Weissella cibaria

Taxonomy: cellular organisms; Bacteria; Terrabacteria group;

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2282 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0D1JFW6|A0A0D1JFW6_9LACO WxL domain-containing protein OS=Weissella cibaria OX=137591 GN=QX99_01348 PE=4 SV=1
MM1 pKa = 7.09TGIVAKK7 pKa = 10.62DD8 pKa = 3.25ADD10 pKa = 3.63ATITVVYY17 pKa = 10.04NQMGQWVPSFPAGTTDD33 pKa = 3.58VPSDD37 pKa = 3.2PMTYY41 pKa = 10.4PNDD44 pKa = 3.61PTDD47 pKa = 3.63PTEE50 pKa = 4.21PLAPGPEE57 pKa = 4.09NEE59 pKa = 4.9NDD61 pKa = 3.76PGDD64 pKa = 4.11PANPTLPYY72 pKa = 10.56VPGFTPQGPDD82 pKa = 3.26GPLQPVDD89 pKa = 3.72PANPSSGYY97 pKa = 9.26WPPTLPSDD105 pKa = 3.59PTTDD109 pKa = 3.1TTITYY114 pKa = 8.05TADD117 pKa = 3.36EE118 pKa = 4.2QVATITYY125 pKa = 9.43IDD127 pKa = 3.71AVTNEE132 pKa = 4.11TLGVDD137 pKa = 4.27RR138 pKa = 11.84IKK140 pKa = 11.16GGSDD144 pKa = 2.98EE145 pKa = 4.24SHH147 pKa = 7.25AYY149 pKa = 5.76TTADD153 pKa = 3.27RR154 pKa = 11.84LAAYY158 pKa = 9.27LVSGYY163 pKa = 10.29EE164 pKa = 4.17LVSDD168 pKa = 5.05GYY170 pKa = 8.65PTNFTFDD177 pKa = 3.41RR178 pKa = 11.84DD179 pKa = 4.05TTVTQAYY186 pKa = 6.87TVTLTHH192 pKa = 7.21GSQTINPNDD201 pKa = 4.04PKK203 pKa = 11.15TPGTPINPDD212 pKa = 3.25NPDD215 pKa = 3.21GPKK218 pKa = 10.7YY219 pKa = 10.16PDD221 pKa = 3.57GLQATDD227 pKa = 3.98LNEE230 pKa = 4.23SVTRR234 pKa = 11.84TITYY238 pKa = 9.93VDD240 pKa = 3.43QAGKK244 pKa = 8.91TMATAVKK251 pKa = 8.64EE252 pKa = 4.48TIHH255 pKa = 5.58FTRR258 pKa = 11.84TATLDD263 pKa = 3.88TVTGAYY269 pKa = 8.02TYY271 pKa = 10.42SAWEE275 pKa = 4.12PTTVTSMDD283 pKa = 3.55AVDD286 pKa = 4.03SPVIAGYY293 pKa = 10.12VADD296 pKa = 4.67RR297 pKa = 11.84QTVAALSDD305 pKa = 3.78LTATSADD312 pKa = 3.29MSEE315 pKa = 4.1KK316 pKa = 10.47VVYY319 pKa = 10.46SPIGQYY325 pKa = 10.49VPAFPSGVTDD335 pKa = 3.87VPSTPIDD342 pKa = 3.76YY343 pKa = 9.57PNNPDD348 pKa = 5.3DD349 pKa = 4.33PTTTDD354 pKa = 3.55TPGEE358 pKa = 4.36DD359 pKa = 3.45NPATSGTPDD368 pKa = 4.19NPTMPYY374 pKa = 10.3VPGFTPQGPDD384 pKa = 3.26GPLQPVDD391 pKa = 3.51PRR393 pKa = 11.84QPKK396 pKa = 9.88CRR398 pKa = 11.84LLATSAKK405 pKa = 9.9RR406 pKa = 11.84DD407 pKa = 3.65ANN409 pKa = 3.65

Molecular weight:
43.3 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0D1M103|A0A0D1M103_9LACO Multidrug resistance protein SMR OS=Weissella cibaria OX=137591 GN=ykkC_2 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.34KK9 pKa = 7.47RR10 pKa = 11.84HH11 pKa = 5.71RR12 pKa = 11.84EE13 pKa = 3.7RR14 pKa = 11.84VHH16 pKa = 6.23GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.01VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight:
5.38 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2282

0

2282

699463

29

5706

306.5

33.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.204 ± 0.126

0.124 ± 0.007

6.152 ± 0.071

5.314 ± 0.07

4.067 ± 0.057

6.96 ± 0.061

1.904 ± 0.031

6.116 ± 0.06

5.009 ± 0.059

9.416 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.959 ± 0.039

4.334 ± 0.039

3.657 ± 0.038

4.532 ± 0.047

4.205 ± 0.064

5.422 ± 0.063

7.115 ± 0.106

8.04 ± 0.044

1.172 ± 0.024

3.297 ± 0.037

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski