Streptococcus satellite phage Javan212
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZHC2|A0A4D5ZHC2_9VIRU DNA-binding protein OS=Streptococcus satellite phage Javan212 OX=2558572 GN=JavanS212_0017 PE=4 SV=1
MM1 pKa = 7.59 LVRR4 pKa = 11.84 LVFSDD9 pKa = 3.86 GNTLTRR15 pKa = 11.84 DD16 pKa = 3.01 ISDD19 pKa = 3.46 RR20 pKa = 11.84 TFDD23 pKa = 3.97 MIMSKK28 pKa = 10.21 PYY30 pKa = 10.51 SDD32 pKa = 3.25 TWLHH36 pKa = 6.27 FNGEE40 pKa = 4.09 KK41 pKa = 10.44 VAVLEE46 pKa = 4.53 SIEE49 pKa = 4.13 VLDD52 pKa = 5.05 NEE54 pKa = 4.69 SDD56 pKa = 3.75 FDD58 pKa = 4.28 NEE60 pKa = 4.27 VNKK63 pKa = 9.49 TLAYY67 pKa = 10.57 NEE69 pKa = 4.24 LKK71 pKa = 10.86 KK72 pKa = 10.72 EE73 pKa = 4.12 VSDD76 pKa = 5.2 DD77 pKa = 3.75 DD78 pKa = 3.89
Molecular weight: 9.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.397
IPC2_protein 4.151
IPC_protein 4.088
Toseland 3.884
ProMoST 4.164
Dawson 4.062
Bjellqvist 4.266
Wikipedia 3.999
Rodwell 3.91
Grimsley 3.795
Solomon 4.05
Lehninger 4.012
Nozaki 4.177
DTASelect 4.406
Thurlkill 3.923
EMBOSS 3.999
Sillero 4.202
Patrickios 3.961
IPC_peptide 4.05
IPC2_peptide 4.177
IPC2.peptide.svr19 4.141
Protein with the highest isoelectric point:
>tr|A0A4D5ZG34|A0A4D5ZG34_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan212 OX=2558572 GN=JavanS212_0004 PE=4 SV=1
MM1 pKa = 7.84 LITEE5 pKa = 5.0 NYY7 pKa = 9.63 AKK9 pKa = 10.59 AIRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 9.06 QADD17 pKa = 3.47 MQLTAKK23 pKa = 10.13 QAGEE27 pKa = 4.08 QIGVNHH33 pKa = 5.2 ITYY36 pKa = 10.31 RR37 pKa = 11.84 KK38 pKa = 8.63 LRR40 pKa = 11.84 SGGEE44 pKa = 3.83 VRR46 pKa = 11.84 AKK48 pKa = 10.0 TFEE51 pKa = 4.06 KK52 pKa = 10.75 AMAWLVKK59 pKa = 10.32 DD60 pKa = 3.76 YY61 pKa = 11.63
Molecular weight: 7.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.124
IPC2_protein 9.443
IPC_protein 9.487
Toseland 10.204
ProMoST 9.853
Dawson 10.365
Bjellqvist 9.999
Wikipedia 10.511
Rodwell 10.891
Grimsley 10.423
Solomon 10.394
Lehninger 10.379
Nozaki 10.16
DTASelect 9.999
Thurlkill 10.218
EMBOSS 10.599
Sillero 10.262
Patrickios 10.672
IPC_peptide 10.409
IPC2_peptide 8.478
IPC2.peptide.svr19 8.497
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19
0
19
2936
43
476
154.5
17.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.871 ± 0.536
0.579 ± 0.211
6.267 ± 0.541
8.379 ± 0.668
3.781 ± 0.352
4.598 ± 0.309
1.328 ± 0.222
7.459 ± 0.523
9.775 ± 0.575
9.843 ± 0.789
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.657 ± 0.314
6.676 ± 0.479
2.01 ± 0.205
3.917 ± 0.239
4.019 ± 0.435
6.063 ± 0.526
6.233 ± 0.556
5.688 ± 0.424
1.124 ± 0.112
4.734 ± 0.413
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here