Shuangao insect virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEF4|A0A1L3KEF4_9VIRU RNA-directed RNA polymerase OS=Shuangao insect virus 9 OX=1923470 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84MLALEE7 pKa = 4.89AGDD10 pKa = 4.06LQADD14 pKa = 4.72HH15 pKa = 6.9IRR17 pKa = 11.84VFVKK21 pKa = 10.23PEE23 pKa = 3.5PHH25 pKa = 6.06KK26 pKa = 10.16QAKK29 pKa = 10.03LEE31 pKa = 3.99EE32 pKa = 4.39GRR34 pKa = 11.84LRR36 pKa = 11.84LISAVSLVDD45 pKa = 3.27TCVDD49 pKa = 4.23RR50 pKa = 11.84ILFTWCLEE58 pKa = 3.93AALDD62 pKa = 4.03NFLTTPSMIGWTPIYY77 pKa = 10.44GSWRR81 pKa = 11.84QLWALYY87 pKa = 10.31GGSALALDD95 pKa = 4.22KK96 pKa = 11.25SAWDD100 pKa = 3.33WTVQPWLIDD109 pKa = 4.27AIRR112 pKa = 11.84DD113 pKa = 3.9VIKK116 pKa = 10.45EE117 pKa = 3.98LAIDD121 pKa = 4.81APPWWQALVDD131 pKa = 4.04RR132 pKa = 11.84RR133 pKa = 11.84FEE135 pKa = 4.56LLFQSPVFEE144 pKa = 4.52FQDD147 pKa = 3.59GTTVKK152 pKa = 10.67QMGKK156 pKa = 10.83GIMKK160 pKa = 9.81SGCYY164 pKa = 8.43LTIIANTLGQVITHH178 pKa = 6.52FVACQQTGDD187 pKa = 4.0NPVLSVPHH195 pKa = 6.76ALGDD199 pKa = 3.74DD200 pKa = 4.44TIQRR204 pKa = 11.84GDD206 pKa = 3.86RR207 pKa = 11.84INDD210 pKa = 2.99IGLYY214 pKa = 9.75CAALEE219 pKa = 4.17QLGCKK224 pKa = 9.78VKK226 pKa = 10.56GGQVATDD233 pKa = 3.32VVEE236 pKa = 4.42FVGFEE241 pKa = 4.02FRR243 pKa = 11.84EE244 pKa = 4.48HH245 pKa = 6.11SCVPAYY251 pKa = 10.21RR252 pKa = 11.84EE253 pKa = 3.59KK254 pKa = 10.87HH255 pKa = 5.45FYY257 pKa = 9.51KK258 pKa = 10.62QEE260 pKa = 3.85FTPDD264 pKa = 3.07LGGFLYY270 pKa = 10.81AMQLLYY276 pKa = 11.14AHH278 pKa = 7.39DD279 pKa = 4.04PAFYY283 pKa = 10.14AYY285 pKa = 9.92YY286 pKa = 10.29RR287 pKa = 11.84RR288 pKa = 11.84LAAEE292 pKa = 4.23KK293 pKa = 9.84VPSRR297 pKa = 11.84VTSAIEE303 pKa = 3.85AKK305 pKa = 10.78GFMAA309 pKa = 6.13

Molecular weight: 34.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEF4|A0A1L3KEF4_9VIRU RNA-directed RNA polymerase OS=Shuangao insect virus 9 OX=1923470 PE=4 SV=1
MM1 pKa = 7.6LWFSSQPDD9 pKa = 3.25KK10 pKa = 10.94MVTVSFEE17 pKa = 3.9IPEE20 pKa = 4.23KK21 pKa = 10.53KK22 pKa = 9.73VSKK25 pKa = 10.4VKK27 pKa = 10.66LVTRR31 pKa = 11.84YY32 pKa = 8.45GWGWYY37 pKa = 9.84RR38 pKa = 11.84FIGLFWPIIVASQLYY53 pKa = 8.85MLAFYY58 pKa = 10.9LSLDD62 pKa = 3.09ATTWQFAFSQTVAEE76 pKa = 4.1PVVRR80 pKa = 11.84LVNAVFSTLVLCFVVYY96 pKa = 10.2LMRR99 pKa = 11.84GTMRR103 pKa = 11.84LTGLCKK109 pKa = 10.61DD110 pKa = 3.61PTGSVTFEE118 pKa = 4.25PEE120 pKa = 3.32RR121 pKa = 11.84MRR123 pKa = 11.84PGSLLMPHH131 pKa = 7.13APSKK135 pKa = 10.68CQAEE139 pKa = 4.81LYY141 pKa = 10.79GKK143 pKa = 7.29TQGTWVRR150 pKa = 11.84LGQAFRR156 pKa = 11.84SSNYY160 pKa = 10.07LVTASHH166 pKa = 7.07VIDD169 pKa = 3.33QVEE172 pKa = 4.17IVKK175 pKa = 8.67ITTEE179 pKa = 3.79HH180 pKa = 6.71ASIEE184 pKa = 4.45VPTSLFRR191 pKa = 11.84QVEE194 pKa = 4.29GDD196 pKa = 3.76LSIMPVTDD204 pKa = 3.53QVLAPLKK211 pKa = 10.12LANGRR216 pKa = 11.84LASHH220 pKa = 7.05EE221 pKa = 4.06ATEE224 pKa = 4.55GSGLFVKK231 pKa = 10.53IGGFGSGSLGMLKK244 pKa = 10.2PNPAFGFVEE253 pKa = 4.55YY254 pKa = 9.97TGSTVPGFSGAPYY267 pKa = 10.14VVGDD271 pKa = 4.1VIYY274 pKa = 10.59GMHH277 pKa = 7.04LGGGAQNLGYY287 pKa = 9.9SGAYY291 pKa = 9.27ISMLMRR297 pKa = 11.84ALQEE301 pKa = 4.44DD302 pKa = 3.88STDD305 pKa = 3.65FLMSQIEE312 pKa = 4.08RR313 pKa = 11.84AAEE316 pKa = 4.01YY317 pKa = 10.15QVSQSPYY324 pKa = 9.85DD325 pKa = 3.76PDD327 pKa = 3.36EE328 pKa = 4.52FRR330 pKa = 11.84VKK332 pKa = 10.78VGGRR336 pKa = 11.84YY337 pKa = 9.43YY338 pKa = 11.12LLDD341 pKa = 3.89AEE343 pKa = 5.31GVHH346 pKa = 6.41KK347 pKa = 11.02LNLRR351 pKa = 11.84VQRR354 pKa = 11.84DD355 pKa = 3.53VSSGYY360 pKa = 10.05RR361 pKa = 11.84DD362 pKa = 3.86YY363 pKa = 11.2EE364 pKa = 4.14PEE366 pKa = 4.08GALPQVEE373 pKa = 4.53KK374 pKa = 10.82VVKK377 pKa = 9.86PVVIPATSPKK387 pKa = 10.03PPMARR392 pKa = 11.84VEE394 pKa = 4.18EE395 pKa = 4.58LEE397 pKa = 4.83SLLQTGNGPAPAEE410 pKa = 4.17NPVPAGATGQDD421 pKa = 3.57SVPARR426 pKa = 11.84PARR429 pKa = 11.84RR430 pKa = 11.84PRR432 pKa = 11.84RR433 pKa = 11.84RR434 pKa = 11.84SPAATTSSATLPSSATDD451 pKa = 3.96GQDD454 pKa = 2.95QTPAQLRR461 pKa = 11.84GALQDD466 pKa = 3.37IADD469 pKa = 4.16SLKK472 pKa = 10.02MLRR475 pKa = 11.84KK476 pKa = 9.48EE477 pKa = 4.1LGTLQMQTSRR487 pKa = 11.84RR488 pKa = 11.84RR489 pKa = 11.84LKK491 pKa = 10.4EE492 pKa = 3.98SEE494 pKa = 4.26SASATGRR501 pKa = 11.84LTSLRR506 pKa = 11.84RR507 pKa = 11.84EE508 pKa = 4.23LSRR511 pKa = 11.84LRR513 pKa = 11.84SSGGSQQ519 pKa = 2.98

Molecular weight: 56.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

828

309

519

414.0

45.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.696 ± 0.789

1.208 ± 0.624

4.71 ± 1.04

5.435 ± 0.152

4.227 ± 0.561

8.092 ± 0.574

1.57 ± 0.41

3.865 ± 0.966

4.106 ± 0.25

10.145 ± 0.125

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.778 ± 0.302

1.449 ± 0.091

5.918 ± 0.818

5.072 ± 0.062

6.159 ± 0.579

7.367 ± 2.055

5.918 ± 0.437

7.971 ± 0.502

1.812 ± 0.65

3.502 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski