Pandoravirus neocaledonia
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1081 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U7UB11|A0A2U7UB11_9VIRU Uncharacterized protein OS=Pandoravirus neocaledonia OX=2107708 GN=pneo_cds_34 PE=4 SV=1
MM1 pKa = 7.74 SDD3 pKa = 3.08 PRR5 pKa = 11.84 LGYY8 pKa = 10.06 AYY10 pKa = 10.31 SYY12 pKa = 10.63 GAEE15 pKa = 3.66 PSGFVGAAPPSAAGAVWRR33 pKa = 11.84 GTGAPRR39 pKa = 11.84 TRR41 pKa = 11.84 EE42 pKa = 3.92 LGRR45 pKa = 11.84 SNAAAPLAWNAQPPFGAALAQQQQQQHH72 pKa = 5.78 TLRR75 pKa = 11.84 QPPPAYY81 pKa = 10.16 SGQSYY86 pKa = 10.23 GGAVAAPYY94 pKa = 10.4 ASSGYY99 pKa = 10.56 SDD101 pKa = 5.4 DD102 pKa = 4.24 EE103 pKa = 4.74 GSAGSDD109 pKa = 2.93 ADD111 pKa = 4.26 GAFSDD116 pKa = 5.57 DD117 pKa = 4.32 AASDD121 pKa = 3.97 DD122 pKa = 4.3 DD123 pKa = 5.34 DD124 pKa = 4.35 GVVYY128 pKa = 10.8 SDD130 pKa = 5.58 DD131 pKa = 3.76 EE132 pKa = 5.09 NGDD135 pKa = 3.3 EE136 pKa = 4.43 GARR139 pKa = 11.84 GSWDD143 pKa = 3.11 GGVYY147 pKa = 10.12 GAAPAQSSWWW157 pKa = 3.51
Molecular weight: 15.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.834
IPC_protein 3.821
Toseland 3.592
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.808
Rodwell 3.643
Grimsley 3.503
Solomon 3.821
Lehninger 3.77
Nozaki 3.948
DTASelect 4.228
Thurlkill 3.668
EMBOSS 3.808
Sillero 3.948
Patrickios 0.947
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A2U7UD00|A0A2U7UD00_9VIRU Uncharacterized protein OS=Pandoravirus neocaledonia OX=2107708 GN=pneo_cds_603 PE=4 SV=1
MM1 pKa = 7.48 GANDD5 pKa = 3.38 SRR7 pKa = 11.84 SAVTMHH13 pKa = 5.53 VRR15 pKa = 11.84 AATDD19 pKa = 3.29 RR20 pKa = 11.84 SICGKK25 pKa = 10.03 GCSYY29 pKa = 10.85 CHH31 pKa = 5.95 NKK33 pKa = 10.43 AYY35 pKa = 9.82 IHH37 pKa = 6.06 VVANDD42 pKa = 3.6 GSGRR46 pKa = 11.84 FLLPLRR52 pKa = 11.84 EE53 pKa = 4.15 RR54 pKa = 11.84 LPTVPRR60 pKa = 11.84 VVQRR64 pKa = 11.84 APHH67 pKa = 6.58 KK68 pKa = 10.58 DD69 pKa = 3.16 VSLWQVRR76 pKa = 11.84 RR77 pKa = 11.84 VRR79 pKa = 11.84 HH80 pKa = 6.28 ADD82 pKa = 3.11 TAAATAQKK90 pKa = 10.29 RR91 pKa = 11.84 RR92 pKa = 11.84 RR93 pKa = 11.84 QHH95 pKa = 5.66 HH96 pKa = 6.92 AGPGHH101 pKa = 6.19 CHH103 pKa = 6.12 GAPRR107 pKa = 11.84 WRR109 pKa = 11.84 QVSRR113 pKa = 11.84 DD114 pKa = 3.75 EE115 pKa = 4.12 QPAAVAPWPSSLGFASVLCDD135 pKa = 3.94 GEE137 pKa = 4.55 HH138 pKa = 7.15 KK139 pKa = 10.5 EE140 pKa = 4.14
Molecular weight: 15.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.399
IPC_protein 10.058
Toseland 10.511
ProMoST 10.204
Dawson 10.613
Bjellqvist 10.35
Wikipedia 10.818
Rodwell 10.76
Grimsley 10.657
Solomon 10.745
Lehninger 10.716
Nozaki 10.57
DTASelect 10.321
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.57
Patrickios 10.526
IPC_peptide 10.745
IPC2_peptide 9.736
IPC2.peptide.svr19 8.471
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1081
0
1081
412939
51
2360
382.0
41.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.084 ± 0.094
2.611 ± 0.048
7.394 ± 0.081
3.934 ± 0.044
2.307 ± 0.032
7.899 ± 0.08
3.005 ± 0.039
3.203 ± 0.039
2.208 ± 0.051
7.89 ± 0.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.039 ± 0.026
2.221 ± 0.036
6.307 ± 0.073
2.688 ± 0.043
8.926 ± 0.066
6.168 ± 0.066
5.656 ± 0.06
7.32 ± 0.05
1.966 ± 0.04
2.171 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here