Aeromonas phage PS
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6H0X6P2|A0A6H0X6P2_9CAUD DNA maturase B OS=Aeromonas phage PS OX=2723762 PE=4 SV=1
MM1 pKa = 7.93 DD2 pKa = 5.18 FAPTTNYY9 pKa = 9.83 IVDD12 pKa = 3.45 VTTADD17 pKa = 3.52 KK18 pKa = 11.37 AEE20 pKa = 4.28 LMAALAGVSGIVSVQDD36 pKa = 3.01 GGVYY40 pKa = 10.09 RR41 pKa = 11.84 EE42 pKa = 4.44 DD43 pKa = 3.25 NAYY46 pKa = 10.21 SQVHH50 pKa = 5.86 IEE52 pKa = 4.22 ALNAVWTLKK61 pKa = 10.64 QLDD64 pKa = 3.78 ALLYY68 pKa = 9.3 SLNYY72 pKa = 10.15 DD73 pKa = 3.3 VVGIVV78 pKa = 3.18
Molecular weight: 8.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.961
IPC_protein 3.859
Toseland 3.643
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.846
Rodwell 3.694
Grimsley 3.567
Solomon 3.834
Lehninger 3.795
Nozaki 3.999
DTASelect 4.24
Thurlkill 3.732
EMBOSS 3.846
Sillero 3.986
Patrickios 0.693
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.862
Protein with the highest isoelectric point:
>tr|A0A6H0X6S9|A0A6H0X6S9_9CAUD DNA primase OS=Aeromonas phage PS OX=2723762 PE=4 SV=1
MM1 pKa = 6.97 RR2 pKa = 11.84 TRR4 pKa = 11.84 RR5 pKa = 11.84 EE6 pKa = 3.87 ATQLRR11 pKa = 11.84 IQRR14 pKa = 11.84 RR15 pKa = 11.84 KK16 pKa = 8.4 EE17 pKa = 3.73 AEE19 pKa = 3.81 EE20 pKa = 4.04 ALEE23 pKa = 3.99 QQRR26 pKa = 11.84 RR27 pKa = 11.84 DD28 pKa = 3.25 AAQKK32 pKa = 10.71 LGISYY37 pKa = 9.31 EE38 pKa = 4.2 SYY40 pKa = 8.48 MARR43 pKa = 11.84 FIHH46 pKa = 6.66 LKK48 pKa = 10.07 VGYY51 pKa = 9.94
Molecular weight: 6.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.296
IPC2_protein 9.633
IPC_protein 10.423
Toseland 10.335
ProMoST 10.145
Dawson 10.526
Bjellqvist 10.248
Wikipedia 10.745
Rodwell 10.687
Grimsley 10.599
Solomon 10.613
Lehninger 10.57
Nozaki 10.292
DTASelect 10.248
Thurlkill 10.379
EMBOSS 10.745
Sillero 10.438
Patrickios 10.452
IPC_peptide 10.599
IPC2_peptide 9.077
IPC2.peptide.svr19 8.625
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
49
0
49
12012
42
991
245.1
27.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.807 ± 0.572
1.199 ± 0.19
5.794 ± 0.192
6.011 ± 0.387
3.272 ± 0.186
7.243 ± 0.396
1.79 ± 0.165
4.62 ± 0.242
5.47 ± 0.326
8.625 ± 0.284
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.697 ± 0.198
4.113 ± 0.293
4.104 ± 0.321
4.462 ± 0.277
5.07 ± 0.29
6.419 ± 0.38
6.227 ± 0.313
7.801 ± 0.341
1.399 ± 0.11
3.879 ± 0.254
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here