Amino acid dipepetide frequency for Microbacterium phage Sharkboy

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.581AlaAla: 13.581 ± 1.372
0.152AlaCys: 0.152 ± 0.103
7.056AlaAsp: 7.056 ± 0.786
7.208AlaGlu: 7.208 ± 0.819
3.794AlaPhe: 3.794 ± 0.527
12.443AlaGly: 12.443 ± 0.935
1.669AlaHis: 1.669 ± 0.366
4.932AlaIle: 4.932 ± 0.647
5.69AlaLys: 5.69 ± 0.813
11.077AlaLeu: 11.077 ± 1.002
2.2AlaMet: 2.2 ± 0.419
3.414AlaAsn: 3.414 ± 0.61
6.373AlaPro: 6.373 ± 0.622
4.932AlaGln: 4.932 ± 0.596
7.587AlaArg: 7.587 ± 0.79
5.387AlaSer: 5.387 ± 0.691
5.918AlaThr: 5.918 ± 0.81
8.498AlaVal: 8.498 ± 0.838
2.276AlaTrp: 2.276 ± 0.44
2.656AlaTyr: 2.656 ± 0.544
0.0AlaXaa: 0.0 ± 0.0
Cys
0.228CysAla: 0.228 ± 0.148
0.076CysCys: 0.076 ± 0.079
0.228CysAsp: 0.228 ± 0.129
0.076CysGlu: 0.076 ± 0.083
0.303CysPhe: 0.303 ± 0.156
0.683CysGly: 0.683 ± 0.268
0.0CysHis: 0.0 ± 0.0
0.076CysIle: 0.076 ± 0.071
0.379CysLys: 0.379 ± 0.173
0.076CysLeu: 0.076 ± 0.071
0.076CysMet: 0.076 ± 0.074
0.228CysAsn: 0.228 ± 0.131
0.607CysPro: 0.607 ± 0.216
0.076CysGln: 0.076 ± 0.074
0.303CysArg: 0.303 ± 0.172
0.303CysSer: 0.303 ± 0.145
0.152CysThr: 0.152 ± 0.108
0.303CysVal: 0.303 ± 0.162
0.076CysTrp: 0.076 ± 0.075
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
6.373AspAla: 6.373 ± 0.806
0.379AspCys: 0.379 ± 0.17
3.338AspAsp: 3.338 ± 0.688
3.49AspGlu: 3.49 ± 0.646
2.2AspPhe: 2.2 ± 0.378
6.98AspGly: 6.98 ± 0.833
1.442AspHis: 1.442 ± 0.33
1.745AspIle: 1.745 ± 0.348
1.745AspLys: 1.745 ± 0.499
6.601AspLeu: 6.601 ± 0.97
1.062AspMet: 1.062 ± 0.286
0.986AspAsn: 0.986 ± 0.284
4.325AspPro: 4.325 ± 0.551
2.124AspGln: 2.124 ± 0.424
3.187AspArg: 3.187 ± 0.587
3.566AspSer: 3.566 ± 0.518
3.338AspThr: 3.338 ± 0.596
5.235AspVal: 5.235 ± 0.637
1.593AspTrp: 1.593 ± 0.343
2.58AspTyr: 2.58 ± 0.345
0.0AspXaa: 0.0 ± 0.0
Glu
8.649GluAla: 8.649 ± 0.813
0.076GluCys: 0.076 ± 0.071
4.325GluAsp: 4.325 ± 0.563
5.008GluGlu: 5.008 ± 0.866
2.58GluPhe: 2.58 ± 0.356
4.401GluGly: 4.401 ± 0.505
1.366GluHis: 1.366 ± 0.283
1.062GluIle: 1.062 ± 0.295
1.973GluLys: 1.973 ± 0.49
7.436GluLeu: 7.436 ± 0.773
1.29GluMet: 1.29 ± 0.295
1.517GluAsn: 1.517 ± 0.418
3.111GluPro: 3.111 ± 0.507
2.124GluGln: 2.124 ± 0.317
2.959GluArg: 2.959 ± 0.523
2.2GluSer: 2.2 ± 0.588
3.642GluThr: 3.642 ± 0.405
5.387GluVal: 5.387 ± 0.705
1.366GluTrp: 1.366 ± 0.344
1.442GluTyr: 1.442 ± 0.327
0.0GluXaa: 0.0 ± 0.0
Phe
2.807PheAla: 2.807 ± 0.581
0.303PheCys: 0.303 ± 0.157
1.897PheAsp: 1.897 ± 0.546
2.352PheGlu: 2.352 ± 0.443
0.531PhePhe: 0.531 ± 0.252
2.58PheGly: 2.58 ± 0.442
0.228PheHis: 0.228 ± 0.118
1.366PheIle: 1.366 ± 0.35
1.669PheLys: 1.669 ± 0.29
2.352PheLeu: 2.352 ± 0.445
0.835PheMet: 0.835 ± 0.229
1.593PheAsn: 1.593 ± 0.291
1.214PhePro: 1.214 ± 0.267
1.517PheGln: 1.517 ± 0.313
3.338PheArg: 3.338 ± 0.497
1.821PheSer: 1.821 ± 0.314
2.58PheThr: 2.58 ± 0.463
2.959PheVal: 2.959 ± 0.494
0.835PheTrp: 0.835 ± 0.332
0.759PheTyr: 0.759 ± 0.22
0.0PheXaa: 0.0 ± 0.0
Gly
9.029GlyAla: 9.029 ± 0.939
0.531GlyCys: 0.531 ± 0.242
6.146GlyAsp: 6.146 ± 0.7
4.249GlyGlu: 4.249 ± 0.608
2.883GlyPhe: 2.883 ± 0.573
6.98GlyGly: 6.98 ± 1.01
1.517GlyHis: 1.517 ± 0.403
3.49GlyIle: 3.49 ± 0.608
3.945GlyLys: 3.945 ± 0.648
7.36GlyLeu: 7.36 ± 0.709
2.504GlyMet: 2.504 ± 0.548
2.731GlyAsn: 2.731 ± 0.74
3.794GlyPro: 3.794 ± 0.769
4.173GlyGln: 4.173 ± 0.798
5.083GlyArg: 5.083 ± 0.461
4.78GlySer: 4.78 ± 0.716
6.222GlyThr: 6.222 ± 0.988
7.815GlyVal: 7.815 ± 0.868
2.276GlyTrp: 2.276 ± 0.422
2.883GlyTyr: 2.883 ± 0.475
0.0GlyXaa: 0.0 ± 0.0
His
2.352HisAla: 2.352 ± 0.496
0.076HisCys: 0.076 ± 0.075
0.759HisAsp: 0.759 ± 0.237
1.062HisGlu: 1.062 ± 0.304
1.214HisPhe: 1.214 ± 0.272
1.366HisGly: 1.366 ± 0.32
0.455HisHis: 0.455 ± 0.164
0.303HisIle: 0.303 ± 0.151
0.759HisLys: 0.759 ± 0.247
2.428HisLeu: 2.428 ± 0.42
0.303HisMet: 0.303 ± 0.141
0.303HisAsn: 0.303 ± 0.16
1.29HisPro: 1.29 ± 0.273
0.455HisGln: 0.455 ± 0.157
0.683HisArg: 0.683 ± 0.22
0.455HisSer: 0.455 ± 0.241
0.835HisThr: 0.835 ± 0.234
1.517HisVal: 1.517 ± 0.381
0.152HisTrp: 0.152 ± 0.102
0.91HisTyr: 0.91 ± 0.259
0.0HisXaa: 0.0 ± 0.0
Ile
4.401IleAla: 4.401 ± 0.532
0.076IleCys: 0.076 ± 0.072
2.883IleAsp: 2.883 ± 0.326
2.807IleGlu: 2.807 ± 0.409
0.683IlePhe: 0.683 ± 0.247
3.263IleGly: 3.263 ± 0.587
0.91IleHis: 0.91 ± 0.272
1.821IleIle: 1.821 ± 0.301
1.973IleLys: 1.973 ± 0.437
3.035IleLeu: 3.035 ± 0.487
0.379IleMet: 0.379 ± 0.176
1.138IleAsn: 1.138 ± 0.335
1.366IlePro: 1.366 ± 0.376
0.986IleGln: 0.986 ± 0.312
2.352IleArg: 2.352 ± 0.451
1.973IleSer: 1.973 ± 0.536
2.656IleThr: 2.656 ± 0.623
3.187IleVal: 3.187 ± 0.564
0.835IleTrp: 0.835 ± 0.256
0.91IleTyr: 0.91 ± 0.254
0.0IleXaa: 0.0 ± 0.0
Lys
5.918LysAla: 5.918 ± 1.077
0.683LysCys: 0.683 ± 0.242
1.897LysAsp: 1.897 ± 0.44
2.049LysGlu: 2.049 ± 0.351
1.214LysPhe: 1.214 ± 0.381
3.414LysGly: 3.414 ± 0.425
0.531LysHis: 0.531 ± 0.244
1.138LysIle: 1.138 ± 0.331
1.593LysLys: 1.593 ± 0.385
3.869LysLeu: 3.869 ± 0.726
0.986LysMet: 0.986 ± 0.271
0.835LysAsn: 0.835 ± 0.211
2.807LysPro: 2.807 ± 0.536
1.669LysGln: 1.669 ± 0.413
3.49LysArg: 3.49 ± 0.388
2.352LysSer: 2.352 ± 0.595
2.656LysThr: 2.656 ± 0.447
2.959LysVal: 2.959 ± 0.48
0.455LysTrp: 0.455 ± 0.173
0.531LysTyr: 0.531 ± 0.197
0.0LysXaa: 0.0 ± 0.0
Leu
10.091LeuAla: 10.091 ± 1.096
0.379LeuCys: 0.379 ± 0.169
5.463LeuAsp: 5.463 ± 0.719
5.008LeuGlu: 5.008 ± 0.739
2.883LeuPhe: 2.883 ± 0.409
7.739LeuGly: 7.739 ± 0.737
1.745LeuHis: 1.745 ± 0.366
3.414LeuIle: 3.414 ± 0.557
3.566LeuLys: 3.566 ± 0.636
6.753LeuLeu: 6.753 ± 0.684
2.656LeuMet: 2.656 ± 0.418
2.959LeuAsn: 2.959 ± 0.53
4.476LeuPro: 4.476 ± 0.574
3.794LeuGln: 3.794 ± 0.85
5.69LeuArg: 5.69 ± 0.662
4.932LeuSer: 4.932 ± 0.451
5.159LeuThr: 5.159 ± 0.797
7.891LeuVal: 7.891 ± 0.718
1.442LeuTrp: 1.442 ± 0.333
1.821LeuTyr: 1.821 ± 0.294
0.0LeuXaa: 0.0 ± 0.0
Met
3.414MetAla: 3.414 ± 0.543
0.0MetCys: 0.0 ± 0.0
1.669MetAsp: 1.669 ± 0.353
1.29MetGlu: 1.29 ± 0.299
0.531MetPhe: 0.531 ± 0.193
1.062MetGly: 1.062 ± 0.268
0.076MetHis: 0.076 ± 0.087
0.607MetIle: 0.607 ± 0.189
1.214MetLys: 1.214 ± 0.336
1.669MetLeu: 1.669 ± 0.321
0.228MetMet: 0.228 ± 0.133
0.607MetAsn: 0.607 ± 0.243
0.759MetPro: 0.759 ± 0.199
0.607MetGln: 0.607 ± 0.199
0.91MetArg: 0.91 ± 0.258
1.669MetSer: 1.669 ± 0.338
2.049MetThr: 2.049 ± 0.377
1.138MetVal: 1.138 ± 0.273
0.455MetTrp: 0.455 ± 0.232
0.303MetTyr: 0.303 ± 0.146
0.0MetXaa: 0.0 ± 0.0
Asn
2.656AsnAla: 2.656 ± 0.424
0.228AsnCys: 0.228 ± 0.122
1.517AsnAsp: 1.517 ± 0.313
1.062AsnGlu: 1.062 ± 0.32
0.759AsnPhe: 0.759 ± 0.247
3.642AsnGly: 3.642 ± 0.776
0.683AsnHis: 0.683 ± 0.266
0.683AsnIle: 0.683 ± 0.269
0.986AsnLys: 0.986 ± 0.347
2.352AsnLeu: 2.352 ± 0.418
0.531AsnMet: 0.531 ± 0.256
0.835AsnAsn: 0.835 ± 0.246
1.821AsnPro: 1.821 ± 0.28
1.442AsnGln: 1.442 ± 0.287
2.352AsnArg: 2.352 ± 0.532
1.821AsnSer: 1.821 ± 0.399
1.593AsnThr: 1.593 ± 0.347
2.2AsnVal: 2.2 ± 0.423
0.835AsnTrp: 0.835 ± 0.275
0.91AsnTyr: 0.91 ± 0.25
0.0AsnXaa: 0.0 ± 0.0
Pro
8.27ProAla: 8.27 ± 0.713
0.303ProCys: 0.303 ± 0.162
3.49ProAsp: 3.49 ± 0.551
4.401ProGlu: 4.401 ± 0.615
2.049ProPhe: 2.049 ± 0.359
5.083ProGly: 5.083 ± 0.614
0.986ProHis: 0.986 ± 0.324
2.124ProIle: 2.124 ± 0.477
2.124ProLys: 2.124 ± 0.517
4.021ProLeu: 4.021 ± 0.556
0.91ProMet: 0.91 ± 0.237
1.745ProAsn: 1.745 ± 0.397
1.517ProPro: 1.517 ± 0.382
1.669ProGln: 1.669 ± 0.559
2.124ProArg: 2.124 ± 0.44
3.566ProSer: 3.566 ± 0.593
2.656ProThr: 2.656 ± 0.424
3.869ProVal: 3.869 ± 0.534
1.214ProTrp: 1.214 ± 0.314
0.379ProTyr: 0.379 ± 0.171
0.0ProXaa: 0.0 ± 0.0
Gln
4.325GlnAla: 4.325 ± 0.525
0.152GlnCys: 0.152 ± 0.159
2.276GlnAsp: 2.276 ± 0.457
2.2GlnGlu: 2.2 ± 0.498
1.442GlnPhe: 1.442 ± 0.343
2.504GlnGly: 2.504 ± 0.375
0.531GlnHis: 0.531 ± 0.202
0.835GlnIle: 0.835 ± 0.25
1.29GlnLys: 1.29 ± 0.267
3.566GlnLeu: 3.566 ± 0.543
0.531GlnMet: 0.531 ± 0.188
1.214GlnAsn: 1.214 ± 0.27
1.745GlnPro: 1.745 ± 0.455
1.138GlnGln: 1.138 ± 0.271
2.2GlnArg: 2.2 ± 0.368
1.669GlnSer: 1.669 ± 0.312
2.428GlnThr: 2.428 ± 0.494
4.021GlnVal: 4.021 ± 0.505
0.683GlnTrp: 0.683 ± 0.263
0.91GlnTyr: 0.91 ± 0.241
0.0GlnXaa: 0.0 ± 0.0
Arg
6.904ArgAla: 6.904 ± 0.847
0.228ArgCys: 0.228 ± 0.183
4.249ArgAsp: 4.249 ± 0.587
4.021ArgGlu: 4.021 ± 0.706
2.276ArgPhe: 2.276 ± 0.371
5.159ArgGly: 5.159 ± 0.563
0.91ArgHis: 0.91 ± 0.276
2.428ArgIle: 2.428 ± 0.459
2.124ArgLys: 2.124 ± 0.369
6.373ArgLeu: 6.373 ± 0.769
0.986ArgMet: 0.986 ± 0.314
1.897ArgAsn: 1.897 ± 0.277
2.959ArgPro: 2.959 ± 0.485
2.049ArgGln: 2.049 ± 0.33
5.69ArgArg: 5.69 ± 0.802
3.642ArgSer: 3.642 ± 0.513
4.173ArgThr: 4.173 ± 0.45
5.311ArgVal: 5.311 ± 0.841
1.214ArgTrp: 1.214 ± 0.329
1.366ArgTyr: 1.366 ± 0.331
0.0ArgXaa: 0.0 ± 0.0
Ser
6.222SerAla: 6.222 ± 0.685
0.152SerCys: 0.152 ± 0.102
3.338SerAsp: 3.338 ± 0.484
2.959SerGlu: 2.959 ± 0.462
2.124SerPhe: 2.124 ± 0.397
6.222SerGly: 6.222 ± 0.798
0.683SerHis: 0.683 ± 0.208
2.504SerIle: 2.504 ± 0.513
2.276SerLys: 2.276 ± 0.448
4.552SerLeu: 4.552 ± 0.667
1.442SerMet: 1.442 ± 0.4
1.821SerAsn: 1.821 ± 0.434
3.111SerPro: 3.111 ± 0.474
1.669SerGln: 1.669 ± 0.372
3.263SerArg: 3.263 ± 0.432
2.959SerSer: 2.959 ± 0.503
3.566SerThr: 3.566 ± 0.559
4.704SerVal: 4.704 ± 0.713
1.138SerTrp: 1.138 ± 0.262
1.062SerTyr: 1.062 ± 0.243
0.0SerXaa: 0.0 ± 0.0
Thr
7.284ThrAla: 7.284 ± 0.884
0.076ThrCys: 0.076 ± 0.088
3.718ThrAsp: 3.718 ± 0.549
3.566ThrGlu: 3.566 ± 0.506
2.352ThrPhe: 2.352 ± 0.468
5.235ThrGly: 5.235 ± 0.656
0.986ThrHis: 0.986 ± 0.282
3.718ThrIle: 3.718 ± 0.57
2.883ThrLys: 2.883 ± 0.426
4.628ThrLeu: 4.628 ± 0.66
0.607ThrMet: 0.607 ± 0.219
1.214ThrAsn: 1.214 ± 0.331
4.552ThrPro: 4.552 ± 0.539
0.91ThrGln: 0.91 ± 0.235
3.49ThrArg: 3.49 ± 0.591
4.173ThrSer: 4.173 ± 0.552
3.718ThrThr: 3.718 ± 0.621
5.766ThrVal: 5.766 ± 0.805
1.366ThrTrp: 1.366 ± 0.331
1.669ThrTyr: 1.669 ± 0.396
0.0ThrXaa: 0.0 ± 0.0
Val
9.939ValAla: 9.939 ± 0.731
0.152ValCys: 0.152 ± 0.118
4.932ValAsp: 4.932 ± 0.688
6.146ValGlu: 6.146 ± 0.631
2.807ValPhe: 2.807 ± 0.618
5.69ValGly: 5.69 ± 0.893
1.897ValHis: 1.897 ± 0.388
3.566ValIle: 3.566 ± 0.525
3.414ValLys: 3.414 ± 0.573
6.222ValLeu: 6.222 ± 0.721
1.214ValMet: 1.214 ± 0.272
2.276ValAsn: 2.276 ± 0.413
4.78ValPro: 4.78 ± 0.669
2.428ValGln: 2.428 ± 0.385
5.994ValArg: 5.994 ± 0.69
5.159ValSer: 5.159 ± 0.615
5.615ValThr: 5.615 ± 0.708
8.498ValVal: 8.498 ± 0.801
1.745ValTrp: 1.745 ± 0.417
2.352ValTyr: 2.352 ± 0.474
0.0ValXaa: 0.0 ± 0.0
Trp
2.049TrpAla: 2.049 ± 0.503
0.076TrpCys: 0.076 ± 0.076
0.835TrpAsp: 0.835 ± 0.317
1.214TrpGlu: 1.214 ± 0.384
0.379TrpPhe: 0.379 ± 0.16
1.745TrpGly: 1.745 ± 0.344
0.379TrpHis: 0.379 ± 0.175
0.91TrpIle: 0.91 ± 0.337
0.835TrpLys: 0.835 ± 0.201
2.124TrpLeu: 2.124 ± 0.388
0.683TrpMet: 0.683 ± 0.223
1.062TrpAsn: 1.062 ± 0.333
0.455TrpPro: 0.455 ± 0.182
1.138TrpGln: 1.138 ± 0.432
1.138TrpArg: 1.138 ± 0.395
1.593TrpSer: 1.593 ± 0.33
1.517TrpThr: 1.517 ± 0.349
1.821TrpVal: 1.821 ± 0.272
0.152TrpTrp: 0.152 ± 0.105
0.455TrpTyr: 0.455 ± 0.181
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.352TyrAla: 2.352 ± 0.423
0.152TyrCys: 0.152 ± 0.118
2.276TyrAsp: 2.276 ± 0.396
1.745TyrGlu: 1.745 ± 0.312
0.531TyrPhe: 0.531 ± 0.174
2.2TyrGly: 2.2 ± 0.381
0.683TyrHis: 0.683 ± 0.215
0.91TyrIle: 0.91 ± 0.255
0.607TyrLys: 0.607 ± 0.243
1.366TyrLeu: 1.366 ± 0.316
0.759TyrMet: 0.759 ± 0.203
0.531TyrAsn: 0.531 ± 0.201
1.442TyrPro: 1.442 ± 0.301
0.759TyrGln: 0.759 ± 0.278
2.124TyrArg: 2.124 ± 0.48
1.745TyrSer: 1.745 ± 0.5
1.442TyrThr: 1.442 ± 0.268
1.821TyrVal: 1.821 ± 0.35
0.455TyrTrp: 0.455 ± 0.179
0.303TyrTyr: 0.303 ± 0.145
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (13181 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski