Lactobacillus sanfranciscensis (strain TMW 1.1304)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Fructilactobacillus; Fructilactobacillus sanfranciscensis

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2KU24|G2KU24_LACSM Ppx-GppA domain-containing protein OS=Lactobacillus sanfranciscensis (strain TMW 1.1304) OX=714313 GN=LSA_03910 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.27FDD4 pKa = 4.42VIVGNPPYY12 pKa = 10.55QGDD15 pKa = 4.01AKK17 pKa = 10.21QQIYY21 pKa = 10.01TDD23 pKa = 4.79FYY25 pKa = 11.32LSIIGEE31 pKa = 4.22NTPTSTSGGG40 pKa = 3.63

Molecular weight: 4.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2KW38|G2KW38_LACSM 30S ribosomal protein S14 type Z OS=Lactobacillus sanfranciscensis (strain TMW 1.1304) OX=714313 GN=rpsZ PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.61KK9 pKa = 7.34RR10 pKa = 11.84HH11 pKa = 5.74RR12 pKa = 11.84NRR14 pKa = 11.84VHH16 pKa = 6.74GFRR19 pKa = 11.84ARR21 pKa = 11.84MQTKK25 pKa = 9.91GGRR28 pKa = 11.84KK29 pKa = 8.01VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1263

0

1263

369316

35

1435

292.4

32.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.924 ± 0.071

0.513 ± 0.019

5.947 ± 0.074

5.789 ± 0.065

4.507 ± 0.06

6.425 ± 0.067

2.045 ± 0.025

7.978 ± 0.061

7.867 ± 0.071

9.354 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.813 ± 0.035

5.793 ± 0.069

3.459 ± 0.032

4.054 ± 0.056

3.723 ± 0.049

5.87 ± 0.051

5.568 ± 0.041

6.877 ± 0.062

0.904 ± 0.026

3.592 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski