Hermit crab associated circular genome
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1RL19|A0A0K1RL19_9CIRC ATP-dependent helicase Rep OS=Hermit crab associated circular genome OX=1692251 PE=3 SV=1
MM1 pKa = 7.62 ISKK4 pKa = 9.76 RR5 pKa = 11.84 WCYY8 pKa = 9.19 TLNNYY13 pKa = 9.91 SEE15 pKa = 5.22 DD16 pKa = 3.62 DD17 pKa = 4.11 VVTMKK22 pKa = 10.81 AVPTTYY28 pKa = 10.05 HH29 pKa = 5.58 VLGKK33 pKa = 10.3 EE34 pKa = 3.98 VGTNGTPHH42 pKa = 6.16 IQGFLTFKK50 pKa = 10.48 SNKK53 pKa = 9.05 RR54 pKa = 11.84 LSAMKK59 pKa = 10.04 KK60 pKa = 8.86 INARR64 pKa = 11.84 AHH66 pKa = 5.39 WEE68 pKa = 4.21 VAKK71 pKa = 9.57 GTSKK75 pKa = 10.45 QAADD79 pKa = 3.63 YY80 pKa = 10.26 CKK82 pKa = 10.35 KK83 pKa = 10.65 DD84 pKa = 3.39 GNFEE88 pKa = 4.16 EE89 pKa = 5.08 LGSVPSQGKK98 pKa = 7.96 RR99 pKa = 11.84 TDD101 pKa = 3.8 LEE103 pKa = 4.29 DD104 pKa = 3.66 AAVMITAGEE113 pKa = 4.1 SLKK116 pKa = 10.65 RR117 pKa = 11.84 VAEE120 pKa = 4.0 EE121 pKa = 4.16 HH122 pKa = 5.76 PTVVIKK128 pKa = 8.45 YY129 pKa = 9.43 HH130 pKa = 6.6 KK131 pKa = 10.12 GLSALKK137 pKa = 10.35 SLLSEE142 pKa = 4.12 SRR144 pKa = 11.84 EE145 pKa = 3.94 TDD147 pKa = 3.17 DD148 pKa = 5.47 VRR150 pKa = 11.84 GIWLHH155 pKa = 6.11 GPPGCGKK162 pKa = 9.5 SHH164 pKa = 7.9 LARR167 pKa = 11.84 TVSPYY172 pKa = 11.19 LKK174 pKa = 10.41 AQNKK178 pKa = 7.01 WWDD181 pKa = 3.57 GYY183 pKa = 11.74 ANEE186 pKa = 4.77 EE187 pKa = 4.22 YY188 pKa = 11.34 VLIDD192 pKa = 5.01 DD193 pKa = 4.5 FDD195 pKa = 5.11 KK196 pKa = 11.4 GGKK199 pKa = 9.41 CLGHH203 pKa = 5.98 YY204 pKa = 10.0 LKK206 pKa = 10.89 LWADD210 pKa = 3.54 KK211 pKa = 10.82 YY212 pKa = 10.55 EE213 pKa = 4.28 CTGEE217 pKa = 3.98 IKK219 pKa = 10.74 GATVALNHH227 pKa = 6.7 RR228 pKa = 11.84 KK229 pKa = 9.47 FIITSNYY236 pKa = 9.29 HH237 pKa = 5.95 PDD239 pKa = 3.76 EE240 pKa = 5.25 IFDD243 pKa = 4.51 DD244 pKa = 4.92 DD245 pKa = 4.63 SVLLEE250 pKa = 4.64 AITRR254 pKa = 11.84 RR255 pKa = 11.84 FSIRR259 pKa = 11.84 SCWHH263 pKa = 6.3 HH264 pKa = 6.69 SRR266 pKa = 11.84 EE267 pKa = 4.11 ADD269 pKa = 3.6 AVWFDD274 pKa = 3.72 SLTKK278 pKa = 10.36 KK279 pKa = 9.3 EE280 pKa = 3.99 AHH282 pKa = 6.51 AATANKK288 pKa = 10.08
Molecular weight: 32.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.117
IPC2_protein 7.483
IPC_protein 7.424
Toseland 7.263
ProMoST 8.097
Dawson 8.126
Bjellqvist 8.39
Wikipedia 8.053
Rodwell 8.141
Grimsley 7.337
Solomon 8.229
Lehninger 8.244
Nozaki 8.609
DTASelect 8.2
Thurlkill 8.273
EMBOSS 8.361
Sillero 8.565
Patrickios 4.457
IPC_peptide 8.229
IPC2_peptide 7.38
IPC2.peptide.svr19 7.489
Protein with the highest isoelectric point:
>tr|A0A0K1RL19|A0A0K1RL19_9CIRC ATP-dependent helicase Rep OS=Hermit crab associated circular genome OX=1692251 PE=3 SV=1
MM1 pKa = 7.62 ISKK4 pKa = 9.76 RR5 pKa = 11.84 WCYY8 pKa = 9.19 TLNNYY13 pKa = 9.91 SEE15 pKa = 5.22 DD16 pKa = 3.62 DD17 pKa = 4.11 VVTMKK22 pKa = 10.81 AVPTTYY28 pKa = 10.05 HH29 pKa = 5.58 VLGKK33 pKa = 10.3 EE34 pKa = 3.98 VGTNGTPHH42 pKa = 6.16 IQGFLTFKK50 pKa = 10.48 SNKK53 pKa = 9.05 RR54 pKa = 11.84 LSAMKK59 pKa = 10.04 KK60 pKa = 8.86 INARR64 pKa = 11.84 AHH66 pKa = 5.39 WEE68 pKa = 4.21 VAKK71 pKa = 9.57 GTSKK75 pKa = 10.45 QAADD79 pKa = 3.63 YY80 pKa = 10.26 CKK82 pKa = 10.35 KK83 pKa = 10.65 DD84 pKa = 3.39 GNFEE88 pKa = 4.16 EE89 pKa = 5.08 LGSVPSQGKK98 pKa = 7.96 RR99 pKa = 11.84 TDD101 pKa = 3.8 LEE103 pKa = 4.29 DD104 pKa = 3.66 AAVMITAGEE113 pKa = 4.1 SLKK116 pKa = 10.65 RR117 pKa = 11.84 VAEE120 pKa = 4.0 EE121 pKa = 4.16 HH122 pKa = 5.76 PTVVIKK128 pKa = 8.45 YY129 pKa = 9.43 HH130 pKa = 6.6 KK131 pKa = 10.12 GLSALKK137 pKa = 10.35 SLLSEE142 pKa = 4.12 SRR144 pKa = 11.84 EE145 pKa = 3.94 TDD147 pKa = 3.17 DD148 pKa = 5.47 VRR150 pKa = 11.84 GIWLHH155 pKa = 6.11 GPPGCGKK162 pKa = 9.5 SHH164 pKa = 7.9 LARR167 pKa = 11.84 TVSPYY172 pKa = 11.19 LKK174 pKa = 10.41 AQNKK178 pKa = 7.01 WWDD181 pKa = 3.57 GYY183 pKa = 11.74 ANEE186 pKa = 4.77 EE187 pKa = 4.22 YY188 pKa = 11.34 VLIDD192 pKa = 5.01 DD193 pKa = 4.5 FDD195 pKa = 5.11 KK196 pKa = 11.4 GGKK199 pKa = 9.41 CLGHH203 pKa = 5.98 YY204 pKa = 10.0 LKK206 pKa = 10.89 LWADD210 pKa = 3.54 KK211 pKa = 10.82 YY212 pKa = 10.55 EE213 pKa = 4.28 CTGEE217 pKa = 3.98 IKK219 pKa = 10.74 GATVALNHH227 pKa = 6.7 RR228 pKa = 11.84 KK229 pKa = 9.47 FIITSNYY236 pKa = 9.29 HH237 pKa = 5.95 PDD239 pKa = 3.76 EE240 pKa = 5.25 IFDD243 pKa = 4.51 DD244 pKa = 4.92 DD245 pKa = 4.63 SVLLEE250 pKa = 4.64 AITRR254 pKa = 11.84 RR255 pKa = 11.84 FSIRR259 pKa = 11.84 SCWHH263 pKa = 6.3 HH264 pKa = 6.69 SRR266 pKa = 11.84 EE267 pKa = 4.11 ADD269 pKa = 3.6 AVWFDD274 pKa = 3.72 SLTKK278 pKa = 10.36 KK279 pKa = 9.3 EE280 pKa = 3.99 AHH282 pKa = 6.51 AATANKK288 pKa = 10.08
Molecular weight: 32.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.117
IPC2_protein 7.483
IPC_protein 7.424
Toseland 7.263
ProMoST 8.097
Dawson 8.126
Bjellqvist 8.39
Wikipedia 8.053
Rodwell 8.141
Grimsley 7.337
Solomon 8.229
Lehninger 8.244
Nozaki 8.609
DTASelect 8.2
Thurlkill 8.273
EMBOSS 8.361
Sillero 8.565
Patrickios 4.457
IPC_peptide 8.229
IPC2_peptide 7.38
IPC2.peptide.svr19 7.489
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
288
288
288
288.0
32.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.681 ± 0.0
2.083 ± 0.0
6.597 ± 0.0
6.597 ± 0.0
2.778 ± 0.0
6.944 ± 0.0
4.514 ± 0.0
4.514 ± 0.0
9.722 ± 0.0
7.639 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.389 ± 0.0
3.819 ± 0.0
2.778 ± 0.0
1.389 ± 0.0
4.514 ± 0.0
6.944 ± 0.0
6.597 ± 0.0
5.903 ± 0.0
2.778 ± 0.0
3.819 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here