87 NAVWYY131 pKa = 6.2 THH133 pKa = 6.42 WEE135 pKa = 3.98 VLYY138 pKa = 9.9 VTDD141 pKa = 3.94 GVKK144 pKa = 8.75 WHH146 pKa = 6.55 RR147 pKa = 11.84 TTGRR151 pKa = 11.84 VCANGLFYY159 pKa = 10.78 EE160 pKa = 4.77 LGGEE164 pKa = 3.88 QTFYY168 pKa = 11.52 EE169 pKa = 4.72 RR170 pKa = 11.84 FDD172 pKa = 4.54 LDD174 pKa = 3.37 AQKK177 pKa = 11.16 YY178 pKa = 6.09 STGGTWTVTFQGEE191 pKa = 4.66 TMTGSLVSSSTDD203 pKa = 3.25 GVDD206 pKa = 3.59 GEE208 pKa = 4.79 ARR210 pKa = 11.84 PPSPMPASTACGEE223 pKa = 4.1 RR224 pKa = 11.84 RR225 pKa = 11.84 DD226 pKa = 4.15 NLSTPAEE233 pKa = 4.41 PDD235 pKa = 2.94 SSSAVSSNFTSSGGRR250 pKa = 11.84 SRR252 pKa = 11.84 GRR254 pKa = 11.84 ANRR257 pKa = 11.84 GCPYY261 pKa = 10.09 TRR263 pKa = 11.84 TEE265 pKa = 4.21 GPRR268 pKa = 11.84 VPKK271 pKa = 10.44 VVSAPCYY278 pKa = 9.69 RR279 pKa = 11.84 PRR281 pKa = 11.84 GVQAQLGSTPPLVSPADD298 pKa = 3.57 PLGRR302 pKa = 11.84 SSAGEE307 pKa = 4.17 RR308 pKa = 11.84 PPGAAGHH315 pKa = 6.17 SGSVGNAVPPAAPPASPFLQGSRR338 pKa = 11.84 CPVILVTGSCNQVKK352 pKa = 9.74 CWRR355 pKa = 11.84 LRR357 pKa = 11.84 CRR359 pKa = 11.84 RR360 pKa = 11.84 HH361 pKa = 5.06 HH362 pKa = 5.27 RR363 pKa = 11.84 HH364 pKa = 5.93 RR365 pKa = 11.84 YY366 pKa = 8.44 HH367 pKa = 7.2 RR368 pKa = 11.84 VTSTWYY374 pKa = 8.43 ITGDD378 pKa = 3.38 QGNDD382 pKa = 2.83 RR383 pKa = 11.84 AGPSTVMVMFTDD395 pKa = 4.24 DD396 pKa = 3.26 AQRR399 pKa = 11.84 ADD401 pKa = 4.05 FMAKK405 pKa = 9.6 VSFPPGVSCTPLTLSTT421 pKa = 4.27
Molecular weight: 45.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.474
IPC2_protein 6.491
IPC_protein 6.576
Toseland 6.547
ProMoST 6.985
Dawson 7.088
Bjellqvist 7.073
Wikipedia 7.044
Rodwell 7.088
Grimsley 6.62
Solomon 7.102
Lehninger 7.117
Nozaki 7.41
DTASelect 7.38
Thurlkill 7.424
EMBOSS 7.424
Sillero 7.541
Patrickios 4.291
IPC_peptide 7.117
IPC2_peptide 7.161
IPC2.peptide.svr19 6.95
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2204
99
625
440.8
48.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.258 ± 0.379
1.906 ± 0.685
5.944 ± 0.255
6.488 ± 0.39
4.083 ± 0.456
7.123 ± 0.705
2.269 ± 0.303
3.539 ± 0.441
3.857 ± 0.662
8.848 ± 0.556
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.497 ± 0.137
3.993 ± 0.631
6.987 ± 0.708
3.766 ± 0.315
6.67 ± 0.246
7.577 ± 0.706
6.216 ± 0.437
6.261 ± 0.614
1.679 ± 0.306
3.04 ± 0.273
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here