Microbacterium phage Hendrix

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rogerhendrixvirus; Microbacterium virus Hendrix

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 155 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UUC3|A0A2U8UUC3_9CAUD Uncharacterized protein OS=Microbacterium phage Hendrix OX=2182341 GN=103 PE=4 SV=1
MM1 pKa = 7.1LHH3 pKa = 6.88LYY5 pKa = 9.68YY6 pKa = 10.23RR7 pKa = 11.84EE8 pKa = 4.15EE9 pKa = 4.55GPPGLGCNTMATLMSADD26 pKa = 3.84EE27 pKa = 4.3LFKK30 pKa = 10.37RR31 pKa = 11.84TQIARR36 pKa = 11.84DD37 pKa = 3.44AYY39 pKa = 9.01MQTGFLGGYY48 pKa = 9.32RR49 pKa = 11.84EE50 pKa = 4.15VRR52 pKa = 11.84SDD54 pKa = 3.34LVNWLEE60 pKa = 4.23HH61 pKa = 5.49NTDD64 pKa = 4.34DD65 pKa = 4.79EE66 pKa = 4.57VQLAYY71 pKa = 10.69LPGMFDD77 pKa = 3.46VATLAPFASEE87 pKa = 4.42VYY89 pKa = 10.04NYY91 pKa = 10.41AWQEE95 pKa = 3.84LLNGLEE101 pKa = 4.05EE102 pKa = 4.36VEE104 pKa = 4.6RR105 pKa = 11.84NAA107 pKa = 5.11

Molecular weight: 12.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UUC8|A0A2U8UUC8_9CAUD Uncharacterized protein OS=Microbacterium phage Hendrix OX=2182341 GN=99 PE=4 SV=1
MM1 pKa = 7.58FGINWSRR8 pKa = 11.84RR9 pKa = 11.84IRR11 pKa = 11.84RR12 pKa = 11.84ATVRR16 pKa = 11.84AAKK19 pKa = 10.27KK20 pKa = 6.95STRR23 pKa = 11.84WATKK27 pKa = 9.58PYY29 pKa = 9.11RR30 pKa = 11.84TEE32 pKa = 4.03KK33 pKa = 10.81KK34 pKa = 10.07NLAAAKK40 pKa = 9.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84QTWRR47 pKa = 11.84SRR49 pKa = 3.16

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

155

0

155

31207

40

1233

201.3

22.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.344 ± 0.398

0.84 ± 0.1

6.319 ± 0.213

7.335 ± 0.312

2.865 ± 0.128

7.7 ± 0.177

2.076 ± 0.148

4.832 ± 0.211

3.804 ± 0.189

7.812 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.637 ± 0.13

3.092 ± 0.157

5.611 ± 0.367

3.772 ± 0.123

6.662 ± 0.271

5.752 ± 0.239

6.736 ± 0.346

7.191 ± 0.218

2.086 ± 0.14

2.535 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski