Amino acid dipepetide frequency for Paenibacillus sp. 598K

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.16AlaAla: 11.16 ± 0.112
0.801AlaCys: 0.801 ± 0.02
5.248AlaAsp: 5.248 ± 0.051
6.739AlaGlu: 6.739 ± 0.07
3.341AlaPhe: 3.341 ± 0.035
7.649AlaGly: 7.649 ± 0.07
1.666AlaHis: 1.666 ± 0.029
5.519AlaIle: 5.519 ± 0.047
3.362AlaLys: 3.362 ± 0.039
9.736AlaLeu: 9.736 ± 0.077
2.63AlaMet: 2.63 ± 0.035
2.614AlaAsn: 2.614 ± 0.038
3.462AlaPro: 3.462 ± 0.043
3.408AlaGln: 3.408 ± 0.042
5.016AlaArg: 5.016 ± 0.052
6.093AlaSer: 6.093 ± 0.06
4.797AlaThr: 4.797 ± 0.079
6.869AlaVal: 6.869 ± 0.063
1.303AlaTrp: 1.303 ± 0.024
3.264AlaTyr: 3.264 ± 0.038
0.0AlaXaa: 0.0 ± 0.0
Cys
0.575CysAla: 0.575 ± 0.017
0.106CysCys: 0.106 ± 0.007
0.379CysAsp: 0.379 ± 0.017
0.45CysGlu: 0.45 ± 0.014
0.247CysPhe: 0.247 ± 0.01
0.711CysGly: 0.711 ± 0.021
0.179CysHis: 0.179 ± 0.009
0.401CysIle: 0.401 ± 0.016
0.231CysLys: 0.231 ± 0.01
0.699CysLeu: 0.699 ± 0.017
0.178CysMet: 0.178 ± 0.009
0.228CysAsn: 0.228 ± 0.01
0.323CysPro: 0.323 ± 0.013
0.209CysGln: 0.209 ± 0.01
0.485CysArg: 0.485 ± 0.014
0.513CysSer: 0.513 ± 0.014
0.348CysThr: 0.348 ± 0.012
0.439CysVal: 0.439 ± 0.014
0.096CysTrp: 0.096 ± 0.007
0.275CysTyr: 0.275 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
4.851AspAla: 4.851 ± 0.05
0.364AspCys: 0.364 ± 0.014
2.689AspAsp: 2.689 ± 0.039
4.099AspGlu: 4.099 ± 0.048
1.874AspPhe: 1.874 ± 0.03
4.848AspGly: 4.848 ± 0.056
1.131AspHis: 1.131 ± 0.025
3.147AspIle: 3.147 ± 0.039
2.244AspLys: 2.244 ± 0.033
4.542AspLeu: 4.542 ± 0.04
1.318AspMet: 1.318 ± 0.023
1.852AspAsn: 1.852 ± 0.031
2.668AspPro: 2.668 ± 0.037
1.962AspGln: 1.962 ± 0.029
3.368AspArg: 3.368 ± 0.04
2.663AspSer: 2.663 ± 0.037
2.694AspThr: 2.694 ± 0.038
3.379AspVal: 3.379 ± 0.046
1.027AspTrp: 1.027 ± 0.023
2.411AspTyr: 2.411 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
7.426GluAla: 7.426 ± 0.07
0.307GluCys: 0.307 ± 0.012
3.114GluAsp: 3.114 ± 0.04
5.203GluGlu: 5.203 ± 0.065
1.712GluPhe: 1.712 ± 0.03
4.724GluGly: 4.724 ± 0.059
1.563GluHis: 1.563 ± 0.029
3.56GluIle: 3.56 ± 0.046
2.462GluLys: 2.462 ± 0.041
8.375GluLeu: 8.375 ± 0.073
1.888GluMet: 1.888 ± 0.03
1.542GluAsn: 1.542 ± 0.027
2.896GluPro: 2.896 ± 0.039
4.415GluGln: 4.415 ± 0.053
5.249GluArg: 5.249 ± 0.059
3.463GluSer: 3.463 ± 0.038
3.483GluThr: 3.483 ± 0.045
4.362GluVal: 4.362 ± 0.049
0.944GluTrp: 0.944 ± 0.022
1.782GluTyr: 1.782 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
3.266PheAla: 3.266 ± 0.04
0.275PheCys: 0.275 ± 0.011
2.21PheAsp: 2.21 ± 0.032
2.26PheGlu: 2.26 ± 0.035
1.559PhePhe: 1.559 ± 0.033
2.881PheGly: 2.881 ± 0.036
0.8PheHis: 0.8 ± 0.015
2.238PheIle: 2.238 ± 0.036
1.508PheLys: 1.508 ± 0.03
3.4PheLeu: 3.4 ± 0.044
0.928PheMet: 0.928 ± 0.022
1.4PheAsn: 1.4 ± 0.027
1.398PhePro: 1.398 ± 0.026
1.247PheGln: 1.247 ± 0.024
2.166PheArg: 2.166 ± 0.034
2.327PheSer: 2.327 ± 0.035
2.13PheThr: 2.13 ± 0.034
2.78PheVal: 2.78 ± 0.04
0.493PheTrp: 0.493 ± 0.016
1.392PheTyr: 1.392 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
6.951GlyAla: 6.951 ± 0.091
0.71GlyCys: 0.71 ± 0.018
3.958GlyAsp: 3.958 ± 0.045
5.394GlyGlu: 5.394 ± 0.056
2.92GlyPhe: 2.92 ± 0.041
6.251GlyGly: 6.251 ± 0.084
1.472GlyHis: 1.472 ± 0.026
4.941GlyIle: 4.941 ± 0.052
3.406GlyLys: 3.406 ± 0.046
7.678GlyLeu: 7.678 ± 0.064
2.4GlyMet: 2.4 ± 0.035
2.438GlyAsn: 2.438 ± 0.039
2.292GlyPro: 2.292 ± 0.048
3.038GlyGln: 3.038 ± 0.036
4.267GlyArg: 4.267 ± 0.051
4.909GlySer: 4.909 ± 0.051
4.363GlyThr: 4.363 ± 0.054
5.431GlyVal: 5.431 ± 0.059
1.29GlyTrp: 1.29 ± 0.03
3.153GlyTyr: 3.153 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
1.901HisAla: 1.901 ± 0.029
0.176HisCys: 0.176 ± 0.009
1.049HisAsp: 1.049 ± 0.024
1.317HisGlu: 1.317 ± 0.026
0.862HisPhe: 0.862 ± 0.021
1.635HisGly: 1.635 ± 0.028
0.611HisHis: 0.611 ± 0.019
1.185HisIle: 1.185 ± 0.021
0.672HisLys: 0.672 ± 0.019
2.114HisLeu: 2.114 ± 0.032
0.506HisMet: 0.506 ± 0.017
0.646HisAsn: 0.646 ± 0.016
1.327HisPro: 1.327 ± 0.025
0.779HisGln: 0.779 ± 0.018
1.366HisArg: 1.366 ± 0.028
1.058HisSer: 1.058 ± 0.022
1.112HisThr: 1.112 ± 0.023
1.371HisVal: 1.371 ± 0.026
0.376HisTrp: 0.376 ± 0.014
0.974HisTyr: 0.974 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.031IleAla: 6.031 ± 0.051
0.483IleCys: 0.483 ± 0.016
3.747IleAsp: 3.747 ± 0.041
4.003IleGlu: 4.003 ± 0.041
1.941IlePhe: 1.941 ± 0.034
4.902IleGly: 4.902 ± 0.058
1.259IleHis: 1.259 ± 0.023
3.328IleIle: 3.328 ± 0.048
2.11IleLys: 2.11 ± 0.035
4.959IleLeu: 4.959 ± 0.058
1.296IleMet: 1.296 ± 0.024
1.968IleAsn: 1.968 ± 0.033
2.65IlePro: 2.65 ± 0.039
2.129IleGln: 2.129 ± 0.032
3.907IleArg: 3.907 ± 0.049
3.451IleSer: 3.451 ± 0.043
3.147IleThr: 3.147 ± 0.046
4.88IleVal: 4.88 ± 0.043
0.694IleTrp: 0.694 ± 0.017
1.984IleTyr: 1.984 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.349LysAla: 3.349 ± 0.045
0.144LysCys: 0.144 ± 0.008
2.018LysAsp: 2.018 ± 0.035
2.711LysGlu: 2.711 ± 0.042
1.045LysPhe: 1.045 ± 0.024
2.685LysGly: 2.685 ± 0.036
0.849LysHis: 0.849 ± 0.021
2.079LysIle: 2.079 ± 0.033
1.845LysLys: 1.845 ± 0.035
4.471LysLeu: 4.471 ± 0.056
1.041LysMet: 1.041 ± 0.021
1.209LysAsn: 1.209 ± 0.025
1.876LysPro: 1.876 ± 0.034
1.977LysGln: 1.977 ± 0.036
2.562LysArg: 2.562 ± 0.036
2.126LysSer: 2.126 ± 0.035
2.126LysThr: 2.126 ± 0.036
2.537LysVal: 2.537 ± 0.032
0.496LysTrp: 0.496 ± 0.016
1.273LysTyr: 1.273 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
10.268LeuAla: 10.268 ± 0.082
0.822LeuCys: 0.822 ± 0.019
5.465LeuAsp: 5.465 ± 0.05
6.654LeuGlu: 6.654 ± 0.061
4.126LeuPhe: 4.126 ± 0.053
7.078LeuGly: 7.078 ± 0.06
2.272LeuHis: 2.272 ± 0.031
5.828LeuIle: 5.828 ± 0.059
3.864LeuLys: 3.864 ± 0.043
12.159LeuLeu: 12.159 ± 0.126
2.622LeuMet: 2.622 ± 0.039
3.02LeuAsn: 3.02 ± 0.038
5.2LeuPro: 5.2 ± 0.055
4.657LeuGln: 4.657 ± 0.049
6.299LeuArg: 6.299 ± 0.066
7.007LeuSer: 7.007 ± 0.065
6.066LeuThr: 6.066 ± 0.055
6.495LeuVal: 6.495 ± 0.053
1.218LeuTrp: 1.218 ± 0.023
3.541LeuTyr: 3.541 ± 0.044
0.0LeuXaa: 0.0 ± 0.0
Met
2.371MetAla: 2.371 ± 0.032
0.137MetCys: 0.137 ± 0.008
1.393MetAsp: 1.393 ± 0.024
1.719MetGlu: 1.719 ± 0.031
0.905MetPhe: 0.905 ± 0.021
1.594MetGly: 1.594 ± 0.026
0.533MetHis: 0.533 ± 0.017
1.732MetIle: 1.732 ± 0.033
1.566MetLys: 1.566 ± 0.027
3.078MetLeu: 3.078 ± 0.038
0.867MetMet: 0.867 ± 0.022
1.177MetAsn: 1.177 ± 0.02
1.194MetPro: 1.194 ± 0.023
1.106MetGln: 1.106 ± 0.018
1.598MetArg: 1.598 ± 0.029
1.735MetSer: 1.735 ± 0.028
1.817MetThr: 1.817 ± 0.031
1.611MetVal: 1.611 ± 0.028
0.241MetTrp: 0.241 ± 0.011
0.724MetTyr: 0.724 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
2.542AsnAla: 2.542 ± 0.039
0.184AsnCys: 0.184 ± 0.01
1.551AsnAsp: 1.551 ± 0.031
2.014AsnGlu: 2.014 ± 0.031
1.092AsnPhe: 1.092 ± 0.024
2.917AsnGly: 2.917 ± 0.046
0.657AsnHis: 0.657 ± 0.017
1.815AsnIle: 1.815 ± 0.03
1.357AsnLys: 1.357 ± 0.027
2.854AsnLeu: 2.854 ± 0.031
0.806AsnMet: 0.806 ± 0.02
1.325AsnAsn: 1.325 ± 0.035
1.737AsnPro: 1.737 ± 0.034
1.179AsnGln: 1.179 ± 0.024
1.979AsnArg: 1.979 ± 0.031
1.718AsnSer: 1.718 ± 0.03
1.788AsnThr: 1.788 ± 0.034
2.132AsnVal: 2.132 ± 0.033
0.495AsnTrp: 0.495 ± 0.017
1.286AsnTyr: 1.286 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
4.165ProAla: 4.165 ± 0.047
0.244ProCys: 0.244 ± 0.01
2.919ProAsp: 2.919 ± 0.04
3.655ProGlu: 3.655 ± 0.036
1.779ProPhe: 1.779 ± 0.026
3.63ProGly: 3.63 ± 0.04
0.979ProHis: 0.979 ± 0.022
2.406ProIle: 2.406 ± 0.032
1.314ProLys: 1.314 ± 0.025
4.43ProLeu: 4.43 ± 0.047
1.052ProMet: 1.052 ± 0.021
1.288ProAsn: 1.288 ± 0.026
1.651ProPro: 1.651 ± 0.029
1.751ProGln: 1.751 ± 0.024
1.796ProArg: 1.796 ± 0.029
2.766ProSer: 2.766 ± 0.04
2.279ProThr: 2.279 ± 0.04
3.356ProVal: 3.356 ± 0.041
0.621ProTrp: 0.621 ± 0.016
1.62ProTyr: 1.62 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
4.371GlnAla: 4.371 ± 0.051
0.204GlnCys: 0.204 ± 0.011
1.788GlnAsp: 1.788 ± 0.028
2.783GlnGlu: 2.783 ± 0.039
1.428GlnPhe: 1.428 ± 0.026
2.811GlnGly: 2.811 ± 0.035
0.888GlnHis: 0.888 ± 0.022
2.264GlnIle: 2.264 ± 0.033
1.26GlnLys: 1.26 ± 0.026
4.966GlnLeu: 4.966 ± 0.056
1.186GlnMet: 1.186 ± 0.023
0.954GlnAsn: 0.954 ± 0.025
2.098GlnPro: 2.098 ± 0.034
2.3GlnGln: 2.3 ± 0.036
2.542GlnArg: 2.542 ± 0.037
2.434GlnSer: 2.434 ± 0.033
2.366GlnThr: 2.366 ± 0.033
2.767GlnVal: 2.767 ± 0.036
0.647GlnTrp: 0.647 ± 0.017
1.411GlnTyr: 1.411 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
4.591ArgAla: 4.591 ± 0.051
0.438ArgCys: 0.438 ± 0.015
2.873ArgAsp: 2.873 ± 0.039
4.611ArgGlu: 4.611 ± 0.058
2.418ArgPhe: 2.418 ± 0.036
3.499ArgGly: 3.499 ± 0.044
1.443ArgHis: 1.443 ± 0.026
4.021ArgIle: 4.021 ± 0.044
2.397ArgLys: 2.397 ± 0.041
7.133ArgLeu: 7.133 ± 0.077
2.048ArgMet: 2.048 ± 0.033
1.79ArgAsn: 1.79 ± 0.031
2.239ArgPro: 2.239 ± 0.032
3.006ArgGln: 3.006 ± 0.04
3.744ArgArg: 3.744 ± 0.062
3.74ArgSer: 3.74 ± 0.046
3.062ArgThr: 3.062 ± 0.037
3.643ArgVal: 3.643 ± 0.041
0.937ArgTrp: 0.937 ± 0.02
2.477ArgTyr: 2.477 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
5.295SerAla: 5.295 ± 0.058
0.43SerCys: 0.43 ± 0.014
3.184SerAsp: 3.184 ± 0.041
3.912SerGlu: 3.912 ± 0.043
2.65SerPhe: 2.65 ± 0.038
5.921SerGly: 5.921 ± 0.06
1.196SerHis: 1.196 ± 0.024
3.622SerIle: 3.622 ± 0.039
2.228SerLys: 2.228 ± 0.034
6.041SerLeu: 6.041 ± 0.062
1.659SerMet: 1.659 ± 0.028
1.863SerAsn: 1.863 ± 0.035
2.622SerPro: 2.622 ± 0.035
2.068SerGln: 2.068 ± 0.029
3.528SerArg: 3.528 ± 0.04
4.06SerSer: 4.06 ± 0.057
3.038SerThr: 3.038 ± 0.044
4.17SerVal: 4.17 ± 0.045
0.976SerTrp: 0.976 ± 0.025
2.411SerTyr: 2.411 ± 0.034
0.0SerXaa: 0.0 ± 0.0
Thr
5.276ThrAla: 5.276 ± 0.06
0.294ThrCys: 0.294 ± 0.01
2.877ThrAsp: 2.877 ± 0.035
3.233ThrGlu: 3.233 ± 0.037
2.146ThrPhe: 2.146 ± 0.036
5.182ThrGly: 5.182 ± 0.141
1.013ThrHis: 1.013 ± 0.023
3.68ThrIle: 3.68 ± 0.046
1.815ThrLys: 1.815 ± 0.033
5.801ThrLeu: 5.801 ± 0.053
1.43ThrMet: 1.43 ± 0.025
1.706ThrAsn: 1.706 ± 0.035
2.736ThrPro: 2.736 ± 0.036
1.618ThrGln: 1.618 ± 0.028
2.63ThrArg: 2.63 ± 0.033
3.179ThrSer: 3.179 ± 0.039
3.076ThrThr: 3.076 ± 0.048
4.522ThrVal: 4.522 ± 0.056
0.719ThrTrp: 0.719 ± 0.019
2.008ThrTyr: 2.008 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
5.932ValAla: 5.932 ± 0.06
0.561ValCys: 0.561 ± 0.016
3.807ValAsp: 3.807 ± 0.044
4.322ValGlu: 4.322 ± 0.045
2.704ValPhe: 2.704 ± 0.034
4.631ValGly: 4.631 ± 0.048
1.422ValHis: 1.422 ± 0.028
4.233ValIle: 4.233 ± 0.046
2.912ValLys: 2.912 ± 0.042
7.013ValLeu: 7.013 ± 0.066
1.854ValMet: 1.854 ± 0.033
2.404ValAsn: 2.404 ± 0.036
3.102ValPro: 3.102 ± 0.041
2.694ValGln: 2.694 ± 0.036
4.104ValArg: 4.104 ± 0.045
4.63ValSer: 4.63 ± 0.052
4.31ValThr: 4.31 ± 0.06
4.968ValVal: 4.968 ± 0.049
1.011ValTrp: 1.011 ± 0.022
2.582ValTyr: 2.582 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.059TrpAla: 1.059 ± 0.021
0.115TrpCys: 0.115 ± 0.007
0.764TrpAsp: 0.764 ± 0.019
0.872TrpGlu: 0.872 ± 0.022
0.547TrpPhe: 0.547 ± 0.015
0.922TrpGly: 0.922 ± 0.02
0.344TrpHis: 0.344 ± 0.013
0.832TrpIle: 0.832 ± 0.019
0.555TrpLys: 0.555 ± 0.016
1.675TrpLeu: 1.675 ± 0.029
0.465TrpMet: 0.465 ± 0.015
0.624TrpAsn: 0.624 ± 0.018
0.506TrpPro: 0.506 ± 0.016
0.763TrpGln: 0.763 ± 0.02
0.905TrpArg: 0.905 ± 0.02
0.975TrpSer: 0.975 ± 0.023
0.865TrpThr: 0.865 ± 0.023
0.864TrpVal: 0.864 ± 0.021
0.21TrpTrp: 0.21 ± 0.011
0.472TrpTyr: 0.472 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.297TyrAla: 3.297 ± 0.034
0.278TyrCys: 0.278 ± 0.01
2.1TyrAsp: 2.1 ± 0.035
2.518TyrGlu: 2.518 ± 0.037
1.364TyrPhe: 1.364 ± 0.025
2.796TyrGly: 2.796 ± 0.033
0.763TyrHis: 0.763 ± 0.019
2.036TyrIle: 2.036 ± 0.03
1.307TyrLys: 1.307 ± 0.023
3.539TyrLeu: 3.539 ± 0.043
0.923TyrMet: 0.923 ± 0.02
1.382TyrAsn: 1.382 ± 0.028
1.721TyrPro: 1.721 ± 0.028
1.163TyrGln: 1.163 ± 0.024
2.596TyrArg: 2.596 ± 0.038
2.025TyrSer: 2.025 ± 0.029
2.076TyrThr: 2.076 ± 0.033
2.619TyrVal: 2.619 ± 0.035
0.539TyrTrp: 0.539 ± 0.018
1.549TyrTyr: 1.549 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6816 proteins (2278485 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski