Influenza C virus (strain C/Ann Arbor/1/1950)
Average proteome isoelectric point is 7.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q01639|NS1_INCAA Non-structural protein 1 OS=Influenza C virus (strain C/Ann Arbor/1/1950) OX=11553 GN=NS PE=2 SV=2
MM1 pKa = 7.28 FFSLLLMLGLTEE13 pKa = 4.17 AEE15 pKa = 4.5 KK16 pKa = 10.71 IKK18 pKa = 10.49 ICLQKK23 pKa = 10.46 QVNSSFSLHH32 pKa = 6.18 NGFGGNLYY40 pKa = 9.14 ATEE43 pKa = 4.17 EE44 pKa = 3.89 KK45 pKa = 10.88 RR46 pKa = 11.84 MFEE49 pKa = 4.06 LVKK52 pKa = 10.01 PKK54 pKa = 10.77 AGASVLNQSTWIGFGDD70 pKa = 3.97 SRR72 pKa = 11.84 TDD74 pKa = 3.11 KK75 pKa = 11.14 SNSAFPRR82 pKa = 11.84 SADD85 pKa = 3.48 VSAKK89 pKa = 9.58 TADD92 pKa = 3.48 KK93 pKa = 10.49 FRR95 pKa = 11.84 SLSGGSLMLSMFGPPGKK112 pKa = 9.91 VDD114 pKa = 3.66 YY115 pKa = 10.63 LYY117 pKa = 10.65 QGCGKK122 pKa = 9.87 HH123 pKa = 5.19 KK124 pKa = 10.57 VFYY127 pKa = 10.67 EE128 pKa = 4.31 GVNWSPHH135 pKa = 4.95 AAINCYY141 pKa = 9.54 RR142 pKa = 11.84 KK143 pKa = 9.49 NWTDD147 pKa = 2.75 IKK149 pKa = 11.35 LNFQKK154 pKa = 10.74 NIYY157 pKa = 8.76 EE158 pKa = 4.29 LASQSHH164 pKa = 6.17 CMSLVNALDD173 pKa = 3.42 KK174 pKa = 10.47 TIPLQATAGVAKK186 pKa = 10.38 NCNNSFLKK194 pKa = 10.92 NPALYY199 pKa = 8.36 TQEE202 pKa = 4.12 VNPSVEE208 pKa = 4.03 KK209 pKa = 10.76 CGKK212 pKa = 9.53 EE213 pKa = 3.49 NLAFFTLPTQFGTYY227 pKa = 7.66 EE228 pKa = 4.29 CKK230 pKa = 10.21 LHH232 pKa = 6.73 LVASCYY238 pKa = 10.19 FIYY241 pKa = 10.31 DD242 pKa = 3.38 SKK244 pKa = 11.26 EE245 pKa = 3.89 VYY247 pKa = 9.91 NKK249 pKa = 10.22 RR250 pKa = 11.84 GCDD253 pKa = 2.93 NYY255 pKa = 10.72 FQVIYY260 pKa = 10.21 DD261 pKa = 3.56 SSGKK265 pKa = 10.06 VVGGLDD271 pKa = 3.6 NRR273 pKa = 11.84 VSPYY277 pKa = 9.64 TGNSGDD283 pKa = 4.18 TPTMQCDD290 pKa = 3.55 MLQLKK295 pKa = 9.31 PGRR298 pKa = 11.84 YY299 pKa = 8.55 SVRR302 pKa = 11.84 SSPRR306 pKa = 11.84 FLLMPEE312 pKa = 4.1 RR313 pKa = 11.84 SYY315 pKa = 11.73 CFDD318 pKa = 3.5 MKK320 pKa = 10.99 EE321 pKa = 4.16 KK322 pKa = 11.04 GPVTAVQSIWGKK334 pKa = 10.35 GRR336 pKa = 11.84 EE337 pKa = 4.07 SDD339 pKa = 3.62 HH340 pKa = 7.93 AVDD343 pKa = 4.18 QACLSTPGCMLIQKK357 pKa = 7.37 QKK359 pKa = 10.35 PYY361 pKa = 10.24 IGEE364 pKa = 4.33 ADD366 pKa = 3.72 DD367 pKa = 3.83 HH368 pKa = 7.68 HH369 pKa = 7.57 GDD371 pKa = 3.26 QEE373 pKa = 4.19 MRR375 pKa = 11.84 EE376 pKa = 4.26 LLSGLDD382 pKa = 3.52 YY383 pKa = 10.79 EE384 pKa = 5.02 ARR386 pKa = 11.84 CISQSGWVNEE396 pKa = 4.0 TSPFTEE402 pKa = 5.12 EE403 pKa = 3.88 YY404 pKa = 10.82 LLPPKK409 pKa = 10.63 FGRR412 pKa = 11.84 CPLAAKK418 pKa = 10.04 EE419 pKa = 3.87 EE420 pKa = 4.69 SIPKK424 pKa = 9.95 IPDD427 pKa = 3.22 GLLIPTSGTDD437 pKa = 3.31 TTVTKK442 pKa = 10.09 PKK444 pKa = 10.63 SRR446 pKa = 11.84 IFGIDD451 pKa = 3.38 DD452 pKa = 5.09 LIIGLLFVAIVEE464 pKa = 4.27 AGIGGYY470 pKa = 10.53 LLGSRR475 pKa = 11.84 KK476 pKa = 9.78 VSGGGVTKK484 pKa = 10.59 EE485 pKa = 3.9 SAEE488 pKa = 4.1 KK489 pKa = 10.63 GFEE492 pKa = 4.3 KK493 pKa = 10.09 IGNDD497 pKa = 3.11 IQILRR502 pKa = 11.84 SSTNIAIEE510 pKa = 4.02 KK511 pKa = 9.93 LNDD514 pKa = 4.38 RR515 pKa = 11.84 ISHH518 pKa = 6.87 DD519 pKa = 3.58 EE520 pKa = 3.67 QAIRR524 pKa = 11.84 DD525 pKa = 3.81 LTLEE529 pKa = 4.02 IEE531 pKa = 4.48 NARR534 pKa = 11.84 SEE536 pKa = 4.27 ALLGEE541 pKa = 4.29 LGIIRR546 pKa = 11.84 ALLVGNISIGLQEE559 pKa = 4.78 SLWEE563 pKa = 3.97 LASEE567 pKa = 4.14 ITNRR571 pKa = 11.84 AGDD574 pKa = 3.74 LAVEE578 pKa = 4.25 VSPGCWVIDD587 pKa = 4.49 NNICDD592 pKa = 3.64 QSCQNFIFKK601 pKa = 10.49 FNEE604 pKa = 3.92 TAPVPTIPPLDD615 pKa = 3.72 TKK617 pKa = 10.71 IDD619 pKa = 3.76 LQSDD623 pKa = 4.09 PFYY626 pKa = 10.2 WGSSLGLAITAAISLAALVISGIAICRR653 pKa = 11.84 TKK655 pKa = 11.06
Molecular weight: 71.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.594
IPC2_protein 5.715
IPC_protein 5.728
Toseland 5.881
ProMoST 6.033
Dawson 5.931
Bjellqvist 5.97
Wikipedia 5.906
Rodwell 5.893
Grimsley 5.931
Solomon 5.931
Lehninger 5.919
Nozaki 6.173
DTASelect 6.338
Thurlkill 6.3
EMBOSS 6.262
Sillero 6.262
Patrickios 3.986
IPC_peptide 5.944
IPC2_peptide 6.224
IPC2.peptide.svr19 6.291
Protein with the highest isoelectric point:
>sp|Q6I7C2|PA_INCAA Polymerase acidic protein OS=Influenza C virus (strain C/Ann Arbor/1/1950) OX=11553 GN=PA PE=3 SV=1
MM1 pKa = 7.83 SDD3 pKa = 2.89 RR4 pKa = 11.84 RR5 pKa = 11.84 QNRR8 pKa = 11.84 KK9 pKa = 7.56 TPDD12 pKa = 3.24 EE13 pKa = 3.94 QRR15 pKa = 11.84 KK16 pKa = 9.78 ANALIINEE24 pKa = 4.26 NIEE27 pKa = 4.08 AYY29 pKa = 9.61 IAICKK34 pKa = 9.53 EE35 pKa = 3.66 VGLNGDD41 pKa = 3.56 EE42 pKa = 4.26 MLILEE47 pKa = 4.5 NGIAIEE53 pKa = 3.93 KK54 pKa = 10.08 AIRR57 pKa = 11.84 ICCDD61 pKa = 2.38 GKK63 pKa = 10.13 YY64 pKa = 9.29 QEE66 pKa = 4.64 KK67 pKa = 10.08 RR68 pKa = 11.84 EE69 pKa = 4.12 KK70 pKa = 10.04 KK71 pKa = 9.88 ARR73 pKa = 11.84 EE74 pKa = 3.88 AQRR77 pKa = 11.84 ADD79 pKa = 3.47 SNFNADD85 pKa = 3.52 SIGIRR90 pKa = 11.84 LVKK93 pKa = 10.35 RR94 pKa = 11.84 AGSGTNITYY103 pKa = 9.98 HH104 pKa = 6.27 AVVEE108 pKa = 4.27 LTSRR112 pKa = 11.84 SRR114 pKa = 11.84 IVQILKK120 pKa = 8.9 SHH122 pKa = 7.09 WGNEE126 pKa = 3.72 LNRR129 pKa = 11.84 AKK131 pKa = 10.48 IAGKK135 pKa = 10.23 RR136 pKa = 11.84 LGFSALFASNLEE148 pKa = 4.16 AIIYY152 pKa = 9.11 QRR154 pKa = 11.84 GRR156 pKa = 11.84 NAARR160 pKa = 11.84 RR161 pKa = 11.84 NGSAEE166 pKa = 4.46 LFTLTQGAGIEE177 pKa = 4.19 TRR179 pKa = 11.84 YY180 pKa = 9.47 KK181 pKa = 10.06 WIMEE185 pKa = 3.75 KK186 pKa = 10.51 HH187 pKa = 5.69 IGIGVLIADD196 pKa = 4.58 AKK198 pKa = 11.06 GLINGKK204 pKa = 9.56 RR205 pKa = 11.84 EE206 pKa = 3.92 GKK208 pKa = 10.23 RR209 pKa = 11.84 GVDD212 pKa = 3.21 ANVKK216 pKa = 9.73 LRR218 pKa = 11.84 AGTTGSPLEE227 pKa = 4.09 RR228 pKa = 11.84 AMQGIEE234 pKa = 3.93 KK235 pKa = 10.25 KK236 pKa = 10.62 AFPGPLRR243 pKa = 11.84 ALARR247 pKa = 11.84 RR248 pKa = 11.84 VVKK251 pKa = 10.75 ANYY254 pKa = 9.66 NDD256 pKa = 3.09 ARR258 pKa = 11.84 EE259 pKa = 4.19 ALNVIAEE266 pKa = 4.22 ASLLLKK272 pKa = 10.13 PQITNKK278 pKa = 7.67 MTMPWCMWLAARR290 pKa = 11.84 LTLKK294 pKa = 11.01 DD295 pKa = 3.52 EE296 pKa = 4.41 FANFCAYY303 pKa = 10.19 AGRR306 pKa = 11.84 RR307 pKa = 11.84 AFEE310 pKa = 4.07 VFNIAMEE317 pKa = 4.64 KK318 pKa = 10.09 IGICSFQGTIMNDD331 pKa = 3.39 DD332 pKa = 4.45 EE333 pKa = 5.4 IEE335 pKa = 4.41 SIEE338 pKa = 4.93 DD339 pKa = 3.31 KK340 pKa = 11.27 AQVLMMACFGLAYY353 pKa = 9.94 EE354 pKa = 4.84 DD355 pKa = 4.71 FSLVSAMVSHH365 pKa = 7.32 PLKK368 pKa = 10.38 LRR370 pKa = 11.84 NRR372 pKa = 11.84 MKK374 pKa = 10.24 IGNFRR379 pKa = 11.84 VGEE382 pKa = 4.17 KK383 pKa = 10.52 VSTVLSPLLRR393 pKa = 11.84 FTRR396 pKa = 11.84 WAEE399 pKa = 3.67 FAQRR403 pKa = 11.84 FALQANTSRR412 pKa = 11.84 EE413 pKa = 3.87 GAQISNSAVFAVEE426 pKa = 4.41 RR427 pKa = 11.84 KK428 pKa = 8.24 ITTDD432 pKa = 3.15 VQRR435 pKa = 11.84 VEE437 pKa = 4.06 EE438 pKa = 4.46 LLNKK442 pKa = 8.94 VQAHH446 pKa = 5.9 EE447 pKa = 4.75 DD448 pKa = 3.71 EE449 pKa = 4.97 PLQTLYY455 pKa = 11.16 KK456 pKa = 9.96 KK457 pKa = 10.59 VRR459 pKa = 11.84 EE460 pKa = 4.02 QISIIGRR467 pKa = 11.84 NKK469 pKa = 10.34 SEE471 pKa = 3.73 IKK473 pKa = 10.41 EE474 pKa = 4.03 FLGSSMYY481 pKa = 10.88 DD482 pKa = 3.13 LNDD485 pKa = 3.36 QEE487 pKa = 4.62 KK488 pKa = 10.41 QNPINFRR495 pKa = 11.84 SGAHH499 pKa = 5.88 PFFFEE504 pKa = 5.07 FDD506 pKa = 3.53 PDD508 pKa = 3.82 YY509 pKa = 11.6 NPIRR513 pKa = 11.84 VKK515 pKa = 10.61 RR516 pKa = 11.84 PKK518 pKa = 10.57 KK519 pKa = 10.15 PIAKK523 pKa = 9.26 RR524 pKa = 11.84 NSNISRR530 pKa = 11.84 LEE532 pKa = 3.86 EE533 pKa = 3.73 EE534 pKa = 5.15 GMDD537 pKa = 3.66 EE538 pKa = 4.51 NSEE541 pKa = 3.74 IGQAKK546 pKa = 9.44 KK547 pKa = 9.97 MKK549 pKa = 9.96 PLDD552 pKa = 3.68 QLTSTSSNIPGKK564 pKa = 10.28 NN565 pKa = 3.02
Molecular weight: 63.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.922
IPC2_protein 8.858
IPC_protein 8.77
Toseland 9.75
ProMoST 9.355
Dawson 9.926
Bjellqvist 9.589
Wikipedia 10.058
Rodwell 10.394
Grimsley 9.97
Solomon 9.955
Lehninger 9.926
Nozaki 9.823
DTASelect 9.545
Thurlkill 9.794
EMBOSS 10.16
Sillero 9.867
Patrickios 9.867
IPC_peptide 9.955
IPC2_peptide 8.097
IPC2.peptide.svr19 7.794
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
1
9
4501
182
774
500.1
56.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.91 ± 0.553
2.155 ± 0.236
4.754 ± 0.191
7.976 ± 0.496
4.177 ± 0.409
6.043 ± 0.472
1.4 ± 0.149
7.087 ± 0.323
8.443 ± 0.438
8.643 ± 0.425
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.955 ± 0.508
4.91 ± 0.326
3.866 ± 0.252
2.977 ± 0.304
5.643 ± 0.506
6.976 ± 0.512
5.554 ± 0.436
4.888 ± 0.336
1.178 ± 0.08
2.466 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here