Gokushovirinae GAIR4

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Gokushovirinae; unclassified Gokushovirinae

Average proteome isoelectric point is 8.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M5KZP2|A0A0M5KZP2_9VIRU Putative major capsid protein OS=Gokushovirinae GAIR4 OX=1701404 PE=3 SV=1
MM1 pKa = 7.49TMMHH5 pKa = 7.2KK6 pKa = 10.27NRR8 pKa = 11.84SVDD11 pKa = 3.13PHH13 pKa = 6.72RR14 pKa = 11.84FAMIPHH20 pKa = 6.93ADD22 pKa = 3.12IPRR25 pKa = 11.84AKK27 pKa = 10.09FDD29 pKa = 3.38RR30 pKa = 11.84QFTHH34 pKa = 6.1KK35 pKa = 7.96TTFDD39 pKa = 2.97AGYY42 pKa = 9.58LVPVYY47 pKa = 10.54VDD49 pKa = 3.45EE50 pKa = 4.56VLPGDD55 pKa = 3.93TFNLNMTAFARR66 pKa = 11.84LATPFFLLWIIWFWIVFFFFVPNRR90 pKa = 11.84LIWDD94 pKa = 3.67NWQKK98 pKa = 11.19FMVSSVTLVIRR109 pKa = 11.84LLCCSSTSFSRR120 pKa = 11.84RR121 pKa = 11.84WLCCWFFARR130 pKa = 11.84LYY132 pKa = 11.03GFTNCWSGWCWQYY145 pKa = 11.31YY146 pKa = 9.2FPLRR150 pKa = 11.84FFTRR154 pKa = 11.84AYY156 pKa = 10.45NLIWNEE162 pKa = 3.57WFRR165 pKa = 11.84DD166 pKa = 3.63QNLQNSVTVDD176 pKa = 3.12TGDD179 pKa = 4.08GPDD182 pKa = 3.83ASASTNYY189 pKa = 9.56TLLRR193 pKa = 11.84RR194 pKa = 11.84GKK196 pKa = 8.75RR197 pKa = 11.84HH198 pKa = 7.34DD199 pKa = 4.13YY200 pKa = 8.15FTSALPWPQKK210 pKa = 10.37GSSVTLPLGTSAPIKK225 pKa = 10.64SDD227 pKa = 3.11TTQVQFKK234 pKa = 10.79YY235 pKa = 10.74GATTFNSVSRR245 pKa = 11.84TDD247 pKa = 3.57VNVLGFNGAAVGAAGQAISFGANTGLYY274 pKa = 10.29ADD276 pKa = 5.23LSQATAATINQLRR289 pKa = 11.84QSFQIQKK296 pKa = 10.32LLEE299 pKa = 4.31RR300 pKa = 11.84DD301 pKa = 3.28ARR303 pKa = 11.84GGTRR307 pKa = 11.84YY308 pKa = 8.64TEE310 pKa = 4.53IIRR313 pKa = 11.84AHH315 pKa = 6.54FGVISPDD322 pKa = 3.07ARR324 pKa = 11.84LQRR327 pKa = 11.84PEE329 pKa = 3.87YY330 pKa = 10.14LGGGSTPVIINPIAQTSGTGVTGGSTPLANLAGVGTVLASGHH372 pKa = 6.33GFTQSFTEE380 pKa = 3.85HH381 pKa = 5.82GVIIGLVSIRR391 pKa = 11.84ADD393 pKa = 3.22LTYY396 pKa = 10.62QQGLHH401 pKa = 6.09RR402 pKa = 11.84MWSRR406 pKa = 11.84STRR409 pKa = 11.84YY410 pKa = 10.23DD411 pKa = 3.49FYY413 pKa = 11.54FPAFAMLGEE422 pKa = 4.0QPVYY426 pKa = 10.82NRR428 pKa = 11.84EE429 pKa = 3.98LYY431 pKa = 10.09IDD433 pKa = 4.42GSANDD438 pKa = 4.13GNVFGYY444 pKa = 7.33QEE446 pKa = 3.36RR447 pKa = 11.84WAEE450 pKa = 3.88YY451 pKa = 9.71RR452 pKa = 11.84YY453 pKa = 10.32KK454 pKa = 10.54PSQISSLFKK463 pKa = 9.81STSAGTIDD471 pKa = 3.86AWHH474 pKa = 7.28LAQKK478 pKa = 8.14FTSLPTLNSTFIQEE492 pKa = 4.33NPPVSRR498 pKa = 11.84VVAVGAAANGQQFIFDD514 pKa = 4.05SFFHH518 pKa = 6.06NVVARR523 pKa = 11.84PMPLYY528 pKa = 10.18SVPGMIDD535 pKa = 3.02HH536 pKa = 7.39FF537 pKa = 4.83

Molecular weight: 60.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M5KZP2|A0A0M5KZP2_9VIRU Putative major capsid protein OS=Gokushovirinae GAIR4 OX=1701404 PE=3 SV=1
MM1 pKa = 7.49TMMHH5 pKa = 7.2KK6 pKa = 10.27NRR8 pKa = 11.84SVDD11 pKa = 3.13PHH13 pKa = 6.72RR14 pKa = 11.84FAMIPHH20 pKa = 6.93ADD22 pKa = 3.12IPRR25 pKa = 11.84AKK27 pKa = 10.09FDD29 pKa = 3.38RR30 pKa = 11.84QFTHH34 pKa = 6.1KK35 pKa = 7.96TTFDD39 pKa = 2.97AGYY42 pKa = 9.58LVPVYY47 pKa = 10.54VDD49 pKa = 3.45EE50 pKa = 4.56VLPGDD55 pKa = 3.93TFNLNMTAFARR66 pKa = 11.84LATPFFLLWIIWFWIVFFFFVPNRR90 pKa = 11.84LIWDD94 pKa = 3.67NWQKK98 pKa = 11.19FMVSSVTLVIRR109 pKa = 11.84LLCCSSTSFSRR120 pKa = 11.84RR121 pKa = 11.84WLCCWFFARR130 pKa = 11.84LYY132 pKa = 11.03GFTNCWSGWCWQYY145 pKa = 11.31YY146 pKa = 9.2FPLRR150 pKa = 11.84FFTRR154 pKa = 11.84AYY156 pKa = 10.45NLIWNEE162 pKa = 3.57WFRR165 pKa = 11.84DD166 pKa = 3.63QNLQNSVTVDD176 pKa = 3.12TGDD179 pKa = 4.08GPDD182 pKa = 3.83ASASTNYY189 pKa = 9.56TLLRR193 pKa = 11.84RR194 pKa = 11.84GKK196 pKa = 8.75RR197 pKa = 11.84HH198 pKa = 7.34DD199 pKa = 4.13YY200 pKa = 8.15FTSALPWPQKK210 pKa = 10.37GSSVTLPLGTSAPIKK225 pKa = 10.64SDD227 pKa = 3.11TTQVQFKK234 pKa = 10.79YY235 pKa = 10.74GATTFNSVSRR245 pKa = 11.84TDD247 pKa = 3.57VNVLGFNGAAVGAAGQAISFGANTGLYY274 pKa = 10.29ADD276 pKa = 5.23LSQATAATINQLRR289 pKa = 11.84QSFQIQKK296 pKa = 10.32LLEE299 pKa = 4.31RR300 pKa = 11.84DD301 pKa = 3.28ARR303 pKa = 11.84GGTRR307 pKa = 11.84YY308 pKa = 8.64TEE310 pKa = 4.53IIRR313 pKa = 11.84AHH315 pKa = 6.54FGVISPDD322 pKa = 3.07ARR324 pKa = 11.84LQRR327 pKa = 11.84PEE329 pKa = 3.87YY330 pKa = 10.14LGGGSTPVIINPIAQTSGTGVTGGSTPLANLAGVGTVLASGHH372 pKa = 6.33GFTQSFTEE380 pKa = 3.85HH381 pKa = 5.82GVIIGLVSIRR391 pKa = 11.84ADD393 pKa = 3.22LTYY396 pKa = 10.62QQGLHH401 pKa = 6.09RR402 pKa = 11.84MWSRR406 pKa = 11.84STRR409 pKa = 11.84YY410 pKa = 10.23DD411 pKa = 3.49FYY413 pKa = 11.54FPAFAMLGEE422 pKa = 4.0QPVYY426 pKa = 10.82NRR428 pKa = 11.84EE429 pKa = 3.98LYY431 pKa = 10.09IDD433 pKa = 4.42GSANDD438 pKa = 4.13GNVFGYY444 pKa = 7.33QEE446 pKa = 3.36RR447 pKa = 11.84WAEE450 pKa = 3.88YY451 pKa = 9.71RR452 pKa = 11.84YY453 pKa = 10.32KK454 pKa = 10.54PSQISSLFKK463 pKa = 9.81STSAGTIDD471 pKa = 3.86AWHH474 pKa = 7.28LAQKK478 pKa = 8.14FTSLPTLNSTFIQEE492 pKa = 4.33NPPVSRR498 pKa = 11.84VVAVGAAANGQQFIFDD514 pKa = 4.05SFFHH518 pKa = 6.06NVVARR523 pKa = 11.84PMPLYY528 pKa = 10.18SVPGMIDD535 pKa = 3.02HH536 pKa = 7.39FF537 pKa = 4.83

Molecular weight: 60.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

537

537

537

537.0

60.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.194 ± 0.0

1.117 ± 0.0

4.469 ± 0.0

2.048 ± 0.0

8.007 ± 0.0

7.635 ± 0.0

2.235 ± 0.0

5.028 ± 0.0

2.235 ± 0.0

7.635 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.862 ± 0.0

4.655 ± 0.0

5.028 ± 0.0

4.842 ± 0.0

6.145 ± 0.0

7.635 ± 0.0

8.007 ± 0.0

6.331 ± 0.0

2.98 ± 0.0

3.911 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski