Streptomyces sp. CB01881

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7146 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L0MYV5|A0A2L0MYV5_9ACTN Oxidoreductase OS=Streptomyces sp. CB01881 OX=2078691 GN=C2142_07515 PE=4 SV=1
MM1 pKa = 7.01AQKK4 pKa = 8.91ITVEE8 pKa = 4.33SPAAPVTGFADD19 pKa = 3.87EE20 pKa = 4.58FDD22 pKa = 4.25LDD24 pKa = 3.68VRR26 pKa = 11.84TVEE29 pKa = 4.23TADD32 pKa = 3.47AASLQVLTDD41 pKa = 4.17DD42 pKa = 5.16GCGSTCGACTTGVHH56 pKa = 6.14

Molecular weight: 5.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L0N4L2|A0A2L0N4L2_9ACTN Phosphoribosylaminoimidazole-succinocarboxamide synthase OS=Streptomyces sp. CB01881 OX=2078691 GN=purC PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.34GRR40 pKa = 11.84SAISAA45 pKa = 3.73

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7146

0

7146

2301491

22

6410

322.1

34.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.454 ± 0.051

0.787 ± 0.008

5.691 ± 0.023

5.41 ± 0.033

2.647 ± 0.013

9.833 ± 0.029

2.284 ± 0.014

2.887 ± 0.02

1.821 ± 0.02

10.702 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.543 ± 0.011

1.732 ± 0.018

6.532 ± 0.03

2.846 ± 0.019

8.097 ± 0.033

4.812 ± 0.024

6.064 ± 0.027

8.343 ± 0.027

1.527 ± 0.012

1.987 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski