Enterococcus phage LY0322
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1GSE0|A0A2S1GSE0_9CAUD Uncharacterized protein OS=Enterococcus phage LY0322 OX=2172042 PE=4 SV=1
MM1 pKa = 7.09 GRR3 pKa = 11.84 EE4 pKa = 4.18 ANFSRR9 pKa = 11.84 EE10 pKa = 3.77 VVKK13 pKa = 10.59 YY14 pKa = 9.86 LKK16 pKa = 10.72 SRR18 pKa = 11.84 GALVNVNTANIYY30 pKa = 10.78 DD31 pKa = 3.64 RR32 pKa = 11.84 VGRR35 pKa = 11.84 SDD37 pKa = 4.36 IEE39 pKa = 3.86 ACYY42 pKa = 9.77 RR43 pKa = 11.84 GYY45 pKa = 10.13 WISLEE50 pKa = 4.16 LKK52 pKa = 9.56 VGNYY56 pKa = 9.49 QPDD59 pKa = 3.49 QLQIRR64 pKa = 11.84 YY65 pKa = 8.3 LQEE68 pKa = 3.23 VRR70 pKa = 11.84 TAGGYY75 pKa = 10.6 GILLRR80 pKa = 11.84 DD81 pKa = 3.79 TLDD84 pKa = 3.92 DD85 pKa = 5.3 LEE87 pKa = 6.23 DD88 pKa = 4.2 LLLHH92 pKa = 7.0 LDD94 pKa = 3.47 QMDD97 pKa = 3.78 NGVEE101 pKa = 3.9 YY102 pKa = 8.96 PYY104 pKa = 10.37 TYY106 pKa = 9.72 EE107 pKa = 4.68 QPDD110 pKa = 4.01 LPEE113 pKa = 4.81 INYY116 pKa = 10.52 DD117 pKa = 3.76 EE118 pKa = 6.04 LEE120 pKa = 3.93 IDD122 pKa = 3.54 YY123 pKa = 10.78 DD124 pKa = 3.71
Molecular weight: 14.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.966
IPC2_protein 4.266
IPC_protein 4.202
Toseland 4.012
ProMoST 4.266
Dawson 4.164
Bjellqvist 4.355
Wikipedia 4.075
Rodwell 4.024
Grimsley 3.923
Solomon 4.164
Lehninger 4.113
Nozaki 4.279
DTASelect 4.469
Thurlkill 4.037
EMBOSS 4.088
Sillero 4.317
Patrickios 1.977
IPC_peptide 4.164
IPC2_peptide 4.291
IPC2.peptide.svr19 4.227
Protein with the highest isoelectric point:
>tr|A0A2S1GSH2|A0A2S1GSH2_9CAUD Uncharacterized protein OS=Enterococcus phage LY0322 OX=2172042 PE=4 SV=1
MM1 pKa = 7.56 SKK3 pKa = 10.96 LKK5 pKa = 9.69 TFKK8 pKa = 9.59 VTYY11 pKa = 10.58 GNIEE15 pKa = 4.13 NKK17 pKa = 8.16 VQRR20 pKa = 11.84 AYY22 pKa = 11.25 LKK24 pKa = 10.09 AYY26 pKa = 9.59 HH27 pKa = 6.2 YY28 pKa = 11.33 NEE30 pKa = 3.83 ALRR33 pKa = 11.84 RR34 pKa = 11.84 AKK36 pKa = 10.24 RR37 pKa = 11.84 INGLYY42 pKa = 10.32 CIIKK46 pKa = 10.52 LEE48 pKa = 4.15 EE49 pKa = 3.71 VDD51 pKa = 3.62 NASKK55 pKa = 11.44 NEE57 pKa = 3.78 LLYY60 pKa = 11.17 LFNKK64 pKa = 9.69
Molecular weight: 7.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.151
IPC2_protein 9.268
IPC_protein 9.209
Toseland 9.765
ProMoST 9.604
Dawson 10.028
Bjellqvist 9.721
Wikipedia 10.204
Rodwell 10.394
Grimsley 10.116
Solomon 10.058
Lehninger 10.028
Nozaki 9.78
DTASelect 9.706
Thurlkill 9.853
EMBOSS 10.175
Sillero 9.94
Patrickios 7.556
IPC_peptide 10.058
IPC2_peptide 8.346
IPC2.peptide.svr19 8.273
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
64
0
64
12338
47
1456
192.8
21.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.674 ± 0.423
0.689 ± 0.132
6.006 ± 0.277
7.911 ± 0.546
4.247 ± 0.223
6.614 ± 0.645
1.532 ± 0.171
6.922 ± 0.24
8.907 ± 0.292
8.146 ± 0.309
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.885 ± 0.19
6.468 ± 0.289
2.861 ± 0.251
3.801 ± 0.175
3.688 ± 0.22
5.438 ± 0.225
6.136 ± 0.321
6.703 ± 0.205
1.313 ± 0.107
4.061 ± 0.391
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here