Staphylococcus phage SAP40

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Dubowvirus; unclassified Dubowvirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A513ZXW1|A0A513ZXW1_9CAUD Uncharacterized protein OS=Staphylococcus phage SAP40 OX=2588091 GN=SAP40_00028 PE=4 SV=1
MM1 pKa = 7.51NNRR4 pKa = 11.84EE5 pKa = 4.1QIEE8 pKa = 3.98QSVISASAYY17 pKa = 10.04NGNDD21 pKa = 3.29TEE23 pKa = 4.52GLLKK27 pKa = 10.49EE28 pKa = 4.44IEE30 pKa = 4.24DD31 pKa = 4.28VYY33 pKa = 11.57KK34 pKa = 10.64KK35 pKa = 10.72AQAFDD40 pKa = 4.66EE41 pKa = 4.34ILEE44 pKa = 4.2GLPNAMQDD52 pKa = 3.36ALKK55 pKa = 10.66EE56 pKa = 4.16DD57 pKa = 3.68IYY59 pKa = 11.25LDD61 pKa = 3.66EE62 pKa = 5.14AVGIMTSQVVYY73 pKa = 10.27KK74 pKa = 10.8YY75 pKa = 10.77EE76 pKa = 3.89EE77 pKa = 4.06EE78 pKa = 4.28QEE80 pKa = 3.93NDD82 pKa = 2.92

Molecular weight: 9.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A513ZXW0|A0A513ZXW0_9CAUD Uncharacterized protein OS=Staphylococcus phage SAP40 OX=2588091 GN=SAP40_00031 PE=4 SV=1
MM1 pKa = 7.29ARR3 pKa = 11.84KK4 pKa = 9.59ARR6 pKa = 11.84IVTINDD12 pKa = 2.81KK13 pKa = 10.44PYY15 pKa = 10.06RR16 pKa = 11.84FSKK19 pKa = 11.02FEE21 pKa = 3.76MEE23 pKa = 5.0LIEE26 pKa = 4.34SHH28 pKa = 6.88GITAGMVSKK37 pKa = 10.43RR38 pKa = 11.84VKK40 pKa = 10.67DD41 pKa = 3.3GWEE44 pKa = 3.56LHH46 pKa = 5.97EE47 pKa = 6.15AMDD50 pKa = 4.24APEE53 pKa = 4.22GTRR56 pKa = 11.84LSEE59 pKa = 3.86YY60 pKa = 10.23RR61 pKa = 11.84EE62 pKa = 3.95KK63 pKa = 10.39KK64 pKa = 8.51TIEE67 pKa = 3.99RR68 pKa = 11.84LEE70 pKa = 3.92QARR73 pKa = 11.84LEE75 pKa = 4.26RR76 pKa = 11.84KK77 pKa = 9.75LEE79 pKa = 4.03RR80 pKa = 11.84KK81 pKa = 9.41RR82 pKa = 11.84KK83 pKa = 9.67RR84 pKa = 11.84EE85 pKa = 3.71AEE87 pKa = 3.97LRR89 pKa = 11.84RR90 pKa = 11.84KK91 pKa = 9.76KK92 pKa = 10.2PHH94 pKa = 6.56LFNVPQKK101 pKa = 10.45HH102 pKa = 4.8PRR104 pKa = 11.84GRR106 pKa = 11.84YY107 pKa = 8.83ACYY110 pKa = 10.52LMEE113 pKa = 5.36NDD115 pKa = 4.09IFVKK119 pKa = 10.41VKK121 pKa = 10.3KK122 pKa = 10.51

Molecular weight: 14.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13255

30

1154

210.4

24.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.786 ± 0.438

0.392 ± 0.076

6.262 ± 0.382

7.756 ± 0.604

4.315 ± 0.234

5.326 ± 0.289

1.509 ± 0.157

7.454 ± 0.35

9.325 ± 0.39

7.733 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.444 ± 0.188

7.084 ± 0.316

2.641 ± 0.213

4.097 ± 0.247

4.104 ± 0.24

5.734 ± 0.251

6.367 ± 0.313

6.217 ± 0.35

1.207 ± 0.18

4.247 ± 0.374
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski