Suhomyces tanzawaensis NRRL Y-17324
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5882 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E4SR02|A0A1E4SR02_9ASCO Coatomer subunit beta' OS=Suhomyces tanzawaensis NRRL Y-17324 OX=984487 GN=CANTADRAFT_46178 PE=3 SV=1
GG1 pKa = 7.44 SYY3 pKa = 10.25 SANLTNEE10 pKa = 3.87 NVFYY14 pKa = 7.85 TTNITVGSEE23 pKa = 3.75 KK24 pKa = 10.65 KK25 pKa = 10.41 QILVDD30 pKa = 4.57 LDD32 pKa = 3.9 TGSSDD37 pKa = 4.18 LWVLSANGTDD47 pKa = 4.24 GFASDD52 pKa = 3.61 TAEE55 pKa = 4.12 LQSNGYY61 pKa = 10.07 LDD63 pKa = 4.46 AEE65 pKa = 4.66 KK66 pKa = 9.99 STSLKK71 pKa = 9.43 TDD73 pKa = 3.03 NNEE76 pKa = 3.7 FFITYY81 pKa = 10.29 GDD83 pKa = 3.3 NTFAYY88 pKa = 9.69 GEE90 pKa = 4.38 YY91 pKa = 9.07 VTDD94 pKa = 5.64 SIAIPNGPSIDD105 pKa = 3.77 VEE107 pKa = 4.29 FGLAFTTVNPFGIFGIGFDD126 pKa = 4.03 LNEE129 pKa = 4.57 AGFSSGAAPIYY140 pKa = 10.49 NNYY143 pKa = 8.91 PATLKK148 pKa = 11.03 KK149 pKa = 10.58 NGLIGKK155 pKa = 8.33 NAYY158 pKa = 9.79 SLYY161 pKa = 10.54 LNSPNATTGSVIFGGYY177 pKa = 7.35 DD178 pKa = 3.19 TKK180 pKa = 11.22 KK181 pKa = 10.29 VDD183 pKa = 3.53 GSFATLQTVNDD194 pKa = 3.56 QVLAINVDD202 pKa = 3.78 SVTVGDD208 pKa = 4.63 NEE210 pKa = 4.06 IQYY213 pKa = 10.51 GSPALLDD220 pKa = 3.59 SGTTITYY227 pKa = 10.05 LPRR230 pKa = 11.84 NVYY233 pKa = 10.1 QGIIQNLPGSNTPYY247 pKa = 10.31 GWRR250 pKa = 11.84 ITTDD254 pKa = 2.88 EE255 pKa = 4.37 AQGHH259 pKa = 7.01 DD260 pKa = 3.08 ITYY263 pKa = 10.16 KK264 pKa = 10.77 FNDD267 pKa = 3.46 VTVKK271 pKa = 10.59 VPVSSLVGPTYY282 pKa = 10.84 DD283 pKa = 4.7 DD284 pKa = 3.1 QGKK287 pKa = 9.25 LLDD290 pKa = 3.93 YY291 pKa = 10.7 TSLQVSIGGSILGDD305 pKa = 3.16 NFLRR309 pKa = 11.84 YY310 pKa = 9.86 AYY312 pKa = 10.56 VIFNLDD318 pKa = 3.39 DD319 pKa = 3.57 QKK321 pKa = 11.53 ISVAQAKK328 pKa = 7.99 FTDD331 pKa = 4.31 DD332 pKa = 4.4 SDD334 pKa = 3.6 IQAII338 pKa = 3.83
Molecular weight: 36.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.91
IPC_protein 3.935
Toseland 3.706
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.897
Rodwell 3.757
Grimsley 3.605
Solomon 3.935
Lehninger 3.884
Nozaki 4.05
DTASelect 4.329
Thurlkill 3.757
EMBOSS 3.897
Sillero 4.062
Patrickios 0.973
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.935
Protein with the highest isoelectric point:
>tr|A0A1E4SH60|A0A1E4SH60_9ASCO DUF676 domain-containing protein OS=Suhomyces tanzawaensis NRRL Y-17324 OX=984487 GN=CANTADRAFT_22712 PE=3 SV=1
MM1 pKa = 7.4 TPATQQWWGSPSPSPRR17 pKa = 11.84 PNRR20 pKa = 11.84 RR21 pKa = 11.84 GARR24 pKa = 11.84 VRR26 pKa = 11.84 WGRR29 pKa = 11.84 TNGSPPAAIPLCSPPPGSKK48 pKa = 10.58 GPPTFRR54 pKa = 11.84 KK55 pKa = 9.56 RR56 pKa = 11.84 QVPLSRR62 pKa = 11.84 GGAKK66 pKa = 9.76 SEE68 pKa = 4.11 PLLSRR73 pKa = 11.84 GGMLRR78 pKa = 11.84 ASPRR82 pKa = 11.84 RR83 pKa = 11.84 LRR85 pKa = 3.78
Molecular weight: 9.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.376
IPC2_protein 10.804
IPC_protein 12.398
Toseland 12.574
ProMoST 13.071
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.042
Rodwell 12.164
Grimsley 12.603
Solomon 13.071
Lehninger 12.969
Nozaki 12.574
DTASelect 12.559
Thurlkill 12.574
EMBOSS 13.071
Sillero 12.574
Patrickios 11.901
IPC_peptide 13.071
IPC2_peptide 12.062
IPC2.peptide.svr19 9.136
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5881
1
5882
2688497
50
4399
457.1
51.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.097 ± 0.034
1.048 ± 0.011
5.844 ± 0.025
6.541 ± 0.04
4.399 ± 0.02
5.425 ± 0.028
2.296 ± 0.014
6.376 ± 0.027
6.925 ± 0.034
9.938 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.803 ± 0.011
5.494 ± 0.028
4.723 ± 0.029
4.031 ± 0.025
4.26 ± 0.022
8.704 ± 0.041
5.595 ± 0.018
6.0 ± 0.021
1.041 ± 0.01
3.454 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here