Amino acid dipepetide frequency for Gordonia phage Sekhmet

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.114AlaAla: 17.114 ± 1.516
1.033AlaCys: 1.033 ± 0.296
7.621AlaAsp: 7.621 ± 0.496
8.331AlaGlu: 8.331 ± 0.758
3.035AlaPhe: 3.035 ± 0.567
8.719AlaGly: 8.719 ± 0.78
2.067AlaHis: 2.067 ± 0.509
5.167AlaIle: 5.167 ± 0.673
3.875AlaLys: 3.875 ± 0.526
8.912AlaLeu: 8.912 ± 0.75
3.617AlaMet: 3.617 ± 0.638
3.552AlaAsn: 3.552 ± 0.59
5.037AlaPro: 5.037 ± 0.68
5.167AlaGln: 5.167 ± 0.815
8.137AlaArg: 8.137 ± 0.673
6.587AlaSer: 6.587 ± 0.907
7.621AlaThr: 7.621 ± 0.773
8.525AlaVal: 8.525 ± 1.235
2.196AlaTrp: 2.196 ± 0.405
2.519AlaTyr: 2.519 ± 0.29
0.0AlaXaa: 0.0 ± 0.0
Cys
0.517CysAla: 0.517 ± 0.169
0.258CysCys: 0.258 ± 0.161
0.84CysAsp: 0.84 ± 0.265
0.71CysGlu: 0.71 ± 0.249
0.194CysPhe: 0.194 ± 0.111
1.615CysGly: 1.615 ± 0.403
0.387CysHis: 0.387 ± 0.177
0.129CysIle: 0.129 ± 0.076
0.129CysLys: 0.129 ± 0.07
0.387CysLeu: 0.387 ± 0.155
0.129CysMet: 0.129 ± 0.098
0.387CysAsn: 0.387 ± 0.16
0.387CysPro: 0.387 ± 0.174
0.194CysGln: 0.194 ± 0.107
0.904CysArg: 0.904 ± 0.349
0.387CysSer: 0.387 ± 0.144
0.581CysThr: 0.581 ± 0.267
0.581CysVal: 0.581 ± 0.193
0.323CysTrp: 0.323 ± 0.201
0.323CysTyr: 0.323 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
6.781AspAla: 6.781 ± 0.683
0.194AspCys: 0.194 ± 0.107
6.523AspAsp: 6.523 ± 0.824
4.908AspGlu: 4.908 ± 0.849
1.292AspPhe: 1.292 ± 0.305
5.748AspGly: 5.748 ± 0.78
2.325AspHis: 2.325 ± 0.473
2.519AspIle: 2.519 ± 0.462
1.873AspLys: 1.873 ± 0.244
7.104AspLeu: 7.104 ± 0.569
1.162AspMet: 1.162 ± 0.263
1.227AspAsn: 1.227 ± 0.288
5.425AspPro: 5.425 ± 0.555
2.906AspGln: 2.906 ± 0.426
4.65AspArg: 4.65 ± 0.694
2.067AspSer: 2.067 ± 0.318
3.94AspThr: 3.94 ± 0.502
4.521AspVal: 4.521 ± 0.562
1.356AspTrp: 1.356 ± 0.244
1.227AspTyr: 1.227 ± 0.263
0.0AspXaa: 0.0 ± 0.0
Glu
7.75GluAla: 7.75 ± 0.643
1.033GluCys: 1.033 ± 0.277
2.519GluAsp: 2.519 ± 0.429
2.454GluGlu: 2.454 ± 0.371
2.325GluPhe: 2.325 ± 0.415
3.1GluGly: 3.1 ± 0.478
1.033GluHis: 1.033 ± 0.277
2.583GluIle: 2.583 ± 0.549
1.873GluLys: 1.873 ± 0.341
5.554GluLeu: 5.554 ± 0.491
1.356GluMet: 1.356 ± 0.325
1.227GluAsn: 1.227 ± 0.315
2.648GluPro: 2.648 ± 0.411
3.294GluGln: 3.294 ± 0.487
4.908GluArg: 4.908 ± 0.661
3.294GluSer: 3.294 ± 0.497
3.875GluThr: 3.875 ± 0.655
4.004GluVal: 4.004 ± 0.628
1.098GluTrp: 1.098 ± 0.307
2.26GluTyr: 2.26 ± 0.478
0.0GluXaa: 0.0 ± 0.0
Phe
2.39PheAla: 2.39 ± 0.439
0.065PheCys: 0.065 ± 0.063
2.131PheAsp: 2.131 ± 0.366
1.55PheGlu: 1.55 ± 0.285
0.71PhePhe: 0.71 ± 0.252
2.131PheGly: 2.131 ± 0.357
0.581PheHis: 0.581 ± 0.201
1.033PheIle: 1.033 ± 0.271
0.452PheLys: 0.452 ± 0.181
1.615PheLeu: 1.615 ± 0.312
0.452PheMet: 0.452 ± 0.13
0.775PheAsn: 0.775 ± 0.271
1.292PhePro: 1.292 ± 0.299
0.84PheGln: 0.84 ± 0.207
1.356PheArg: 1.356 ± 0.315
1.098PheSer: 1.098 ± 0.209
2.325PheThr: 2.325 ± 0.343
1.937PheVal: 1.937 ± 0.483
0.646PheTrp: 0.646 ± 0.219
0.452PheTyr: 0.452 ± 0.159
0.0PheXaa: 0.0 ± 0.0
Gly
8.202GlyAla: 8.202 ± 0.914
0.84GlyCys: 0.84 ± 0.362
5.167GlyAsp: 5.167 ± 0.471
4.456GlyGlu: 4.456 ± 0.384
2.067GlyPhe: 2.067 ± 0.296
7.879GlyGly: 7.879 ± 1.064
1.679GlyHis: 1.679 ± 0.381
4.198GlyIle: 4.198 ± 0.521
3.035GlyLys: 3.035 ± 0.476
6.458GlyLeu: 6.458 ± 0.623
1.679GlyMet: 1.679 ± 0.362
2.842GlyAsn: 2.842 ± 0.386
3.746GlyPro: 3.746 ± 0.524
2.842GlyGln: 2.842 ± 0.645
6.006GlyArg: 6.006 ± 0.601
4.973GlySer: 4.973 ± 0.757
5.683GlyThr: 5.683 ± 0.71
6.975GlyVal: 6.975 ± 0.757
1.227GlyTrp: 1.227 ± 0.261
2.325GlyTyr: 2.325 ± 0.407
0.0GlyXaa: 0.0 ± 0.0
His
2.131HisAla: 2.131 ± 0.401
0.065HisCys: 0.065 ± 0.066
1.485HisAsp: 1.485 ± 0.392
1.033HisGlu: 1.033 ± 0.287
0.258HisPhe: 0.258 ± 0.141
1.873HisGly: 1.873 ± 0.453
0.84HisHis: 0.84 ± 0.274
1.227HisIle: 1.227 ± 0.384
0.387HisLys: 0.387 ± 0.168
1.679HisLeu: 1.679 ± 0.399
0.517HisMet: 0.517 ± 0.148
0.71HisAsn: 0.71 ± 0.26
1.679HisPro: 1.679 ± 0.335
0.904HisGln: 0.904 ± 0.205
1.55HisArg: 1.55 ± 0.344
0.387HisSer: 0.387 ± 0.131
1.873HisThr: 1.873 ± 0.458
1.356HisVal: 1.356 ± 0.256
0.581HisTrp: 0.581 ± 0.166
0.646HisTyr: 0.646 ± 0.278
0.0HisXaa: 0.0 ± 0.0
Ile
6.458IleAla: 6.458 ± 0.692
0.387IleCys: 0.387 ± 0.177
4.456IleAsp: 4.456 ± 0.575
2.777IleGlu: 2.777 ± 0.464
0.581IlePhe: 0.581 ± 0.251
3.875IleGly: 3.875 ± 0.648
1.098IleHis: 1.098 ± 0.215
1.421IleIle: 1.421 ± 0.276
1.033IleLys: 1.033 ± 0.316
2.002IleLeu: 2.002 ± 0.334
0.904IleMet: 0.904 ± 0.236
1.55IleAsn: 1.55 ± 0.347
3.1IlePro: 3.1 ± 0.364
1.808IleGln: 1.808 ± 0.281
3.229IleArg: 3.229 ± 0.426
2.325IleSer: 2.325 ± 0.352
3.94IleThr: 3.94 ± 0.623
2.842IleVal: 2.842 ± 0.441
0.517IleTrp: 0.517 ± 0.169
1.679IleTyr: 1.679 ± 0.346
0.0IleXaa: 0.0 ± 0.0
Lys
4.069LysAla: 4.069 ± 0.427
0.129LysCys: 0.129 ± 0.098
1.162LysAsp: 1.162 ± 0.3
1.808LysGlu: 1.808 ± 0.35
0.646LysPhe: 0.646 ± 0.227
2.131LysGly: 2.131 ± 0.401
0.775LysHis: 0.775 ± 0.188
1.227LysIle: 1.227 ± 0.244
1.421LysLys: 1.421 ± 0.307
2.971LysLeu: 2.971 ± 0.459
0.581LysMet: 0.581 ± 0.216
1.227LysAsn: 1.227 ± 0.266
2.454LysPro: 2.454 ± 0.478
1.421LysGln: 1.421 ± 0.536
2.26LysArg: 2.26 ± 0.373
1.679LysSer: 1.679 ± 0.44
2.325LysThr: 2.325 ± 0.491
2.067LysVal: 2.067 ± 0.397
0.71LysTrp: 0.71 ± 0.253
0.646LysTyr: 0.646 ± 0.195
0.0LysXaa: 0.0 ± 0.0
Leu
9.946LeuAla: 9.946 ± 1.098
0.646LeuCys: 0.646 ± 0.223
5.619LeuAsp: 5.619 ± 0.799
4.521LeuGlu: 4.521 ± 0.523
2.39LeuPhe: 2.39 ± 0.532
6.523LeuGly: 6.523 ± 0.615
1.292LeuHis: 1.292 ± 0.338
3.423LeuIle: 3.423 ± 0.535
2.26LeuLys: 2.26 ± 0.353
5.49LeuLeu: 5.49 ± 0.614
1.744LeuMet: 1.744 ± 0.301
2.648LeuAsn: 2.648 ± 0.397
4.392LeuPro: 4.392 ± 0.594
2.325LeuGln: 2.325 ± 0.296
6.717LeuArg: 6.717 ± 0.555
4.133LeuSer: 4.133 ± 0.542
6.394LeuThr: 6.394 ± 0.862
5.425LeuVal: 5.425 ± 0.663
0.969LeuTrp: 0.969 ± 0.252
1.615LeuTyr: 1.615 ± 0.276
0.0LeuXaa: 0.0 ± 0.0
Met
2.971MetAla: 2.971 ± 0.772
0.323MetCys: 0.323 ± 0.19
0.969MetAsp: 0.969 ± 0.31
0.387MetGlu: 0.387 ± 0.17
0.452MetPhe: 0.452 ± 0.148
1.55MetGly: 1.55 ± 0.401
0.581MetHis: 0.581 ± 0.182
0.452MetIle: 0.452 ± 0.186
0.581MetLys: 0.581 ± 0.222
1.356MetLeu: 1.356 ± 0.278
0.194MetMet: 0.194 ± 0.096
0.969MetAsn: 0.969 ± 0.218
1.873MetPro: 1.873 ± 0.333
0.904MetGln: 0.904 ± 0.273
1.744MetArg: 1.744 ± 0.305
1.162MetSer: 1.162 ± 0.278
3.165MetThr: 3.165 ± 0.401
1.098MetVal: 1.098 ± 0.223
0.581MetTrp: 0.581 ± 0.249
0.065MetTyr: 0.065 ± 0.066
0.0MetXaa: 0.0 ± 0.0
Asn
2.712AsnAla: 2.712 ± 0.654
0.129AsnCys: 0.129 ± 0.09
2.002AsnAsp: 2.002 ± 0.413
1.679AsnGlu: 1.679 ± 0.293
0.387AsnPhe: 0.387 ± 0.136
2.325AsnGly: 2.325 ± 0.499
0.84AsnHis: 0.84 ± 0.225
1.421AsnIle: 1.421 ± 0.282
1.292AsnLys: 1.292 ± 0.372
2.325AsnLeu: 2.325 ± 0.346
0.323AsnMet: 0.323 ± 0.125
1.033AsnAsn: 1.033 ± 0.254
2.454AsnPro: 2.454 ± 0.38
1.162AsnGln: 1.162 ± 0.302
2.906AsnArg: 2.906 ± 0.454
1.937AsnSer: 1.937 ± 0.295
2.519AsnThr: 2.519 ± 0.451
1.55AsnVal: 1.55 ± 0.337
0.517AsnTrp: 0.517 ± 0.153
0.775AsnTyr: 0.775 ± 0.237
0.0AsnXaa: 0.0 ± 0.0
Pro
6.523ProAla: 6.523 ± 0.61
0.904ProCys: 0.904 ± 0.32
4.456ProAsp: 4.456 ± 0.953
3.81ProGlu: 3.81 ± 0.467
1.162ProPhe: 1.162 ± 0.254
5.942ProGly: 5.942 ± 0.646
0.969ProHis: 0.969 ± 0.264
2.519ProIle: 2.519 ± 0.416
1.421ProLys: 1.421 ± 0.277
3.681ProLeu: 3.681 ± 0.513
1.098ProMet: 1.098 ± 0.212
2.131ProAsn: 2.131 ± 0.379
2.583ProPro: 2.583 ± 0.447
1.421ProGln: 1.421 ± 0.251
3.746ProArg: 3.746 ± 0.647
3.617ProSer: 3.617 ± 0.477
4.585ProThr: 4.585 ± 0.555
4.844ProVal: 4.844 ± 0.528
1.292ProTrp: 1.292 ± 0.284
0.646ProTyr: 0.646 ± 0.187
0.0ProXaa: 0.0 ± 0.0
Gln
4.844GlnAla: 4.844 ± 0.886
0.194GlnCys: 0.194 ± 0.116
1.485GlnAsp: 1.485 ± 0.321
1.679GlnGlu: 1.679 ± 0.325
0.775GlnPhe: 0.775 ± 0.194
2.067GlnGly: 2.067 ± 0.522
1.033GlnHis: 1.033 ± 0.288
2.39GlnIle: 2.39 ± 0.411
1.356GlnLys: 1.356 ± 0.298
3.617GlnLeu: 3.617 ± 0.391
1.227GlnMet: 1.227 ± 0.253
0.581GlnAsn: 0.581 ± 0.274
2.26GlnPro: 2.26 ± 0.35
1.55GlnGln: 1.55 ± 0.441
4.262GlnArg: 4.262 ± 0.607
1.55GlnSer: 1.55 ± 0.387
2.519GlnThr: 2.519 ± 0.534
3.165GlnVal: 3.165 ± 0.458
0.775GlnTrp: 0.775 ± 0.197
0.646GlnTyr: 0.646 ± 0.224
0.0GlnXaa: 0.0 ± 0.0
Arg
8.525ArgAla: 8.525 ± 0.74
0.84ArgCys: 0.84 ± 0.315
4.585ArgAsp: 4.585 ± 0.569
4.456ArgGlu: 4.456 ± 0.522
1.679ArgPhe: 1.679 ± 0.352
4.65ArgGly: 4.65 ± 0.489
1.421ArgHis: 1.421 ± 0.343
3.94ArgIle: 3.94 ± 0.57
3.1ArgLys: 3.1 ± 0.393
6.91ArgLeu: 6.91 ± 0.561
1.615ArgMet: 1.615 ± 0.368
2.648ArgAsn: 2.648 ± 0.402
4.133ArgPro: 4.133 ± 0.665
2.971ArgGln: 2.971 ± 0.511
5.942ArgArg: 5.942 ± 0.924
3.423ArgSer: 3.423 ± 0.478
4.65ArgThr: 4.65 ± 0.654
5.296ArgVal: 5.296 ± 0.499
2.325ArgTrp: 2.325 ± 0.454
2.002ArgTyr: 2.002 ± 0.369
0.0ArgXaa: 0.0 ± 0.0
Ser
5.877SerAla: 5.877 ± 0.625
0.194SerCys: 0.194 ± 0.109
3.423SerAsp: 3.423 ± 0.537
2.39SerGlu: 2.39 ± 0.394
1.292SerPhe: 1.292 ± 0.339
5.619SerGly: 5.619 ± 0.839
0.775SerHis: 0.775 ± 0.243
3.1SerIle: 3.1 ± 0.385
1.679SerLys: 1.679 ± 0.326
4.198SerLeu: 4.198 ± 0.466
1.485SerMet: 1.485 ± 0.343
1.615SerAsn: 1.615 ± 0.402
2.583SerPro: 2.583 ± 0.381
2.454SerGln: 2.454 ± 0.6
2.906SerArg: 2.906 ± 0.521
2.906SerSer: 2.906 ± 0.494
3.875SerThr: 3.875 ± 0.515
3.617SerVal: 3.617 ± 0.459
1.356SerTrp: 1.356 ± 0.249
1.421SerTyr: 1.421 ± 0.37
0.0SerXaa: 0.0 ± 0.0
Thr
9.623ThrAla: 9.623 ± 0.786
0.969ThrCys: 0.969 ± 0.235
4.844ThrAsp: 4.844 ± 0.526
3.294ThrGlu: 3.294 ± 0.438
1.937ThrPhe: 1.937 ± 0.411
7.169ThrGly: 7.169 ± 0.766
1.033ThrHis: 1.033 ± 0.285
4.585ThrIle: 4.585 ± 0.516
2.39ThrLys: 2.39 ± 0.281
5.942ThrLeu: 5.942 ± 0.647
1.033ThrMet: 1.033 ± 0.258
1.421ThrAsn: 1.421 ± 0.322
4.715ThrPro: 4.715 ± 0.646
1.808ThrGln: 1.808 ± 0.417
5.36ThrArg: 5.36 ± 0.823
3.617ThrSer: 3.617 ± 0.503
5.942ThrThr: 5.942 ± 0.812
5.683ThrVal: 5.683 ± 0.577
1.227ThrTrp: 1.227 ± 0.302
1.615ThrTyr: 1.615 ± 0.294
0.0ThrXaa: 0.0 ± 0.0
Val
7.75ValAla: 7.75 ± 0.65
0.581ValCys: 0.581 ± 0.191
5.683ValAsp: 5.683 ± 0.535
5.554ValGlu: 5.554 ± 0.457
1.485ValPhe: 1.485 ± 0.283
6.006ValGly: 6.006 ± 0.729
1.162ValHis: 1.162 ± 0.281
3.681ValIle: 3.681 ± 0.52
2.519ValLys: 2.519 ± 0.471
4.715ValLeu: 4.715 ± 0.514
1.227ValMet: 1.227 ± 0.248
2.519ValAsn: 2.519 ± 0.357
4.069ValPro: 4.069 ± 0.502
2.26ValGln: 2.26 ± 0.533
4.715ValArg: 4.715 ± 0.472
4.779ValSer: 4.779 ± 0.558
5.425ValThr: 5.425 ± 0.559
4.844ValVal: 4.844 ± 0.638
0.71ValTrp: 0.71 ± 0.197
1.421ValTyr: 1.421 ± 0.315
0.0ValXaa: 0.0 ± 0.0
Trp
1.55TrpAla: 1.55 ± 0.29
0.323TrpCys: 0.323 ± 0.147
1.421TrpAsp: 1.421 ± 0.321
1.033TrpGlu: 1.033 ± 0.298
0.646TrpPhe: 0.646 ± 0.171
1.162TrpGly: 1.162 ± 0.286
0.646TrpHis: 0.646 ± 0.247
0.646TrpIle: 0.646 ± 0.21
0.452TrpLys: 0.452 ± 0.175
1.744TrpLeu: 1.744 ± 0.362
0.581TrpMet: 0.581 ± 0.209
0.517TrpAsn: 0.517 ± 0.157
1.098TrpPro: 1.098 ± 0.247
0.517TrpGln: 0.517 ± 0.159
2.131TrpArg: 2.131 ± 0.365
0.969TrpSer: 0.969 ± 0.251
1.679TrpThr: 1.679 ± 0.31
1.098TrpVal: 1.098 ± 0.228
0.517TrpTrp: 0.517 ± 0.168
0.387TrpTyr: 0.387 ± 0.174
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.1TyrAla: 3.1 ± 0.458
0.258TyrCys: 0.258 ± 0.137
1.356TyrAsp: 1.356 ± 0.247
1.356TyrGlu: 1.356 ± 0.276
0.517TyrPhe: 0.517 ± 0.145
2.196TyrGly: 2.196 ± 0.398
0.646TyrHis: 0.646 ± 0.288
0.323TyrIle: 0.323 ± 0.138
0.581TyrLys: 0.581 ± 0.218
1.808TyrLeu: 1.808 ± 0.384
0.387TyrMet: 0.387 ± 0.147
0.71TyrAsn: 0.71 ± 0.247
1.421TyrPro: 1.421 ± 0.287
1.227TyrGln: 1.227 ± 0.29
1.679TyrArg: 1.679 ± 0.289
1.873TyrSer: 1.873 ± 0.302
1.356TyrThr: 1.356 ± 0.266
1.679TyrVal: 1.679 ± 0.253
0.194TyrTrp: 0.194 ± 0.134
0.452TyrTyr: 0.452 ± 0.129
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (15485 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski