Weissella cibaria
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2282 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D1JFW6|A0A0D1JFW6_9LACO WxL domain-containing protein OS=Weissella cibaria OX=137591 GN=QX99_01348 PE=4 SV=1
MM1 pKa = 7.09 TGIVAKK7 pKa = 10.62 DD8 pKa = 3.25 ADD10 pKa = 3.63 ATITVVYY17 pKa = 10.04 NQMGQWVPSFPAGTTDD33 pKa = 3.58 VPSDD37 pKa = 3.2 PMTYY41 pKa = 10.4 PNDD44 pKa = 3.61 PTDD47 pKa = 3.63 PTEE50 pKa = 4.21 PLAPGPEE57 pKa = 4.09 NEE59 pKa = 4.9 NDD61 pKa = 3.76 PGDD64 pKa = 4.11 PANPTLPYY72 pKa = 10.56 VPGFTPQGPDD82 pKa = 3.26 GPLQPVDD89 pKa = 3.72 PANPSSGYY97 pKa = 9.26 WPPTLPSDD105 pKa = 3.59 PTTDD109 pKa = 3.1 TTITYY114 pKa = 8.05 TADD117 pKa = 3.36 EE118 pKa = 4.2 QVATITYY125 pKa = 9.43 IDD127 pKa = 3.71 AVTNEE132 pKa = 4.11 TLGVDD137 pKa = 4.27 RR138 pKa = 11.84 IKK140 pKa = 11.16 GGSDD144 pKa = 2.98 EE145 pKa = 4.24 SHH147 pKa = 7.25 AYY149 pKa = 5.76 TTADD153 pKa = 3.27 RR154 pKa = 11.84 LAAYY158 pKa = 9.27 LVSGYY163 pKa = 10.29 EE164 pKa = 4.17 LVSDD168 pKa = 5.05 GYY170 pKa = 8.65 PTNFTFDD177 pKa = 3.41 RR178 pKa = 11.84 DD179 pKa = 4.05 TTVTQAYY186 pKa = 6.87 TVTLTHH192 pKa = 7.21 GSQTINPNDD201 pKa = 4.04 PKK203 pKa = 11.15 TPGTPINPDD212 pKa = 3.25 NPDD215 pKa = 3.21 GPKK218 pKa = 10.7 YY219 pKa = 10.16 PDD221 pKa = 3.57 GLQATDD227 pKa = 3.98 LNEE230 pKa = 4.23 SVTRR234 pKa = 11.84 TITYY238 pKa = 9.93 VDD240 pKa = 3.43 QAGKK244 pKa = 8.91 TMATAVKK251 pKa = 8.64 EE252 pKa = 4.48 TIHH255 pKa = 5.58 FTRR258 pKa = 11.84 TATLDD263 pKa = 3.88 TVTGAYY269 pKa = 8.02 TYY271 pKa = 10.42 SAWEE275 pKa = 4.12 PTTVTSMDD283 pKa = 3.55 AVDD286 pKa = 4.03 SPVIAGYY293 pKa = 10.12 VADD296 pKa = 4.67 RR297 pKa = 11.84 QTVAALSDD305 pKa = 3.78 LTATSADD312 pKa = 3.29 MSEE315 pKa = 4.1 KK316 pKa = 10.47 VVYY319 pKa = 10.46 SPIGQYY325 pKa = 10.49 VPAFPSGVTDD335 pKa = 3.87 VPSTPIDD342 pKa = 3.76 YY343 pKa = 9.57 PNNPDD348 pKa = 5.3 DD349 pKa = 4.33 PTTTDD354 pKa = 3.55 TPGEE358 pKa = 4.36 DD359 pKa = 3.45 NPATSGTPDD368 pKa = 4.19 NPTMPYY374 pKa = 10.3 VPGFTPQGPDD384 pKa = 3.26 GPLQPVDD391 pKa = 3.51 PRR393 pKa = 11.84 QPKK396 pKa = 9.88 CRR398 pKa = 11.84 LLATSAKK405 pKa = 9.9 RR406 pKa = 11.84 DD407 pKa = 3.65 ANN409 pKa = 3.65
Molecular weight: 43.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.679
IPC2_protein 3.732
IPC_protein 3.783
Toseland 3.541
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.757
Rodwell 3.605
Grimsley 3.439
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.202
Thurlkill 3.605
EMBOSS 3.757
Sillero 3.91
Patrickios 1.036
IPC_peptide 3.783
IPC2_peptide 3.872
IPC2.peptide.svr19 3.792
Protein with the highest isoelectric point:
>tr|A0A0D1M103|A0A0D1M103_9LACO Multidrug resistance protein SMR OS=Weissella cibaria OX=137591 GN=ykkC_2 PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.34 KK9 pKa = 7.47 RR10 pKa = 11.84 HH11 pKa = 5.71 RR12 pKa = 11.84 EE13 pKa = 3.7 RR14 pKa = 11.84 VHH16 pKa = 6.23 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.39 GRR39 pKa = 11.84 KK40 pKa = 8.52 VLSAA44 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 10.862
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.193
Grimsley 12.486
Solomon 12.925
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.93
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2282
0
2282
699463
29
5706
306.5
33.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.204 ± 0.126
0.124 ± 0.007
6.152 ± 0.071
5.314 ± 0.07
4.067 ± 0.057
6.96 ± 0.061
1.904 ± 0.031
6.116 ± 0.06
5.009 ± 0.059
9.416 ± 0.083
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.959 ± 0.039
4.334 ± 0.039
3.657 ± 0.038
4.532 ± 0.047
4.205 ± 0.064
5.422 ± 0.063
7.115 ± 0.106
8.04 ± 0.044
1.172 ± 0.024
3.297 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here