Pacific flying fox faeces associated circular DNA virus-15
Average proteome isoelectric point is 7.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140CTW4|A0A140CTW4_9VIRU Putative capsid protein OS=Pacific flying fox faeces associated circular DNA virus-15 OX=1796010 PE=4 SV=1
MM1 pKa = 7.69 DD2 pKa = 4.21 TFLTAHH8 pKa = 5.72 QEE10 pKa = 4.0 EE11 pKa = 5.04 EE12 pKa = 3.88 PRR14 pKa = 11.84 YY15 pKa = 9.01 PGSDD19 pKa = 3.5 VEE21 pKa = 5.8 DD22 pKa = 5.14 DD23 pKa = 4.55 SLLCLEE29 pKa = 5.44 GPLSPEE35 pKa = 4.47 DD36 pKa = 3.74 EE37 pKa = 4.23 TLEE40 pKa = 4.28 APKK43 pKa = 10.27 EE44 pKa = 4.15 KK45 pKa = 10.29 PKK47 pKa = 10.79 SEE49 pKa = 4.01 KK50 pKa = 10.3 KK51 pKa = 10.16 KK52 pKa = 10.37 GSFRR56 pKa = 11.84 LRR58 pKa = 11.84 GTQFILTFPQCDD70 pKa = 3.43 VKK72 pKa = 11.28 KK73 pKa = 10.06 EE74 pKa = 3.97 VAAEE78 pKa = 4.36 RR79 pKa = 11.84 IDD81 pKa = 3.81 IAWGKK86 pKa = 10.31 DD87 pKa = 2.83 LTGYY91 pKa = 7.57 VVCEE95 pKa = 4.14 EE96 pKa = 3.98 NHH98 pKa = 6.93 KK99 pKa = 11.14 DD100 pKa = 3.76 GTPHH104 pKa = 6.0 LHH106 pKa = 6.47 VYY108 pKa = 10.74 LKK110 pKa = 10.76 FEE112 pKa = 4.08 NRR114 pKa = 11.84 KK115 pKa = 9.5 SFSKK119 pKa = 10.63 VDD121 pKa = 3.46 CFDD124 pKa = 5.64 FIGQKK129 pKa = 9.69 HH130 pKa = 5.79 GNYY133 pKa = 9.29 QVVRR137 pKa = 11.84 STLKK141 pKa = 10.3 SIEE144 pKa = 4.12 YY145 pKa = 7.57 VTKK148 pKa = 10.49 GGNYY152 pKa = 7.25 VAKK155 pKa = 10.24 GVDD158 pKa = 3.27 VEE160 pKa = 4.64 SIKK163 pKa = 10.75 KK164 pKa = 10.01 KK165 pKa = 10.18 KK166 pKa = 9.97 APKK169 pKa = 8.5 NQEE172 pKa = 3.64 IAKK175 pKa = 8.89 MLLDD179 pKa = 4.32 GKK181 pKa = 11.36 SMAEE185 pKa = 3.89 INEE188 pKa = 4.17 DD189 pKa = 3.04 HH190 pKa = 6.97 CGYY193 pKa = 11.67 VMINKK198 pKa = 9.75 RR199 pKa = 11.84 KK200 pKa = 8.76 IEE202 pKa = 4.18 EE203 pKa = 4.02 YY204 pKa = 8.22 EE205 pKa = 4.01 TWVKK209 pKa = 10.63 CEE211 pKa = 4.36 KK212 pKa = 10.13 IKK214 pKa = 10.62 KK215 pKa = 10.14 SKK217 pKa = 10.18 IDD219 pKa = 4.06 WIPPSLDD226 pKa = 2.94 GLTDD230 pKa = 3.83 ANLQIATWICSNIRR244 pKa = 11.84 QNRR247 pKa = 11.84 AFKK250 pKa = 10.67 APQLYY255 pKa = 9.78 IHH257 pKa = 7.06 GPRR260 pKa = 11.84 NLGKK264 pKa = 8.97 TSLVEE269 pKa = 3.61 WLEE272 pKa = 4.14 RR273 pKa = 11.84 SLSVYY278 pKa = 10.53 HH279 pKa = 6.21 MPTMEE284 pKa = 5.29 DD285 pKa = 4.19 FYY287 pKa = 11.65 DD288 pKa = 4.75 LYY290 pKa = 11.43 SDD292 pKa = 5.57 DD293 pKa = 4.25 YY294 pKa = 11.07 DD295 pKa = 3.96 LVVIDD300 pKa = 4.45 EE301 pKa = 4.76 FKK303 pKa = 10.78 GQKK306 pKa = 8.92 TIQFLNQFLQGSVMPIRR323 pKa = 11.84 KK324 pKa = 9.42 KK325 pKa = 10.76 GSQGDD330 pKa = 3.5 KK331 pKa = 10.7 SKK333 pKa = 10.95 NLPVVILSNYY343 pKa = 7.45 TLSEE347 pKa = 4.64 CYY349 pKa = 10.42 VKK351 pKa = 10.68 AANDD355 pKa = 3.31 GRR357 pKa = 11.84 LNTLEE362 pKa = 4.07 CRR364 pKa = 11.84 LEE366 pKa = 3.97 IVEE369 pKa = 4.24 VDD371 pKa = 3.83 SFIDD375 pKa = 4.29 FYY377 pKa = 11.55 KK378 pKa = 10.84 DD379 pKa = 3.16 RR380 pKa = 11.84 NDD382 pKa = 4.63 LII384 pKa = 5.54
Molecular weight: 44.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.694
IPC2_protein 5.741
IPC_protein 5.766
Toseland 6.008
ProMoST 6.071
Dawson 5.97
Bjellqvist 5.995
Wikipedia 5.957
Rodwell 5.944
Grimsley 6.109
Solomon 5.97
Lehninger 5.957
Nozaki 6.211
DTASelect 6.389
Thurlkill 6.364
EMBOSS 6.326
Sillero 6.313
Patrickios 4.228
IPC_peptide 5.982
IPC2_peptide 6.249
IPC2.peptide.svr19 6.358
Protein with the highest isoelectric point:
>tr|A0A140CTW4|A0A140CTW4_9VIRU Putative capsid protein OS=Pacific flying fox faeces associated circular DNA virus-15 OX=1796010 PE=4 SV=1
MM1 pKa = 7.01 VGYY4 pKa = 10.46 VRR6 pKa = 11.84 TITYY10 pKa = 8.53 RR11 pKa = 11.84 RR12 pKa = 11.84 PYY14 pKa = 9.26 GSVYY18 pKa = 10.66 GPYY21 pKa = 10.41 NKK23 pKa = 9.83 YY24 pKa = 10.08 ARR26 pKa = 11.84 SRR28 pKa = 11.84 RR29 pKa = 11.84 IVNTAAKK36 pKa = 10.18 LLRR39 pKa = 11.84 KK40 pKa = 9.96 RR41 pKa = 11.84 MGGGPRR47 pKa = 11.84 APLATRR53 pKa = 11.84 GWYY56 pKa = 10.31 GNTYY60 pKa = 9.4 GKK62 pKa = 10.46 RR63 pKa = 11.84 GGEE66 pKa = 3.84 LKK68 pKa = 11.01 YY69 pKa = 10.33 IDD71 pKa = 4.43 VDD73 pKa = 3.82 NVPAALPVGGTVDD86 pKa = 3.62 VLNAIASGAGIDD98 pKa = 3.77 EE99 pKa = 4.15 RR100 pKa = 11.84 VGRR103 pKa = 11.84 QITLKK108 pKa = 10.71 SIFSRR113 pKa = 11.84 WGFYY117 pKa = 10.49 PSTTTSAPQGTIVRR131 pKa = 11.84 VLIVYY136 pKa = 8.02 DD137 pKa = 3.74 AQANSAASPPAVNEE151 pKa = 3.8 ILATGTWDD159 pKa = 4.5 SPMNLNNRR167 pKa = 11.84 EE168 pKa = 3.83 RR169 pKa = 11.84 FKK171 pKa = 10.77 IITEE175 pKa = 4.19 FKK177 pKa = 8.16 VTVGATLYY185 pKa = 9.92 TAGALTAGSPVPRR198 pKa = 11.84 VVEE201 pKa = 4.4 KK202 pKa = 10.94 YY203 pKa = 10.22 KK204 pKa = 10.57 KK205 pKa = 10.69 LNMTTTYY212 pKa = 10.66 SGPNNTNANIATGAIYY228 pKa = 10.68 VVTIPSLNNTVVADD242 pKa = 3.81 YY243 pKa = 9.3 YY244 pKa = 11.21 HH245 pKa = 6.69 RR246 pKa = 11.84 TRR248 pKa = 11.84 YY249 pKa = 8.48 MDD251 pKa = 3.49 CC252 pKa = 4.13
Molecular weight: 27.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.619
IPC2_protein 9.838
IPC_protein 10.306
Toseland 9.94
ProMoST 9.853
Dawson 10.262
Bjellqvist 10.028
Wikipedia 10.511
Rodwell 10.438
Grimsley 10.379
Solomon 10.292
Lehninger 10.218
Nozaki 9.94
DTASelect 10.014
Thurlkill 10.072
EMBOSS 10.379
Sillero 10.175
Patrickios 5.016
IPC_peptide 10.277
IPC2_peptide 8.902
IPC2.peptide.svr19 8.666
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
636
252
384
318.0
35.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.289 ± 2.21
1.572 ± 0.716
5.818 ± 1.609
5.975 ± 2.188
2.987 ± 0.852
7.233 ± 1.395
1.415 ± 0.62
6.132 ± 0.109
8.176 ± 2.32
7.39 ± 1.117
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.887 ± 0.059
5.346 ± 0.852
4.874 ± 0.656
2.673 ± 0.902
5.503 ± 1.481
5.818 ± 0.401
6.761 ± 2.165
7.547 ± 0.962
1.258 ± 0.041
5.346 ± 1.094
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here