Bufonid herpesvirus 1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; unclassified Herpesvirales

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 152 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A384ZV31|A0A384ZV31_9VIRU Allene oxide cyclase-like protein OS=Bufonid herpesvirus 1 OX=2282206 PE=4 SV=1
MM1 pKa = 7.58SGTDD5 pKa = 4.26AIDD8 pKa = 3.49ALGDD12 pKa = 3.65FEE14 pKa = 6.35GIASTEE20 pKa = 3.95ANLVAEE26 pKa = 5.01GDD28 pKa = 3.92LEE30 pKa = 4.94AEE32 pKa = 4.45GVLGAEE38 pKa = 4.32GVLGAEE44 pKa = 4.6GDD46 pKa = 4.03LEE48 pKa = 4.57TEE50 pKa = 4.23GDD52 pKa = 4.01LVTEE56 pKa = 4.9GDD58 pKa = 3.99LEE60 pKa = 4.57TEE62 pKa = 4.74GFRR65 pKa = 11.84DD66 pKa = 3.43ADD68 pKa = 3.83FNIASVLQLFFFSCLFFPAIFKK90 pKa = 10.97SFFSGAFCFVFTFLMVSFCFRR111 pKa = 11.84FSFFFSFLGTAKK123 pKa = 10.47LFSPASAEE131 pKa = 3.77ATAVSKK137 pKa = 9.93PWQPCRR143 pKa = 11.84EE144 pKa = 4.04DD145 pKa = 3.07SGLKK149 pKa = 10.14FRR151 pKa = 11.84GEE153 pKa = 3.99QATLAFVTDD162 pKa = 4.05DD163 pKa = 4.04TNGLPCMTFIHH174 pKa = 6.89CLMTPDD180 pKa = 4.41GDD182 pKa = 3.79MPLNSNARR190 pKa = 11.84AHH192 pKa = 5.76SS193 pKa = 3.62

Molecular weight: 20.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A384ZVN4|A0A384ZVN4_9VIRU Uncharacterized protein OS=Bufonid herpesvirus 1 OX=2282206 PE=4 SV=1
MM1 pKa = 6.81VVVAVSHH8 pKa = 6.83LRR10 pKa = 11.84MHH12 pKa = 6.1TNKK15 pKa = 10.09LSHH18 pKa = 6.69HH19 pKa = 6.69FKK21 pKa = 10.64QINRR25 pKa = 11.84CEE27 pKa = 3.84RR28 pKa = 11.84VFFIYY33 pKa = 10.09HH34 pKa = 5.78IVSVHH39 pKa = 4.5VAHH42 pKa = 7.03LASYY46 pKa = 7.71PFHH49 pKa = 6.16YY50 pKa = 10.41VGVVVKK56 pKa = 10.37HH57 pKa = 6.01EE58 pKa = 4.09KK59 pKa = 9.88VQRR62 pKa = 11.84QPLVYY67 pKa = 9.99HH68 pKa = 6.58CRR70 pKa = 11.84QFEE73 pKa = 4.32KK74 pKa = 11.0VLSCLLQNLTAEE86 pKa = 4.4QITFQARR93 pKa = 11.84GAHH96 pKa = 6.42VIQRR100 pKa = 11.84DD101 pKa = 4.06NMCHH105 pKa = 6.23KK106 pKa = 10.08SKK108 pKa = 10.7HH109 pKa = 5.38IFLTHH114 pKa = 5.27KK115 pKa = 10.29HH116 pKa = 4.57VVQTRR121 pKa = 11.84GGQKK125 pKa = 8.66LTRR128 pKa = 11.84CSFFTEE134 pKa = 4.15VRR136 pKa = 11.84RR137 pKa = 11.84HH138 pKa = 4.82GFVVSFHH145 pKa = 5.18SCKK148 pKa = 10.3YY149 pKa = 7.88RR150 pKa = 11.84FCYY153 pKa = 9.34VAHH156 pKa = 6.88LFHH159 pKa = 7.06RR160 pKa = 11.84VVVVNKK166 pKa = 10.63SLVV169 pKa = 3.16

Molecular weight: 19.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

152

0

152

51829

89

2111

341.0

38.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.61 ± 0.137

2.989 ± 0.133

4.631 ± 0.124

5.46 ± 0.242

4.783 ± 0.145

4.399 ± 0.141

2.308 ± 0.104

5.271 ± 0.106

6.645 ± 0.222

9.83 ± 0.176

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.342 ± 0.094

5.402 ± 0.172

4.636 ± 0.163

3.996 ± 0.133

4.443 ± 0.118

7.747 ± 0.191

7.278 ± 0.268

6.697 ± 0.142

1.017 ± 0.067

3.515 ± 0.103
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski