Sida yellow vein virus satellite DNA beta
Average proteome isoelectric point is 9.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4GZI2|Q4GZI2_9VIRU C1 protein OS=Sida yellow vein virus satellite DNA beta OX=326489 GN=c1 PE=4 SV=1
MM1 pKa = 7.12 TIKK4 pKa = 10.61 YY5 pKa = 9.66 KK6 pKa = 10.38 NRR8 pKa = 11.84 KK9 pKa = 7.92 GLEE12 pKa = 4.19 FIIDD16 pKa = 3.68 VQVEE20 pKa = 4.12 GGRR23 pKa = 11.84 FYY25 pKa = 10.96 RR26 pKa = 11.84 SSAEE30 pKa = 3.61 AVLQHH35 pKa = 5.78 GHH37 pKa = 6.46 KK38 pKa = 9.59 CCQGGTSSIPIRR50 pKa = 11.84 IRR52 pKa = 11.84 KK53 pKa = 6.24 GHH55 pKa = 6.02 RR56 pKa = 11.84 AVRR59 pKa = 11.84 LQFLGGGDD67 pKa = 3.59 KK68 pKa = 10.66 EE69 pKa = 4.65 HH70 pKa = 6.99 IRR72 pKa = 11.84 HH73 pKa = 5.84 HH74 pKa = 6.15 VPGNRR79 pKa = 11.84 LSTEE83 pKa = 3.9 FQAGGRR89 pKa = 11.84 GGGNRR94 pKa = 11.84 YY95 pKa = 8.67 PHH97 pKa = 7.29 DD98 pKa = 3.89 PRR100 pKa = 11.84 GKK102 pKa = 8.84 GRR104 pKa = 11.84 GYY106 pKa = 10.33 RR107 pKa = 11.84 PCNRR111 pKa = 11.84 IRR113 pKa = 11.84 CIYY116 pKa = 10.35 ALIRR120 pKa = 11.84 ISAA123 pKa = 3.71
Molecular weight: 13.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.867
IPC_protein 10.862
Toseland 10.877
ProMoST 10.745
Dawson 10.965
Bjellqvist 10.745
Wikipedia 11.228
Rodwell 11.067
Grimsley 11.008
Solomon 11.155
Lehninger 11.096
Nozaki 10.877
DTASelect 10.73
Thurlkill 10.877
EMBOSS 11.301
Sillero 10.906
Patrickios 10.789
IPC_peptide 11.155
IPC2_peptide 10.028
IPC2.peptide.svr19 8.399
Protein with the highest isoelectric point:
>tr|Q4GZI2|Q4GZI2_9VIRU C1 protein OS=Sida yellow vein virus satellite DNA beta OX=326489 GN=c1 PE=4 SV=1
MM1 pKa = 7.12 TIKK4 pKa = 10.61 YY5 pKa = 9.66 KK6 pKa = 10.38 NRR8 pKa = 11.84 KK9 pKa = 7.92 GLEE12 pKa = 4.19 FIIDD16 pKa = 3.68 VQVEE20 pKa = 4.12 GGRR23 pKa = 11.84 FYY25 pKa = 10.96 RR26 pKa = 11.84 SSAEE30 pKa = 3.61 AVLQHH35 pKa = 5.78 GHH37 pKa = 6.46 KK38 pKa = 9.59 CCQGGTSSIPIRR50 pKa = 11.84 IRR52 pKa = 11.84 KK53 pKa = 6.24 GHH55 pKa = 6.02 RR56 pKa = 11.84 AVRR59 pKa = 11.84 LQFLGGGDD67 pKa = 3.59 KK68 pKa = 10.66 EE69 pKa = 4.65 HH70 pKa = 6.99 IRR72 pKa = 11.84 HH73 pKa = 5.84 HH74 pKa = 6.15 VPGNRR79 pKa = 11.84 LSTEE83 pKa = 3.9 FQAGGRR89 pKa = 11.84 GGGNRR94 pKa = 11.84 YY95 pKa = 8.67 PHH97 pKa = 7.29 DD98 pKa = 3.89 PRR100 pKa = 11.84 GKK102 pKa = 8.84 GRR104 pKa = 11.84 GYY106 pKa = 10.33 RR107 pKa = 11.84 PCNRR111 pKa = 11.84 IRR113 pKa = 11.84 CIYY116 pKa = 10.35 ALIRR120 pKa = 11.84 ISAA123 pKa = 3.71
Molecular weight: 13.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.867
IPC_protein 10.862
Toseland 10.877
ProMoST 10.745
Dawson 10.965
Bjellqvist 10.745
Wikipedia 11.228
Rodwell 11.067
Grimsley 11.008
Solomon 11.155
Lehninger 11.096
Nozaki 10.877
DTASelect 10.73
Thurlkill 10.877
EMBOSS 11.301
Sillero 10.906
Patrickios 10.789
IPC_peptide 11.155
IPC2_peptide 10.028
IPC2.peptide.svr19 8.399
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
123
123
123
123.0
13.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.878 ± 0.0
3.252 ± 0.0
2.439 ± 0.0
4.065 ± 0.0
3.252 ± 0.0
15.447 ± 0.0
5.691 ± 0.0
8.943 ± 0.0
5.691 ± 0.0
4.878 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
0.813 ± 0.0
3.252 ± 0.0
4.065 ± 0.0
4.065 ± 0.0
13.821 ± 0.0
4.878 ± 0.0
2.439 ± 0.0
4.065 ± 0.0
0.0 ± 0.0
4.065 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here