Pseudogymnoascus sp. VKM F-4513 (FW-928)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; unclassified Pseudogymnoascus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8667 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A094BP41|A0A094BP41_9PEZI Chorismate mutase OS=Pseudogymnoascus sp. VKM F-4513 (FW-928) OX=1420907 GN=V494_01924 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84SSVLVLASVVGVALAQKK19 pKa = 10.75ASDD22 pKa = 3.87CSDD25 pKa = 3.41PQKK28 pKa = 11.24AEE30 pKa = 3.98SCVLKK35 pKa = 9.47LTKK38 pKa = 10.38AGLSCQTVLGIKK50 pKa = 9.54SCYY53 pKa = 10.36DD54 pKa = 3.43DD55 pKa = 4.34FCPEE59 pKa = 3.74IPLPDD64 pKa = 4.83EE65 pKa = 4.26INSIIDD71 pKa = 3.27TCEE74 pKa = 3.7NGGTFPGSGSGSGSGSGSGSGTGSGTGSEE103 pKa = 4.46SGTTTSSASQSTSSTNIEE121 pKa = 4.27SDD123 pKa = 3.66CTDD126 pKa = 3.35ADD128 pKa = 3.83AFSDD132 pKa = 4.21CVKK135 pKa = 10.42PLVKK139 pKa = 10.24VDD141 pKa = 3.79TTCKK145 pKa = 9.37VTMQIQQCFKK155 pKa = 9.96TYY157 pKa = 10.26CPQNIDD163 pKa = 4.43RR164 pKa = 11.84IADD167 pKa = 3.77DD168 pKa = 3.59VASCQGSEE176 pKa = 4.08NGSGTGSDD184 pKa = 4.46DD185 pKa = 4.52DD186 pKa = 4.09STPTSSSDD194 pKa = 3.27DD195 pKa = 3.78SEE197 pKa = 4.55GTGISGGGSSTDD209 pKa = 3.28TSSSADD215 pKa = 3.43STGTGVDD222 pKa = 3.26SGSDD226 pKa = 3.53NTSSSSDD233 pKa = 3.35PTNTSTNSDD242 pKa = 3.32PVATDD247 pKa = 3.22NNVNEE252 pKa = 4.37GAGDD256 pKa = 3.84KK257 pKa = 10.9LFAPAGAIIGSIIAVMAWLL276 pKa = 3.98

Molecular weight: 27.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A094BQ96|A0A094BQ96_9PEZI Uncharacterized protein OS=Pseudogymnoascus sp. VKM F-4513 (FW-928) OX=1420907 GN=V494_01724 PE=4 SV=1
MM1 pKa = 7.61PPSAMPHH8 pKa = 6.21HH9 pKa = 6.79AHH11 pKa = 6.56APSPTSHH18 pKa = 7.22AATTAAAAATSSATRR33 pKa = 11.84TPRR36 pKa = 11.84TSSRR40 pKa = 11.84STKK43 pKa = 8.22TPRR46 pKa = 11.84STLSDD51 pKa = 3.28TAAALANSAGASTKK65 pKa = 10.34GGKK68 pKa = 8.89SRR70 pKa = 11.84AARR73 pKa = 11.84APAAATAATARR84 pKa = 11.84RR85 pKa = 11.84AAGMAAVAPAGARR98 pKa = 11.84MGVRR102 pKa = 11.84ACPRR106 pKa = 11.84RR107 pKa = 11.84RR108 pKa = 3.29

Molecular weight: 10.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8667

0

8667

4433224

17

4963

511.5

56.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.947 ± 0.023

1.151 ± 0.009

5.755 ± 0.02

6.411 ± 0.028

3.599 ± 0.016

7.269 ± 0.026

2.214 ± 0.012

4.996 ± 0.018

5.106 ± 0.024

8.674 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.153 ± 0.012

3.753 ± 0.014

5.882 ± 0.028

3.839 ± 0.019

5.802 ± 0.023

8.085 ± 0.028

6.013 ± 0.017

6.2 ± 0.021

1.401 ± 0.009

2.75 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski