Prochlorococcus phage P-SSP10
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M1U3M4|M1U3M4_9CAUD Uncharacterized protein OS=Prochlorococcus phage P-SSP10 OX=885867 GN=PROG_00017 PE=4 SV=1
MM1 pKa = 7.75 AGPYY5 pKa = 10.66 VNVEE9 pKa = 3.89 TNANYY14 pKa = 9.36 TGSDD18 pKa = 3.57 YY19 pKa = 11.1 TSRR22 pKa = 11.84 ATDD25 pKa = 2.97 LHH27 pKa = 7.68 IGYY30 pKa = 9.8 EE31 pKa = 4.09 NEE33 pKa = 4.27 LGSLAYY39 pKa = 9.89 YY40 pKa = 9.15 VQGGKK45 pKa = 8.35 TINAADD51 pKa = 4.03 GVDD54 pKa = 3.72 SEE56 pKa = 5.85 SNFSGKK62 pKa = 10.51 LGGNISATDD71 pKa = 3.41 KK72 pKa = 10.96 LGLYY76 pKa = 10.58 GEE78 pKa = 4.7 VSFSQVEE85 pKa = 4.29 DD86 pKa = 3.61 ADD88 pKa = 3.71 NTYY91 pKa = 8.69 GTKK94 pKa = 10.52 LGAKK98 pKa = 9.94 YY99 pKa = 10.77 SFF101 pKa = 4.04
Molecular weight: 10.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.079
IPC2_protein 4.317
IPC_protein 4.19
Toseland 4.012
ProMoST 4.342
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.075
Rodwell 4.024
Grimsley 3.923
Solomon 4.151
Lehninger 4.113
Nozaki 4.291
DTASelect 4.469
Thurlkill 4.05
EMBOSS 4.088
Sillero 4.317
Patrickios 0.604
IPC_peptide 4.151
IPC2_peptide 4.291
IPC2.peptide.svr19 4.207
Protein with the highest isoelectric point:
>tr|M1U3P4|M1U3P4_9CAUD Uncharacterized protein OS=Prochlorococcus phage P-SSP10 OX=885867 GN=PROG_00050 PE=4 SV=1
MM1 pKa = 7.57 APKK4 pKa = 10.32 KK5 pKa = 10.8 LPLSKK10 pKa = 10.36 LKK12 pKa = 10.69 KK13 pKa = 8.83 SARR16 pKa = 11.84 NYY18 pKa = 10.25 RR19 pKa = 11.84 KK20 pKa = 9.55 NPLSRR25 pKa = 11.84 EE26 pKa = 4.27 HH27 pKa = 6.92 KK28 pKa = 10.34 NSEE31 pKa = 3.54 QRR33 pKa = 11.84 KK34 pKa = 8.63 RR35 pKa = 11.84 NKK37 pKa = 10.33 LKK39 pKa = 10.46 INKK42 pKa = 9.45 KK43 pKa = 9.83 YY44 pKa = 10.21 RR45 pKa = 11.84 AEE47 pKa = 4.07 LNSARR52 pKa = 11.84 RR53 pKa = 11.84 KK54 pKa = 9.93 DD55 pKa = 3.56 GNYY58 pKa = 10.37 GKK60 pKa = 10.66 GGKK63 pKa = 9.68 DD64 pKa = 3.2 YY65 pKa = 11.44 SHH67 pKa = 7.1 TKK69 pKa = 10.04 RR70 pKa = 11.84 GTLVRR75 pKa = 11.84 EE76 pKa = 4.14 DD77 pKa = 3.41 ASKK80 pKa = 10.82 NRR82 pKa = 11.84 ARR84 pKa = 11.84 NRR86 pKa = 11.84 SKK88 pKa = 10.92 KK89 pKa = 10.27
Molecular weight: 10.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.231
IPC2_protein 10.233
IPC_protein 11.199
Toseland 11.447
ProMoST 11.579
Dawson 11.477
Bjellqvist 11.286
Wikipedia 11.784
Rodwell 11.725
Grimsley 11.506
Solomon 11.769
Lehninger 11.711
Nozaki 11.418
DTASelect 11.286
Thurlkill 11.433
EMBOSS 11.886
Sillero 11.433
Patrickios 11.447
IPC_peptide 11.784
IPC2_peptide 10.526
IPC2.peptide.svr19 8.485
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
49
0
49
13676
49
1363
279.1
30.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.716 ± 0.445
0.899 ± 0.167
6.617 ± 0.295
6.171 ± 0.35
3.627 ± 0.179
7.349 ± 0.46
1.784 ± 0.163
5.462 ± 0.226
6.53 ± 0.446
7.817 ± 0.374
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.325 ± 0.224
6.003 ± 0.385
3.795 ± 0.301
3.985 ± 0.258
4.27 ± 0.239
6.581 ± 0.328
6.778 ± 0.488
6.12 ± 0.262
1.44 ± 0.145
3.729 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here