Salmonella phage vB_SalS_SA001
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 157 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D3QJ49|A0A7D3QJ49_9CAUD Uncharacterized protein OS=Salmonella phage vB_SalS_SA001 OX=2739751 GN=ACSA001_0650 PE=4 SV=1
MM1 pKa = 7.38 SNKK4 pKa = 9.58 IVVTKK9 pKa = 10.11 ISTMVDD15 pKa = 2.72 VFYY18 pKa = 11.54 VPDD21 pKa = 3.75 TPEE24 pKa = 3.29 NRR26 pKa = 11.84 QAVEE30 pKa = 3.63 RR31 pKa = 11.84 GEE33 pKa = 4.26 YY34 pKa = 10.66 DD35 pKa = 3.62 SVIYY39 pKa = 10.64 DD40 pKa = 3.26 ADD42 pKa = 3.57 GYY44 pKa = 9.51 YY45 pKa = 10.25 QYY47 pKa = 11.53 LVDD50 pKa = 5.15 SYY52 pKa = 11.94 GEE54 pKa = 4.12 EE55 pKa = 3.88 EE56 pKa = 5.01 KK57 pKa = 9.68 ITHH60 pKa = 7.11 RR61 pKa = 11.84 LPDD64 pKa = 3.26
Molecular weight: 7.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.985
IPC2_protein 4.317
IPC_protein 4.19
Toseland 4.012
ProMoST 4.228
Dawson 4.164
Bjellqvist 4.38
Wikipedia 4.075
Rodwell 4.024
Grimsley 3.923
Solomon 4.151
Lehninger 4.113
Nozaki 4.291
DTASelect 4.469
Thurlkill 4.05
EMBOSS 4.088
Sillero 4.317
Patrickios 1.977
IPC_peptide 4.151
IPC2_peptide 4.291
IPC2.peptide.svr19 4.241
Protein with the highest isoelectric point:
>tr|A0A7D3QJJ8|A0A7D3QJJ8_9CAUD Uncharacterized protein OS=Salmonella phage vB_SalS_SA001 OX=2739751 GN=ACSA001_1460 PE=4 SV=1
MM1 pKa = 7.62 LRR3 pKa = 11.84 TLNRR7 pKa = 11.84 KK8 pKa = 8.68 LLFLLEE14 pKa = 4.18 LLKK17 pKa = 10.32 PLPLSPLHH25 pKa = 6.66 LLLNLLHH32 pKa = 6.3 QLLNRR37 pKa = 11.84 RR38 pKa = 11.84 NQQLQLKK45 pKa = 7.64 YY46 pKa = 10.54 LRR48 pKa = 11.84 LMLKK52 pKa = 9.85 LAKK55 pKa = 9.58 IRR57 pKa = 11.84 QRR59 pKa = 11.84 LVKK62 pKa = 10.51 LMQKK66 pKa = 10.19 LVRR69 pKa = 11.84 LTQKK73 pKa = 10.43 LVRR76 pKa = 11.84 LMQRR80 pKa = 11.84 LAKK83 pKa = 9.8 IRR85 pKa = 11.84 QRR87 pKa = 11.84 LVKK90 pKa = 10.7 LMLLLLLLQQRR101 pKa = 11.84 LVKK104 pKa = 10.16 IRR106 pKa = 11.84 QRR108 pKa = 11.84 LVKK111 pKa = 10.7 LMLLLLLRR119 pKa = 11.84 ILKK122 pKa = 10.07 AIEE125 pKa = 3.88 MGQKK129 pKa = 10.33 YY130 pKa = 10.14 SLHH133 pKa = 6.49 KK134 pKa = 10.16 PPHH137 pKa = 4.59 QHH139 pKa = 7.14 PLQKK143 pKa = 9.76 PRR145 pKa = 11.84 KK146 pKa = 8.78 LMQKK150 pKa = 9.73 LVKK153 pKa = 10.17 PMQKK157 pKa = 9.9 LVKK160 pKa = 9.94 PMQKK164 pKa = 9.93 LVRR167 pKa = 11.84 LMQPIPQPQLTNQQ180 pKa = 3.14
Molecular weight: 21.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 10.921
IPC_protein 12.266
Toseland 12.442
ProMoST 12.91
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.34
Grimsley 12.486
Solomon 12.925
Lehninger 12.822
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.062
IPC_peptide 12.925
IPC2_peptide 11.915
IPC2.peptide.svr19 8.972
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
157
0
157
30683
32
1226
195.4
22.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.252 ± 0.375
1.137 ± 0.102
6.108 ± 0.163
7.007 ± 0.216
4.142 ± 0.147
6.284 ± 0.236
1.949 ± 0.122
6.799 ± 0.165
7.346 ± 0.219
8.673 ± 0.292
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.669 ± 0.131
5.234 ± 0.201
3.539 ± 0.127
3.738 ± 0.189
4.68 ± 0.134
6.297 ± 0.199
5.547 ± 0.156
6.407 ± 0.151
1.229 ± 0.086
3.963 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here