Mycobacterium pseudokansasii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5730 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A498QZP5|A0A498QZP5_9MYCO Non-specific serine/threonine protein kinase OS=Mycobacterium pseudokansasii OX=2341080 GN=pknJ_2 PE=4 SV=1
MM1 pKa = 7.23NFSVSPPEE9 pKa = 3.89INSARR14 pKa = 11.84IFSGAGAGPMLAAAAAWDD32 pKa = 3.77QLAGEE37 pKa = 4.95LGSAATAFSSVTSALVSSSWQGPASAAMASVAGGYY72 pKa = 10.01LGWLSSAGAQAAQAGGQARR91 pKa = 11.84IAAAAFEE98 pKa = 4.41ATRR101 pKa = 11.84AATVHH106 pKa = 6.49PAAVWANRR114 pKa = 11.84SQLVSLVASDD124 pKa = 4.62LLGFNAPAIAAVEE137 pKa = 3.85AAYY140 pKa = 10.15EE141 pKa = 4.06QMWAQDD147 pKa = 3.41VAAMFGYY154 pKa = 10.28HH155 pKa = 6.69AGASAAASALTPFIQLVQNPVASGEE180 pKa = 4.19ALVAAAQAAILSPPGRR196 pKa = 11.84VSIFNAGLADD206 pKa = 3.58VGVGNVGFGSVGDD219 pKa = 4.08GNVGAGNVGGSNVGFGNLGSLNFGSGNLGSFNVGSGNIGLYY260 pKa = 10.71NFGPGNLGSYY270 pKa = 10.35NVGLGNAGDD279 pKa = 3.91YY280 pKa = 11.39NFGFGNTGIGNIGFGNTGSHH300 pKa = 6.86NIGIGLTGDD309 pKa = 3.63GQVGFGGWNSGSANVGLFNSGIGNVGLFNSGTGNWGVGNSGEE351 pKa = 4.44LNTGLFNPGRR361 pKa = 11.84LNTGVFNTGLLNTGVGNAGSYY382 pKa = 10.96NSGSFNVGASNTGSWNAGDD401 pKa = 4.44TNTGWFNPGDD411 pKa = 3.9VNTGIGNTGDD421 pKa = 3.52VNTGGFNQGNLNNGFFWRR439 pKa = 11.84GDD441 pKa = 3.83GQGHH445 pKa = 7.23AGFDD449 pKa = 3.62YY450 pKa = 10.74TLTIPAIGLNLGADD464 pKa = 3.89VPLGIPVTGTIGTIVNGGPPEE485 pKa = 3.79ITVPGFTIPTLHH497 pKa = 6.29LTGDD501 pKa = 3.87ALFGTIGPIVVDD513 pKa = 5.16PITITGPSLNLTVGGGQSLQLGFSGPGVGPVVIPVLQVAAGPGVGNSTGVPSSGFFNSGVGGASGFGNVGGGSGWWNFGRR593 pKa = 11.84SSGVGNVGVLGSGVLNLGDD612 pKa = 4.49GVSGWFNTIPSMGSGVGNVGEE633 pKa = 4.03QLAGLFSAGPGGPSVFNLGLGNQGGLNLGHH663 pKa = 7.08ANVGDD668 pKa = 3.95FNLGGGNVGDD678 pKa = 4.6HH679 pKa = 6.15NVGGANVGDD688 pKa = 4.09ANVGVGNIGDD698 pKa = 4.11HH699 pKa = 5.98NVGGGNVGNVNVGGGNAGDD718 pKa = 4.36GNRR721 pKa = 11.84GWGNTGSFNVGFGNTGVGNFGLANQGSNNIGIGLSGDD758 pKa = 3.47NQIGFGGFNSGAGNVGLFNSGSNNIGFFNSGTGNFGVGNSGSFNTGIASTGSTNTGVFNTGLGNTGWANAGDD830 pKa = 4.11FNSGGFNAGAFNTGSFNPGDD850 pKa = 3.95TNTGWFNIGDD860 pKa = 4.44LNTGAFNTGDD870 pKa = 3.43INTGAFISGDD880 pKa = 3.49ANNGFFWRR888 pKa = 11.84GTGQGLIAADD898 pKa = 3.65YY899 pKa = 9.11TLTIPHH905 pKa = 6.92IPLTLGGGGFFEE917 pKa = 5.26LPITGSITGMTVQSFMVHH935 pKa = 7.04GIGDD939 pKa = 4.46SNTGIPLNLQLNVLGQQTDD958 pKa = 3.77EE959 pKa = 4.88IGVQVHH965 pKa = 6.14IPVIDD970 pKa = 3.8KK971 pKa = 10.8DD972 pKa = 3.87VTIPLPALPISLGITVDD989 pKa = 3.63NQIGAILVPPITINPITFNNVMVGNNTTSLSADD1022 pKa = 3.32VAGGIGPVTIPVFQLAAAPGFGNITGVPSSGFFNSGVGGGSGFGNSGSGVSGWWNVASLSSGYY1085 pKa = 10.38QNLGNLVSGFINKK1098 pKa = 9.69GEE1100 pKa = 4.1LLSGINNSNMLGLSTSGVGNVGDD1123 pKa = 3.93NVSGLFFQGADD1134 pKa = 2.57RR1135 pKa = 11.84VSIFNAGLADD1145 pKa = 3.58VGVGNVGFGSVGDD1158 pKa = 4.08GNVGAGNVGGSNVGFGNLGSLNFGSGNLGSFNVGSGNIGLYY1199 pKa = 10.71NFGPGNLGSYY1209 pKa = 10.35NVGLGNAGDD1218 pKa = 3.91YY1219 pKa = 11.39NFGFGNTGIGNIGFGNTGSHH1239 pKa = 6.86NIGIGLTGDD1248 pKa = 3.63GQVGFGGWNSGSANVGLFNSGAGNVGLFNSGTGNWGVGNSGEE1290 pKa = 4.44LNTGLFNPGRR1300 pKa = 11.84LNTGVFNTGLLNTGVGNAGSYY1321 pKa = 10.96NSGSFNVGASNTGSWNAGDD1340 pKa = 4.44TNTGWFNPGDD1350 pKa = 3.9VNTGIGNTGDD1360 pKa = 3.52VNTGGFNQGNLNNGFFWRR1378 pKa = 11.84GDD1380 pKa = 3.83GQGHH1384 pKa = 7.23AGFDD1388 pKa = 3.62YY1389 pKa = 10.74TLTIPAIALNLGVNVPLNIPITGHH1413 pKa = 6.75LGDD1416 pKa = 4.03IVIGSFTIPTLHH1428 pKa = 6.93LNGSNLTGTIGPIVVDD1444 pKa = 5.16PITITGPSLDD1454 pKa = 3.99LHH1456 pKa = 6.88VGGPGEE1462 pKa = 4.25SLQLDD1467 pKa = 3.72ISGPALGPVVIPVLQVAAGPGVGNSTGVPSSGFFNSGVGGASGFGNVGGGSGWWNFGGYY1526 pKa = 9.71SGVGNVGVLGSGVLNLGDD1544 pKa = 4.49GVSGWFNTVPSMGSGVGNVGEE1565 pKa = 4.03QLAGLFSAGPGGPSVFNLGLGNQGGLNLGHH1595 pKa = 7.08ANVGDD1600 pKa = 3.95FNLGGGNVGDD1610 pKa = 4.6HH1611 pKa = 6.15NVGGANVGDD1620 pKa = 4.09ANVGVGNIGDD1630 pKa = 4.11HH1631 pKa = 5.98NVGGGNVGNVNVGGSNAGDD1650 pKa = 3.88GNRR1653 pKa = 11.84GWGNTGSFNVGFGNTGVGNFGLANQGSNNIGIGLSGDD1690 pKa = 3.47NQIGFGGFNSGAGNVGLFNSGSNNIGFFNSGTGNFGVGNSGSFNTGIASTGSTNTGVFNTGLGNTGWANAGDD1762 pKa = 4.11FNSGGFNAGAFNTGSFNPGDD1782 pKa = 3.95TNTGWFNIGDD1792 pKa = 4.44LNTGAFNTGDD1802 pKa = 3.43INTGAFISGDD1812 pKa = 3.49ANNGFFWRR1820 pKa = 11.84GTGQGLIAADD1830 pKa = 3.65YY1831 pKa = 9.11TLTIPHH1837 pKa = 6.88IPLTLGLGGKK1847 pKa = 9.76LDD1849 pKa = 3.75IPITGQIAGLTVNPITLHH1867 pKa = 6.33GIGGSTTGIPVNAHH1881 pKa = 4.82FTLLGSTGGTTIHH1894 pKa = 6.38FNIPGTDD1901 pKa = 3.12LGFSFDD1907 pKa = 4.7LPSLPISIGVDD1918 pKa = 2.43IGDD1921 pKa = 3.76QIGAITIPQITINPITFNNVVVGGDD1946 pKa = 3.72TTSLSADD1953 pKa = 3.35VAGGIGPVTIPVFQLAAAPGFGNITGVPSSGFFNSGVGGGSGFGNSGSGVSGWWNVASLSSGYY2016 pKa = 10.38QNLGNLVSGFINKK2029 pKa = 9.69GEE2031 pKa = 4.1LLSGINNSNMLGLSTSGVGNVGDD2054 pKa = 3.93NVSGLFFQGADD2065 pKa = 2.57RR2066 pKa = 11.84VSIFNAGLADD2076 pKa = 3.58VGVGNVGFGSVGDD2089 pKa = 4.08GNVGAGNVGGSNVGFGNLGSLNFGSGNLGSFNVGSGNIGLYY2130 pKa = 10.71NFGPGNLGSYY2140 pKa = 10.35NVGLGNAGDD2149 pKa = 3.91YY2150 pKa = 11.39NFGFGNTGIGNIGFGNTGSHH2170 pKa = 6.86NIGIGLTGDD2179 pKa = 3.63GQVGFGGWNSGSANVGLFNSGAGNVGLFNSGTGNWGVGNSGEE2221 pKa = 4.44LNTGLFNPGRR2231 pKa = 11.84LNTGVFNTGLLNTGVGNAGSYY2252 pKa = 10.96NSGSFNVGASNTGSWNAGDD2271 pKa = 4.44TNTGWFNPGDD2281 pKa = 3.9VNTGIGNTGDD2291 pKa = 3.52VNTGGFNQGNLNNGFFWRR2309 pKa = 11.84GDD2311 pKa = 3.83GQGHH2315 pKa = 7.23AGFDD2319 pKa = 3.62YY2320 pKa = 10.74TLTIPAIALNLGVNVPLNIPITGHH2344 pKa = 6.63LGDD2347 pKa = 4.07IVVDD2351 pKa = 3.99PFTIPVLHH2359 pKa = 6.22LTGTGGNSLTGTIGPIVSDD2378 pKa = 3.89QITITGPSLNLTLGGPGEE2396 pKa = 4.13SLQLGFSGPALGPVVIPVLQVAAGPGVGNSTGVPSSGFFNSGVGGASGFGNVGGGSGWWNFGGYY2460 pKa = 9.71SGVGNVGVLGSGVLNLGDD2478 pKa = 4.49GVSGWFNTVPSMGSGVGNVGEE2499 pKa = 4.03QLAGLFSAGPGGPSVFNLGLGNQGGLNLGHH2529 pKa = 7.08ANVGDD2534 pKa = 3.95FNLGGGNVGDD2544 pKa = 4.6HH2545 pKa = 6.15NVGGANVGDD2554 pKa = 4.09ANVGVGNIGDD2564 pKa = 4.11HH2565 pKa = 5.98NVGGGNVGNVNVGGGNAGDD2584 pKa = 4.36GNRR2587 pKa = 11.84GWGNTGSFNVGFGNTGVGNFGLANQGSNNIGIGLSGDD2624 pKa = 3.47NQIGFGGFNSGAGNVGLFNSGSNNIGFFNSGTGNFGVGNSGSFNTGIASTGSTNTGVFNTGLGNTGWANAGDD2696 pKa = 4.11FNSGGFNAGAFNTGSFNPGDD2716 pKa = 3.95TNTGWFNIGDD2726 pKa = 4.44LNTGAFNTGDD2736 pKa = 3.43INTGAFISGDD2746 pKa = 3.49ANNGFFWRR2754 pKa = 11.84GTGQGLIAADD2764 pKa = 3.65YY2765 pKa = 9.11TLTIPHH2771 pKa = 6.88IPLTLGLGGKK2781 pKa = 9.76LDD2783 pKa = 3.75IPITGQIAGLTVNQFTLHH2801 pKa = 6.46GEE2803 pKa = 4.08NGPGIPLNLLLAIDD2817 pKa = 4.57ADD2819 pKa = 4.06SGGTSIHH2826 pKa = 6.38VGIPDD2831 pKa = 3.55TDD2833 pKa = 3.17IGFDD2837 pKa = 3.47IPIDD2841 pKa = 3.91GLPITLTLPLNSEE2854 pKa = 4.53LSPIVIEE2861 pKa = 5.08PITIGAIPLDD2871 pKa = 3.94LTVGGDD2877 pKa = 3.45TTQLGTALAAGIGPITATLFHH2898 pKa = 6.78VSPVPGFGNSTGSPSSGFFNSGAGGSSGFGNIGDD2932 pKa = 4.1TLSGFWNVGSEE2943 pKa = 4.18GSGFEE2948 pKa = 4.09NYY2950 pKa = 10.03GGSLLSGITNLGGSLSGIDD2969 pKa = 3.51NTSSLGLALAGVVSGLGNIGSQLSGLFLSGSVPP3002 pKa = 3.3

Molecular weight: 292.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A498QQZ4|A0A498QQZ4_9MYCO Tnp_DDE_dom domain-containing protein OS=Mycobacterium pseudokansasii OX=2341080 GN=LAUMK142_01753 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5730

0

5730

1855580

29

4336

323.8

34.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.303 ± 0.052

0.877 ± 0.011

5.863 ± 0.027

4.772 ± 0.032

2.993 ± 0.022

9.493 ± 0.091

2.285 ± 0.017

4.174 ± 0.021

2.027 ± 0.022

9.749 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.027 ± 0.013

2.467 ± 0.049

5.975 ± 0.036

3.132 ± 0.017

7.263 ± 0.048

5.516 ± 0.027

5.974 ± 0.025

8.436 ± 0.036

1.519 ± 0.015

2.156 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski