Bemisia-associated genomovirus AdDF

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemyduguivirus; Gemyduguivirus bemta1

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z2X294|A0A1Z2X294_9VIRU Replication-associated protein OS=Bemisia-associated genomovirus AdDF OX=1986477 GN=Rep PE=3 SV=2
MM1 pKa = 7.45TFRR4 pKa = 11.84FAAKK8 pKa = 10.31YY9 pKa = 10.64GLLTYY14 pKa = 10.01AQSDD18 pKa = 3.96GLDD21 pKa = 3.4PLSIVNHH28 pKa = 6.38LGDD31 pKa = 5.01LGAEE35 pKa = 4.19CIVGRR40 pKa = 11.84EE41 pKa = 4.07LHH43 pKa = 7.3DD44 pKa = 4.97DD45 pKa = 4.01GGTHH49 pKa = 4.94YY50 pKa = 10.57HH51 pKa = 6.36AFFMFEE57 pKa = 4.62KK58 pKa = 10.45KK59 pKa = 9.96FQSRR63 pKa = 11.84NARR66 pKa = 11.84VFDD69 pKa = 3.53IDD71 pKa = 4.14SYY73 pKa = 11.35HH74 pKa = 6.64PNILRR79 pKa = 11.84GIKK82 pKa = 9.11TPEE85 pKa = 3.68SMYY88 pKa = 11.01DD89 pKa = 3.44YY90 pKa = 9.52ATKK93 pKa = 10.8DD94 pKa = 3.11GDD96 pKa = 3.49ICGGGLEE103 pKa = 4.42RR104 pKa = 11.84PDD106 pKa = 3.64PRR108 pKa = 11.84GEE110 pKa = 3.98SSKK113 pKa = 11.39NNDD116 pKa = 3.49DD117 pKa = 3.28FWRR120 pKa = 11.84GVFDD124 pKa = 5.02AEE126 pKa = 4.67GKK128 pKa = 10.57EE129 pKa = 3.97EE130 pKa = 4.05TLRR133 pKa = 11.84CARR136 pKa = 11.84EE137 pKa = 3.84LGVGLFGRR145 pKa = 11.84YY146 pKa = 7.89YY147 pKa = 9.51FQVRR151 pKa = 11.84AIAEE155 pKa = 4.17EE156 pKa = 4.03KK157 pKa = 10.62ARR159 pKa = 11.84RR160 pKa = 11.84SPLDD164 pKa = 3.49YY165 pKa = 11.06KK166 pKa = 10.91PDD168 pKa = 4.4DD169 pKa = 3.7SLEE172 pKa = 4.26WEE174 pKa = 4.39LGPYY178 pKa = 9.79PEE180 pKa = 4.55LTDD183 pKa = 3.05WCASYY188 pKa = 10.71LGRR191 pKa = 11.84RR192 pKa = 11.84GGRR195 pKa = 11.84PRR197 pKa = 11.84SLVLVGPSRR206 pKa = 11.84TGKK209 pKa = 6.11TTWARR214 pKa = 11.84CLGGHH219 pKa = 6.38FFFGGNFDD227 pKa = 3.97MGQLIHH233 pKa = 7.43DD234 pKa = 4.8CDD236 pKa = 4.76DD237 pKa = 2.96IKK239 pKa = 11.45YY240 pKa = 10.5AIFDD244 pKa = 4.08DD245 pKa = 4.33MPGLKK250 pKa = 10.18YY251 pKa = 10.71FPTWKK256 pKa = 9.97FWLGAQDD263 pKa = 3.71TFTVTDD269 pKa = 3.67KK270 pKa = 11.56YY271 pKa = 10.29KK272 pKa = 11.38GKK274 pKa = 8.68MCFDD278 pKa = 3.93WGRR281 pKa = 11.84PVIWCNNRR289 pKa = 11.84DD290 pKa = 3.65PRR292 pKa = 11.84EE293 pKa = 4.37DD294 pKa = 3.58PDD296 pKa = 6.04ADD298 pKa = 4.67VDD300 pKa = 4.0WLDD303 pKa = 3.86ANCIFVNVPADD314 pKa = 3.52YY315 pKa = 10.73PLIKK319 pKa = 10.34QQ320 pKa = 3.54

Molecular weight: 36.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z2X294|A0A1Z2X294_9VIRU Replication-associated protein OS=Bemisia-associated genomovirus AdDF OX=1986477 GN=Rep PE=3 SV=2
MM1 pKa = 7.78PFRR4 pKa = 11.84RR5 pKa = 11.84SHH7 pKa = 6.01ARR9 pKa = 11.84STRR12 pKa = 11.84YY13 pKa = 9.42RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84TTRR19 pKa = 11.84RR20 pKa = 11.84VTRR23 pKa = 11.84RR24 pKa = 11.84PARR27 pKa = 11.84RR28 pKa = 11.84TITRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84PTRR37 pKa = 11.84NGYY40 pKa = 8.15NSRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84ILNIASTKK53 pKa = 10.09KK54 pKa = 9.53QDD56 pKa = 3.21NMLPVVAANDD66 pKa = 4.0GTSTVPGPLTINGNQPMMLLWCATARR92 pKa = 11.84DD93 pKa = 3.71RR94 pKa = 11.84VSNVGRR100 pKa = 11.84DD101 pKa = 3.36NASSVRR107 pKa = 11.84EE108 pKa = 3.75SDD110 pKa = 3.48LVYY113 pKa = 10.33MRR115 pKa = 11.84GLKK118 pKa = 10.12EE119 pKa = 4.38KK120 pKa = 10.71INITTTTQASWHH132 pKa = 4.84WRR134 pKa = 11.84RR135 pKa = 11.84ICFTAKK141 pKa = 10.01GVNQFLTALDD151 pKa = 3.87SLEE154 pKa = 4.4TSAGWVRR161 pKa = 11.84LLTNINNTTSGNNILGYY178 pKa = 8.99VFKK181 pKa = 10.16GTQGVDD187 pKa = 2.35WYY189 pKa = 10.59DD190 pKa = 3.21AFTAKK195 pKa = 10.25VDD197 pKa = 3.73TNRR200 pKa = 11.84VSIKK204 pKa = 10.36YY205 pKa = 9.43DD206 pKa = 3.0QTRR209 pKa = 11.84NFSSGNAQGRR219 pKa = 11.84YY220 pKa = 8.71FKK222 pKa = 10.67HH223 pKa = 6.51KK224 pKa = 9.95NWFPMNQNLMYY235 pKa = 10.87SNDD238 pKa = 3.48EE239 pKa = 4.29AGEE242 pKa = 4.25SEE244 pKa = 5.02SNDD247 pKa = 3.12NHH249 pKa = 5.85STLGRR254 pKa = 11.84AGMGDD259 pKa = 4.28YY260 pKa = 10.84YY261 pKa = 11.59VLDD264 pKa = 5.63FITCATNQSADD275 pKa = 3.26TMLFTPEE282 pKa = 3.48ATLYY286 pKa = 8.97WHH288 pKa = 7.08EE289 pKa = 4.26KK290 pKa = 9.3

Molecular weight: 33.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

610

290

320

305.0

34.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.393 ± 0.356

1.967 ± 0.66

8.033 ± 2.024

3.934 ± 0.832

5.082 ± 1.156

8.361 ± 1.524

1.967 ± 0.172

3.934 ± 0.1

4.59 ± 0.564

7.049 ± 0.596

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.459 ± 0.456

5.902 ± 2.168

4.426 ± 0.936

2.459 ± 0.456

9.016 ± 1.428

5.41 ± 1.296

7.541 ± 2.472

4.59 ± 0.412

2.623 ± 0.148

4.262 ± 0.332
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski