Deinococcus sp. HMF7620
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4347 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7C9HU66|A0A7C9HU66_9DEIO Phospholipase OS=Deinococcus sp. HMF7620 OX=2682977 GN=GO986_20555 PE=4 SV=1
MM1 pKa = 7.27 SRR3 pKa = 11.84 LSVRR7 pKa = 11.84 LLCTVVGLSLLSSALAVGTPVNTSISNTATLNYY40 pKa = 9.69 RR41 pKa = 11.84 DD42 pKa = 4.47 ALNAPRR48 pKa = 11.84 TQTSNPVTVTVRR60 pKa = 11.84 QVYY63 pKa = 10.07 ALSLTPDD70 pKa = 3.46 AAEE73 pKa = 3.88 NAIPLSRR80 pKa = 11.84 QFSAQAGASRR90 pKa = 11.84 LIGYY94 pKa = 7.4 EE95 pKa = 3.93 LVNAGNGPDD104 pKa = 3.71 TLTLGVTQSATDD116 pKa = 3.73 GFDD119 pKa = 2.85 VTTRR123 pKa = 11.84 LLPDD127 pKa = 4.29 ANCDD131 pKa = 3.46 GTVDD135 pKa = 3.66 SAVPLGAAQTLAADD149 pKa = 3.95 EE150 pKa = 4.36 RR151 pKa = 11.84 LCVYY155 pKa = 10.53 VEE157 pKa = 3.97 ATVPAGAPTGAAARR171 pKa = 11.84 LTLTAASQGNPAVTDD186 pKa = 3.68 TEE188 pKa = 4.79 NYY190 pKa = 9.9 AQVTVSAAGQLDD202 pKa = 3.38 ITKK205 pKa = 8.65 TVSPTGSALPGAPLTYY221 pKa = 9.1 TVSGSVPAGNPVGAVSGVLTVDD243 pKa = 3.93 GVPRR247 pKa = 11.84 SGVLVRR253 pKa = 11.84 DD254 pKa = 3.59 VLTTLEE260 pKa = 4.37 FGAVTAASASSGAATALYY278 pKa = 8.97 STDD281 pKa = 5.22 GGATWTAAVPASGVNAVALLVEE303 pKa = 4.53 GTGAFLPAGSTLTLSFTASIPAGTLAGTVVRR334 pKa = 11.84 NSAAATLDD342 pKa = 3.58 GNGDD346 pKa = 3.62 GDD348 pKa = 3.92 GTDD351 pKa = 3.41 PGEE354 pKa = 4.41 TPVTPDD360 pKa = 2.61 TTTTVGTVVGAAVGPAAFPQGGASGSYY387 pKa = 7.27 TLRR390 pKa = 11.84 GTAIDD395 pKa = 3.95 RR396 pKa = 11.84 SGDD399 pKa = 3.59 TQTTTTPVTAGGNVTFRR416 pKa = 11.84 QTVLNTGNASNDD428 pKa = 2.94 FTLAVAGAPSGWTCTVQSISSSDD451 pKa = 3.52 TLGTLTNPVTLSAGQSLDD469 pKa = 3.56 FAVDD473 pKa = 3.27 CALPYY478 pKa = 10.39 GAAGTTNVVLTVSATPAGGSADD500 pKa = 3.66 TTTSTVAQVSAAGAPLLGNGDD521 pKa = 4.83 LNPATAPSQAGVTVATNPGTTASFPLEE548 pKa = 4.07 LQNNGPVAEE557 pKa = 4.61 TYY559 pKa = 9.57 TLSSTLTGTQLYY571 pKa = 10.86 ADD573 pKa = 4.47 TNCDD577 pKa = 3.31 GTPDD581 pKa = 3.49 GAAITLTPSVAPGATLCLVAQQPVAAGTAAGEE613 pKa = 4.43 TPVTFTATSTVTPSRR628 pKa = 11.84 TTSVTDD634 pKa = 3.3 TLRR637 pKa = 11.84 VNAVVSGTFGPDD649 pKa = 3.14 GAQSTIPGGSVTYY662 pKa = 10.85 SHH664 pKa = 7.07 TLTNTSNGAADD675 pKa = 3.87 FTVLPFTSAQGFVYY689 pKa = 10.22 SYY691 pKa = 9.17 ATSSGGPFASTLSGTLAPGASTPLFVRR718 pKa = 11.84 VNVPASTAGTPAEE731 pKa = 3.95 AAAISVTLEE740 pKa = 3.84 AQAAPQPAATLQVTDD755 pKa = 3.51 TTTVQNVLASVVKK768 pKa = 9.93 SVRR771 pKa = 11.84 LCADD775 pKa = 3.68 VSCTVTSAITGGQVSPGDD793 pKa = 3.54 VLQYY797 pKa = 10.8 TLTVQNSGSSPLNGAVLIDD816 pKa = 3.7 TVPANTTFRR825 pKa = 11.84 ALGGNNAALLYY836 pKa = 10.49 SVDD839 pKa = 4.91 GGGSWTATPPGALPTGDD856 pKa = 3.76 FQAGLDD862 pKa = 3.84 TNGDD866 pKa = 3.86 NVVDD870 pKa = 4.06 TADD873 pKa = 3.14 ILAPGTSFEE882 pKa = 4.37 VTFTVQVNN890 pKa = 3.38
Molecular weight: 87.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.808
IPC_protein 3.859
Toseland 3.617
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.516
Solomon 3.859
Lehninger 3.808
Nozaki 3.961
DTASelect 4.266
Thurlkill 3.668
EMBOSS 3.821
Sillero 3.973
Patrickios 1.367
IPC_peptide 3.846
IPC2_peptide 3.948
IPC2.peptide.svr19 3.868
Protein with the highest isoelectric point:
>tr|A0A7C9HRY8|A0A7C9HRY8_9DEIO GIY-YIG nuclease family protein OS=Deinococcus sp. HMF7620 OX=2682977 GN=GO986_11375 PE=4 SV=1
MM1 pKa = 6.93 NQRR4 pKa = 11.84 NGALLAVLAMASVAAASPLTGGAQTLVMRR33 pKa = 11.84 LARR36 pKa = 11.84 AGDD39 pKa = 3.5 VSMTGVRR46 pKa = 11.84 WTEE49 pKa = 3.88 DD50 pKa = 2.75 GTTKK54 pKa = 10.35 VVSRR58 pKa = 11.84 AFNAQKK64 pKa = 10.9 YY65 pKa = 9.52 CLTEE69 pKa = 3.59 PTLNLLQRR77 pKa = 11.84 QPTNVGVQAMVFLEE91 pKa = 4.39 FRR93 pKa = 11.84 CVNRR97 pKa = 11.84 IQGKK101 pKa = 9.67 ALTPEE106 pKa = 4.0 LALAFLRR113 pKa = 11.84 SVGYY117 pKa = 8.02 TVSGSNLLTNLLNNPPAANALATASQTAVTPMTPRR152 pKa = 11.84 NN153 pKa = 3.49
Molecular weight: 16.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.604
IPC_protein 10.54
Toseland 10.613
ProMoST 10.379
Dawson 10.73
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.833
Grimsley 10.789
Solomon 10.877
Lehninger 10.833
Nozaki 10.628
DTASelect 10.467
Thurlkill 10.628
EMBOSS 11.038
Sillero 10.672
Patrickios 10.643
IPC_peptide 10.877
IPC2_peptide 9.75
IPC2.peptide.svr19 8.468
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4347
0
4347
1325002
25
3293
304.8
32.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.047 ± 0.065
0.614 ± 0.011
5.051 ± 0.027
5.26 ± 0.036
2.976 ± 0.022
8.971 ± 0.045
2.195 ± 0.024
3.103 ± 0.033
2.354 ± 0.036
11.992 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.733 ± 0.018
2.343 ± 0.028
5.976 ± 0.042
4.326 ± 0.028
7.255 ± 0.037
4.967 ± 0.03
6.362 ± 0.035
7.708 ± 0.032
1.474 ± 0.019
2.293 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here