Faeces associated gemycircularvirus 11
Average proteome isoelectric point is 7.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T1YTA5|T1YTA5_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 11 OX=1391026 PE=3 SV=1
MM1 pKa = 7.28 LVARR5 pKa = 11.84 SSVPPRR11 pKa = 11.84 VMPRR15 pKa = 11.84 TVNWSVALEE24 pKa = 4.08 SVAFLTYY31 pKa = 10.22 SQYY34 pKa = 11.4 GPADD38 pKa = 3.63 GVSDD42 pKa = 3.67 IQASSQCFEE51 pKa = 4.33 YY52 pKa = 10.33 IIILVAHH59 pKa = 6.59 EE60 pKa = 3.85 RR61 pKa = 11.84 HH62 pKa = 6.34 EE63 pKa = 5.04 DD64 pKa = 3.63 GGHH67 pKa = 6.33 HH68 pKa = 5.23 YY69 pKa = 10.14 HH70 pKa = 7.05 VLVAWEE76 pKa = 4.33 SHH78 pKa = 5.41 FKK80 pKa = 11.21 ANDD83 pKa = 2.96 VRR85 pKa = 11.84 IFDD88 pKa = 4.71 VGGCHH93 pKa = 7.07 PNFKK97 pKa = 10.26 SVRR100 pKa = 11.84 GDD102 pKa = 3.39 GNINRR107 pKa = 11.84 VLEE110 pKa = 4.06 QLRR113 pKa = 11.84 LAQFLPGYY121 pKa = 8.35 CLKK124 pKa = 11.18 DD125 pKa = 2.95 GDD127 pKa = 4.57 YY128 pKa = 11.31 YY129 pKa = 11.48 GDD131 pKa = 3.6 PEE133 pKa = 4.49 VFRR136 pKa = 11.84 RR137 pKa = 11.84 DD138 pKa = 3.29 APKK141 pKa = 10.43 RR142 pKa = 11.84 SQTSIWTEE150 pKa = 3.66 IVSAEE155 pKa = 4.09 SAGQFLQLARR165 pKa = 11.84 EE166 pKa = 4.41 LAPKK170 pKa = 10.16 EE171 pKa = 3.68 YY172 pKa = 10.49 GLNYY176 pKa = 10.01 DD177 pKa = 4.39 RR178 pKa = 11.84 LVSYY182 pKa = 10.69 CRR184 pKa = 11.84 AHH186 pKa = 6.0 YY187 pKa = 8.54 DD188 pKa = 3.09 KK189 pKa = 10.6 PAEE192 pKa = 4.01 YY193 pKa = 10.53 VPRR196 pKa = 11.84 HH197 pKa = 5.32 VDD199 pKa = 3.7 FVVPSEE205 pKa = 3.95 LDD207 pKa = 2.9 DD208 pKa = 3.72 WVNVNVSNAAPEE220 pKa = 4.11 QPEE223 pKa = 4.22 RR224 pKa = 11.84 PRR226 pKa = 11.84 ALCLIGRR233 pKa = 11.84 SRR235 pKa = 11.84 LGKK238 pKa = 7.97 TEE240 pKa = 3.82 WARR243 pKa = 11.84 SLGKK247 pKa = 9.77 HH248 pKa = 5.98 SYY250 pKa = 9.45 FASLFNIDD258 pKa = 5.24 DD259 pKa = 4.05 FDD261 pKa = 5.38 GSADD265 pKa = 3.76 YY266 pKa = 11.48 AVMDD270 pKa = 5.72 DD271 pKa = 3.68 IDD273 pKa = 4.32 IKK275 pKa = 10.89 FFPNYY280 pKa = 9.54 KK281 pKa = 9.9 GWFGAQKK288 pKa = 10.17 RR289 pKa = 11.84 FCITGKK295 pKa = 8.46 YY296 pKa = 8.94 ARR298 pKa = 11.84 HH299 pKa = 5.49 RR300 pKa = 11.84 TVDD303 pKa = 2.85 WGKK306 pKa = 10.08 PMIWCTNDD314 pKa = 3.66 DD315 pKa = 3.4 PRR317 pKa = 11.84 QAVGIDD323 pKa = 3.29 QDD325 pKa = 3.81 WFSEE329 pKa = 4.14 NPDD332 pKa = 3.35 FVIVQEE338 pKa = 4.07 PFLLGSLQVALALATDD354 pKa = 4.06 SPRR357 pKa = 11.84 FDD359 pKa = 3.36 SS360 pKa = 4.38
Molecular weight: 40.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.36
IPC2_protein 5.347
IPC_protein 5.359
Toseland 5.614
ProMoST 5.626
Dawson 5.512
Bjellqvist 5.575
Wikipedia 5.486
Rodwell 5.474
Grimsley 5.703
Solomon 5.512
Lehninger 5.499
Nozaki 5.728
DTASelect 5.931
Thurlkill 5.804
EMBOSS 5.779
Sillero 5.817
Patrickios 3.884
IPC_peptide 5.525
IPC2_peptide 5.83
IPC2.peptide.svr19 5.778
Protein with the highest isoelectric point:
>tr|T1YTA5|T1YTA5_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 11 OX=1391026 PE=3 SV=1
MM1 pKa = 7.47 PRR3 pKa = 11.84 YY4 pKa = 9.69 AKK6 pKa = 9.26 RR7 pKa = 11.84 TSKK10 pKa = 10.26 KK11 pKa = 9.54 RR12 pKa = 11.84 PAYY15 pKa = 9.56 RR16 pKa = 11.84 KK17 pKa = 8.54 KK18 pKa = 10.17 AAYY21 pKa = 9.46 RR22 pKa = 11.84 GKK24 pKa = 10.41 KK25 pKa = 8.02 RR26 pKa = 11.84 TYY28 pKa = 9.94 SKK30 pKa = 9.66 KK31 pKa = 9.03 TSSSLAKK38 pKa = 10.01 KK39 pKa = 9.2 IHH41 pKa = 6.18 AVADD45 pKa = 4.04 TKK47 pKa = 11.23 KK48 pKa = 10.45 SDD50 pKa = 3.3 TMACAIAAPVTGANPSGVEE69 pKa = 3.96 VKK71 pKa = 10.73 EE72 pKa = 4.19 FMDD75 pKa = 3.96 WPLDD79 pKa = 3.9 SQIKK83 pKa = 9.58 IYY85 pKa = 9.3 MWAPLYY91 pKa = 10.65 RR92 pKa = 11.84 VGTDD96 pKa = 2.54 KK97 pKa = 11.08 KK98 pKa = 11.04 GPHH101 pKa = 6.39 LRR103 pKa = 11.84 NASRR107 pKa = 11.84 VHH109 pKa = 4.41 FTGVSEE115 pKa = 4.38 RR116 pKa = 11.84 MNIASNSRR124 pKa = 11.84 VPFRR128 pKa = 11.84 HH129 pKa = 5.44 RR130 pKa = 11.84 RR131 pKa = 11.84 LVIRR135 pKa = 11.84 AAVDD139 pKa = 3.69 FTSRR143 pKa = 11.84 MLASKK148 pKa = 10.74 ADD150 pKa = 3.53 PTLVFRR156 pKa = 11.84 NLDD159 pKa = 3.48 QLTPTALDD167 pKa = 3.78 TYY169 pKa = 11.26 FLGQKK174 pKa = 9.66 NLDD177 pKa = 3.15 WSDD180 pKa = 4.13 PIKK183 pKa = 11.09 ADD185 pKa = 3.29 VDD187 pKa = 3.89 KK188 pKa = 11.41 QQLTVLFDD196 pKa = 3.05 KK197 pKa = 10.63 TYY199 pKa = 10.37 TYY201 pKa = 11.22 NPPNDD206 pKa = 3.85 FGLDD210 pKa = 3.51 STRR213 pKa = 11.84 TSITRR218 pKa = 11.84 LTAKK222 pKa = 10.33 VGLPTRR228 pKa = 11.84 KK229 pKa = 10.21 DD230 pKa = 3.38 GVNTKK235 pKa = 8.51 WSAKK239 pKa = 9.99 IGTKK243 pKa = 9.9 EE244 pKa = 3.96 PNDD247 pKa = 3.51 VVIIDD252 pKa = 4.16 MFRR255 pKa = 11.84 SNYY258 pKa = 7.46 DD259 pKa = 2.8 TRR261 pKa = 11.84 TNTFPLGIEE270 pKa = 3.81 SWTIGSKK277 pKa = 10.52 CKK279 pKa = 10.45 GYY281 pKa = 11.14 LEE283 pKa = 4.49 GTT285 pKa = 3.83
Molecular weight: 32.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.171
IPC2_protein 9.487
IPC_protein 9.531
Toseland 10.321
ProMoST 9.897
Dawson 10.452
Bjellqvist 10.087
Wikipedia 10.599
Rodwell 10.994
Grimsley 10.511
Solomon 10.482
Lehninger 10.452
Nozaki 10.292
DTASelect 10.072
Thurlkill 10.321
EMBOSS 10.701
Sillero 10.365
Patrickios 10.672
IPC_peptide 10.482
IPC2_peptide 8.624
IPC2.peptide.svr19 8.568
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
645
285
360
322.5
36.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.062 ± 0.005
1.395 ± 0.427
7.752 ± 0.452
3.721 ± 0.995
4.651 ± 0.703
5.891 ± 0.387
2.326 ± 0.568
4.496 ± 0.04
6.512 ± 2.256
6.977 ± 0.191
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.705 ± 0.462
3.876 ± 0.206
5.736 ± 0.075
2.946 ± 0.734
7.287 ± 0.266
7.287 ± 0.05
5.736 ± 2.517
7.287 ± 0.814
2.171 ± 0.256
4.186 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here