Citreimonas salinaria
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4109 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H3M1A7|A0A1H3M1A7_9RHOB Aminobenzoyl-glutamate utilization protein B OS=Citreimonas salinaria OX=321339 GN=SAMN05444340_11548 PE=4 SV=1
MM1 pKa = 7.57 KK2 pKa = 10.28 SILFATSVLVASAGMASAQYY22 pKa = 11.1 VDD24 pKa = 3.6 VAGSAEE30 pKa = 3.92 VGIVGGEE37 pKa = 3.9 RR38 pKa = 11.84 YY39 pKa = 7.61 GTPSSGGVDD48 pKa = 3.54 QIFTDD53 pKa = 3.62 IEE55 pKa = 4.66 VVFTMTGQTDD65 pKa = 3.01 NGLSYY70 pKa = 10.66 GASVDD75 pKa = 4.12 LDD77 pKa = 3.9 EE78 pKa = 7.35 GDD80 pKa = 4.98 DD81 pKa = 4.2 SFDD84 pKa = 3.58 GTDD87 pKa = 4.16 DD88 pKa = 5.83 DD89 pKa = 4.52 GATWFLVYY97 pKa = 10.58 GDD99 pKa = 4.15 YY100 pKa = 10.97 RR101 pKa = 11.84 LDD103 pKa = 3.7 VGDD106 pKa = 3.7 TDD108 pKa = 4.19 GAFDD112 pKa = 3.72 AAVAEE117 pKa = 4.37 TSLVGTINDD126 pKa = 3.98 TEE128 pKa = 4.08 EE129 pKa = 3.93 HH130 pKa = 6.22 LGYY133 pKa = 10.33 SGNSALDD140 pKa = 4.17 GIYY143 pKa = 10.41 DD144 pKa = 3.72 GQIARR149 pKa = 11.84 LSRR152 pKa = 11.84 SAGSVAGHH160 pKa = 5.88 VSVEE164 pKa = 3.87 VDD166 pKa = 4.12 DD167 pKa = 4.64 MGEE170 pKa = 3.93 SSPVYY175 pKa = 10.21 GVGFTYY181 pKa = 10.43 DD182 pKa = 3.05 RR183 pKa = 11.84 RR184 pKa = 11.84 IAGVDD189 pKa = 3.16 VRR191 pKa = 11.84 VGVGHH196 pKa = 6.08 QRR198 pKa = 11.84 TVVDD202 pKa = 3.65 ADD204 pKa = 4.54 DD205 pKa = 4.01 YY206 pKa = 11.97 GFDD209 pKa = 4.63 DD210 pKa = 5.01 GLHH213 pKa = 5.14 TVAGASVSTSHH224 pKa = 6.69 EE225 pKa = 3.91 NGLRR229 pKa = 11.84 TVASYY234 pKa = 11.5 VEE236 pKa = 4.05 YY237 pKa = 10.93 DD238 pKa = 3.23 QANWSKK244 pKa = 9.48 TMKK247 pKa = 10.21 HH248 pKa = 5.16 SAIGVGYY255 pKa = 8.36 TRR257 pKa = 11.84 DD258 pKa = 3.82 ALSVAANYY266 pKa = 10.17 GIYY269 pKa = 10.41 NDD271 pKa = 3.91 FDD273 pKa = 4.37 GVDD276 pKa = 3.69 GPTSRR281 pKa = 11.84 GYY283 pKa = 11.4 AFAVNYY289 pKa = 10.17 DD290 pKa = 3.56 LGGGLVAQAGFSEE303 pKa = 4.67 SDD305 pKa = 3.39 LKK307 pKa = 11.45 VDD309 pKa = 3.93 GFEE312 pKa = 4.89 HH313 pKa = 7.04 EE314 pKa = 4.37 GDD316 pKa = 3.65 NYY318 pKa = 11.12 SLGVAMAFF326 pKa = 3.68
Molecular weight: 34.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.834
IPC_protein 3.872
Toseland 3.63
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.821
Rodwell 3.694
Grimsley 3.541
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.266
Thurlkill 3.694
EMBOSS 3.834
Sillero 3.986
Patrickios 1.049
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.861
Protein with the highest isoelectric point:
>tr|A0A1H3KPV1|A0A1H3KPV1_9RHOB dCTP deaminase OS=Citreimonas salinaria OX=321339 GN=SAMN05444340_11037 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4109
0
4109
1253694
30
2143
305.1
33.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.073 ± 0.057
0.862 ± 0.012
6.299 ± 0.034
5.981 ± 0.037
3.559 ± 0.024
8.785 ± 0.039
2.108 ± 0.018
4.807 ± 0.029
2.583 ± 0.03
10.078 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.707 ± 0.017
2.227 ± 0.023
5.258 ± 0.025
2.991 ± 0.019
7.588 ± 0.04
4.864 ± 0.026
5.343 ± 0.024
7.395 ± 0.036
1.425 ± 0.017
2.066 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here