Streptococcus phage IPP8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5S7S9|A0A1S5S7S9_9CAUD Uncharacterized protein OS=Streptococcus phage IPP8 OX=1916206 GN=IPP8_00025 PE=4 SV=1
MM1 pKa = 7.28DD2 pKa = 4.2TARR5 pKa = 11.84IGITNVEE12 pKa = 4.08FSGTGEE18 pKa = 4.01NDD20 pKa = 3.15SATVKK25 pKa = 10.92LEE27 pKa = 3.91LNIYY31 pKa = 9.84GADD34 pKa = 3.23TFSAIEE40 pKa = 4.46LLPKK44 pKa = 10.13ILTDD48 pKa = 3.24IHH50 pKa = 6.43SLSYY54 pKa = 10.72EE55 pKa = 3.71VDD57 pKa = 3.08

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5S7Q7|A0A1S5S7Q7_9CAUD Uncharacterized protein OS=Streptococcus phage IPP8 OX=1916206 GN=IPP8_00012 PE=4 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84PRR4 pKa = 11.84RR5 pKa = 11.84YY6 pKa = 9.18PYY8 pKa = 10.05SGKK11 pKa = 10.15KK12 pKa = 9.81EE13 pKa = 4.19STFVKK18 pKa = 10.34ADD20 pKa = 3.45PEE22 pKa = 4.2LVEE25 pKa = 4.92KK26 pKa = 10.39ILRR29 pKa = 11.84NISFLEE35 pKa = 3.81HH36 pKa = 6.02LQVVLATKK44 pKa = 10.33LL45 pKa = 3.41

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12375

45

2147

206.3

23.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.982 ± 0.593

0.493 ± 0.087

6.109 ± 0.329

8.137 ± 0.526

4.024 ± 0.246

6.352 ± 0.609

1.398 ± 0.172

6.489 ± 0.279

8.743 ± 0.381

8.307 ± 0.253

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.594 ± 0.221

5.164 ± 0.295

2.772 ± 0.266

4.065 ± 0.226

4.533 ± 0.316

6.149 ± 0.379

6.319 ± 0.341

6.19 ± 0.256

1.341 ± 0.149

3.838 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski