Armatimonadetes bacterium Uphvl-Ar1
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3008 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y0GWU2|A0A1Y0GWU2_9BACT Uncharacterized protein OS=Armatimonadetes bacterium Uphvl-Ar1 OX=2004467 GN=CCB80_03225 PE=4 SV=1
MM1 pKa = 7.8 TDD3 pKa = 3.23 QEE5 pKa = 5.03 HH6 pKa = 6.42 YY7 pKa = 10.99 LPDD10 pKa = 3.91 GLVDD14 pKa = 4.94 LEE16 pKa = 5.13 PGDD19 pKa = 3.86 ILCWDD24 pKa = 4.62 LQPEE28 pKa = 4.15 PGEE31 pKa = 4.3 YY32 pKa = 10.64 YY33 pKa = 10.81 LVATSKK39 pKa = 10.68 FDD41 pKa = 4.69 AIVSEE46 pKa = 4.14 LLGAKK51 pKa = 9.54 KK52 pKa = 10.31 ALADD56 pKa = 3.59 AVEE59 pKa = 4.85 LGFSSIDD66 pKa = 3.15 AMQAIWDD73 pKa = 4.02 SDD75 pKa = 4.34 SEE77 pKa = 4.48 VQACGAGATAMPEE90 pKa = 3.98 LYY92 pKa = 10.41 NYY94 pKa = 10.55 LNHH97 pKa = 6.28 QRR99 pKa = 11.84 FDD101 pKa = 3.82 HH102 pKa = 7.14 VDD104 pKa = 3.3 GQQPSDD110 pKa = 3.71 QPVKK114 pKa = 10.53 EE115 pKa = 4.45 SPQEE119 pKa = 3.83 EE120 pKa = 4.48 ASS122 pKa = 3.49
Molecular weight: 13.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.834
IPC_protein 3.795
Toseland 3.592
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.101
Thurlkill 3.643
EMBOSS 3.719
Sillero 3.923
Patrickios 0.947
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A1Y0H4I5|A0A1Y0H4I5_9BACT Uncharacterized protein OS=Armatimonadetes bacterium Uphvl-Ar1 OX=2004467 GN=CCB80_13425 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 8.16 RR12 pKa = 11.84 HH13 pKa = 5.28 KK14 pKa = 9.84 KK15 pKa = 8.68 HH16 pKa = 5.29 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MRR23 pKa = 11.84 TNDD26 pKa = 3.23 GQNVLKK32 pKa = 10.57 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 TKK37 pKa = 10.18 GRR39 pKa = 11.84 HH40 pKa = 4.68 RR41 pKa = 11.84 LGAA44 pKa = 3.91
Molecular weight: 5.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.425
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.369
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.087
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3008
0
3008
893138
36
2344
296.9
32.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.173 ± 0.046
0.889 ± 0.015
5.392 ± 0.032
6.346 ± 0.048
4.17 ± 0.03
8.366 ± 0.048
1.853 ± 0.021
5.765 ± 0.034
4.483 ± 0.039
9.412 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.521 ± 0.023
3.715 ± 0.035
4.895 ± 0.038
3.716 ± 0.028
5.73 ± 0.04
6.408 ± 0.038
5.785 ± 0.043
7.386 ± 0.042
1.465 ± 0.022
2.53 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here