Amino acid dipepetide frequency for Gordonia phage Bakery

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.047AlaAla: 16.047 ± 1.338
1.398AlaCys: 1.398 ± 0.295
7.765AlaAsp: 7.765 ± 0.823
8.179AlaGlu: 8.179 ± 0.699
2.795AlaPhe: 2.795 ± 0.409
9.214AlaGly: 9.214 ± 0.949
2.588AlaHis: 2.588 ± 0.405
5.746AlaIle: 5.746 ± 0.631
4.193AlaLys: 4.193 ± 0.466
10.042AlaLeu: 10.042 ± 1.099
3.261AlaMet: 3.261 ± 0.337
3.417AlaAsn: 3.417 ± 0.455
7.299AlaPro: 7.299 ± 0.834
4.193AlaGln: 4.193 ± 0.412
9.525AlaArg: 9.525 ± 0.63
5.849AlaSer: 5.849 ± 0.503
7.092AlaThr: 7.092 ± 0.598
8.904AlaVal: 8.904 ± 0.737
2.536AlaTrp: 2.536 ± 0.397
2.329AlaTyr: 2.329 ± 0.331
0.0AlaXaa: 0.0 ± 0.0
Cys
1.242CysAla: 1.242 ± 0.272
0.207CysCys: 0.207 ± 0.123
1.035CysAsp: 1.035 ± 0.246
0.466CysGlu: 0.466 ± 0.18
0.259CysPhe: 0.259 ± 0.109
1.191CysGly: 1.191 ± 0.296
0.311CysHis: 0.311 ± 0.121
0.207CysIle: 0.207 ± 0.097
0.155CysLys: 0.155 ± 0.085
0.362CysLeu: 0.362 ± 0.154
0.104CysMet: 0.104 ± 0.089
0.362CysAsn: 0.362 ± 0.148
0.776CysPro: 0.776 ± 0.222
0.207CysGln: 0.207 ± 0.105
0.88CysArg: 0.88 ± 0.297
0.569CysSer: 0.569 ± 0.172
0.621CysThr: 0.621 ± 0.15
0.466CysVal: 0.466 ± 0.171
0.104CysTrp: 0.104 ± 0.077
0.052CysTyr: 0.052 ± 0.05
0.0CysXaa: 0.0 ± 0.0
Asp
8.334AspAla: 8.334 ± 0.579
0.621AspCys: 0.621 ± 0.193
5.746AspAsp: 5.746 ± 0.58
5.125AspGlu: 5.125 ± 0.622
1.605AspPhe: 1.605 ± 0.246
5.953AspGly: 5.953 ± 0.606
1.708AspHis: 1.708 ± 0.247
2.071AspIle: 2.071 ± 0.335
1.501AspLys: 1.501 ± 0.292
6.729AspLeu: 6.729 ± 0.526
1.139AspMet: 1.139 ± 0.203
2.019AspAsn: 2.019 ± 0.29
5.539AspPro: 5.539 ± 0.63
2.588AspGln: 2.588 ± 0.419
5.746AspArg: 5.746 ± 0.66
2.795AspSer: 2.795 ± 0.423
3.831AspThr: 3.831 ± 0.466
5.177AspVal: 5.177 ± 0.532
1.605AspTrp: 1.605 ± 0.277
1.915AspTyr: 1.915 ± 0.346
0.0AspXaa: 0.0 ± 0.0
Glu
6.988GluAla: 6.988 ± 0.507
0.569GluCys: 0.569 ± 0.178
3.106GluAsp: 3.106 ± 0.42
2.536GluGlu: 2.536 ± 0.462
1.967GluPhe: 1.967 ± 0.288
4.245GluGly: 4.245 ± 0.671
0.984GluHis: 0.984 ± 0.243
2.951GluIle: 2.951 ± 0.323
1.812GluLys: 1.812 ± 0.291
5.849GluLeu: 5.849 ± 0.599
0.88GluMet: 0.88 ± 0.191
1.864GluAsn: 1.864 ± 0.321
3.675GluPro: 3.675 ± 0.601
2.847GluGln: 2.847 ± 0.51
4.089GluArg: 4.089 ± 0.522
2.381GluSer: 2.381 ± 0.287
3.002GluThr: 3.002 ± 0.516
4.193GluVal: 4.193 ± 0.486
1.708GluTrp: 1.708 ± 0.402
1.191GluTyr: 1.191 ± 0.208
0.0GluXaa: 0.0 ± 0.0
Phe
3.313PheAla: 3.313 ± 0.379
0.104PheCys: 0.104 ± 0.075
2.329PheAsp: 2.329 ± 0.271
1.191PheGlu: 1.191 ± 0.235
0.776PhePhe: 0.776 ± 0.313
1.967PheGly: 1.967 ± 0.273
0.673PheHis: 0.673 ± 0.198
1.035PheIle: 1.035 ± 0.194
0.518PheLys: 0.518 ± 0.129
1.398PheLeu: 1.398 ± 0.249
0.621PheMet: 0.621 ± 0.224
0.828PheAsn: 0.828 ± 0.37
1.501PhePro: 1.501 ± 0.304
0.776PheGln: 0.776 ± 0.168
2.071PheArg: 2.071 ± 0.299
1.708PheSer: 1.708 ± 0.288
1.967PheThr: 1.967 ± 0.345
1.864PheVal: 1.864 ± 0.338
0.518PheTrp: 0.518 ± 0.141
0.259PheTyr: 0.259 ± 0.134
0.0PheXaa: 0.0 ± 0.0
Gly
8.127GlyAla: 8.127 ± 0.939
0.932GlyCys: 0.932 ± 0.261
5.125GlyAsp: 5.125 ± 0.558
4.762GlyGlu: 4.762 ± 0.417
2.329GlyPhe: 2.329 ± 0.338
9.266GlyGly: 9.266 ± 1.954
2.071GlyHis: 2.071 ± 0.467
3.986GlyIle: 3.986 ± 0.407
2.329GlyLys: 2.329 ± 0.346
5.901GlyLeu: 5.901 ± 0.563
1.708GlyMet: 1.708 ± 0.316
2.847GlyAsn: 2.847 ± 0.499
4.297GlyPro: 4.297 ± 0.458
3.52GlyGln: 3.52 ± 0.454
6.005GlyArg: 6.005 ± 0.522
3.934GlySer: 3.934 ± 0.539
4.762GlyThr: 4.762 ± 0.719
7.247GlyVal: 7.247 ± 0.6
1.76GlyTrp: 1.76 ± 0.255
1.553GlyTyr: 1.553 ± 0.23
0.0GlyXaa: 0.0 ± 0.0
His
2.226HisAla: 2.226 ± 0.409
0.104HisCys: 0.104 ± 0.067
1.708HisAsp: 1.708 ± 0.285
0.828HisGlu: 0.828 ± 0.21
0.414HisPhe: 0.414 ± 0.142
1.76HisGly: 1.76 ± 0.254
0.932HisHis: 0.932 ± 0.314
0.984HisIle: 0.984 ± 0.214
0.362HisLys: 0.362 ± 0.125
2.122HisLeu: 2.122 ± 0.322
0.207HisMet: 0.207 ± 0.097
0.776HisAsn: 0.776 ± 0.171
1.605HisPro: 1.605 ± 0.291
0.828HisGln: 0.828 ± 0.274
2.278HisArg: 2.278 ± 0.416
0.776HisSer: 0.776 ± 0.19
1.864HisThr: 1.864 ± 0.36
1.139HisVal: 1.139 ± 0.297
0.518HisTrp: 0.518 ± 0.151
0.362HisTyr: 0.362 ± 0.136
0.0HisXaa: 0.0 ± 0.0
Ile
5.798IleAla: 5.798 ± 0.515
0.155IleCys: 0.155 ± 0.078
3.624IleAsp: 3.624 ± 0.437
2.951IleGlu: 2.951 ± 0.401
0.725IlePhe: 0.725 ± 0.189
3.675IleGly: 3.675 ± 0.832
0.984IleHis: 0.984 ± 0.243
1.501IleIle: 1.501 ± 0.394
0.725IleLys: 0.725 ± 0.172
2.692IleLeu: 2.692 ± 0.454
0.621IleMet: 0.621 ± 0.207
0.984IleAsn: 0.984 ± 0.22
2.536IlePro: 2.536 ± 0.337
1.087IleGln: 1.087 ± 0.235
3.313IleArg: 3.313 ± 0.505
2.071IleSer: 2.071 ± 0.309
3.106IleThr: 3.106 ± 0.416
4.348IleVal: 4.348 ± 0.453
0.932IleTrp: 0.932 ± 0.214
0.621IleTyr: 0.621 ± 0.159
0.0IleXaa: 0.0 ± 0.0
Lys
3.313LysAla: 3.313 ± 0.463
0.259LysCys: 0.259 ± 0.134
1.76LysAsp: 1.76 ± 0.28
1.035LysGlu: 1.035 ± 0.243
0.828LysPhe: 0.828 ± 0.207
1.605LysGly: 1.605 ± 0.252
0.362LysHis: 0.362 ± 0.125
0.725LysIle: 0.725 ± 0.183
1.346LysLys: 1.346 ± 0.298
2.744LysLeu: 2.744 ± 0.403
0.725LysMet: 0.725 ± 0.194
0.984LysAsn: 0.984 ± 0.217
1.656LysPro: 1.656 ± 0.276
0.673LysGln: 0.673 ± 0.193
2.692LysArg: 2.692 ± 0.328
1.605LysSer: 1.605 ± 0.297
2.329LysThr: 2.329 ± 0.369
1.967LysVal: 1.967 ± 0.279
0.414LysTrp: 0.414 ± 0.126
0.518LysTyr: 0.518 ± 0.154
0.0LysXaa: 0.0 ± 0.0
Leu
10.664LeuAla: 10.664 ± 0.785
0.725LeuCys: 0.725 ± 0.19
6.367LeuAsp: 6.367 ± 0.71
4.089LeuGlu: 4.089 ± 0.419
2.174LeuPhe: 2.174 ± 0.31
6.937LeuGly: 6.937 ± 1.142
1.708LeuHis: 1.708 ± 0.304
3.158LeuIle: 3.158 ± 0.434
1.294LeuLys: 1.294 ± 0.207
5.798LeuLeu: 5.798 ± 0.559
1.449LeuMet: 1.449 ± 0.228
1.656LeuAsn: 1.656 ± 0.245
4.193LeuPro: 4.193 ± 0.38
2.329LeuGln: 2.329 ± 0.577
6.626LeuArg: 6.626 ± 0.607
4.038LeuSer: 4.038 ± 0.435
7.402LeuThr: 7.402 ± 0.603
5.849LeuVal: 5.849 ± 0.58
1.656LeuTrp: 1.656 ± 0.268
1.346LeuTyr: 1.346 ± 0.245
0.0LeuXaa: 0.0 ± 0.0
Met
2.329MetAla: 2.329 ± 0.305
0.207MetCys: 0.207 ± 0.106
1.139MetAsp: 1.139 ± 0.212
0.776MetGlu: 0.776 ± 0.23
0.518MetPhe: 0.518 ± 0.18
1.398MetGly: 1.398 ± 0.23
0.414MetHis: 0.414 ± 0.156
0.932MetIle: 0.932 ± 0.219
0.621MetLys: 0.621 ± 0.177
1.035MetLeu: 1.035 ± 0.229
0.414MetMet: 0.414 ± 0.128
0.673MetAsn: 0.673 ± 0.22
1.864MetPro: 1.864 ± 0.284
0.828MetGln: 0.828 ± 0.221
1.294MetArg: 1.294 ± 0.24
1.605MetSer: 1.605 ± 0.228
2.692MetThr: 2.692 ± 0.352
1.242MetVal: 1.242 ± 0.288
0.518MetTrp: 0.518 ± 0.172
0.518MetTyr: 0.518 ± 0.159
0.0MetXaa: 0.0 ± 0.0
Asn
4.297AsnAla: 4.297 ± 0.455
0.207AsnCys: 0.207 ± 0.111
1.76AsnAsp: 1.76 ± 0.326
1.294AsnGlu: 1.294 ± 0.238
0.569AsnPhe: 0.569 ± 0.196
2.485AsnGly: 2.485 ± 0.356
0.776AsnHis: 0.776 ± 0.288
1.035AsnIle: 1.035 ± 0.28
0.518AsnLys: 0.518 ± 0.148
2.64AsnLeu: 2.64 ± 0.386
0.362AsnMet: 0.362 ± 0.155
0.725AsnAsn: 0.725 ± 0.197
3.054AsnPro: 3.054 ± 0.373
1.191AsnGln: 1.191 ± 0.263
2.122AsnArg: 2.122 ± 0.248
1.242AsnSer: 1.242 ± 0.317
2.071AsnThr: 2.071 ± 0.348
1.864AsnVal: 1.864 ± 0.306
0.466AsnTrp: 0.466 ± 0.147
0.621AsnTyr: 0.621 ± 0.18
0.0AsnXaa: 0.0 ± 0.0
Pro
8.489ProAla: 8.489 ± 0.768
0.466ProCys: 0.466 ± 0.182
5.694ProAsp: 5.694 ± 0.711
3.831ProGlu: 3.831 ± 0.787
1.501ProPhe: 1.501 ± 0.326
5.021ProGly: 5.021 ± 0.556
1.656ProHis: 1.656 ± 0.338
3.209ProIle: 3.209 ± 0.375
1.242ProLys: 1.242 ± 0.236
3.882ProLeu: 3.882 ± 0.438
0.984ProMet: 0.984 ± 0.178
1.967ProAsn: 1.967 ± 0.333
3.934ProPro: 3.934 ± 0.651
1.76ProGln: 1.76 ± 0.291
4.452ProArg: 4.452 ± 0.519
3.572ProSer: 3.572 ± 0.403
4.555ProThr: 4.555 ± 0.528
4.814ProVal: 4.814 ± 0.534
1.242ProTrp: 1.242 ± 0.312
1.035ProTyr: 1.035 ± 0.218
0.0ProXaa: 0.0 ± 0.0
Gln
4.711GlnAla: 4.711 ± 0.482
0.311GlnCys: 0.311 ± 0.12
1.864GlnAsp: 1.864 ± 0.308
1.915GlnGlu: 1.915 ± 0.272
1.035GlnPhe: 1.035 ± 0.218
1.967GlnGly: 1.967 ± 0.35
1.242GlnHis: 1.242 ± 0.227
1.708GlnIle: 1.708 ± 0.281
0.932GlnLys: 0.932 ± 0.239
3.002GlnLeu: 3.002 ± 0.395
1.035GlnMet: 1.035 ± 0.21
0.88GlnAsn: 0.88 ± 0.192
2.122GlnPro: 2.122 ± 0.364
1.553GlnGln: 1.553 ± 0.254
2.64GlnArg: 2.64 ± 0.319
1.553GlnSer: 1.553 ± 0.334
1.449GlnThr: 1.449 ± 0.308
2.433GlnVal: 2.433 ± 0.415
1.035GlnTrp: 1.035 ± 0.268
0.673GlnTyr: 0.673 ± 0.196
0.0GlnXaa: 0.0 ± 0.0
Arg
9.369ArgAla: 9.369 ± 0.75
1.087ArgCys: 1.087 ± 0.277
6.005ArgAsp: 6.005 ± 0.684
5.177ArgGlu: 5.177 ± 0.65
1.708ArgPhe: 1.708 ± 0.337
5.798ArgGly: 5.798 ± 0.508
1.605ArgHis: 1.605 ± 0.279
3.261ArgIle: 3.261 ± 0.458
3.209ArgLys: 3.209 ± 0.415
5.073ArgLeu: 5.073 ± 0.551
2.433ArgMet: 2.433 ± 0.319
2.174ArgAsn: 2.174 ± 0.363
3.831ArgPro: 3.831 ± 0.467
2.744ArgGln: 2.744 ± 0.404
7.92ArgArg: 7.92 ± 0.851
3.624ArgSer: 3.624 ± 0.381
3.727ArgThr: 3.727 ± 0.488
5.021ArgVal: 5.021 ± 0.462
2.485ArgTrp: 2.485 ± 0.374
1.708ArgTyr: 1.708 ± 0.291
0.0ArgXaa: 0.0 ± 0.0
Ser
6.419SerAla: 6.419 ± 0.662
0.311SerCys: 0.311 ± 0.135
3.934SerAsp: 3.934 ± 0.395
2.122SerGlu: 2.122 ± 0.349
1.346SerPhe: 1.346 ± 0.296
4.918SerGly: 4.918 ± 0.578
0.569SerHis: 0.569 ± 0.184
2.329SerIle: 2.329 ± 0.36
1.449SerLys: 1.449 ± 0.348
4.245SerLeu: 4.245 ± 0.431
1.191SerMet: 1.191 ± 0.208
1.294SerAsn: 1.294 ± 0.258
3.106SerPro: 3.106 ± 0.38
1.035SerGln: 1.035 ± 0.21
3.313SerArg: 3.313 ± 0.412
2.588SerSer: 2.588 ± 0.388
3.882SerThr: 3.882 ± 0.388
3.313SerVal: 3.313 ± 0.423
1.449SerTrp: 1.449 ± 0.278
1.398SerTyr: 1.398 ± 0.239
0.0SerXaa: 0.0 ± 0.0
Thr
7.454ThrAla: 7.454 ± 0.605
0.466ThrCys: 0.466 ± 0.161
4.866ThrAsp: 4.866 ± 0.457
4.348ThrGlu: 4.348 ± 0.48
1.76ThrPhe: 1.76 ± 0.3
6.367ThrGly: 6.367 ± 0.462
0.984ThrHis: 0.984 ± 0.211
3.365ThrIle: 3.365 ± 0.366
2.329ThrLys: 2.329 ± 0.364
4.711ThrLeu: 4.711 ± 0.535
1.656ThrMet: 1.656 ± 0.357
1.605ThrAsn: 1.605 ± 0.225
5.073ThrPro: 5.073 ± 0.549
1.553ThrGln: 1.553 ± 0.249
4.297ThrArg: 4.297 ± 0.51
3.779ThrSer: 3.779 ± 0.496
5.487ThrThr: 5.487 ± 0.713
5.073ThrVal: 5.073 ± 0.545
1.398ThrTrp: 1.398 ± 0.256
1.553ThrTyr: 1.553 ± 0.353
0.0ThrXaa: 0.0 ± 0.0
Val
8.645ValAla: 8.645 ± 0.813
0.932ValCys: 0.932 ± 0.241
5.021ValAsp: 5.021 ± 0.45
4.089ValGlu: 4.089 ± 0.382
1.656ValPhe: 1.656 ± 0.385
5.228ValGly: 5.228 ± 0.671
1.398ValHis: 1.398 ± 0.274
2.899ValIle: 2.899 ± 0.398
2.071ValLys: 2.071 ± 0.297
6.522ValLeu: 6.522 ± 0.582
1.346ValMet: 1.346 ± 0.256
2.485ValAsn: 2.485 ± 0.328
4.504ValPro: 4.504 ± 0.591
2.485ValGln: 2.485 ± 0.35
5.487ValArg: 5.487 ± 0.53
4.555ValSer: 4.555 ± 0.514
5.228ValThr: 5.228 ± 0.607
5.332ValVal: 5.332 ± 0.519
2.071ValTrp: 2.071 ± 0.331
1.139ValTyr: 1.139 ± 0.26
0.0ValXaa: 0.0 ± 0.0
Trp
2.744TrpAla: 2.744 ± 0.393
0.259TrpCys: 0.259 ± 0.12
1.398TrpAsp: 1.398 ± 0.235
1.035TrpGlu: 1.035 ± 0.199
0.673TrpPhe: 0.673 ± 0.224
1.605TrpGly: 1.605 ± 0.259
0.414TrpHis: 0.414 ± 0.133
0.88TrpIle: 0.88 ± 0.214
0.518TrpLys: 0.518 ± 0.16
3.054TrpLeu: 3.054 ± 0.422
0.621TrpMet: 0.621 ± 0.167
0.932TrpAsn: 0.932 ± 0.254
1.294TrpPro: 1.294 ± 0.29
0.88TrpGln: 0.88 ± 0.243
1.398TrpArg: 1.398 ± 0.295
1.449TrpSer: 1.449 ± 0.323
1.864TrpThr: 1.864 ± 0.309
1.398TrpVal: 1.398 ± 0.276
0.828TrpTrp: 0.828 ± 0.188
0.466TrpTyr: 0.466 ± 0.162
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.864TyrAla: 1.864 ± 0.408
0.259TyrCys: 0.259 ± 0.113
1.501TyrAsp: 1.501 ± 0.263
1.139TyrGlu: 1.139 ± 0.272
0.776TyrPhe: 0.776 ± 0.209
1.864TyrGly: 1.864 ± 0.346
0.414TyrHis: 0.414 ± 0.135
0.466TyrIle: 0.466 ± 0.208
0.414TyrLys: 0.414 ± 0.135
1.656TyrLeu: 1.656 ± 0.285
0.207TyrMet: 0.207 ± 0.086
0.828TyrAsn: 0.828 ± 0.231
1.449TyrPro: 1.449 ± 0.331
0.984TyrGln: 0.984 ± 0.232
1.812TyrArg: 1.812 ± 0.369
0.518TyrSer: 0.518 ± 0.139
1.191TyrThr: 1.191 ± 0.291
1.449TyrVal: 1.449 ± 0.304
0.466TyrTrp: 0.466 ± 0.14
0.311TyrTyr: 0.311 ± 0.122
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (19319 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski