Enterococcus phage LY0322

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Efquatrovirus; Enterococcus virus LY0322

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2S1GSE0|A0A2S1GSE0_9CAUD Uncharacterized protein OS=Enterococcus phage LY0322 OX=2172042 PE=4 SV=1
MM1 pKa = 7.09GRR3 pKa = 11.84EE4 pKa = 4.18ANFSRR9 pKa = 11.84EE10 pKa = 3.77VVKK13 pKa = 10.59YY14 pKa = 9.86LKK16 pKa = 10.72SRR18 pKa = 11.84GALVNVNTANIYY30 pKa = 10.78DD31 pKa = 3.64RR32 pKa = 11.84VGRR35 pKa = 11.84SDD37 pKa = 4.36IEE39 pKa = 3.86ACYY42 pKa = 9.77RR43 pKa = 11.84GYY45 pKa = 10.13WISLEE50 pKa = 4.16LKK52 pKa = 9.56VGNYY56 pKa = 9.49QPDD59 pKa = 3.49QLQIRR64 pKa = 11.84YY65 pKa = 8.3LQEE68 pKa = 3.23VRR70 pKa = 11.84TAGGYY75 pKa = 10.6GILLRR80 pKa = 11.84DD81 pKa = 3.79TLDD84 pKa = 3.92DD85 pKa = 5.3LEE87 pKa = 6.23DD88 pKa = 4.2LLLHH92 pKa = 7.0LDD94 pKa = 3.47QMDD97 pKa = 3.78NGVEE101 pKa = 3.9YY102 pKa = 8.96PYY104 pKa = 10.37TYY106 pKa = 9.72EE107 pKa = 4.68QPDD110 pKa = 4.01LPEE113 pKa = 4.81INYY116 pKa = 10.52DD117 pKa = 3.76EE118 pKa = 6.04LEE120 pKa = 3.93IDD122 pKa = 3.54YY123 pKa = 10.78DD124 pKa = 3.71

Molecular weight:
14.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2S1GSH2|A0A2S1GSH2_9CAUD Uncharacterized protein OS=Enterococcus phage LY0322 OX=2172042 PE=4 SV=1
MM1 pKa = 7.56SKK3 pKa = 10.96LKK5 pKa = 9.69TFKK8 pKa = 9.59VTYY11 pKa = 10.58GNIEE15 pKa = 4.13NKK17 pKa = 8.16VQRR20 pKa = 11.84AYY22 pKa = 11.25LKK24 pKa = 10.09AYY26 pKa = 9.59HH27 pKa = 6.2YY28 pKa = 11.33NEE30 pKa = 3.83ALRR33 pKa = 11.84RR34 pKa = 11.84AKK36 pKa = 10.24RR37 pKa = 11.84INGLYY42 pKa = 10.32CIIKK46 pKa = 10.52LEE48 pKa = 4.15EE49 pKa = 3.71VDD51 pKa = 3.62NASKK55 pKa = 11.44NEE57 pKa = 3.78LLYY60 pKa = 11.17LFNKK64 pKa = 9.69

Molecular weight:
7.63 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12338

47

1456

192.8

21.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.674 ± 0.423

0.689 ± 0.132

6.006 ± 0.277

7.911 ± 0.546

4.247 ± 0.223

6.614 ± 0.645

1.532 ± 0.171

6.922 ± 0.24

8.907 ± 0.292

8.146 ± 0.309

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.885 ± 0.19

6.468 ± 0.289

2.861 ± 0.251

3.801 ± 0.175

3.688 ± 0.22

5.438 ± 0.225

6.136 ± 0.321

6.703 ± 0.205

1.313 ± 0.107

4.061 ± 0.391

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski