Cyanophage Syn10
Average proteome isoelectric point is 5.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 205 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M4SJZ1|M4SJZ1_9CAUD Single-stranded DNA binding protein OS=Cyanophage Syn10 OX=536472 GN=CPUG_00162 PE=4 SV=1
MM1 pKa = 7.77 AKK3 pKa = 9.54 TGLLAQSKK11 pKa = 8.98 PAAATNTVLYY21 pKa = 9.23 KK22 pKa = 10.95 APIGNSASAVLTVANDD38 pKa = 4.12 GTGSAYY44 pKa = 10.88 SLALKK49 pKa = 10.45 DD50 pKa = 3.34 WDD52 pKa = 4.21 QKK54 pKa = 10.53 LTLDD58 pKa = 3.64 ANSYY62 pKa = 10.0 LLHH65 pKa = 6.9 PGDD68 pKa = 4.37 VITAYY73 pKa = 10.57 RR74 pKa = 11.84 IDD76 pKa = 3.74 VDD78 pKa = 3.14 TDD80 pKa = 3.22 MNANSGFTSGLALTSGDD97 pKa = 3.84 EE98 pKa = 4.07 EE99 pKa = 4.69 KK100 pKa = 11.1 VFQFEE105 pKa = 4.37 SFYY108 pKa = 11.47 VPAFTEE114 pKa = 3.99 IFVKK118 pKa = 10.59 DD119 pKa = 3.36 VALRR123 pKa = 11.84 QVTIEE128 pKa = 4.08 SVTGNFEE135 pKa = 3.98 VGQTLTTGTSPDD147 pKa = 3.53 DD148 pKa = 3.56 TEE150 pKa = 4.19 ATVFAVDD157 pKa = 3.44 DD158 pKa = 4.09 TGANTILYY166 pKa = 9.28 IGPSTINGTGAEE178 pKa = 4.23 FTDD181 pKa = 3.92 GDD183 pKa = 3.9 IVATAFGGSATISAGGIAAAVNAFVFSTTTAGGVYY218 pKa = 10.02 DD219 pKa = 3.79 YY220 pKa = 11.0 AQNGLEE226 pKa = 3.96 IFADD230 pKa = 3.44 RR231 pKa = 11.84 AYY233 pKa = 10.78 RR234 pKa = 11.84 FDD236 pKa = 3.86 VSDD239 pKa = 3.41 SSMTGRR245 pKa = 11.84 DD246 pKa = 3.47 FKK248 pKa = 11.5 LSITANGEE256 pKa = 3.74 WGPDD260 pKa = 3.37 GVIGGDD266 pKa = 3.51 PSDD269 pKa = 5.39 DD270 pKa = 3.35 GTEE273 pKa = 3.94 YY274 pKa = 8.06 TTNKK278 pKa = 8.76 TINGTAGSASAYY290 pKa = 8.74 VQYY293 pKa = 11.2 DD294 pKa = 3.45 LAGTGITGSLYY305 pKa = 10.48 FYY307 pKa = 11.02 DD308 pKa = 4.9 GGTGTAGNSIYY319 pKa = 10.87 GGTSRR324 pKa = 11.84 VLSTSNQYY332 pKa = 9.8 TYY334 pKa = 11.03 TGMYY338 pKa = 9.08 VYY340 pKa = 10.58 NVNGDD345 pKa = 3.7 LSGGTVTFTHH355 pKa = 6.75 NDD357 pKa = 2.73 VTYY360 pKa = 9.41 TIDD363 pKa = 3.4 SVTVGAYY370 pKa = 10.05 GYY372 pKa = 9.51 VRR374 pKa = 11.84 SYY376 pKa = 11.17 VGTEE380 pKa = 3.56 LRR382 pKa = 11.84 VCLGAGSTDD391 pKa = 3.64 FAGTNTFRR399 pKa = 11.84 DD400 pKa = 3.69 NPISQTATRR409 pKa = 11.84 NTATVSSVDD418 pKa = 3.39 AGSADD423 pKa = 3.47 VDD425 pKa = 3.69 NEE427 pKa = 4.32 SYY429 pKa = 11.17 LIISKK434 pKa = 9.2 TNAANNIDD442 pKa = 3.94 RR443 pKa = 11.84 TTSLVVGPGEE453 pKa = 4.01 KK454 pKa = 10.33 LIVTSTTQNNVFNLVGFEE472 pKa = 4.24 DD473 pKa = 4.19 VSTSFTTRR481 pKa = 11.84 VNVADD486 pKa = 3.9 YY487 pKa = 10.76 GGAGAGDD494 pKa = 3.92 GGGGVAPP501 pKa = 4.93
Molecular weight: 51.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.91
IPC_protein 3.948
Toseland 3.719
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.897
Rodwell 3.77
Grimsley 3.617
Solomon 3.948
Lehninger 3.897
Nozaki 4.05
DTASelect 4.342
Thurlkill 3.77
EMBOSS 3.897
Sillero 4.075
Patrickios 1.1
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 3.938
Protein with the highest isoelectric point:
>tr|M4SPJ5|M4SPJ5_9CAUD Photosystem II D1 protein OS=Cyanophage Syn10 OX=536472 GN=CPUG_00181 PE=3 SV=1
MM1 pKa = 7.35 MKK3 pKa = 10.43 LPNWQHH9 pKa = 5.58 HH10 pKa = 5.45 SKK12 pKa = 11.03 KK13 pKa = 9.71 EE14 pKa = 3.99 VKK16 pKa = 9.14 RR17 pKa = 11.84 TLKK20 pKa = 10.39 PQALRR25 pKa = 11.84 QAKK28 pKa = 9.23 KK29 pKa = 10.21 RR30 pKa = 11.84 RR31 pKa = 11.84 AALKK35 pKa = 10.43 AKK37 pKa = 10.12 LLAASVVLVGFASPAVAHH55 pKa = 6.55 PRR57 pKa = 11.84 HH58 pKa = 6.02 KK59 pKa = 10.05 HH60 pKa = 4.76 HH61 pKa = 7.33 HH62 pKa = 5.83 DD63 pKa = 3.48 HH64 pKa = 6.25 VEE66 pKa = 3.53 KK67 pKa = 10.36 RR68 pKa = 11.84 YY69 pKa = 7.22 YY70 pKa = 9.1 TCHH73 pKa = 4.97 SHH75 pKa = 6.17 PRR77 pKa = 11.84 KK78 pKa = 10.11 HH79 pKa = 5.86 ITWHH83 pKa = 5.94 CHH85 pKa = 4.54 DD86 pKa = 4.06 HH87 pKa = 7.12 DD88 pKa = 3.62 RR89 pKa = 11.84 WRR91 pKa = 11.84 HH92 pKa = 3.83 GKK94 pKa = 9.45 KK95 pKa = 9.85 RR96 pKa = 11.84 WDD98 pKa = 3.52 YY99 pKa = 10.18 YY100 pKa = 8.86 YY101 pKa = 9.75 PRR103 pKa = 11.84 PIFEE107 pKa = 4.35 FHH109 pKa = 4.86 VHH111 pKa = 5.94
Molecular weight: 13.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.897
IPC_protein 10.394
Toseland 10.804
ProMoST 10.379
Dawson 10.891
Bjellqvist 10.54
Wikipedia 11.038
Rodwell 11.345
Grimsley 10.921
Solomon 10.95
Lehninger 10.935
Nozaki 10.774
DTASelect 10.526
Thurlkill 10.789
EMBOSS 11.184
Sillero 10.804
Patrickios 11.067
IPC_peptide 10.965
IPC2_peptide 9.341
IPC2.peptide.svr19 8.525
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
205
0
205
53933
44
6076
263.1
29.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.771 ± 0.189
0.979 ± 0.091
6.786 ± 0.139
6.067 ± 0.266
4.391 ± 0.113
7.776 ± 0.384
1.667 ± 0.135
6.401 ± 0.21
5.605 ± 0.414
7.088 ± 0.167
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.093 ± 0.2
5.963 ± 0.205
4.075 ± 0.158
3.701 ± 0.094
4.079 ± 0.141
6.823 ± 0.227
7.43 ± 0.407
6.649 ± 0.181
1.239 ± 0.098
4.417 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here