Mungbean yellow mosaic India virus
Average proteome isoelectric point is 8.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9WH22|Q9WH22_9GEMI Replication enhancer OS=Mungbean yellow mosaic India virus OX=223287 GN=AC3 PE=3 SV=1
MM1 pKa = 7.9 VLILRR6 pKa = 11.84 CLLVVVNNIIINLEE20 pKa = 3.94 EE21 pKa = 4.52 TIHH24 pKa = 6.49 HH25 pKa = 6.32 GLFFTSILATSNSGTKK41 pKa = 10.6 LSQDD45 pKa = 5.05 LITITQIVLHH55 pKa = 6.29 SGCTGLVVIHH65 pKa = 6.08 VEE67 pKa = 4.18 HH68 pKa = 6.44 LTKK71 pKa = 10.39 IHH73 pKa = 6.9 GCTKK77 pKa = 9.34 WSSVTTT83 pKa = 4.04
Molecular weight: 9.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.601
IPC2_protein 6.44
IPC_protein 6.766
Toseland 6.825
ProMoST 7.0
Dawson 7.234
Bjellqvist 7.0
Wikipedia 7.205
Rodwell 7.205
Grimsley 6.942
Solomon 7.307
Lehninger 7.322
Nozaki 7.38
DTASelect 7.512
Thurlkill 7.541
EMBOSS 7.614
Sillero 7.629
Patrickios 4.024
IPC_peptide 7.322
IPC2_peptide 7.366
IPC2.peptide.svr19 7.21
Protein with the highest isoelectric point:
>tr|Q9WH23|Q9WH23_9GEMI Transcriptional activator protein OS=Mungbean yellow mosaic India virus OX=223287 GN=AC2 PE=3 SV=1
MM1 pKa = 8.31 DD2 pKa = 4.43 FRR4 pKa = 11.84 TGEE7 pKa = 4.37 NITAAQLRR15 pKa = 11.84 SGVYY19 pKa = 8.64 IWEE22 pKa = 4.28 VQNPLSFKK30 pKa = 10.41 IMHH33 pKa = 6.97 HH34 pKa = 5.82 SPILAEE40 pKa = 4.06 SRR42 pKa = 11.84 MNGHH46 pKa = 5.91 QAAHH50 pKa = 6.08 MFNHH54 pKa = 6.73 RR55 pKa = 11.84 LKK57 pKa = 10.64 KK58 pKa = 10.07 ALGMHH63 pKa = 6.58 KK64 pKa = 10.4 CFLDD68 pKa = 3.52 LKK70 pKa = 9.75 LYY72 pKa = 10.47 HH73 pKa = 6.52 YY74 pKa = 9.28 MKK76 pKa = 9.56 ATSGMILSIFRR87 pKa = 11.84 NQLFKK92 pKa = 10.9 FLNNLGVISLANILYY107 pKa = 8.86 FASDD111 pKa = 3.3 FLYY114 pKa = 11.11 NKK116 pKa = 8.86 TPLGGGCKK124 pKa = 10.2 LYY126 pKa = 11.07 AA127 pKa = 4.49
Molecular weight: 14.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.172
IPC2_protein 9.253
IPC_protein 9.209
Toseland 9.765
ProMoST 9.531
Dawson 10.028
Bjellqvist 9.736
Wikipedia 10.204
Rodwell 10.379
Grimsley 10.101
Solomon 10.058
Lehninger 10.028
Nozaki 9.823
DTASelect 9.706
Thurlkill 9.853
EMBOSS 10.189
Sillero 9.955
Patrickios 7.541
IPC_peptide 10.058
IPC2_peptide 8.478
IPC2.peptide.svr19 8.193
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
1686
74
361
187.3
21.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.219 ± 0.619
2.372 ± 0.284
4.686 ± 0.357
4.567 ± 0.512
4.389 ± 0.408
5.397 ± 0.509
3.321 ± 0.482
6.287 ± 0.806
6.88 ± 0.466
8.66 ± 0.905
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.669 ± 0.515
6.05 ± 0.359
5.279 ± 0.457
3.796 ± 0.321
6.109 ± 0.455
8.363 ± 0.759
5.635 ± 0.664
4.923 ± 0.652
1.246 ± 0.12
4.152 ± 0.397
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here