Panicum ecklonii-associated virus

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses

Average proteome isoelectric point is 8.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345BKA6|A0A345BKA6_9VIRU Putative capsid protein OS=Panicum ecklonii-associated virus OX=2282645 PE=4 SV=1
MM1 pKa = 7.44TKK3 pKa = 9.93QGSLAKK9 pKa = 9.62HH10 pKa = 4.79WVFTSFADD18 pKa = 3.47EE19 pKa = 4.24VVFFSNVDD27 pKa = 3.2VQYY30 pKa = 10.99CIFGNEE36 pKa = 4.01QCPKK40 pKa = 7.63TGRR43 pKa = 11.84WHH45 pKa = 5.3KK46 pKa = 10.32QGYY49 pKa = 8.13VVFKK53 pKa = 9.97VKK55 pKa = 10.58KK56 pKa = 10.46RR57 pKa = 11.84MTALKK62 pKa = 10.29KK63 pKa = 9.87IDD65 pKa = 3.91PTAHH69 pKa = 5.99WEE71 pKa = 3.99IKK73 pKa = 10.17RR74 pKa = 11.84GSVQQAIDD82 pKa = 3.68YY83 pKa = 8.81CKK85 pKa = 10.56KK86 pKa = 10.56DD87 pKa = 3.83DD88 pKa = 3.66DD89 pKa = 3.99WYY91 pKa = 10.48EE92 pKa = 3.95YY93 pKa = 11.26GEE95 pKa = 4.37PPVEE99 pKa = 4.06EE100 pKa = 3.88RR101 pKa = 11.84SGANAYY107 pKa = 10.34AEE109 pKa = 4.68AIAFAEE115 pKa = 4.36AGDD118 pKa = 4.17LEE120 pKa = 4.9SVKK123 pKa = 10.26QFHH126 pKa = 7.28PGTFLRR132 pKa = 11.84YY133 pKa = 9.35KK134 pKa = 9.64RR135 pKa = 11.84TLEE138 pKa = 3.8GLIKK142 pKa = 10.0YY143 pKa = 10.41DD144 pKa = 4.39CVEE147 pKa = 4.06LDD149 pKa = 3.83APCGYY154 pKa = 9.8WIYY157 pKa = 10.84GKK159 pKa = 9.66PGSGKK164 pKa = 10.07DD165 pKa = 3.59GSVMEE170 pKa = 5.01LKK172 pKa = 10.66PFVKK176 pKa = 10.01SHH178 pKa = 5.41SKK180 pKa = 8.64WWDD183 pKa = 3.42GYY185 pKa = 10.31KK186 pKa = 10.25NEE188 pKa = 4.9PYY190 pKa = 10.7VVLQDD195 pKa = 4.85LDD197 pKa = 4.89HH198 pKa = 7.5IDD200 pKa = 3.86AKK202 pKa = 10.75WIGSYY207 pKa = 10.36LKK209 pKa = 9.96QWSDD213 pKa = 3.01RR214 pKa = 11.84YY215 pKa = 11.01SFNAEE220 pKa = 3.94YY221 pKa = 10.37KK222 pKa = 9.6GGSMKK227 pKa = 9.92IRR229 pKa = 11.84PKK231 pKa = 10.44RR232 pKa = 11.84FYY234 pKa = 9.4VTSNYY239 pKa = 10.19KK240 pKa = 10.01IGEE243 pKa = 4.23LFSDD247 pKa = 5.14EE248 pKa = 3.74ICLALKK254 pKa = 10.48RR255 pKa = 11.84RR256 pKa = 11.84FHH258 pKa = 7.27VILFDD263 pKa = 3.94EE264 pKa = 5.04DD265 pKa = 3.98VVLPRR270 pKa = 11.84PVVNFVSKK278 pKa = 10.8KK279 pKa = 10.17SLIDD283 pKa = 3.36FF284 pKa = 4.76

Molecular weight: 32.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345BKA6|A0A345BKA6_9VIRU Putative capsid protein OS=Panicum ecklonii-associated virus OX=2282645 PE=4 SV=1
MM1 pKa = 7.24PAVRR5 pKa = 11.84RR6 pKa = 11.84LARR9 pKa = 11.84SLVRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AVRR18 pKa = 11.84RR19 pKa = 11.84PAYY22 pKa = 9.77RR23 pKa = 11.84RR24 pKa = 11.84KK25 pKa = 9.58KK26 pKa = 7.89YY27 pKa = 8.41VRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84FHH34 pKa = 6.99RR35 pKa = 11.84KK36 pKa = 9.24RR37 pKa = 11.84FLHH40 pKa = 4.98TQNVVKK46 pKa = 7.8YY47 pKa = 9.65HH48 pKa = 6.44CKK50 pKa = 10.39QYY52 pKa = 10.01DD53 pKa = 3.38TGIMYY58 pKa = 8.85PRR60 pKa = 11.84TGSGTTFVTDD70 pKa = 3.08VQTFQLPLYY79 pKa = 10.79ANAFFNGNQQFYY91 pKa = 10.87KK92 pKa = 10.93NLDD95 pKa = 3.39EE96 pKa = 4.05YY97 pKa = 11.54HH98 pKa = 6.86FIKK101 pKa = 10.65FNYY104 pKa = 8.44IAVHH108 pKa = 5.01VKK110 pKa = 8.19EE111 pKa = 4.9LNWIGYY117 pKa = 6.17TKK119 pKa = 9.82TDD121 pKa = 2.54RR122 pKa = 11.84VVPPEE127 pKa = 4.55GGDD130 pKa = 3.46PYY132 pKa = 10.54PAVSGVTAMQMQNHH146 pKa = 6.8PVYY149 pKa = 10.77IMWDD153 pKa = 3.27IEE155 pKa = 3.85EE156 pKa = 5.36DD157 pKa = 3.91FAFDD161 pKa = 3.92TNDD164 pKa = 3.05KK165 pKa = 10.68VQITSQQLAQYY176 pKa = 9.93QGAKK180 pKa = 7.68TLRR183 pKa = 11.84TTSKK187 pKa = 10.72KK188 pKa = 8.78PVKK191 pKa = 10.03FIWRR195 pKa = 11.84FPVPWRR201 pKa = 11.84QFYY204 pKa = 10.79SCYY207 pKa = 10.12NFKK210 pKa = 10.8QITHH214 pKa = 5.25SNRR217 pKa = 11.84WGTVMEE223 pKa = 4.32EE224 pKa = 3.73LSGIKK229 pKa = 9.97NLRR232 pKa = 11.84APKK235 pKa = 9.92NLLMCHH241 pKa = 5.95PNWWGSSLPVNAALDD256 pKa = 3.75AADD259 pKa = 5.58FYY261 pKa = 11.45GQYY264 pKa = 10.83AIVYY268 pKa = 8.34HH269 pKa = 6.41LGVTFRR275 pKa = 11.84GRR277 pKa = 11.84SLMGSATSYY286 pKa = 11.1APPAQVTSADD296 pKa = 3.68EE297 pKa = 3.91

Molecular weight: 34.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

581

284

297

290.5

33.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.54 ± 0.658

1.549 ± 0.409

5.508 ± 1.113

4.475 ± 1.352

5.852 ± 0.097

6.196 ± 0.614

2.754 ± 0.21

4.303 ± 0.455

8.09 ± 1.795

5.852 ± 0.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.065 ± 0.477

3.787 ± 0.959

4.991 ± 0.556

4.475 ± 0.948

6.368 ± 1.555

5.508 ± 0.602

4.819 ± 1.453

7.917 ± 0.131

2.754 ± 0.301

6.196 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski