Faeces associated gemycircularvirus 19
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A168MGA2|A0A168MGA2_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 19 OX=1843739 PE=3 SV=1
MM1 pKa = 7.4 SAFHH5 pKa = 6.68 FSARR9 pKa = 11.84 YY10 pKa = 9.45 VLLTYY15 pKa = 9.0 PQSGEE20 pKa = 3.86 LSEE23 pKa = 4.35 WAVLDD28 pKa = 4.71 HH29 pKa = 7.1 ISGLGAEE36 pKa = 4.74 CIIGRR41 pKa = 11.84 EE42 pKa = 4.05 DD43 pKa = 3.35 HH44 pKa = 7.18 ADD46 pKa = 3.64 GGTHH50 pKa = 6.04 LHH52 pKa = 5.93 VFADD56 pKa = 4.85 FGRR59 pKa = 11.84 KK60 pKa = 8.21 KK61 pKa = 9.8 QSRR64 pKa = 11.84 RR65 pKa = 11.84 GDD67 pKa = 3.58 YY68 pKa = 10.34 FDD70 pKa = 5.37 VGGKK74 pKa = 9.22 HH75 pKa = 6.11 PNVVPSKK82 pKa = 10.3 GRR84 pKa = 11.84 PEE86 pKa = 4.58 GGWDD90 pKa = 3.41 YY91 pKa = 10.93 ATKK94 pKa = 10.52 DD95 pKa = 3.6 GNVVAGGLGRR105 pKa = 11.84 PGTSGLPKK113 pKa = 10.41 APNPWRR119 pKa = 11.84 EE120 pKa = 3.71 IVGAEE125 pKa = 3.81 GRR127 pKa = 11.84 EE128 pKa = 3.91 EE129 pKa = 4.09 FLDD132 pKa = 4.45 LVRR135 pKa = 11.84 QLDD138 pKa = 3.77 PKK140 pKa = 11.22 SFVLKK145 pKa = 9.09 HH146 pKa = 4.95 QEE148 pKa = 3.38 IVRR151 pKa = 11.84 YY152 pKa = 9.92 ADD154 pKa = 2.87 IFFAEE159 pKa = 4.26 DD160 pKa = 3.23 RR161 pKa = 11.84 EE162 pKa = 4.59 PYY164 pKa = 10.05 VGPDD168 pKa = 4.0 GIRR171 pKa = 11.84 FEE173 pKa = 5.22 LGMVPQLDD181 pKa = 3.24 EE182 pKa = 3.93 WRR184 pKa = 11.84 RR185 pKa = 11.84 EE186 pKa = 4.08 SLGDD190 pKa = 3.43 NPVEE194 pKa = 4.57 GKK196 pKa = 8.11 CTPSLASSGGPGTDD210 pKa = 3.53 LSS212 pKa = 4.01
Molecular weight: 23.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.264
IPC2_protein 5.245
IPC_protein 5.207
Toseland 5.321
ProMoST 5.41
Dawson 5.283
Bjellqvist 5.372
Wikipedia 5.207
Rodwell 5.219
Grimsley 5.308
Solomon 5.283
Lehninger 5.258
Nozaki 5.448
DTASelect 5.614
Thurlkill 5.372
EMBOSS 5.334
Sillero 5.525
Patrickios 4.228
IPC_peptide 5.296
IPC2_peptide 5.537
IPC2.peptide.svr19 5.466
Protein with the highest isoelectric point:
>tr|A0A168MG89|A0A168MG89_9VIRU RepA OS=Faeces associated gemycircularvirus 19 OX=1843739 PE=3 SV=1
MM1 pKa = 7.36 PRR3 pKa = 11.84 YY4 pKa = 9.42 RR5 pKa = 11.84 RR6 pKa = 11.84 YY7 pKa = 9.81 RR8 pKa = 11.84 AQPRR12 pKa = 11.84 LKK14 pKa = 10.36 RR15 pKa = 11.84 RR16 pKa = 11.84 MKK18 pKa = 10.45 KK19 pKa = 10.22 AVLNTTSTKK28 pKa = 10.35 KK29 pKa = 9.95 RR30 pKa = 11.84 DD31 pKa = 3.47 NMTPISNTTALGAPTPNILRR51 pKa = 11.84 NGAVVIKK58 pKa = 10.78 GGDD61 pKa = 3.51 PSSAAGAGVSGAIFVWSPTARR82 pKa = 11.84 PMNDD86 pKa = 2.57 NTNAEE91 pKa = 4.16 NTRR94 pKa = 11.84 VKK96 pKa = 10.5 RR97 pKa = 11.84 DD98 pKa = 3.36 VYY100 pKa = 11.06 HH101 pKa = 6.45 KK102 pKa = 10.78 GFRR105 pKa = 11.84 EE106 pKa = 4.02 KK107 pKa = 11.12 VKK109 pKa = 10.76 VEE111 pKa = 4.49 SNSSHH116 pKa = 5.57 PWIWRR121 pKa = 11.84 RR122 pKa = 11.84 IVVEE126 pKa = 4.15 TKK128 pKa = 10.27 TDD130 pKa = 3.36 DD131 pKa = 3.51 FNYY134 pKa = 10.37 LGEE137 pKa = 5.02 SEE139 pKa = 5.08 DD140 pKa = 4.32 QPTLPPRR147 pKa = 11.84 AFQPYY152 pKa = 7.77 YY153 pKa = 10.27 QGGEE157 pKa = 4.23 GVSRR161 pKa = 11.84 LWYY164 pKa = 8.64 NHH166 pKa = 5.64 YY167 pKa = 11.06 GNRR170 pKa = 11.84 TGPTDD175 pKa = 3.4 TRR177 pKa = 11.84 IDD179 pKa = 3.79 SVLKK183 pKa = 10.0 TIADD187 pKa = 3.82 DD188 pKa = 4.62 LFEE191 pKa = 4.48 GTQNHH196 pKa = 6.73 DD197 pKa = 3.19 WLNTVTAKK205 pKa = 10.5 VDD207 pKa = 3.62 LDD209 pKa = 4.23 RR210 pKa = 11.84 YY211 pKa = 6.69 TLRR214 pKa = 11.84 YY215 pKa = 10.19 DD216 pKa = 3.0 KK217 pKa = 11.09 TRR219 pKa = 11.84 VFRR222 pKa = 11.84 SGNDD226 pKa = 2.85 AGFIKK231 pKa = 9.91 TVNFYY236 pKa = 11.08 HH237 pKa = 7.05 SFEE240 pKa = 3.73 KK241 pKa = 9.92 TMYY244 pKa = 9.72 YY245 pKa = 10.8 DD246 pKa = 3.57 HH247 pKa = 7.83 DD248 pKa = 4.09 EE249 pKa = 4.68 DD250 pKa = 5.88 RR251 pKa = 11.84 NGTNPQSWVCAPALKK266 pKa = 10.77 GMGNVYY272 pKa = 10.15 IIDD275 pKa = 4.26 IIEE278 pKa = 4.29 SGISADD284 pKa = 3.46 DD285 pKa = 3.44 TDD287 pKa = 4.1 YY288 pKa = 11.72 LRR290 pKa = 11.84 ITPEE294 pKa = 3.56 SCAYY298 pKa = 7.48 WHH300 pKa = 6.44 EE301 pKa = 4.3 RR302 pKa = 3.41
Molecular weight: 34.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.671
IPC2_protein 8.668
IPC_protein 8.639
Toseland 9.077
ProMoST 9.092
Dawson 9.428
Bjellqvist 9.224
Wikipedia 9.648
Rodwell 9.545
Grimsley 9.531
Solomon 9.487
Lehninger 9.428
Nozaki 9.18
DTASelect 9.18
Thurlkill 9.238
EMBOSS 9.531
Sillero 9.385
Patrickios 4.571
IPC_peptide 9.472
IPC2_peptide 7.892
IPC2.peptide.svr19 7.728
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
857
212
343
285.7
32.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.301 ± 0.068
0.817 ± 0.068
7.468 ± 0.09
5.951 ± 0.75
4.084 ± 0.474
10.152 ± 1.521
2.684 ± 0.223
4.201 ± 0.374
4.901 ± 0.326
7.118 ± 1.084
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.867 ± 0.335
3.734 ± 1.242
6.534 ± 0.389
2.217 ± 0.099
7.818 ± 0.358
6.301 ± 0.152
4.784 ± 1.791
6.884 ± 0.261
2.334 ± 0.177
3.851 ± 0.625
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here