Boudabousia tangfeifanii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Boudabousia

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1712 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D9MKF6|A0A1D9MKF6_9ACTO Bifunctional (P)ppGpp synthetase/guanosine-3' 5'-bis(Diphosphate) 3'-pyrophosphohydrolase OS=Boudabousia tangfeifanii OX=1912795 GN=BK816_04520 PE=3 SV=1
MM1 pKa = 7.59HH2 pKa = 6.95FTDD5 pKa = 6.07DD6 pKa = 3.62YY7 pKa = 10.85LLSVRR12 pKa = 11.84DD13 pKa = 4.08LPRR16 pKa = 11.84QPGSFDD22 pKa = 3.1EE23 pKa = 5.97LYY25 pKa = 11.11AQVTLPWEE33 pKa = 4.1NDD35 pKa = 3.18LVKK38 pKa = 10.98LSDD41 pKa = 4.51PEE43 pKa = 4.71PEE45 pKa = 4.18LHH47 pKa = 7.18FMAQSVSDD55 pKa = 4.12GVLLTINGEE64 pKa = 4.27LAWEE68 pKa = 5.32GSCARR73 pKa = 11.84CLQPAEE79 pKa = 4.27GDD81 pKa = 3.43FDD83 pKa = 5.15LSATEE88 pKa = 4.03LFFYY92 pKa = 10.76PEE94 pKa = 3.65AAKK97 pKa = 10.41AAEE100 pKa = 4.47ANTKK104 pKa = 10.57DD105 pKa = 3.7VDD107 pKa = 3.68IDD109 pKa = 3.84PLPTVVDD116 pKa = 3.85DD117 pKa = 4.94QIDD120 pKa = 3.45IEE122 pKa = 4.96AILRR126 pKa = 11.84DD127 pKa = 4.63EE128 pKa = 4.82IMLALPMLPLCDD140 pKa = 4.08EE141 pKa = 4.61DD142 pKa = 6.3CEE144 pKa = 4.67GLCATCGEE152 pKa = 4.28AWADD156 pKa = 3.58LPADD160 pKa = 3.85HH161 pKa = 6.52VHH163 pKa = 7.24AEE165 pKa = 3.94VDD167 pKa = 3.17PRR169 pKa = 11.84LAVLGQLLANQEE181 pKa = 4.15NEE183 pKa = 4.05QSGEE187 pKa = 4.14SPAA190 pKa = 3.95

Molecular weight: 20.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D9MMB9|A0A1D9MMB9_9ACTO Nucleoside triphosphate pyrophosphatase OS=Boudabousia tangfeifanii OX=1912795 GN=BK816_03245 PE=3 SV=1
MM1 pKa = 7.41VKK3 pKa = 9.03RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.4VHH17 pKa = 5.52GFRR20 pKa = 11.84KK21 pKa = 9.95RR22 pKa = 11.84MSTRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.67GRR40 pKa = 11.84ASISAA45 pKa = 3.59

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1712

0

1712

638556

45

3158

373.0

40.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.0 ± 0.076

0.766 ± 0.016

5.374 ± 0.038

6.361 ± 0.056

3.523 ± 0.036

7.647 ± 0.047

1.833 ± 0.027

4.855 ± 0.046

4.711 ± 0.073

10.07 ± 0.096

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.1 ± 0.027

3.475 ± 0.043

5.494 ± 0.082

4.032 ± 0.039

5.315 ± 0.057

5.88 ± 0.044

5.986 ± 0.046

7.641 ± 0.047

1.512 ± 0.023

2.425 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski