Xenopus laevis endogenous retrovirus Xen1
Average proteome isoelectric point is 4.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8AEW2|Q8AEW2_9RETR Uncharacterized protein OS=Xenopus laevis endogenous retrovirus Xen1 OX=204873 PE=4 SV=1
MM1 pKa = 7.38 SCSNCVCGLSFGITPLGWRR20 pKa = 11.84 LAKK23 pKa = 9.08 ITPGGYY29 pKa = 8.41 PASCLGLGPVLPGFTDD45 pKa = 3.83 FLVYY49 pKa = 10.39 RR50 pKa = 11.84 LNVHH54 pKa = 6.69 FSANPTDD61 pKa = 5.02 LGDD64 pKa = 3.71 SLIDD68 pKa = 2.99 IRR70 pKa = 11.84 ARR72 pKa = 11.84 VASTGQYY79 pKa = 10.61 PEE81 pKa = 4.01 VCDD84 pKa = 3.21 IHH86 pKa = 6.98 PATGLDD92 pKa = 3.12 EE93 pKa = 5.22 CEE95 pKa = 4.15 AEE97 pKa = 4.26 EE98 pKa = 4.07 IVRR101 pKa = 11.84 VGNLLSYY108 pKa = 10.84 LL109 pKa = 3.83
Molecular weight: 11.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.601
IPC2_protein 4.736
IPC_protein 4.558
Toseland 4.406
ProMoST 4.685
Dawson 4.52
Bjellqvist 4.673
Wikipedia 4.431
Rodwell 4.406
Grimsley 4.317
Solomon 4.52
Lehninger 4.469
Nozaki 4.647
DTASelect 4.825
Thurlkill 4.431
EMBOSS 4.444
Sillero 4.685
Patrickios 0.477
IPC_peptide 4.52
IPC2_peptide 4.673
IPC2.peptide.svr19 4.61
Protein with the highest isoelectric point:
>tr|Q8AEW2|Q8AEW2_9RETR Uncharacterized protein OS=Xenopus laevis endogenous retrovirus Xen1 OX=204873 PE=4 SV=1
MM1 pKa = 7.58 SCPEE5 pKa = 4.33 CVCGLVFIISEE16 pKa = 3.91 GGYY19 pKa = 9.55 RR20 pKa = 11.84 LSSVTSKK27 pKa = 10.86 GYY29 pKa = 9.66 KK30 pKa = 8.76 QEE32 pKa = 3.85 EE33 pKa = 4.71 LGRR36 pKa = 11.84 HH37 pKa = 5.84 PIVPEE42 pKa = 3.79 TGLDD46 pKa = 3.67 SFSSQATYY54 pKa = 10.48 VYY56 pKa = 10.06 RR57 pKa = 11.84 LNVHH61 pKa = 6.4 FPVDD65 pKa = 3.91 LDD67 pKa = 4.05 EE68 pKa = 5.11 GWGHH72 pKa = 6.35 SLFEE76 pKa = 4.14 IRR78 pKa = 11.84 KK79 pKa = 8.7 SAFEE83 pKa = 4.59 HH84 pKa = 6.21 GLSPMVCSQHH94 pKa = 7.09 SDD96 pKa = 2.89 TDD98 pKa = 3.89 YY99 pKa = 11.71 LEE101 pKa = 5.63 SEE103 pKa = 4.37 LLDD106 pKa = 4.46 FIRR109 pKa = 11.84 VRR111 pKa = 11.84 SDD113 pKa = 2.62 KK114 pKa = 11.02 AKK116 pKa = 10.57 QLLHH120 pKa = 6.49 SADD123 pKa = 4.02 EE124 pKa = 4.33 FWEE127 pKa = 4.05 NLLRR131 pKa = 11.84 KK132 pKa = 9.42
Molecular weight: 15.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.106
IPC2_protein 5.194
IPC_protein 5.118
Toseland 5.207
ProMoST 5.321
Dawson 5.181
Bjellqvist 5.283
Wikipedia 5.08
Rodwell 5.118
Grimsley 5.181
Solomon 5.181
Lehninger 5.143
Nozaki 5.347
DTASelect 5.486
Thurlkill 5.258
EMBOSS 5.194
Sillero 5.423
Patrickios 3.694
IPC_peptide 5.194
IPC2_peptide 5.423
IPC2.peptide.svr19 5.404
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
241
109
132
120.5
13.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.564 ± 1.228
4.149 ± 0.896
5.809 ± 0.201
7.054 ± 1.63
4.564 ± 0.591
8.714 ± 1.517
3.32 ± 0.981
4.564 ± 0.621
2.905 ± 1.313
12.033 ± 0.536
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.245 ± 0.216
2.49 ± 0.78
5.394 ± 1.286
2.075 ± 0.765
4.979 ± 0.259
9.959 ± 1.731
4.149 ± 0.896
7.054 ± 0.189
1.245 ± 0.216
3.734 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here